LOCUS       sequence-463807-       20549 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Eggerthella lenta A2 Dadh (Delta-Elen_0477) in 
            Gordonibacter urolithinfaciens DSM 27213.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 20549)
  AUTHORS   Dong X, Bae M, Le C, Aguilar Ramos MA, Balskus EP
  TITLE     Enantiocomplementary Gut Bacterial Enzymes Metabolize Dietary 
            Polyphenols.
  JOURNAL   J Am Chem Soc. 2025 Mar 5;147(9):7231-7244. doi: 
            10.1021/jacs.4c09892. Epub 2025 Feb 24.
  PUBMED    39993729
REFERENCE   2  (bases 1 to 20549)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.4c09892"; journalName: "J Am Chem Soc"; date: 
            "2025-03-5- 5"; volume: "147"; issue: "9"; pages: "7231-7244"
COMMENT     Sequence Label: pXD80ENSF7.7-Dadh-Delta-Elen0477(ElA2)
FEATURES             Location/Qualifiers
     source          1..20549
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(19..813)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     primer_bind     1300..1318
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    1475..1494
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     CDS             complement(3005..3028)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     terminator      14173..14200
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      14332..14359
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(14791..15642)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(15629..16468)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(16979..17950)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELSERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     oriT            19189..19276
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     rep_origin      19618..20012
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     terminator      complement(20175..20218)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     oriT            20314..20423
                     /note="incP origin of transfer"
ORIGIN
        1 ggcgggctgg ccggccttct aaaacaattc atccagtaaa atataatatt ttattttctc
       61 ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg acatactgtt cttccccgat
      121 atcctccctg atcgaccgga cgcagaaggc aatgtcatac cacttgtccg ccctgccgct
      181 tctcccaaga tcaataaagc cacttacttt gccatctttc acaaagatgt tgctgtctcc
      241 caggtcgccg tgggaaaaga caagttcctc ttcgggcttt tccgtcttta aaaaatcata
      301 cagctcgcgc ggatctttaa atggagtgtc ttcttcccag ttttcgcaat ccacatcggc
      361 cagatcgtta ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc tattcgtata
      421 gggacaatcc gatatgtcga tggagtgaaa gagcctgatg cactccgcat acagctcgat
      481 aatcttttca gggctttgtt catcttcata ctcttccgag caaaggacgc catcggcctc
      541 actcatgagc agattgctcc agccatcatg ccgttcaaag tgcaggacct ttggaacagg
      601 cagctttcct tccagccata gcatcatgtc cttttcccgt tccacatcat aggtggtccc
      661 tttataccgg ctgtccgtca tttttaaata taggttttca ttttctccca ccagcttata
      721 taccttagca ggagacattc cttccgtatc ttttacgcag cggtattttt cgatcagttt
      781 tttcaattcc ggtgatattc tcattttagc catgatgttt aactttgttt tagggcgact
      841 gccctgctgc gtaacatcgt tgctgctcca taacatcaaa catcgaccca cggcgtaacg
      901 cgcttgctgc ttggatgccc gaggcataga ctgtacccca aaaaaacagt cataacaagc
      961 catgaaaacc gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt
     1021 gcgtgagcgc atacgctact tgcattacag cttacgaacc gaacaggctt atgtccactg
     1081 ggttcgtgcc ttcatccgtt tccacggtgt gcgtcacccg gcaaccttgg gcagcagcga
