LOCUS       sequence-463806-       20664 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Eggerthella lenta A2 Dadh (Delta-Elen_0478) in 
            Gordonibacter urolithinfaciens DSM 27213.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 20664)
  AUTHORS   Dong X, Bae M, Le C, Aguilar Ramos MA, Balskus EP
  TITLE     Enantiocomplementary Gut Bacterial Enzymes Metabolize Dietary 
            Polyphenols.
  JOURNAL   J Am Chem Soc. 2025 Mar 5;147(9):7231-7244. doi: 
            10.1021/jacs.4c09892. Epub 2025 Feb 24.
  PUBMED    39993729
REFERENCE   2  (bases 1 to 20664)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.4c09892"; journalName: "J Am Chem Soc"; date: 
            "2025-03-5- 5"; volume: "147"; issue: "9"; pages: "7231-7244"
COMMENT     Sequence Label: pXD80ENSF7.8-Dadh-Delta-Elen0478(ElA2)
FEATURES             Location/Qualifiers
     source          1..20664
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(19..813)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     primer_bind     1300..1318
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    1475..1494
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     CDS             complement(3005..3028)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     terminator      14288..14315
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      14447..14474
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(14906..15757)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(15744..16583)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(17094..18065)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELSERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     oriT            19304..19391
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     rep_origin      19733..20127
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     terminator      complement(20290..20333)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     oriT            20429..20538
                     /note="incP origin of transfer"
ORIGIN
        1 ggcgggctgg ccggccttct aaaacaattc atccagtaaa atataatatt ttattttctc
       61 ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg acatactgtt cttccccgat
      121 atcctccctg atcgaccgga cgcagaaggc aatgtcatac cacttgtccg ccctgccgct
      181 tctcccaaga tcaataaagc cacttacttt gccatctttc acaaagatgt tgctgtctcc
      241 caggtcgccg tgggaaaaga caagttcctc ttcgggcttt tccgtcttta aaaaatcata
      301 cagctcgcgc ggatctttaa atggagtgtc ttcttcccag ttttcgcaat ccacatcggc
      361 cagatcgtta ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc tattcgtata
      421 gggacaatcc gatatgtcga tggagtgaaa gagcctgatg cactccgcat acagctcgat
      481 aatcttttca gggctttgtt catcttcata ctcttccgag caaaggacgc catcggcctc
      541 actcatgagc agattgctcc agccatcatg ccgttcaaag tgcaggacct ttggaacagg
      601 cagctttcct tccagccata gcatcatgtc cttttcccgt tccacatcat aggtggtccc
      661 tttataccgg ctgtccgtca tttttaaata taggttttca ttttctccca ccagcttata
      721 taccttagca ggagacattc cttccgtatc ttttacgcag cggtattttt cgatcagttt
      781 tttcaattcc ggtgatattc tcattttagc catgatgttt aactttgttt tagggcgact
      841 gccctgctgc gtaacatcgt tgctgctcca taacatcaaa catcgaccca cggcgtaacg
      901 cgcttgctgc ttggatgccc gaggcataga ctgtacccca aaaaaacagt cataacaagc
      961 catgaaaacc gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt
     1021 gcgtgagcgc atacgctact tgcattacag cttacgaacc gaacaggctt atgtccactg
     1081 ggttcgtgcc ttcatccgtt tccacggtgt gcgtcacccg gcaaccttgg gcagcagcga
