LOCUS       sequence-463805-        9914 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  RSF1010-based broad host-range plasmid engineered from pXD68Kan2 
            encoding RFP, kanamycin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9914)
  AUTHORS   Dong X, Bae M, Le C, Aguilar Ramos MA, Balskus EP
  TITLE     Enantiocomplementary Gut Bacterial Enzymes Metabolize Dietary 
            Polyphenols.
  JOURNAL   J Am Chem Soc. 2025 Mar 5;147(9):7231-7244. doi: 
            10.1021/jacs.4c09892. Epub 2025 Feb 24.
  PUBMED    39993729
REFERENCE   2  (bases 1 to 9914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.4c09892"; journalName: "J Am Chem Soc"; date: 
            "2025-03-5- 5"; volume: "147"; issue: "9"; pages: "7231-7244"
COMMENT     Sequence Label: pXD80RFP-pXD68Kan2-RFP
FEATURES             Location/Qualifiers
     source          1..9914
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(19..813)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     primer_bind     1300..1318
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    1475..1494
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     CDS             complement(1497..2108)
                     /codon_start=1
                     /gene="cymR from Pseudomonas putida"
                     /product="cumate repressor (Mullick et al., 2006)"
                     /label=CymR
                     /translation="MSPKRRTQAERAMETQGKLIAAALGVLREKGYAGFRIADVPGAAG
                     VSRGAQSHHFPTKLELLLATFEWLYEQITERSRARLAKLKPEDDVIQQMLDDAAEFFLD
                     DDFSISLDLIVAADRDPALREGIQRTVERNRFVVEDMWLGVLVSRGLSRDDAEDILWLI
                     FNSVRGLAVRSLWQKDKERFERVRNSTLEIARERYAKFKR"
     protein_bind    2446..2473
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P2 operator sequence from the p-cmt 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    2488..2515
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P2 operator sequence from the p-cmt 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    2673..2694
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2709..2714,2715..2732,2733..2739)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2747..2763
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             2781..2792
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             2799..3476
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      3538..3565
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      3697..3724
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(4156..5007)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(4994..5833)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(6344..7315)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELSERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     oriT            8554..8641
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     rep_origin      8983..9377
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     terminator      complement(9540..9583)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     oriT            9679..9788
                     /note="incP origin of transfer"