     1141 agtcgaggca tttctgtcct ggctggcgaa cgagcgcaag gtttcggtct ccacgcattc
     1201 gaaaccggta tcccgccgcc gccgccggat accggtttcg aattgagatt gccacctgac
     1261 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     1321 tttcgtcttc aaggcgaaaa aaccccgccg aagcggggtt ttttgcgaaa tataatgacc
     1381 ctcttgataa cccaagaggg cattttttag gaacggttat ttgattatta atccggcggc
     1441 aactgaaacg attcggatcc tgtattacta ttctgaattc gcggccgctt ctagatttag
     1501 tacatcagga ccactgggta gcccagctgg tagatcagca cgcgaaacat cacggaggtg
     1561 gccagcgcgc cgatggcggc gacgaccatg agcgtcttgg aggcgcccac cttcttcttc
     1621 gccacgaagg cgcaggccgc agccacgatc acggagacga tggagcccca gaacatgggc
     1681 gctcccgcgc cggacaccat gacggtcatc aggctgtcga cgtgcgtggg ggccgtggtc
     1741 atcgatgtcg ggtcggcgta gtagccgaat tgcgcgatct tcgccatggc gcaggcgatg
     1801 acgaacacca cgtcggccac gatctgcacg agggccgcga tgaacgtcag ctggatgccc
     1861 gtcgcctcga cggcctcgtc cttcttcagg atggagatca gccagacggc cgcggcgccc
     1921 aggaggcacg cgttgcccag atagaacgcc acgagcgcca ggccccacgc gggacgggcc
     1981 ggcatgaggt aggagtgcgc ggtggcgacc atcatgagca cggcaaccgc gagggtcgcc
     2041 caggccagcg ccttgggaac gggcttgccg cctcgaagga ccacgaacca tgcgacgatg
     2101 acgatggcca gcgccacgca tccgatgagc tcctgggtga tgcccgacgt gatgtgcccg
     2161 aagccgttga agatgcgctc ccagtgctcg aggtgcagga aggctccgat gccgcctacc
     2221 accagcgaga cggcggacgt gatgagcgcg gtcatctgca ggttcctgcc ctggcccttg
     2281 agcgccagga tggacatgcc gcccagcgtg ccgcaggtca agcatacgaa cagcgtgaag
     2341 atgacgagag gccactgcgt ttccattact catcacctcc ttcccagtga tggtcgcgta
     2401 ggatgtagga gtacgacggc ttgttgccca cgtcgggcag cttgtgcacg tcggcgtcgg
     2461 tgaacggctc caggaactcc accatcttgc ggcgcttgcc cagcgggtcc tcggagccct
     2521 cgaacgactc gtagccggcc tcggtgtcgc cgatccagcg ggcgttgccg ccgcactggc
     2581 tgacgcactg gggcagctcg ccctgcgaca gcttctgctc gcacagcgtg cacttctcga
     2641 cgaccttctc ctcctcgttg aggtagcgga cgccgtaggg gcaggccatg acgcagaact
     2701 ggcagccgat gcacttctcc ttgtcgatct ggatggagcc gtcctcggcc acgtgcgacg
     2761 cctcggtggg gcacaccttc acgcactcgg ggttctcgca gtgctggcag ctgatgggca
     2821 ggaagtacat gtacacgtcg gggtactgcc ctgatccgcc cgggatgggg ttcggcccca
     2881 cgcgcaccac tttgttccag tagctgccga tgggcacgtt gttgaccgcc ttgcaggcga
     2941 ccgagcacgc caggcagccc acgcaccggt tgaggtcggt gatgatagcc ttcgtcatgg
     3001 ttcagtgatg gtgatggtga tggtgatgct cgagtgcggc ctccctccct tcgtaggtag
     3061 gcagccattc cttcagacgg gggtcgtcgg aggtatggat gatgggcgtg ccggtggagt
     3121 cgcacggcac ggggttgttg aacggcgagt tctcgggcgt ggccttgtag atcttcacct
     3181 ggtaggcgcg caggttggac gtgccggagt gcgggtcctg ggcgtagtgg tcgatcagct
     3241 ggttcacctg ggagaactgc cagccgtggc cggcgtcctt cacctcgggg taccaccacg
     3301 tgtgctcgag gttgatgacg tcttccttca cgccgtagta caggtcagcc acttcgcgga
     3361 tcttgcccca ctcggtttcg atccacaccc agtcgccctg ctcgatgccg tactcggcgg
     3421 ccgtcttcgg gttgatctcc acgcgcggca cgggccacag ctcgcggcac cagggaagct
     3481 ggcgatgctc ggagtggaag tacaccggga tgcgacggcc ggtggtggcg gtcagcgggt
     3541 actccttgat gcggtcgccg tccttcgggc cgtgaggcgg ctcggtgtag gtgggaagcc
     3601 tccactcggg atggtcggga tggtgcgatt ccatgacggt ggaccagatc tcctgcttca
     3661 tcgtcggggt gaaccagccc ggcttccagt cgccgatgcc cttgccggcc gtgtagtcga
     3721 ggcggcccca cacgcggtcg cgtgcgcgca tcgcgccggt ctggtagcgg cggtacgtgc
     3781 cccagttgcg cggctcgatg tccttcgcct gccaccagcc gtgctcctgg aaggccttga
     3841 cgtactcgtc ccacgtcttg tacttgggcg tgacctcggc catcggaacg ccgtggcgct