     1141 agtcgaggca tttctgtcct ggctggcgaa cgagcgcaag gtttcggtct ccacgcattc
     1201 gaaaccggta tcccgccgcc gccgccggat accggtttcg aattgagatt gccacctgac
     1261 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     1321 tttcgtcttc aaggcgaaaa aaccccgccg aagcggggtt ttttgcgaaa tataatgacc
     1381 ctcttgataa cccaagaggg cattttttag gaacggttat ttgattatta atccggcggc
     1441 aactgaaacg attcggatcc tgtattacta ttctgaattc gcggccgctt ctagatttag
     1501 tacatcagga ccactgggta gcccagctgg tagatcagca cgcgaaacat cacggaggtg
     1561 gccagcgcgc cgatggcggc gacgaccatg agcgtcttgg aggcgcccac cttcttcttc
     1621 gccacgaagg cgcaggccgc agccacgatc acggagacga tggagcccca gaacatgggc
     1681 gctcccgcgc cggacaccat gacggtcatc aggctgtcga cgtgcgtggg ggccgtggtc
     1741 atcgatgtcg ggtcggcgta gtagccgaat tgcgcgatct tcgccatggc gcaggcgatg
     1801 acgaacacca cgtcggccac gatctgcacg agggccgcga tgaacgtcag ctggatgccc
     1861 gtcgcctcga cggcctcgtc cttcttcagg atggagatca gccagacggc cgcggcgccc
     1921 aggaggcacg cgttgcccag atagaacgcc acgagcgcca ggccccacgc gggacgggcc
     1981 ggcatgaggt aggagtgcgc ggtggcgacc atcatgagca cggcaaccgc gagggtcgcc
     2041 caggccagcg ccttgggaac gggcttgccg cctcgaagga ccacgaacca tgcgacgatg
     2101 acgatggcca gcgccacgca tccgatgagc tcctgggtga tgcccgacgt gatgtgcccg
     2161 aagccgttga agatgcgctc ccagtgctcg aggtgcagga aggctccgat gccgcctacc
     2221 accagcgaga cggcggacgt gatgagcgcg gtcatctgca ggttcctgcc ctggcccttg
     2281 agcgccagga tggacatgcc gcccagcgtg ccgcaggtca agcatacgaa cagcgtgaag
     2341 atgacgagag gccactgcgt ttccattact catcacctcc ttcccagtga tggtcgcgta
     2401 ggatgtagga gtacgacggc ttgttgccca cgtcgggcag cttgtgcacg tcggcgtcgg
     2461 tgaacggctc caggaactcc accatcttgc ggcgcttgcc cagcgggtcc tcggagccct
     2521 cgaacgactc gtagccggcc tcggtgtcgc cgatccagcg ggcgttgccg ccgcactggc
     2581 tgacgcactg gggcagctcg ccctgcgaca gcttctgctc gcacagcgtg cacttctcga
     2641 cgaccttctc ctcctcgttg aggtagcgga cgccgtaggg gcaggccatg acgcagaact
     2701 ggcagccgat gcacttctcc ttgtcgatct ggatggagcc gtcctcggcc acgtgcgacg
     2761 cctcggtggg gcacaccttc acgcactcgg ggttctcgca gtgctggcag ctgatgggca
     2821 ggaagtacat gtacacgtcg gggtactgcc ctgatccgcc cgggatgggg ttcggcccca
     2881 cgcgcaccac tttgttccag tagctgccga tgggcacgtt gttgaccgcc ttgcaggcga
     2941 ccgagcacgc caggcagccc acgcaccggt tgaggtcggt gatgatagcc ttcgtcatgg
     3001 ttcagtgatg gtgatggtga tggtgatgct cgagtgcggc ctccctccct tcgtaggtag
     3061 gcagccattc cttcagacgg gggtcgtcgg aggtatggat gatgggcgtg ccggtggagt
     3121 cgcacggcac ggggttgttg aacggcgagt tctcgggcgt ggccttgtag atcttcacct
     3181 ggtaggcgcg caggttggac gtgccggagt gcgggtcctg ggcgtagtgg tcgatcagct
     3241 ggttcacctg ggagaactgc cagccgtggc cggcgtcctt cacctcgggg taccaccacg
     3301 tgtgctcgag gttgatgacg tcttccttca cgccgtagta caggtcagcc acttcgcgga
     3361 tcttgcccca ctcggtttcg atccacaccc agtcgccctg ctcgatgccg tactcggcgg
     3421 ccgtcttcgg gttgatctcc acgcgcggca cgggccacag ctcgcggcac cagggaagct
     3481 ggcgatgctc ggagtggaag tacaccggga tgcgacggcc ggtggtggcg gtcagcgggt
     3541 actccttgat gcggtcgccg tccttcgggc cgtgaggcgg ctcggtgtag gtgggaagcc
     3601 tccactcggg atggtcggga tggtgcgatt ccatgacggt ggaccagatc tcctgcttca
     3661 tcgtcggggt gaaccagccc ggcttccagt cgccgatgcc cttgccggcc gtgtagtcga
     3721 ggcggcccca cacgcggtcg cgtgcgcgca tcgcgccggt ctggtagcgg cggtacgtgc
     3781 cccagttgcg cggctcgatg tccttcgcct gccaccagcc gtgctcctgg aaggccttga
     3841 cgtactcgtc ccacgtcttg tacttgggcg tgacctcggc catcggaacg ccgtggcgct
     3901 cgaacgtggg cttgccgttc tcgacgtgcc acgtggtgta ggtcagctcg tcgtcggtga