ORIGIN
        1 ggcgggctgg ccggccttct aaaacaattc atccagtaaa atataatatt ttattttctc
       61 ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg acatactgtt cttccccgat
      121 atcctccctg atcgaccgga cgcagaaggc aatgtcatac cacttgtccg ccctgccgct
      181 tctcccaaga tcaataaagc cacttacttt gccatctttc acaaagatgt tgctgtctcc
      241 caggtcgccg tgggaaaaga caagttcctc ttcgggcttt tccgtcttta aaaaatcata
      301 cagctcgcgc ggatctttaa atggagtgtc ttcttcccag ttttcgcaat ccacatcggc
      361 cagatcgtta ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc tattcgtata
      421 gggacaatcc gatatgtcga tggagtgaaa gagcctgatg cactccgcat acagctcgat
      481 aatcttttca gggctttgtt catcttcata ctcttccgag caaaggacgc catcggcctc
      541 actcatgagc agattgctcc agccatcatg ccgttcaaag tgcaggacct ttggaacagg
      601 cagctttcct tccagccata gcatcatgtc cttttcccgt tccacatcat aggtggtccc
      661 tttataccgg ctgtccgtca tttttaaata taggttttca ttttctccca ccagcttata
      721 taccttagca ggagacattc cttccgtatc ttttacgcag cggtattttt cgatcagttt
      781 tttcaattcc ggtgatattc tcattttagc catgatgttt aactttgttt tagggcgact
      841 gccctgctgc gtaacatcgt tgctgctcca taacatcaaa catcgaccca cggcgtaacg
      901 cgcttgctgc ttggatgccc gaggcataga ctgtacccca aaaaaacagt cataacaagc
      961 catgaaaacc gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt
     1021 gcgtgagcgc atacgctact tgcattacag cttacgaacc gaacaggctt atgtccactg
     1081 ggttcgtgcc ttcatccgtt tccacggtgt gcgtcacccg gcaaccttgg gcagcagcga
     1141 agtcgaggca tttctgtcct ggctggcgaa cgagcgcaag gtttcggtct ccacgcattc
     1201 gaaaccggta tcccgccgcc gccgccggat accggtttcg aattgagatt gccacctgac
     1261 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     1321 tttcgtcttc aaggcgaaaa aaccccgccg aagcggggtt ttttgcgaaa tataatgacc
     1381 ctcttgataa cccaagaggg cattttttag gaacggttat ttgattatta atccggcggc
     1441 aactgaaacg attcggatcc tgtattacta ttctgaattc gcggccgctt ctagatctag
     1501 cgcttgaatt tcgcgtaccg ctctcgcgca atttcgagtg tcgagttcct gacacgctca
     1561 aagcgttctt tgtccttctg ccataggcta cgaacagcaa gcccacgcac cgaattgaat
     1621 atcaaccaaa ggatatcttc tgcatcatca cgcgaaagac cacggctcac cagaacacca
     1681 agccacatat cctcgacgac aaaccgattc ctctctaccg tgcgctgaat accctcgcgt
     1741 aacgctgggt cccggtcggc agccacaatc aaatcaaggc tgatagagaa gtcatcgtcg
     1801 aggaaaaatt cggcggcgtc gtccagcatt tgctggatga cgtcatcctc tggcttcaat
     1861 ttcgctaatc gagcccgact gcgttcggtg atctgttcgt aaagccattc aaaagtggca
     1921 agcagaagct caagctttgt cgggaaatga tggctctgcg ctcctctgga gacaccagca
     1981 gcaccgggca catctgcgat ccggaatccc gcgtaacctt tttcccgtaa aacccccagg
     2041 gccgctgcaa tcaacttgcc ctgggtctcc attgcgcgct ctgcctgtgt tcttctcttt
     2101 ggactcatga tcaccatttt ttttactaaa gcttgatctg caatttgaat aataaccact
     2161 cctttgttta tccaccgaac taagttggtg ttttttgaag cttgaattag atatttaaaa
     2221 gtatcatatc taatattata actaaatttt ctaaaaaaaa cattgaagga acggttattt
     2281 gattattaat ccggcttttt tattatttag gcaactgaaa cgattcggat cctgtattac
     2341 tattctattt tgtcaaaata attttattga caacgtctta ttaacgttga tataatttaa
     2401 attttatttg acaaaaatgg gctcgtgttg tacaataaat gtagtaacaa acagacaatc