     3901 cgaacgtggg cttgccgttc tcgacgtgcc acgtggtgta ggtcagctcg tcgtcggtga
     3961 gcagcttgat ggagtcgtcc agctgccagt tgatgtcggg ccactcgttg cccggctcgt
     4021 cggtccactt ccagttcatg cggcgggcga ggtccatgac gatctcggga tcgtacttgg
     4081 cttccgcggg cggctgcacg cacttgaccg tggcgcccat ggcgccggcg gaaccttggc
     4141 tggcgcgcgg cgagttgagc tcgatccaat gggccaccgg caggatgatg tcggcggtgt
     4201 cgttctgcgg cgtgtgccac aggttgaggt ccacgtagaa gtcaagcgag cacagggcct
     4261 cccaggcgcg cgtggtgttc gtctggctca tgaagttgcc ggactcgttc cagagggcgc
     4321 gcaccgggta ggggtcgccc gtgatgacgg cgtcccacag cgaatggctg tcgcaccagt
     4381 actgccacca gcccagaagc gggaacttgt cgatgccgat ctgcttgcgg ttgacttcct
     4441 ccggcggggt ggtggcgccg ggggcccagg cgctgaagcc ctggaggtcg ccgtcgatgg
     4501 ggacgatggt cgggccgcgg ttgccgcccg gggtgtccat gttgccggtg atgcccacga
     4561 ggttgtcgaa ggcgcggcag ttctggatgg cgctgcagaa gtgctcgacc gccagcatgt
     4621 actggatgcc gccgttgccg tagccggtgg acgggtcgat gcgcgtgccg taggccgtgg
     4681 ccgccgcctc gatctcggag gcggggatgc cggtgatctc ggccgccacc tcgggcttgt
     4741 actcggcggc gcgggccttg tagtgctccc acaccggctt gaccttcacg gtcttgccgt
     4801 ccttcagcgt gatctcgaac tcgccttcca gcgcggggtc gatggcgggg tcgaacggca
     4861 tgggatcggg cagccagccc tgcgtctggc ccggcagcag gttgtcctgc gcggcctcgc
     4921 ggccttggcg cgccttctcg gagtcgtagt tctcgccctc ccagaagccg ccggtctcgt
     4981 cgatgacgcc ttcctggtcg gcgttgaacc aggtgaacgc gccgtactcg tgctcgacgc
     5041 cctcggcctt cagcttctcc cagttgttgt cgtacacgag gaacttatag gggctgccgc
     5101 cctccttgat gtcgctttcc ttgagcagcg cggttttcac gtcccagtag gaaccgtcgg
     5161 tgcgcacggt ggggaagccg gagggctcca tgtcctcgca cacgaggaac ggggcgttcg
     5221 tccacttctt cacgtacagc tcgtcgtaga gcttcttctc gatgatgacg ttcgtccagg
     5281 ccagggccag ggcgccgtcg gtgccggggc gcaggtgctg ccagtagtcg gcttccttgc
     5341 ccatgttggc catgcggggg tcgacgctga tgtgcacgtc ggcgcgcgat gccacgtcca
     5401 ccgtggtgcg gcaggagtcg tcgtagttgg acagctcgga cgcgccgccc cactgcacgt
     5461 acactttcgg gcgcgtgatg gtttccatcc acgacatggc gaactgcgac accatggtgg
     5521 tggcgaagtg gcgcgggccc ttgcagatct gccaggcctc aacgttgttc ggcgtttcca
     5581 gcatgttctt caggatggac tccgagtgca tgcaccagat gcgcgacgtg cccacctggc
     5641 aggcgatggc ctcgccgccg tgcttcgcct tgatgtccat gaagttgtcc acgatggact
     5701 gcatggcctc gtcccacgag atgcgctgcc agccgggctc ctcgcccttc gggttcgtgc
     5761 gcttcatggg atagtgcaga cggtcggggt ggtacgccgc ctggatggac gactgcgact
     5821 tcgtgcagca gttgcccatg gactggaacg ctccctcgtc gccctcgacg cgtatggccc
     5881 gcccgttctg gacgatgacc ttgacgccgc actccatctt gccgcagccg cggcagcacg
     5941 ttttcacggc cacggtgtcg ttgccggaca cgctgctgta cgtctcttcg gccagcgcgg
     6001 tgtgcgtcga agcgccgaac gccgcggctg ccgcaccggt gatggcggcg gtcttgacga
     6061 acgtgcgtcg tgacatggtc aggttaccca tactcctcct ccttctttct cctcgttctc
     6121 gtcttgctcg tcttcgttcg tgcgatcggg gaggggggct gccgggtgaa gcctcgggct
     6181 ccgaaccggt gcaaagtccc cgtgtcgctt gggccgacca tacggatgcg agggggtgca
     6241 gagcatcgtc caaaggttgg aaagcaggtt cgaccgcatc ataaattctt atgatcttgt
     6301 gatcatgaaa aattcgatag tgctttgaac tggggaaact ttaaccgtag gtcataaatt
     6361 attctgaaac tagaatagtt tcctcaaaga gggccgctca tcttttccgt ataatcgaac
     6421 ccgtcgacag aggggtcggc gcgagcgacg ttatgtacgg gctcacaggg ggtggggcat
     6481 ggaaaagctt cggagtgcat gggataccgc gttggggacg agcctttccc tcaagcgcat
     6541 cgcgggcgcg gccttgcaat ggctttggat gctcatgatg ttctacagca tcgtgccgta
     6601 cggcttctcg tcggacgtgc gcggctcgct gtacgtcagc ctgatcatat cgctggtggc
     6661 catggtggtg accatgctgt tcgtctccgt catcgtgcgg cgcgagcggg tgagcggcaa