     3961 gcagcttgat ggagtcgtcc agctgccagt tgatgtcggg ccactcgttg cccggctcgt
     4021 cggtccactt ccagttcatg cggcgggcga ggtccatgac gatctcggga tcgtacttgg
     4081 cttccgcggg cggctgcacg cacttgaccg tggcgcccat ggcgccggcg gaaccttggc
     4141 tggcgcgcgg cgagttgagc tcgatccaat gggccaccgg caggatgatg tcggcggtgt
     4201 cgttctgcgg cgtgtgccac aggttgaggt ccacgtagaa gtcaagcgag cacagggcct
     4261 cccaggcgcg cgtggtgttc gtctggctca tgaagttgcc ggactcgttc cagagggcgc
     4321 gcaccgggta ggggtcgccc gtgatgacgg cgtcccacag cgaatggctg tcgcaccagt
     4381 actgccacca gcccagaagc gggaacttgt cgatgccgat ctgcttgcgg ttgacttcct
     4441 ccggcggggt ggtggcgccg ggggcccagg cgctgaagcc ctggaggtcg ccgtcgatgg
     4501 ggacgatggt cgggccgcgg ttgccgcccg gggtgtccat gttgccggtg atgcccacga
     4561 ggttgtcgaa ggcgcggcag ttctggatgg cgctgcagaa gtgctcgacc gccagcatgt
     4621 actggatgcc gccgttgccg tagccggtgg acgggtcgat gcgcgtgccg taggccgtgg
     4681 ccgccgcctc gatctcggag gcggggatgc cggtgatctc ggccgccacc tcgggcttgt
     4741 actcggcggc gcgggccttg tagtgctccc acaccggctt gaccttcacg gtcttgccgt
     4801 ccttcagcgt gatctcgaac tcgccttcca gcgcggggtc gatggcgggg tcgaacggca
     4861 tgggatcggg cagccagccc tgcgtctggc ccggcagcag gttgtcctgc gcggcctcgc
     4921 ggccttggcg cgccttctcg gagtcgtagt tctcgccctc ccagaagccg ccggtctcgt
     4981 cgatgacgcc ttcctggtcg gcgttgaacc aggtgaacgc gccgtactcg tgctcgacgc
     5041 cctcggcctt cagcttctcc cagttgttgt cgtacacgag gaacttatag gggctgccgc
     5101 cctccttgat gtcgctttcc ttgagcagcg cggttttcac gtcccagtag gaaccgtcgg
     5161 tgcgcacggt ggggaagccg gagggctcca tgtcctcgca cacgaggaac ggggcgttcg
     5221 tccacttctt cacgtacagc tcgtcgtaga gcttcttctc gatgatgacg ttcgtccagg
     5281 ccagggccag ggcgccgtcg gtgccggggc gcaggtgctg ccagtagtcg gcttccttgc
     5341 ccatgttggc catgcggggg tcgacgctga tgtgcacgtc ggcgcgcgat gccacgtcca
     5401 ccgtggtgcg gcaggagtcg tcgtagttgg acagctcgga cgcgccgccc cactgcacgt
     5461 acactttcgg gcgcgtgatg gtttccatcc acgacatggc gaactgcgac accatggtgg
     5521 tggcgaagtg gcgcgggccc ttgcagatct gccaggcctc aacgttgttc ggcgtttcca
     5581 gcatgttctt caggatggac tccgagtgca tgcaccagat gcgcgacgtg cccacctggc
     5641 aggcgatggc ctcgccgccg tgcttcgcct tgatgtccat gaagttgtcc acgatggact
     5701 gcatggcctc gtcccacgag atgcgctgcc agccgggctc ctcgcccttc gggttcgtgc
     5761 gcttcatggg atagtgcaga cggtcggggt ggtacgccgc ctggatggac gactgcgact
     5821 tcgtgcagca gttgcccatg gactggaacg ctccctcgtc gccctcgacg cgtatggccc
     5881 gcccgttctg gacgatgacc ttgacgccgc actccatctt gccgcagccg cggcagcacg
     5941 ttttcacggc cacggtgtcg ttgccggaca cgctgctgta cgtctcttcg gccagcgcgg
     6001 tgtgcgtcga agcgccgaac gccgcggctg ccgcaccggt gatggcggcg gtcttgacga
     6061 acgtgcgtcg tgacatggtc aggttaccca tactcctcct ccttctttct cctcgttctc
     6121 gtcttgctcg tcttcgttcg tgcgatcggg gaggggggct gccgggtgaa gcctcgggct
     6181 ccgaaccggt gcaaagtccc cgtgtcgctt gggccgacca tacggatgcg agggggtgca
     6241 gagcatcgtc caaaggttgg aaagcaggtt cgaccgcatc ataaattctt atgatcttgt
     6301 gatcatgaaa aattcgatag tgctttgaac tggggaaact ttaaccgtag gtcataaatt
     6361 attctgaaac tagaatagtt tcctcaaaga gggccgctca tcttttccgt ataatcgaac
     6421 ccgtcgacag aggggtcggc gcgagcgacg ttatgtacgg gctcacaggg ggtggggcat
     6481 ggaaaagctt cggagtgcat gggataccgc gttggggacg agcctttccc tcaagcgcat
     6541 cgcgggcgcg gccttgcaat ggctttggat gctcatgatg ttctacagca tcgtgccgta
     6601 cggcttctcg tcggacgtgc gcggctcgct gtacgtcagc ctgatcatat cgctggtggc
     6661 catggtggtg accatgctgt tcgtctccgt catcgtgcgg cgcgagcggg tgagcggcaa