     2461 tggtctgttt gtacgagtgt tgcggccaac aaacagacaa tctggtctgt ttgtattatg
     2521 attaactaat gtcactcccc gcatggggag tgcgggttga aatagtcgca ggtgctacgc
     2581 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     2641 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     2701 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     2761 caatttcaca catactagag aaagaggaga aatactagat ggcttcctcc gaagacgtta
     2821 tcaaagagtt catgcgtttc aaagttcgta tggaaggttc cgttaacggt cacgagttcg
     2881 aaatcgaagg tgaaggtgaa ggtcgtccgt acgaaggtac ccagaccgct aaactgaaag
     2941 ttaccaaagg tggtccgctg ccgttcgctt gggacatcct gtccccgcag ttccagtacg
     3001 gttccaaagc ttacgttaaa cacccggctg acatcccgga ctacctgaaa ctgtccttcc
     3061 cggaaggttt caaatgggaa cgtgttatga acttcgaaga cggtggtgtt gttaccgtta
     3121 cccaggactc ctccctgcaa gacggtgagt tcatctacaa agttaaactg cgtggtacca
     3181 acttcccgtc cgatggtccg gttatgcaga aaaaaaccat gggttgggaa gcttccaccg
     3241 aacgtatgta cccggaagac ggtgctctga aaggtgaaat caaaatgcgt ctgaaactga
     3301 aagacggtgg tcactacgac gctgaagtta aaaccaccta catggctaaa aaaccggttc
     3361 agctgccggg tgcttacaaa accgacatca aactggacat cacctcccac aacgaagact
     3421 acaccatcgt tgaacagtac gaacgtgctg aaggtcgtca ctccaccggt gcttaacacc
     3481 tgcgactgtc actccccgca tggggagtgc gggttgaaat actagtcgac tcacactggc
     3541 tcaccttcgg gtgggccttt ctgcgtttat atactagaga gagaatataa aaagccagat
     3601 tattaatccg gcttttttat tatttaattc ctgttataaa aaaaggatca attttgaact
     3661 ctctcccaaa gttgatccct taacgatccc tgcaggagaa ggccatcctg acggatggcc
     3721 tttttgaagc tttctagaat cgggggcccc cggggggatt ctagaacaat ggaagtcctc
     3781 gtctcgccct cgaattagcc cgcttaatga gcgggctttt tttgaattaa ttctcgcgag
     3841 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtaag
     3901 ttagcgcgaa ttgcaagctg gccgacgcgc tgggctacgt cttgctggcg ttcgggagca
     3961 gaagagcata catctggaag caaagccagg aaagcggcct atggagctgt gcggcagcgc
     4021 tcagtaggca atttttcaaa atattgttaa gccttttctg agcatggtat ttttcatggt
     4081 attaccaatt agcaggaaaa taagccattg aatataaaag ataaaaatgt cttgtttaca
     4141 atagagtggg gggggtcagc ctgccgcctt gggccgggtg atgtcgtact tgcccgccgc
     4201 gaactcggtt accgtccagc ccagcgcgac cagctccggc aacgcctcgc gcacccgctg
     4261 gcggcgcttg cgcatggtcg aaccactggc ctctgacggc cagacatagc cgcacaaggt
     4321 atctatggaa gccttgccgg ttttgccggg gtcgatccag ccacacagcc gctggtgcag
     4381 caggcgggcg gtttcgctgt ccagcgcccg cacctcgtcc atgctgatgc gcacatgctg
     4441 gccgccaccc atgacggcct gcgcgatcaa ggggttcagg gccacgtaca ggcgcccgtc
     4501 cgcctcgtcg ctggcgtact ccgacagcag ccgaaacccc tgccgcttgc ggccattctg
     4561 ggcgatgatg gataccttcc aaaggcgctc gatgcagtcc tgtatgtgct tgagcgcccc
     4621 accactatcg acctctgccc cgatttcctt tgccagcgcc cgatagctac ctttgaccac
     4681 catggcatca gcggtgacgg cctcccactt gggttccagg aacagccgga gctgccgtcc
     4741 gccttcggtc ttgggttccg ggccaagcac taggccatta ggcccagcca tggccaccag
     4801 cccttgcagg atgcgcagat catcagcgcc cagcggctcc gggccgctga actcgatccg
     4861 cttgccgtcg ccgtagtcat acgtcacgtc cagcttgctg cgcttgcgct cgccccgctt
     4921 gagggcacgg aacaggccgg gggccagaca gtgcgccggg tcgtgtcgga cgtggctgag