     6721 ccgctccatc gtgctgggat cggccatcat gatgagcgcg ggcagcgtgc tcacgccgtt
     6781 ctccgatcct accacgcccg tcggcatgct ggtgctgggc ctgagcgcgg tcatgaccgg
     6841 cacgggctcg gccgtgctgt tcttgtgctg gatcgagctc gagtcggggc tgggaggccg
     6901 cttggcgctt gtggagcttg cctcgtcgct gtgcatcgcg ttcgtcgtgg ggttcttgct
     6961 gatcgtaggg ccggcgatac ccgtcatcgt gctcatcgtt gtcatgcccg tgctttcggc
     7021 ggcagctctc aggcgcagcg cgcccgatac gccgcagctg ccgcgcgagc ccgagcagcc
     7081 tttgtcgcgc catacggcga ccctgttcgc gaaggcgctt ctgggcgcgg tgctcatcgg
     7141 catgttgatg gggttcttcg acgtggtgtc ggggttcaaa acctacgagg tgcaggacat
     7201 cttcggcacg tacctgtttc tggcgggctt catcggagcg ttggccatct gcctgatagc
     7261 cgtgttcctg cagcgcgaca gcgtgttctt ctcgtaccgc gtctccatgc tgatgctgtg
     7321 cttgggatgc ttgtccacgc cgttcatggc cgacaacaac acgtattcga gcgcgcttat
     7381 cttcggcggc taccactgct tcgtgctggt gctgtgcgtg gtgtgcatcg acgtggcgtc
     7441 cagcttccgc gtgagcccgg cgcggacgat cggcctgggg ttcgtggcgc tgtacggcgg
     7501 cgagaccgca ggccagctgt tcgcccattc gctggaggcg accggggtca gcatgttcga
     7561 cctcacgctg gtcacgctgg tggccgtgtc gctgctgttc atcgcgcatt tgttcctgtt
     7621 cacggagacc gacctcatca aaatcggcat cggcgaggtc agcctgatgg gcacggtgcc
     7681 cgaagaggcc gccgacgacc cggccgaccc ggtggatccg tgcgcgctga tcgcggagcg
     7741 cttcgccctg tcgccgcgcg agaccgacgt gcttccgctg ctgctggagg gccgcaccat
     7801 ctcgcgcatc caggagacgc tgttcatctc ggcgggcacg gtgagcacgc atatccgcca
     7861 catctaccag aagacgggcg tggacaatcg ccaggagctc atcgacctgg cccacgagac
     7921 ggccgcgcag gacgaggcgg tctgaggcga tggagacggc gtatctgcac gagttcgcga
     7981 aggtggccga ttgcggcagc ttcacggccg ccgcgcgcga gctgcatctg acgcagtcga
     8041 cgctgagcaa gcacgtggcc ttgttggagc gcgaattcgg ggccgacctg ttcgtgcgcg
     8101 accgcagcgg cgtgagcctg accgaggcgg gcggcgtgct gtacgcccag gcgcggcaga
     8161 tggagaagct gctgcgcgcc accgagctgc tggtgcgcgc ggcgcgcgac gggcagggtt
     8221 cggcgggcgc gggggttgcg ggcgcgacgg cgggcgcagc ggcgggcggg ggtgccgtcg
     8281 acggctcgcc tgcggccgcg tgcgacacgg cgcttcgctg caagtgccgg ctcgcggccg
     8341 agcgctgcgg gctcgaccgg cgcgagctgg gcgcgctcat cctgtacgtg gaggagcgcg
     8401 gcttcgaggc gatccagggc gagctggcgc tgacgcgcga cgaggtggcc gacgtgctgg
     8461 gcagcgtgta ccgcaagctg ggcgtgggcg acaagcagga ggcgctcgat ttcatccatt
     8521 ccgtttcgga atgatggtag acgcgcccgc ggcggttggg catactgcgt agaagtgaaa
     8581 gcgctatgaa gctggggggc ttcgagcaaa gggcttcatc cgaagaatgg atggagaccg
     8641 aatatgagtg cttggactgc tgcgtccgtc gaagacgcgg cgaacaatct tcttgaagaa
     8701 ctgaatagcg gcgcgatgcg cttgtgcgtg gagcatgacg cgtccgacga cgttgcgccg
     8761 gcgttgctgc gcgcggctcg cgctcgcgtc gaggcggggt cgaacgtgcg cgtcgtgtgc
     8821 gccgacgccg ttgcagctgc gcgctttcgg gcgcttgcgg ccgacgatcc gctgctgggc
     8881 gcgctcgacg tggtatcggt gcgcgagctg gccctctcga ttctaggcga cgcgcgggtc
     8941 ggcgatgccg tgggacgcga cgcgcgcgtg ctggacgaga acgagctggc cgtgctcatg
     9001 gaggacgtga aggtgagcgg cctcaagccg ggccggctgc gcgagatgct caagttcttc
     9061 tacaagagca tcagcgactg cgccgacgat gacgagcgct ggctgatcac cgccgaagag
     9121 cagaccgtgc acgccatcct caccgagaac ctggaggcgc gccgcgcgtt gctgccgtgc
     9181 gaggtttcgt cgctggcgta tcgcggcctt gtgaaagccg gggtcgagcg cgagccgctg
     9241 acgctggtgg cagacgactt cgggtcgctc agcaaggcgt cgcagcgcct cgtgcgccat
     9301 ctggcgacgg acggcctcgt ggcggcgggc cgcacgctgg cctcgtccag ctccgaggag
     9361 ccgtatccgt gcttcgacgg gttccgcgcg cttgccgacg aggccgactg cctggtgacg
     9421 ctggcgtgcg agcggccggc cgcggcgcgc gagtcccgcg cgctggacga cccggcggcc