     6721 ccgctccatc gtgctgggat cggccatcat gatgagcgcg ggcagcgtgc tcacgccgtt
     6781 ctccgatcct accacgcccg tcggcatgct ggtgctgggc ctgagcgcgg tcatgaccgg
     6841 cacgggctcg gccgtgctgt tcttgtgctg gatcgagctc gagtcggggc tgggaggccg
     6901 cttggcgctt gtggagcttg cctcgtcgct gtgcatcgcg ttcgtcgtgg ggttcttgct
     6961 gatcgtaggg ccggcgatac ccgtcatcgt gctcatcgtt gtcatgcccg tgctttcggc
     7021 ggcagctctc aggcgcagcg cgcccgatac gccgcagctg ccgcgcgagc ccgagcagcc
     7081 tttgtcgcgc catacggcga ccctgttcgc gaaggcgctt ctgggcgcgg tgctcatcgg
     7141 catgttgatg gggttcttcg acgtggtgtc ggggttcaaa acctacgagg tgcaggacat
     7201 cttcggcacg tacctgtttc tggcgggctt catcggagcg ttggccatct gcctgatagc
     7261 cgtgttcctg cagcgcgaca gcgtgttctt ctcgtaccgc gtctccatgc tgatgctgtg
     7321 cttgggatgc ttgtccacgc cgttcatggc cgacaacaac acgtattcga gcgcgcttat
     7381 cttcggcggc taccactgct tcgtgctggt gctgtgcgtg gtgtgcatcg acgtggcgtc
     7441 cagcttccgc gtgagcccgg cgcggacgat cggcctgggg ttcgtggcgc tgtacggcgg
     7501 cgagaccgca ggccagctgt tcgcccattc gctggaggcg accggggtca gcatgttcga
     7561 cctcacgctg gtcacgctgg tggccgtgtc gctgctgttc atcgcgcatt tgttcctgtt
     7621 cacggagacc gacctcatca aaatcggcat cggcgaggtc agcctgatgg gcacggtgcc
     7681 cgaagaggcc gccgacgacc cggccgaccc ggtggatccg tgcgcgctga tcgcggagcg
     7741 cttcgccctg tcgccgcgcg agaccgacgt gcttccgctg ctgctggagg gccgcaccat
     7801 ctcgcgcatc caggagacgc tgttcatctc ggcgggcacg gtgagcacgc atatccgcca
     7861 catctaccag aagacgggcg tggacaatcg ccaggagctc atcgacctgg cccacgagac
     7921 ggccgcgcag gacgaggcgg tctgaggcga tggagacggc gtatctgcac gagttcgcga
     7981 aggtggccga ttgcggcagc ttcacggccg ccgcgcgcga gctgcatctg acgcagtcga
     8041 cgctgagcaa gcacgtggcc ttgttggagc gcgaattcgg ggccgacctg ttcgtgcgcg
     8101 accgcagcgg cgtgagcctg accgaggcgg gcggcgtgct gtacgcccag gcgcggcaga
     8161 tggagaagct gctgcgcgcc accgagctgc tggtgcgcgc ggcgcgcgac gggcagggtt
     8221 cggcgggcgc gggggttgcg ggcgcgacgg cgggcgcagc ggcgggcggg ggtgccgtcg
     8281 acggctcgcc tgcggccgcg tgcgacacgg cgcttcgctg caagtgccgg ctcgcggccg
     8341 agcgctgcgg gctcgaccgg cgcgagctgg gcgcgctcat cctgtacgtg gaggagcgcg
     8401 gcttcgaggc gatccagggc gagctggcgc tgacgcgcga cgaggtggcc gacgtgctgg
     8461 gcagcgtgta ccgcaagctg ggcgtgggcg acaagcagga ggcgctcgat ttcatccatt
     8521 ccgtttcgga atgatggtag acgcgcccgc ggcggttggg catactgcgt agaagtgaaa
     8581 gcgctatgaa gctggggggc ttcgagcaaa gggcttcatc cgaagaatgg atggagaccg
     8641 aatatgagtg cttggactgc tgcgtccgtc gaagacgcgg cgaacaatct tcttgaagaa
     8701 ctgaatagcg gcgcgatgcg cttgtgcgtg gagcatgacg cgtccgacga cgttgcgccg
     8761 gcgttgctgc gcgcggctcg cgctcgcgtc gaggcggggt cgaacgtgcg cgtcgtgtgc
     8821 gccgacgccg ttgcagctgc gcgctttcgg gcgcttgcgg ccgacgatcc gctgctgggc
     8881 gcgctcgacg tggtatcggt gcgcgagctg gccctctcga ttctaggcga cgcgcgggtc
     8941 ggcgatgccg tgggacgcga cgcgcgcgtg ctggacgaga acgagctggc cgtgctcatg
     9001 gaggacgtga aggtgagcgg cctcaagccg ggccggctgc gcgagatgct caagttcttc
     9061 tacaagagca tcagcgactg cgccgacgat gacgagcgct ggctgatcac cgccgaagag
     9121 cagaccgtgc acgccatcct caccgagaac ctggaggcgc gccgcgcgtt gctgccgtgc
     9181 gaggtttcgt cgctggcgta tcgcggcctt gtgaaagccg gggtcgagcg cgagccgctg
     9241 acgctggtgg cagacgactt cgggtcgctc agcaaggcgt cgcagcgcct cgtgcgccat
     9301 ctggcgacgg acggcctcgt ggcggcgggc cgcacgctgg cctcgtccag ctccgaggag
     9361 ccgtatccgt gcttcgacgg gttccgcgcg cttgccgacg aggccgactg cctggtgacg
     9421 ctggcgtgcg agcggccggc cgcggcgcgc gagtcccgcg cgctggacga cccggcggcc
     9481 gagttcgcgt tcgcggcggc gtccgtggag gagtgcctgg ccgacggctt ccggccaggc