     4981 gctgtgcttg ttcttaggct tcaccacggg gcaccccctt gctcttgcgc tgcctctcca
     5041 gcacggcggg cttgagcacc ccgccgtcat gccgcctgaa ccaccgatca gcgaacggtg
     5101 cgccatagtt ggccttgctc acaccgaagc ggacgaagaa ccggcgctgg tcgtcgtcca
     5161 caccccattc ctcggcctcg gcgctggtca tgctcgacag gtaggactgc cagcggatgt
     5221 tatcgaccag taccgagctg ccccggctgg cctgctgctg gtcgcctgcg cccatcatgg
     5281 ccgcgccctt gctggcatgg tgcaggaaca cgatagagca cccggtatcg gcggcgatgg
     5341 cctccatgcg accgatgacc tgggccatgg ggccgctggc gttttcttcc tcgatgtgga
     5401 accggcgcag cgtgtccagc accatcaggc ggcggccctc ggcggcgcgc ttgaggccgt
     5461 cgaaccactc cggggccatg atgttgggca ggctgccgat cagcggctgg atcagcaggc
     5521 cgtcagccac ggcttgccgt tcctcggcgc tgaggtgcgc cccaagggcg tgcaggcggt
     5581 gatgaatggc ggtgggcggg tcttcggcgg gcaggtagat caccgggccg gtgggcagtt
     5641 cgcccacctc cagcagatcc ggcccgcctg caatctgtgc ggccagttgc agggccagca
     5701 tggatttacc ggcaccaccg ggcgacacca gcgccccgac cgtaccggcc accatgttgg
     5761 gcaaaacgta gtccagcggt ggcggcgctg ctgcgaacgc ctccagaata ttgataggct
     5821 tatgggtagc cattgattgc ctcctttgca ggcagttggt ggttaggcgc tggcggggtc
     5881 actacccccg ccctgcgccg ctctgagttc ttccaggcac tcgcgcagcg cctcgtattc
     5941 gtcgtcggtc agccagaact tgcgctgacg catccctttg gccttcatgc gctcggcata
     6001 tcgcgcttgg cgtacagcgt cagggctggc cagcaggtcg ccggtctgct tgtccttttg
     6061 gtctttcata tcagtcaccg agaaacttgc cggggccgaa aggcttgtct tcgcggaaca
     6121 aggacaaggt gcagccgtca aggttaaggc tggccatatc agcgactgaa aagcggccag
     6181 cctcggcctt gtttgacgta taaccaaagc caccgggcaa ccaatagccc ttgtcacttt
     6241 tgatcaggta gaccgaccct gaagcgcttt tttcgtattc cataaaaccc ccttctgtgc
     6301 gtgagtactc atagtataac aggcgtgagt accaacgcaa gcactacatg ctgaaatctg
     6361 gcccgcccct gtccatgcct cgctggcggg gtgccggtgc ccgtgccagc tcggcccgcg
     6421 caagctggac gctgggcaga cccatgacct tgctgacggt gcgctcgatg taatccgctt
     6481 cgtggccggg cttgcgctct gccagcgctg ggctggcctc ggccatggcc ttgccgattt
     6541 cctcggcact gcggccccgg ctggccagct tctgcgcggc gataaagtcg cacttgctga
     6601 ggtcatcacc gaagcgcttg accagcccgg ccatctcgct gcggtactcg tccagcgccg
     6661 tgcgccggtg gcggctaagc tgccgctcgg acagttcgag gctggccagc ctgcgggcct
     6721 tctcctgctg ccgctgggcc tgctcgatct gctggccagc ctgctgcacc agcgccgggc
     6781 cagcggtggc ggtcttgccc ttggattcac gcagcagcac ccacggctga taaccggcgc
     6841 gggtggtgtg cttgtccttg cggttggtga agcccgccaa gcggccatag tggcggctgt
     6901 cggcgctggc cgggtcggcg tcgtactcgc tggccagcgt ccgggcaatc tgcccccgaa
     6961 gttcaccgcc tgcggcgtcg gccaccttga cccatgcctg atagttcttc gggctggttt
     7021 ccactaccag ggcaggctcc cggccctcgg ctttcatgtc atccaggtca aactcgctga
     7081 ggtcgtccac cagcaccaga ccatgccgct cctgctcggc gggcctgata tacacgtcat
     7141 tgccctgggc attcatccgc ttgagccatg gcgtgttctg gagcacttcg gcggctgacc
     7201 attcccggtt catcatctgg ccggtggtgg cgtccctgac gccgatatcg aagcgctcac
     7261 agcccatggc cttgagctgt cggcctatgg cctgcaaagt cctgtcgttc ttcatcgggc
     7321 caccaagcgc agccagatcg agccgtcctc ggttgtcagt ggcgtcaggt cgagcaagag
     7381 caacgatgcg atcagcagca ccaccgtagg catcatggaa gccagcatca cggttagcca