     9481 gagttcgcgt tcgcggcggc gtccgtggag gagtgcctgg ccgacggctt ccggccaggc
     9541 gacgtggctg tggccgtgcc gaacgccgct tggggcgagc gcatcgccgc cgagctggaa
     9601 gggcgcggca tcgccgtgga gcgcgggttc gacagcggga agatcaaggg cgacccgcgc
     9661 accgagggcc gttacgccga cctcaagctg gccgcgttcc tgcggttgta cctcgatccg
     9721 cacgatttca cgtcgttgcg ctcgtggctg gggttcggcg attggctgct gcgctccgat
     9781 gcgtttttgg agctgatggc gtacgcgcgc gaccatgaga cgaccgtgcc cgaagccgtg
     9841 gcgcagctgc gcacgcagcg cgacgccgat cgctcctcga cgatattcgg gaagttcgac
     9901 ggcccgctcg acgagctgga cgagctgctg cgcgcctgcg aggaagggct gacgcgcgag
     9961 gccgccgtag cgctgttcga agcgcacgga atgccgttgt ccccgcgcca cgtggagctg
    10021 ctgggcgacg accctgcgca tgcggacgtc gagcgcttgg cgcgctgcgg gttcgcgaag
    10081 ccggtggagg atgtcgcgcg cgatgccgtg catgtggtct cctacgcgcg ctgccacggg
    10141 caccatgtgc gcacggtgtt cgtcaccggc ctggtgaacg gcttcttgcc ggcgagcgac
    10201 gccgtggacg acaagttcac catcgaccat cggcgcgtgg cgctcgaacg cgagcgcgcg
    10261 ctgttcctgg acgtgctgtc ctgtgcgagc gaacgagcgg tatgcacgcg gttcgtgcgc
    10321 gaccttttag agaacaccgc gttcacgaag gtgcagacct cgcgcgtgtt cgtgcgcgac
    10381 ggcgagcgct tcgcgcgcgt gacgccgtcg gagttcgcgt cgctgaccga cgaggtgctc
    10441 tctcccgcgc ccgacgcgcc tcgcgagctg ccgacgaagg tgctgtacgc gtcttcgact
    10501 ttgtagctcc aacggtctga cgaccgcgcg aaccttcgct gacttttcct gaccggtgat
    10561 tttgaaatga tgaaaggact gatagatatg agcgatgttg atgtattcgt gatcgatgac
    10621 gatttcgatc ccctggccga cctgagcctg gacgacggcg agcgggacga tgccgaggcg
    10681 gattacctgc cgcccatccc cgatgccgac aagagcgtgg tgcctcccgt ggtgccgttg
    10741 tctgcgaccg agcgcatcga gaagctgctg gcgggcattc ccggccagca gttccgcctg
    10801 ctgcacgccg tggaattctg caccgagccg aagacgatgg acgaggccgt ggccaatttg
    10861 gacgcggcgt atccgaacac gacgagcgtg tacggctcgg cgcaggtggt gcagctgctc
    10921 gagcgcgacg gcgcgctgga gcgcatcgtg gacgaggacg ccgcccatgc ggccgccggc
    10981 gacgctgccg atacgcccgc cgaggacgag ggcttcatct cggtgacgcc cgctccgccg
    11041 tgtcactacc gtgccacgca agcgggcctc gatgccgtcg cggcgcacgt caacgagggc
    11101 ctcgtggcgg agcggatcag cgaagacgag cgctatctgc ccatcttcca gcgcgtgctt
    11161 gagatgtgcg cgcgcgaagg aggctgcccc accaaggagc tggaccaggc ggtggacggc
    11221 gacccgctgt gccaagagcc gcgccgcttc tgcgggttct tccgcggcaa gctggaagaa
    11281 acaggcgcca tcgaatggcg cgacgcctgg accattaccg atctggggcg cagcgtgctc
    11341 gcgtccggct tgttcgccgc cgcttccccg tccgaacgct agagaaggag ccacccatgg
    11401 acgagcacgg catcgagccc aagcgcgata cgggcccctg cgtgtcgcgc cgcacgttcg
    11461 ccctgggagc cgtgggagcg tgcgccgtca tcgggctggg cggcgtgaag tacctgccca
    11521 gtgccacgtt gctgcgcccg ccgggcgggc aggacgagga gcagctggcg cgcggctgcc
    11581 tgcattgcga gaagtgccgc gaggtgtgcc cgaagcatgc catcgctcct gctcatcttg
    11641 aggacggcat cttgaacgcg cgcacaccgc gcatggactt caaaagcggc tggtgcgatt
    11701 tctgcgagaa cgagccgggc gggccgaagt gcgtcgcggc gtgcccgacg catgcgcttt
    11761 cgtgccccga cccgtcgcag gcgatcatcg gcaaggcgga gatcaaccgc gactggtgcc
    11821 ttgccgcgaa gggcatgggc tgccacgagt gcgtcgacgt gtgcaactac gaggcgctcg
    11881 agctgggcgc cgacaacgtg ccggtggtgg acgtggatgc gtgcaacggc tgcggcgcct
    11941 gcgagctggc gtgcatctcg ctgtcggcag gttccatcac ggccggcgcc accgatcgcg
    12001 ccatcgtcgt ccgtccgacc gaagaagtgg aggcgtgatc aggatgaaga agctcaaaac
    12061 ctatcggctg cggttcctga tcatgctggc gctgctggcc gtggtggccg tggggtactt
    12121 cacggccggc ggcatcggca acttctgcgg catcggcttc gagtccatca cgctgctgtg
    12181 ccccctaggc gcgctgctgg ccatgatcgc cgagcgcaca gccatcccga tggccgtgat