     9541 gacgtggctg tggccgtgcc gaacgccgct tggggcgagc gcatcgccgc cgagctggaa
     9601 gggcgcggca tcgccgtgga gcgcgggttc gacagcggga agatcaaggg cgacccgcgc
     9661 accgagggcc gttacgccga cctcaagctg gccgcgttcc tgcggttgta cctcgatccg
     9721 cacgatttca cgtcgttgcg ctcgtggctg gggttcggcg attggctgct gcgctccgat
     9781 gcgtttttgg agctgatggc gtacgcgcgc gaccatgaga cgaccgtgcc cgaagccgtg
     9841 gcgcagctgc gcacgcagcg cgacgccgat cgctcctcga cgatattcgg gaagttcgac
     9901 ggcccgctcg acgagctgga cgagctgctg cgcgcctgcg aggaagggct gacgcgcgag
     9961 gccgccgtag cgctgttcga agcgcacgga atgccgttgt ccccgcgcca cgtggagctg
    10021 ctgggcgacg accctgcgca tgcggacgtc gagcgcttgg cgcgctgcgg gttcgcgaag
    10081 ccggtggagg atgtcgcgcg cgatgccgtg catgtggtct cctacgcgcg ctgccacggg
    10141 caccatgtgc gcacggtgtt cgtcaccggc ctggtgaacg gcttcttgcc ggcgagcgac
    10201 gccgtggacg acaagttcac catcgaccat cggcgcgtgg cgctcgaacg cgagcgcgcg
    10261 ctgttcctgg acgtgctgtc ctgtgcgagc gaacgagcgg tatgcacgcg gttcgtgcgc
    10321 gaccttttag agaacaccgc gttcacgaag gtgcagacct cgcgcgtgtt cgtgcgcgac
    10381 ggcgagcgct tcgcgcgcgt gacgccgtcg gagttcgcgt cgctgaccga cgaggtgctc
    10441 tctcccgcgc ccgacgcgcc tcgcgagctg ccgacgaagg tgctgtacgc gtcttcgact
    10501 ttgtagctcc aacggtctga cgaccgcgcg aaccttcgct gacttttcct gaccggtgat
    10561 tttgaaatga tgaaaggact gatagatatg agcgatgttg atgtattcgt gatcgatgac
    10621 gatttcgatc ccctggccga cctgagcctg gacgacggcg agcgggacga tgccgaggcg
    10681 gattacctgc cgcccatccc cgatgccgac aagagcgtgg tgcctcccgt ggtgccgttg
    10741 tctgcgaccg agcgcatcga gaagctgctg gcgggcattc ccggccagca gttccgcctg
    10801 ctgcacgccg tggaattctg caccgagccg aagacgatgg acgaggccgt ggccaatttg
    10861 gacgcggcgt atccgaacac gacgagcgtg tacggctcgg cgcaggtggt gcagctgctc
    10921 gagcgcgacg gcgcgctgga gcgcatcgtg gacgaggacg ccgcccatgc ggccgccggc
    10981 gacgctgccg atacgcccgc cgaggacgag ggcttcatct cggtgacgcc cgctccgccg
    11041 tgtcactacc gtgccacgca agcgggcctc gatgccgtcg cggcgcacgt caacgagggc
    11101 ctcgtggcgg agcggatcag cgaagacgag cgctatctgc ccatcttcca gcgcgtgctt
    11161 gagatgtgcg cgcgcgaagg aggctgcccc accaaggagc tggaccaggc ggtggacggc
    11221 gacccgctgt gccaagagcc gcgccgcttc tgcgggttct tccgcggcaa gctggaagaa
    11281 acaggcgcca tcgaatggcg cgacgcctgg accattaccg atctggggcg cagcgtgctc
    11341 gcgtccggct tgttcgccgc cgcttccccg tccgaacgct agagaaggag ccacccatgt
    11401 cccacgatca ggaaaccacc atcgccgacg tgctgcgcgg ccgcatggca acctaccagt
    11461 tcctctcgcg cctgttccgc gtggaggtgg accaggagct gtacgacacg ctgatgtcca
    11521 tgcgcttccc ggcgaacacc ggcaacgcgc tggtggacga aggctaccgg atgatctgcg
    11581 gttatctgag ccaagccgac ggcacggtgc tcaccgagct ggcggtggac tacgtgcgcg
    11641 cgttcatcgg gtcgggcaac gacggcttct cggccgcgta cccgtacgag tcggtgtaca
    11701 cgagcccgaa gcgcctcatg atgcaggacg cccgcgacga ggtgctggtg ctgtaccgcg
    11761 ccttcggtct ggacaagcag gaaagctgga aggagggcga ggaccatatc gcgctggagc
    11821 tggagttcga gcagatccta tgcgagcgcg ctatcagggc gtacgaggcc ggcgacgagg
    11881 acgagtgcct gaagctgctg ctgtcgcagc gcaacttcct ggaggaccac ctgctggcgt
    11941 ggtaccccat gatggcggcc gacttgcaga agttcccgca gaccgacttc tacaagggcc
    12001 tgggcaagct gaccgacggc ttcctgcgca acgaccgcga atttttggac gccgtgctgt
    12061 ctgagaacga agcggattgc catccagagc ggaggccgca ggccgaagcc gaaggatccc
    12121 gtgcggcgtc ggccgaaacc gaagtcgagg ttgcatgaat gaagaagctc aaaacctatc
    12181 ggctgcggtt cctgatcatg ctggcgctgc tggccgtggt ggccgtgggg tacttcacgg
    12241 ccggcggcat cggcaacttc tgcggcatcg gcttcgagtc catcacgctg ctgtgccccc