     7441 tagcttccag tgccaccccc gcgacgcgct ccgggcgctc tgcgcggcgc tgctcacctc
     7501 ggcggctacc tcccgcaact ctttggccag ctccacccat gccgcccctg tctggcgctg
     7561 ggctttcagc cactccgccg cctgcgcctc gctggcctgc tgggtctggc tcatgacctg
     7621 ccgggcttcg tcggccagtg tcgccatgct ctgggccagc ggttcgatct gctccgctaa
     7681 ctcgttgatg cctctggatt tcttcactct gtcgattgcg ttcatggtct attgcctccc
     7741 ggtattcctg taagtcgatg atctgggcgt tggcggtgtc gatgttcagg gccacgtctg
     7801 cccggtcggt gcggatgccc cggccttcca tctccaccac gttcggcccc aggtgaacac
     7861 cgggcaggcg ctcgatgccc tgcgcctcaa gtgttctgtg gtcaatgcgg gcgtcgtggc
     7921 ctgcccgctc taatgcccgg ttggcatggt cggcccatgc ctcgcgggtc tgctcaagcc
     7981 atgccttggg cttgagcgct tcggtcttct gtgccccgcc cttctccggg gtcttgccgt
     8041 tgtaccgctt gaaccactga gcggcgggcc gctcgatgcc gtcattgatc cgctcggaga
     8101 tcatcaggtg gcagtgcggg ttctcgccgc caccggcatg gatggccagc gtatacggca
     8161 ggcgctcggc accggtcagg tgctgggcga actcggacgc cagcgccttc tgctggtcga
     8221 gggtcagctc gaccggcagg gcaaattcga cctccttgaa cagccgccca ttggcgcgtt
     8281 catacaggtc ggcagcatcc cagtagtcgg cgggccgctc gacgaactcc ggcatgtgcc
     8341 cggattcggc gtgcaagact tcatccatgt cgcgggcata cttgccttcg cgctggatga
     8401 agtcggcctt ggccctggcc gattggccgc ccgacctgct gccggttttc gccgtaaggt
     8461 gataaatcgc catgctgcct cgctgttgct tttgcttttc ggctccatgc aatggccctc
     8521 ggagagcgca ccgcccgaag ggtggccgtt aggccagttt ctcgaagaga aaccggtaag
     8581 tgcgccctcc cctacaaagt agggtcggga ttgccgccgc tgtgcctcca tgatagccta
     8641 cgagacagca cattaacaat ggggtgtcaa gatggttaag gggagcaaca aggcggcgga
     8701 tcggctggcc aagctcgaag aacaacgagc gcgaatcaat gccgaaattc agcgggtgcg
     8761 ggcaagggaa cagcagcaag agcgcaagaa cgaaacaagg cgcaaggtgc tggtgggggc
     8821 catgattttg gccaaggtga acagcagcga gtggccggag gatcggctca tggcggcaat
     8881 ggatgcgtac cttgaacgcg accacgaccg cgccttgttc ggtctgccgc cacgccagaa
     8941 ggatgagccg ggctgaatga tcgaccgaga caggccctgc ggggctgcac acgcgccccc
     9001 acccttcggg tagggggaaa ggccgctaaa gcggctaaaa gcgctccagc gtatttctgc
     9061 ggggtttggt gtggggttta gcgggctttg cccgcctttc cccctgccgc gcagcggtgg
     9121 ggcggtgtgt agcctagcgc agcgaataga ccagctatcc ggcctctggc cgggcatatt
     9181 gggcaagggc agcagcgccc cacaagggcg ctgataaccg cgcctagtgg attattctta
     9241 gataatcatg gatggatttt tccaacaccc cgccagcccc cgcccctgct gggtttgcag
     9301 gtttgggggc gtgacagtta ttgcaggggt tcgtgacagt tattgcaggg gggcgtgaca
     9361 gttattgcag gggttcgtga cagttagtac gggagtgacg ggcactggct ggcaatgtct
     9421 agcaacggca ggcatttcgg ctgagggtaa aagaactttc cgctaagcga tagactgtat
     9481 gtaaacacag tattgcaagg acgcggaaca tgcctcatgt ggcggccctc gcgagaatta
     9541 attcagataa aaaaaatcct tagctttcgc taaggatgat ttctagcgat gaccctgctg
     9601 attggttcgc tgaccgtaga ctggttggct tggtttcatc agccatccgc ttgccctcat
     9661 ctgttacgcc ggcggtagcc ggccagcctc gcagagcagg attcccgttg agcaccgcca
     9721 ggtgcgaata agggacagtg aagaaggaac acccgctcgc gggtgggcct acttcaccta
     9781 tcctgcccgg ctgacgccgt tggatacacc aaggaaagtc tacacgaacc ctttggcaaa
     9841 atcctgtata tcgtgcgaca tcgcgcgcgc gcgcgcggat gctagcggcg cgccaaaaaa
     9901 aagcccgctc atta
//