    12241 cagcgttgcg gtcgtgctgc tggtgtgtat cgtgctaggc aaggtgttct gcgcctgggc
    12301 gtgccccgtg cacttcctgt cgcgaggaag gaagaagggg gataaggggg acaaggaggg
    12361 taaagggggc aaggttgctt ccccttgctc cgcgtgcgct tccccttgcg gcaaagccaa
    12421 gggcatcaag atcgacagcc gtcacggcat cctcgctgcg gcgttgggct ccacgctcgt
    12481 cttcggcttc ccggtgttct gcctcgtctg cccgatcggc ctcacgttcg ccacggtgct
    12541 gctggtcatg cgcctgttcg cgttcggcga cgccacgtgg acggtcgtcg ccttcccggc
    12601 catcatcgcg ctggagatcc tgctgctgcc gaagtggtgc cagcgcttct gcccgttggg
    12661 cgcgttgctt tcgctgttca gcgggctgaa ccggacgttc cgcccccagg tggacgccga
    12721 gaagtgcctg cgcgagggtc gcggcaaggc ctgcaacctg tgcgagcagg catgccccga
    12781 gggcatcaac ctgcacgatg tcgcggcggg cgagaccacg ctcaacgact gcagcaagtg
    12841 ccgcgcgtgc gccgacgtgt gccccgagcg cgccatcacg ttcccgttgc tgccgaagaa
    12901 gcaggcgcag gcgacgccgc tgcccgtcga cgacgagatc ccggaaaggg gataggccat
    12961 gtccctgatc aacggcctgc tggccctgtt gggcgagatc gagagcgatg ccatcgccgt
    13021 gcatgaggaa cgctgcatct cggttcgcaa ccgcaacgcc gactgcctgc gctgcgtgga
    13081 ggcatgcacg tcgggcgcgc tggcgtaccg cgcgggcgag ctgctcgtag agcccgagcg
    13141 ctgcatcgga tgcggcacgt gcgccacggc gtgccccacc tgcgccatcg agctgcgcaa
    13201 cccgacggat gccgagctga cggcgcagct caagcactcc atcgtggcga cgaaaggcca
    13261 tccggtcatc gtgtgcgagc aggcgctcga ggcggccaac gtcgcggccg acgatgcgtc
    13321 ggccgcgtgc gcggtgccgt gcctggggcg catggacgag tcggcgctcg tggggttggc
    13381 ggcgtaccgc gccttcgacg cgacgctggc ctgcggttcg tgcgagacgt gcccacatgc
    13441 tccgggcggc gcgctcgtgc gcgaggtggt ggagggcgcg cgcaacctgc ttgaggcgtt
    13501 cggatcctcc atgcccatcg acctggagga atgcgtgccc gagcgcgtgt tggagcccgg
    13561 cgggtcgcac ggccaggcag gcagcgacgg ggtggggcgg cgcgacttct tccgctcggc
    13621 caaggacgca tcgacccgcg cagctggcgc ggcggtggcc gaggagttgg ggatggtcga
    13681 ggccgagccc gttcctgcgg cgcatcgcaa agtgggcgca gatgggacgc tgtcgcagtt
    13741 cgtccccacg cgccgcgtgc ggctgtacaa ctacctgcag cacgtggggc aaccgacggc
    13801 cgacgaggtg gagacgcgtg tcatcggcgc cgtgtccatc gatgcccaga agtgcagttc
    13861 gtgccgcatg tgcgcggtgt tctgtcccac gggcgccatc aagaagctcg acgagggcgg
    13921 cgtgttcggc atcgtgcacc gtccgagcgc ctgcatgcag tgccgcctgt gcgagcacat
    13981 ctgtcccgag caggccatcg cggtgagcgg gcgcgtgccc atcaagcagt tcatgggcaa
    14041 ggaagccgtg tgcttcgcta tgaagcgtcc aacctggacc ccgaacaagc cctcatcgat
    14101 gtacgataag ctgcacgcga cgctcggcga acacttggag atgtgcatgt tctagactag
    14161 tcgactcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact agagagagaa
    14221 tataaaaagc cagattatta atccggcttt tttattattt aattcctgtt ataaaaaaag
    14281 gatcaatttt gaactctctc ccaaagttga tcccttaacg atccctgcag gagaaggcca
    14341 tcctgacgga tggccttttt gaagctttct agaatcgggg gcccccgggg ggattctaga
    14401 acaatggaag tcctcgtctc gccctcgaat tagcccgctt aatgagcggg ctttttttga
    14461 attaattctc gcgagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
    14521 cgcaattaat gtaagttagc gcgaattgca agctggccga cgcgctgggc tacgtcttgc
    14581 tggcgttcgg gagcagaaga gcatacatct ggaagcaaag ccaggaaagc ggcctatgga
    14641 gctgtgcggc agcgctcagt aggcaatttt tcaaaatatt gttaagcctt ttctgagcat
    14701 ggtatttttc atggtattac caattagcag gaaaataagc cattgaatat aaaagataaa
    14761 aatgtcttgt ttacaataga gtgggggggg tcagcctgcc gccttgggcc gggtgatgtc
    14821 gtacttgccc gccgcgaact cggttaccgt ccagcccagc gcgaccagct ccggcaacgc
    14881 ctcgcgcacc cgctggcggc gcttgcgcat ggtcgaacca ctggcctctg acggccagac
    14941 atagccgcac aaggtatcta tggaagcctt gccggttttg ccggggtcga tccagccaca