    12301 taggcgcgct gctggccatg atcgccgagc gcacagccat cccgatggcc gtgatcagcg
    12361 ttgcggtcgt gctgctggtg tgtatcgtgc taggcaaggt gttctgcgcc tgggcgtgcc
    12421 ccgtgcactt cctgtcgcga ggaaggaaga agggggataa gggggacaag gagggtaaag
    12481 ggggcaaggt tgcttcccct tgctccgcgt gcgcttcccc ttgcggcaaa gccaagggca
    12541 tcaagatcga cagccgtcac ggcatcctcg ctgcggcgtt gggctccacg ctcgtcttcg
    12601 gcttcccggt gttctgcctc gtctgcccga tcggcctcac gttcgccacg gtgctgctgg
    12661 tcatgcgcct gttcgcgttc ggcgacgcca cgtggacggt cgtcgccttc ccggccatca
    12721 tcgcgctgga gatcctgctg ctgccgaagt ggtgccagcg cttctgcccg ttgggcgcgt
    12781 tgctttcgct gttcagcggg ctgaaccgga cgttccgccc ccaggtggac gccgagaagt
    12841 gcctgcgcga gggtcgcggc aaggcctgca acctgtgcga gcaggcatgc cccgagggca
    12901 tcaacctgca cgatgtcgcg gcgggcgaga ccacgctcaa cgactgcagc aagtgccgcg
    12961 cgtgcgccga cgtgtgcccc gagcgcgcca tcacgttccc gttgctgccg aagaagcagg
    13021 cgcaggcgac gccgctgccc gtcgacgacg agatcccgga aaggggatag gccatgtccc
    13081 tgatcaacgg cctgctggcc ctgttgggcg agatcgagag cgatgccatc gccgtgcatg
    13141 aggaacgctg catctcggtt cgcaaccgca acgccgactg cctgcgctgc gtggaggcat
    13201 gcacgtcggg cgcgctggcg taccgcgcgg gcgagctgct cgtagagccc gagcgctgca
    13261 tcggatgcgg cacgtgcgcc acggcgtgcc ccacctgcgc catcgagctg cgcaacccga
    13321 cggatgccga gctgacggcg cagctcaagc actccatcgt ggcgacgaaa ggccatccgg
    13381 tcatcgtgtg cgagcaggcg ctcgaggcgg ccaacgtcgc ggccgacgat gcgtcggccg
    13441 cgtgcgcggt gccgtgcctg gggcgcatgg acgagtcggc gctcgtgggg ttggcggcgt
    13501 accgcgcctt cgacgcgacg ctggcctgcg gttcgtgcga gacgtgccca catgctccgg
    13561 gcggcgcgct cgtgcgcgag gtggtggagg gcgcgcgcaa cctgcttgag gcgttcggat
    13621 cctccatgcc catcgacctg gaggaatgcg tgcccgagcg cgtgttggag cccggcgggt
    13681 cgcacggcca ggcaggcagc gacggggtgg ggcggcgcga cttcttccgc tcggccaagg
    13741 acgcatcgac ccgcgcagct ggcgcggcgg tggccgagga gttggggatg gtcgaggccg
    13801 agcccgttcc tgcggcgcat cgcaaagtgg gcgcagatgg gacgctgtcg cagttcgtcc
    13861 ccacgcgccg cgtgcggctg tacaactacc tgcagcacgt ggggcaaccg acggccgacg
    13921 aggtggagac gcgtgtcatc ggcgccgtgt ccatcgatgc ccagaagtgc agttcgtgcc
    13981 gcatgtgcgc ggtgttctgt cccacgggcg ccatcaagaa gctcgacgag ggcggcgtgt
    14041 tcggcatcgt gcaccgtccg agcgcctgca tgcagtgccg cctgtgcgag cacatctgtc
    14101 ccgagcaggc catcgcggtg agcgggcgcg tgcccatcaa gcagttcatg ggcaaggaag
    14161 ccgtgtgctt cgctatgaag cgtccaacct ggaccccgaa caagccctca tcgatgtacg
    14221 ataagctgca cgcgacgctc ggcgaacact tggagatgtg catgttctag actagtcgac
    14281 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagaga gagaatataa
    14341 aaagccagat tattaatccg gcttttttat tatttaattc ctgttataaa aaaaggatca
    14401 attttgaact ctctcccaaa gttgatccct taacgatccc tgcaggagaa ggccatcctg
    14461 acggatggcc tttttgaagc tttctagaat cgggggcccc cggggggatt ctagaacaat
    14521 ggaagtcctc gtctcgccct cgaattagcc cgcttaatga gcgggctttt tttgaattaa
    14581 ttctcgcgag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
    14641 ttaatgtaag ttagcgcgaa ttgcaagctg gccgacgcgc tgggctacgt cttgctggcg
    14701 ttcgggagca gaagagcata catctggaag caaagccagg aaagcggcct atggagctgt
    14761 gcggcagcgc tcagtaggca atttttcaaa atattgttaa gccttttctg agcatggtat
    14821 ttttcatggt attaccaatt agcaggaaaa taagccattg aatataaaag ataaaaatgt
    14881 cttgtttaca atagagtggg gggggtcagc ctgccgcctt gggccgggtg atgtcgtact
    14941 tgcccgccgc gaactcggtt accgtccagc ccagcgcgac cagctccggc aacgcctcgc
    15001 gcacccgctg gcggcgcttg cgcatggtcg aaccactggc ctctgacggc cagacatagc
    15061 cgcacaaggt atctatggaa gccttgccgg ttttgccggg gtcgatccag ccacacagcc
    15121 gctggtgcag caggcgggcg gtttcgctgt ccagcgcccg cacctcgtcc atgctgatgc
    15181 gcacatgctg gccgccaccc atgacggcct gcgcgatcaa ggggttcagg gccacgtaca
    15241 ggcgcccgtc cgcctcgtcg ctggcgtact ccgacagcag ccgaaacccc tgccgcttgc
    15301 ggccattctg ggcgatgatg gataccttcc aaaggcgctc gatgcagtcc tgtatgtgct
    15361 tgagcgcccc accactatcg acctctgccc cgatttcctt tgccagcgcc cgatagctac
    15421 ctttgaccac catggcatca gcggtgacgg cctcccactt gggttccagg aacagccgga
    15481 gctgccgtcc gccttcggtc ttgggttccg ggccaagcac taggccatta ggcccagcca
    15541 tggccaccag cccttgcagg atgcgcagat catcagcgcc cagcggctcc gggccgctga
    15601 actcgatccg cttgccgtcg ccgtagtcat acgtcacgtc cagcttgctg cgcttgcgct
    15661 cgccccgctt gagggcacgg aacaggccgg gggccagaca gtgcgccggg tcgtgtcgga
    15721 cgtggctgag gctgtgcttg ttcttaggct tcaccacggg gcaccccctt gctcttgcgc
    15781 tgcctctcca gcacggcggg cttgagcacc ccgccgtcat gccgcctgaa ccaccgatca
    15841 gcgaacggtg cgccatagtt ggccttgctc acaccgaagc ggacgaagaa ccggcgctgg
    15901 tcgtcgtcca caccccattc ctcggcctcg gcgctggtca tgctcgacag gtaggactgc
    15961 cagcggatgt tatcgaccag taccgagctg ccccggctgg cctgctgctg gtcgcctgcg
    16021 cccatcatgg ccgcgccctt gctggcatgg tgcaggaaca cgatagagca cccggtatcg
    16081 gcggcgatgg cctccatgcg accgatgacc tgggccatgg ggccgctggc gttttcttcc
    16141 tcgatgtgga accggcgcag cgtgtccagc accatcaggc ggcggccctc ggcggcgcgc
    16201 ttgaggccgt cgaaccactc cggggccatg atgttgggca ggctgccgat cagcggctgg
    16261 atcagcaggc cgtcagccac ggcttgccgt tcctcggcgc tgaggtgcgc cccaagggcg
    16321 tgcaggcggt gatgaatggc ggtgggcggg tcttcggcgg gcaggtagat caccgggccg
    16381 gtgggcagtt cgcccacctc cagcagatcc ggcccgcctg caatctgtgc ggccagttgc
    16441 agggccagca tggatttacc ggcaccaccg ggcgacacca gcgccccgac cgtaccggcc
    16501 accatgttgg gcaaaacgta gtccagcggt ggcggcgctg ctgcgaacgc ctccagaata
    16561 ttgataggct tatgggtagc cattgattgc ctcctttgca ggcagttggt ggttaggcgc
    16621 tggcggggtc actacccccg ccctgcgccg ctctgagttc ttccaggcac tcgcgcagcg
    16681 cctcgtattc gtcgtcggtc agccagaact tgcgctgacg catccctttg gccttcatgc
    16741 gctcggcata tcgcgcttgg cgtacagcgt cagggctggc cagcaggtcg ccggtctgct
    16801 tgtccttttg gtctttcata tcagtcaccg agaaacttgc cggggccgaa aggcttgtct
    16861 tcgcggaaca aggacaaggt gcagccgtca aggttaaggc tggccatatc agcgactgaa
    16921 aagcggccag cctcggcctt gtttgacgta taaccaaagc caccgggcaa ccaatagccc
    16981 ttgtcacttt tgatcaggta gaccgaccct gaagcgcttt tttcgtattc cataaaaccc
    17041 ccttctgtgc gtgagtactc atagtataac aggcgtgagt accaacgcaa gcactacatg
    17101 ctgaaatctg gcccgcccct gtccatgcct cgctggcggg gtgccggtgc ccgtgccagc
    17161 tcggcccgcg caagctggac gctgggcaga cccatgacct tgctgacggt gcgctcgatg
    17221 taatccgctt cgtggccggg cttgcgctct gccagcgctg ggctggcctc ggccatggcc
    17281 ttgccgattt cctcggcact gcggccccgg ctggccagct tctgcgcggc gataaagtcg
    17341 cacttgctga ggtcatcacc gaagcgcttg accagcccgg ccatctcgct gcggtactcg
    17401 tccagcgccg tgcgccggtg gcggctaagc tgccgctcgg acagttcgag gctggccagc
    17461 ctgcgggcct tctcctgctg ccgctgggcc tgctcgatct gctggccagc ctgctgcacc
    17521 agcgccgggc cagcggtggc ggtcttgccc ttggattcac gcagcagcac ccacggctga
    17581 taaccggcgc gggtggtgtg cttgtccttg cggttggtga agcccgccaa gcggccatag
    17641 tggcggctgt cggcgctggc cgggtcggcg tcgtactcgc tggccagcgt ccgggcaatc
    17701 tgcccccgaa gttcaccgcc tgcggcgtcg gccaccttga cccatgcctg atagttcttc
    17761 gggctggttt ccactaccag ggcaggctcc cggccctcgg ctttcatgtc atccaggtca
    17821 aactcgctga ggtcgtccac cagcaccaga ccatgccgct cctgctcggc gggcctgata