    15001 cagccgctgg tgcagcaggc gggcggtttc gctgtccagc gcccgcacct cgtccatgct
    15061 gatgcgcaca tgctggccgc cacccatgac ggcctgcgcg atcaaggggt tcagggccac
    15121 gtacaggcgc ccgtccgcct cgtcgctggc gtactccgac agcagccgaa acccctgccg
    15181 cttgcggcca ttctgggcga tgatggatac cttccaaagg cgctcgatgc agtcctgtat
    15241 gtgcttgagc gccccaccac tatcgacctc tgccccgatt tcctttgcca gcgcccgata
    15301 gctacctttg accaccatgg catcagcggt gacggcctcc cacttgggtt ccaggaacag
    15361 ccggagctgc cgtccgcctt cggtcttggg ttccgggcca agcactaggc cattaggccc
    15421 agccatggcc accagccctt gcaggatgcg cagatcatca gcgcccagcg gctccgggcc
    15481 gctgaactcg atccgcttgc cgtcgccgta gtcatacgtc acgtccagct tgctgcgctt
    15541 gcgctcgccc cgcttgaggg cacggaacag gccgggggcc agacagtgcg ccgggtcgtg
    15601 tcggacgtgg ctgaggctgt gcttgttctt aggcttcacc acggggcacc cccttgctct
    15661 tgcgctgcct ctccagcacg gcgggcttga gcaccccgcc gtcatgccgc ctgaaccacc
    15721 gatcagcgaa cggtgcgcca tagttggcct tgctcacacc gaagcggacg aagaaccggc
    15781 gctggtcgtc gtccacaccc cattcctcgg cctcggcgct ggtcatgctc gacaggtagg
    15841 actgccagcg gatgttatcg accagtaccg agctgccccg gctggcctgc tgctggtcgc
    15901 ctgcgcccat catggccgcg cccttgctgg catggtgcag gaacacgata gagcacccgg
    15961 tatcggcggc gatggcctcc atgcgaccga tgacctgggc catggggccg ctggcgtttt
    16021 cttcctcgat gtggaaccgg cgcagcgtgt ccagcaccat caggcggcgg ccctcggcgg
    16081 cgcgcttgag gccgtcgaac cactccgggg ccatgatgtt gggcaggctg ccgatcagcg
    16141 gctggatcag caggccgtca gccacggctt gccgttcctc ggcgctgagg tgcgccccaa
    16201 gggcgtgcag gcggtgatga atggcggtgg gcgggtcttc ggcgggcagg tagatcaccg
    16261 ggccggtggg cagttcgccc acctccagca gatccggccc gcctgcaatc tgtgcggcca
    16321 gttgcagggc cagcatggat ttaccggcac caccgggcga caccagcgcc ccgaccgtac
    16381 cggccaccat gttgggcaaa acgtagtcca gcggtggcgg cgctgctgcg aacgcctcca
    16441 gaatattgat aggcttatgg gtagccattg attgcctcct ttgcaggcag ttggtggtta
    16501 ggcgctggcg gggtcactac ccccgccctg cgccgctctg agttcttcca ggcactcgcg
    16561 cagcgcctcg tattcgtcgt cggtcagcca gaacttgcgc tgacgcatcc ctttggcctt
    16621 catgcgctcg gcatatcgcg cttggcgtac agcgtcaggg ctggccagca ggtcgccggt
    16681 ctgcttgtcc ttttggtctt tcatatcagt caccgagaaa cttgccgggg ccgaaaggct
    16741 tgtcttcgcg gaacaaggac aaggtgcagc cgtcaaggtt aaggctggcc atatcagcga
    16801 ctgaaaagcg gccagcctcg gccttgtttg acgtataacc aaagccaccg ggcaaccaat
    16861 agcccttgtc acttttgatc aggtagaccg accctgaagc gcttttttcg tattccataa
    16921 aacccccttc tgtgcgtgag tactcatagt ataacaggcg tgagtaccaa cgcaagcact
    16981 acatgctgaa atctggcccg cccctgtcca tgcctcgctg gcggggtgcc ggtgcccgtg
    17041 ccagctcggc ccgcgcaagc tggacgctgg gcagacccat gaccttgctg acggtgcgct
    17101 cgatgtaatc cgcttcgtgg ccgggcttgc gctctgccag cgctgggctg gcctcggcca
    17161 tggccttgcc gatttcctcg gcactgcggc cccggctggc cagcttctgc gcggcgataa
    17221 agtcgcactt gctgaggtca tcaccgaagc gcttgaccag cccggccatc tcgctgcggt
    17281 actcgtccag cgccgtgcgc cggtggcggc taagctgccg ctcggacagt tcgaggctgg
    17341 ccagcctgcg ggccttctcc tgctgccgct gggcctgctc gatctgctgg ccagcctgct
    17401 gcaccagcgc cgggccagcg gtggcggtct tgcccttgga ttcacgcagc agcacccacg
    17461 gctgataacc ggcgcgggtg gtgtgcttgt ccttgcggtt ggtgaagccc gccaagcggc
    17521 catagtggcg gctgtcggcg ctggccgggt cggcgtcgta ctcgctggcc agcgtccggg
    17581 caatctgccc ccgaagttca ccgcctgcgg cgtcggccac cttgacccat gcctgatagt
    17641 tcttcgggct ggtttccact accagggcag gctcccggcc ctcggctttc atgtcatcca
    17701 ggtcaaactc gctgaggtcg tccaccagca ccagaccatg ccgctcctgc tcggcgggcc