    17881 tacacgtcat tgccctgggc attcatccgc ttgagccatg gcgtgttctg gagcacttcg
    17941 gcggctgacc attcccggtt catcatctgg ccggtggtgg cgtccctgac gccgatatcg
    18001 aagcgctcac agcccatggc cttgagctgt cggcctatgg cctgcaaagt cctgtcgttc
    18061 ttcatcgggc caccaagcgc agccagatcg agccgtcctc ggttgtcagt ggcgtcaggt
    18121 cgagcaagag caacgatgcg atcagcagca ccaccgtagg catcatggaa gccagcatca
    18181 cggttagcca tagcttccag tgccaccccc gcgacgcgct ccgggcgctc tgcgcggcgc
    18241 tgctcacctc ggcggctacc tcccgcaact ctttggccag ctccacccat gccgcccctg
    18301 tctggcgctg ggctttcagc cactccgccg cctgcgcctc gctggcctgc tgggtctggc
    18361 tcatgacctg ccgggcttcg tcggccagtg tcgccatgct ctgggccagc ggttcgatct
    18421 gctccgctaa ctcgttgatg cctctggatt tcttcactct gtcgattgcg ttcatggtct
    18481 attgcctccc ggtattcctg taagtcgatg atctgggcgt tggcggtgtc gatgttcagg
    18541 gccacgtctg cccggtcggt gcggatgccc cggccttcca tctccaccac gttcggcccc
    18601 aggtgaacac cgggcaggcg ctcgatgccc tgcgcctcaa gtgttctgtg gtcaatgcgg
    18661 gcgtcgtggc ctgcccgctc taatgcccgg ttggcatggt cggcccatgc ctcgcgggtc
    18721 tgctcaagcc atgccttggg cttgagcgct tcggtcttct gtgccccgcc cttctccggg
    18781 gtcttgccgt tgtaccgctt gaaccactga gcggcgggcc gctcgatgcc gtcattgatc
    18841 cgctcggaga tcatcaggtg gcagtgcggg ttctcgccgc caccggcatg gatggccagc
    18901 gtatacggca ggcgctcggc accggtcagg tgctgggcga actcggacgc cagcgccttc
    18961 tgctggtcga gggtcagctc gaccggcagg gcaaattcga cctccttgaa cagccgccca
    19021 ttggcgcgtt catacaggtc ggcagcatcc cagtagtcgg cgggccgctc gacgaactcc
    19081 ggcatgtgcc cggattcggc gtgcaagact tcatccatgt cgcgggcata cttgccttcg
    19141 cgctggatga agtcggcctt ggccctggcc gattggccgc ccgacctgct gccggttttc
    19201 gccgtaaggt gataaatcgc catgctgcct cgctgttgct tttgcttttc ggctccatgc
    19261 aatggccctc ggagagcgca ccgcccgaag ggtggccgtt aggccagttt ctcgaagaga
    19321 aaccggtaag tgcgccctcc cctacaaagt agggtcggga ttgccgccgc tgtgcctcca
    19381 tgatagccta cgagacagca cattaacaat ggggtgtcaa gatggttaag gggagcaaca
    19441 aggcggcgga tcggctggcc aagctcgaag aacaacgagc gcgaatcaat gccgaaattc
    19501 agcgggtgcg ggcaagggaa cagcagcaag agcgcaagaa cgaaacaagg cgcaaggtgc
    19561 tggtgggggc catgattttg gccaaggtga acagcagcga gtggccggag gatcggctca
    19621 tggcggcaat ggatgcgtac cttgaacgcg accacgaccg cgccttgttc ggtctgccgc
    19681 cacgccagaa ggatgagccg ggctgaatga tcgaccgaga caggccctgc ggggctgcac
    19741 acgcgccccc acccttcggg tagggggaaa ggccgctaaa gcggctaaaa gcgctccagc
    19801 gtatttctgc ggggtttggt gtggggttta gcgggctttg cccgcctttc cccctgccgc
    19861 gcagcggtgg ggcggtgtgt agcctagcgc agcgaataga ccagctatcc ggcctctggc
    19921 cgggcatatt gggcaagggc agcagcgccc cacaagggcg ctgataaccg cgcctagtgg
    19981 attattctta gataatcatg gatggatttt tccaacaccc cgccagcccc cgcccctgct
    20041 gggtttgcag gtttgggggc gtgacagtta ttgcaggggt tcgtgacagt tattgcaggg
    20101 gggcgtgaca gttattgcag gggttcgtga cagttagtac gggagtgacg ggcactggct
    20161 ggcaatgtct agcaacggca ggcatttcgg ctgagggtaa aagaactttc cgctaagcga
    20221 tagactgtat gtaaacacag tattgcaagg acgcggaaca tgcctcatgt ggcggccctc
    20281 gcgagaatta attcagataa aaaaaatcct tagctttcgc taaggatgat ttctagcgat
    20341 gaccctgctg attggttcgc tgaccgtaga ctggttggct tggtttcatc agccatccgc
    20401 ttgccctcat ctgttacgcc ggcggtagcc ggccagcctc gcagagcagg attcccgttg
    20461 agcaccgcca ggtgcgaata agggacagtg aagaaggaac acccgctcgc gggtgggcct
    20521 acttcaccta tcctgcccgg ctgacgccgt tggatacacc aaggaaagtc tacacgaacc
    20581 ctttggcaaa atcctgtata tcgtgcgaca tcgcgcgcgc gcgcgcggat gctagcggcg
    20641 cgccaaaaaa aagcccgctc atta
//