    17761 tgatatacac gtcattgccc tgggcattca tccgcttgag ccatggcgtg ttctggagca
    17821 cttcggcggc tgaccattcc cggttcatca tctggccggt ggtggcgtcc ctgacgccga
    17881 tatcgaagcg ctcacagccc atggccttga gctgtcggcc tatggcctgc aaagtcctgt
    17941 cgttcttcat cgggccacca agcgcagcca gatcgagccg tcctcggttg tcagtggcgt
    18001 caggtcgagc aagagcaacg atgcgatcag cagcaccacc gtaggcatca tggaagccag
    18061 catcacggtt agccatagct tccagtgcca cccccgcgac gcgctccggg cgctctgcgc
    18121 ggcgctgctc acctcggcgg ctacctcccg caactctttg gccagctcca cccatgccgc
    18181 ccctgtctgg cgctgggctt tcagccactc cgccgcctgc gcctcgctgg cctgctgggt
    18241 ctggctcatg acctgccggg cttcgtcggc cagtgtcgcc atgctctggg ccagcggttc
    18301 gatctgctcc gctaactcgt tgatgcctct ggatttcttc actctgtcga ttgcgttcat
    18361 ggtctattgc ctcccggtat tcctgtaagt cgatgatctg ggcgttggcg gtgtcgatgt
    18421 tcagggccac gtctgcccgg tcggtgcgga tgccccggcc ttccatctcc accacgttcg
    18481 gccccaggtg aacaccgggc aggcgctcga tgccctgcgc ctcaagtgtt ctgtggtcaa
    18541 tgcgggcgtc gtggcctgcc cgctctaatg cccggttggc atggtcggcc catgcctcgc
    18601 gggtctgctc aagccatgcc ttgggcttga gcgcttcggt cttctgtgcc ccgcccttct
    18661 ccggggtctt gccgttgtac cgcttgaacc actgagcggc gggccgctcg atgccgtcat
    18721 tgatccgctc ggagatcatc aggtggcagt gcgggttctc gccgccaccg gcatggatgg
    18781 ccagcgtata cggcaggcgc tcggcaccgg tcaggtgctg ggcgaactcg gacgccagcg
    18841 ccttctgctg gtcgagggtc agctcgaccg gcagggcaaa ttcgacctcc ttgaacagcc
    18901 gcccattggc gcgttcatac aggtcggcag catcccagta gtcggcgggc cgctcgacga
    18961 actccggcat gtgcccggat tcggcgtgca agacttcatc catgtcgcgg gcatacttgc
    19021 cttcgcgctg gatgaagtcg gccttggccc tggccgattg gccgcccgac ctgctgccgg
    19081 ttttcgccgt aaggtgataa atcgccatgc tgcctcgctg ttgcttttgc ttttcggctc
    19141 catgcaatgg ccctcggaga gcgcaccgcc cgaagggtgg ccgttaggcc agtttctcga
    19201 agagaaaccg gtaagtgcgc cctcccctac aaagtagggt cgggattgcc gccgctgtgc
    19261 ctccatgata gcctacgaga cagcacatta acaatggggt gtcaagatgg ttaaggggag
    19321 caacaaggcg gcggatcggc tggccaagct cgaagaacaa cgagcgcgaa tcaatgccga
    19381 aattcagcgg gtgcgggcaa gggaacagca gcaagagcgc aagaacgaaa caaggcgcaa
    19441 ggtgctggtg ggggccatga ttttggccaa ggtgaacagc agcgagtggc cggaggatcg
    19501 gctcatggcg gcaatggatg cgtaccttga acgcgaccac gaccgcgcct tgttcggtct
    19561 gccgccacgc cagaaggatg agccgggctg aatgatcgac cgagacaggc cctgcggggc
    19621 tgcacacgcg cccccaccct tcgggtaggg ggaaaggccg ctaaagcggc taaaagcgct
    19681 ccagcgtatt tctgcggggt ttggtgtggg gtttagcggg ctttgcccgc ctttccccct
    19741 gccgcgcagc ggtggggcgg tgtgtagcct agcgcagcga atagaccagc tatccggcct
    19801 ctggccgggc atattgggca agggcagcag cgccccacaa gggcgctgat aaccgcgcct
    19861 agtggattat tcttagataa tcatggatgg atttttccaa caccccgcca gcccccgccc
    19921 ctgctgggtt tgcaggtttg ggggcgtgac agttattgca ggggttcgtg acagttattg
    19981 caggggggcg tgacagttat tgcaggggtt cgtgacagtt agtacgggag tgacgggcac
    20041 tggctggcaa tgtctagcaa cggcaggcat ttcggctgag ggtaaaagaa ctttccgcta
    20101 agcgatagac tgtatgtaaa cacagtattg caaggacgcg gaacatgcct catgtggcgg
    20161 ccctcgcgag aattaattca gataaaaaaa atccttagct ttcgctaagg atgatttcta
    20221 gcgatgaccc tgctgattgg ttcgctgacc gtagactggt tggcttggtt tcatcagcca
    20281 tccgcttgcc ctcatctgtt acgccggcgg tagccggcca gcctcgcaga gcaggattcc
    20341 cgttgagcac cgccaggtgc gaataaggga cagtgaagaa ggaacacccg ctcgcgggtg
    20401 ggcctacttc acctatcctg cccggctgac gccgttggat acaccaagga aagtctacac
    20461 gaaccctttg gcaaaatcct gtatatcgtg cgacatcgcg cgcgcgcgcg cggatgctag
    20521 cggcgcgcca aaaaaaagcc cgctcatta
//
