LOCUS       sequence-463808-       20725 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Eggerthella lenta A2 Dadh (Delta-Elen_0474) in 
            Gordonibacter urolithinfaciens DSM 27213.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 20725)
  AUTHORS   Dong X, Bae M, Le C, Aguilar Ramos MA, Balskus EP
  TITLE     Enantiocomplementary Gut Bacterial Enzymes Metabolize Dietary 
            Polyphenols.
  JOURNAL   J Am Chem Soc. 2025 Mar 5;147(9):7231-7244. doi: 
            10.1021/jacs.4c09892. Epub 2025 Feb 24.
  PUBMED    39993729
REFERENCE   2  (bases 1 to 20725)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.4c09892"; journalName: "J Am Chem Soc"; date: 
            "2025-03-5- 5"; volume: "147"; issue: "9"; pages: "7231-7244"
COMMENT     Sequence Label: pXD80ENSF7.4-Dadh-Delta-Elen0474(ElA2)
FEATURES             Location/Qualifiers
     source          1..20725
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(19..813)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     primer_bind     1300..1318
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    1475..1494
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     CDS             complement(3005..3028)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     terminator      14349..14376
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      14508..14535
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(14967..15818)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(15805..16644)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(17155..18126)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELSERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     oriT            19365..19452
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     rep_origin      19794..20188
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     terminator      complement(20351..20394)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     oriT            20490..20599
                     /note="incP origin of transfer"
ORIGIN
        1 ggcgggctgg ccggccttct aaaacaattc atccagtaaa atataatatt ttattttctc
       61 ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg acatactgtt cttccccgat
      121 atcctccctg atcgaccgga cgcagaaggc aatgtcatac cacttgtccg ccctgccgct
      181 tctcccaaga tcaataaagc cacttacttt gccatctttc acaaagatgt tgctgtctcc
      241 caggtcgccg tgggaaaaga caagttcctc ttcgggcttt tccgtcttta aaaaatcata
      301 cagctcgcgc ggatctttaa atggagtgtc ttcttcccag ttttcgcaat ccacatcggc
      361 cagatcgtta ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc tattcgtata
      421 gggacaatcc gatatgtcga tggagtgaaa gagcctgatg cactccgcat acagctcgat
      481 aatcttttca gggctttgtt catcttcata ctcttccgag caaaggacgc catcggcctc
      541 actcatgagc agattgctcc agccatcatg ccgttcaaag tgcaggacct ttggaacagg
      601 cagctttcct tccagccata gcatcatgtc cttttcccgt tccacatcat aggtggtccc
      661 tttataccgg ctgtccgtca tttttaaata taggttttca ttttctccca ccagcttata
      721 taccttagca ggagacattc cttccgtatc ttttacgcag cggtattttt cgatcagttt
      781 tttcaattcc ggtgatattc tcattttagc catgatgttt aactttgttt tagggcgact
      841 gccctgctgc gtaacatcgt tgctgctcca taacatcaaa catcgaccca cggcgtaacg
      901 cgcttgctgc ttggatgccc gaggcataga ctgtacccca aaaaaacagt cataacaagc
      961 catgaaaacc gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt
     1021 gcgtgagcgc atacgctact tgcattacag cttacgaacc gaacaggctt atgtccactg
     1081 ggttcgtgcc ttcatccgtt tccacggtgt gcgtcacccg gcaaccttgg gcagcagcga
     1141 agtcgaggca tttctgtcct ggctggcgaa cgagcgcaag gtttcggtct ccacgcattc
     1201 gaaaccggta tcccgccgcc gccgccggat accggtttcg aattgagatt gccacctgac
     1261 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     1321 tttcgtcttc aaggcgaaaa aaccccgccg aagcggggtt ttttgcgaaa tataatgacc
     1381 ctcttgataa cccaagaggg cattttttag gaacggttat ttgattatta atccggcggc
     1441 aactgaaacg attcggatcc tgtattacta ttctgaattc gcggccgctt ctagatttag
     1501 tacatcagga ccactgggta gcccagctgg tagatcagca cgcgaaacat cacggaggtg
     1561 gccagcgcgc cgatggcggc gacgaccatg agcgtcttgg aggcgcccac cttcttcttc
     1621 gccacgaagg cgcaggccgc agccacgatc acggagacga tggagcccca gaacatgggc
     1681 gctcccgcgc cggacaccat gacggtcatc aggctgtcga cgtgcgtggg ggccgtggtc
     1741 atcgatgtcg ggtcggcgta gtagccgaat tgcgcgatct tcgccatggc gcaggcgatg
     1801 acgaacacca cgtcggccac gatctgcacg agggccgcga tgaacgtcag ctggatgccc
     1861 gtcgcctcga cggcctcgtc cttcttcagg atggagatca gccagacggc cgcggcgccc
     1921 aggaggcacg cgttgcccag atagaacgcc acgagcgcca ggccccacgc gggacgggcc
     1981 ggcatgaggt aggagtgcgc ggtggcgacc atcatgagca cggcaaccgc gagggtcgcc
     2041 caggccagcg ccttgggaac gggcttgccg cctcgaagga ccacgaacca tgcgacgatg
     2101 acgatggcca gcgccacgca tccgatgagc tcctgggtga tgcccgacgt gatgtgcccg
     2161 aagccgttga agatgcgctc ccagtgctcg aggtgcagga aggctccgat gccgcctacc
     2221 accagcgaga cggcggacgt gatgagcgcg gtcatctgca ggttcctgcc ctggcccttg
     2281 agcgccagga tggacatgcc gcccagcgtg ccgcaggtca agcatacgaa cagcgtgaag
     2341 atgacgagag gccactgcgt ttccattact catcacctcc ttcccagtga tggtcgcgta
     2401 ggatgtagga gtacgacggc ttgttgccca cgtcgggcag cttgtgcacg tcggcgtcgg
     2461 tgaacggctc caggaactcc accatcttgc ggcgcttgcc cagcgggtcc tcggagccct
     2521 cgaacgactc gtagccggcc tcggtgtcgc cgatccagcg ggcgttgccg ccgcactggc
     2581 tgacgcactg gggcagctcg ccctgcgaca gcttctgctc gcacagcgtg cacttctcga
     2641 cgaccttctc ctcctcgttg aggtagcgga cgccgtaggg gcaggccatg acgcagaact
     2701 ggcagccgat gcacttctcc ttgtcgatct ggatggagcc gtcctcggcc acgtgcgacg
     2761 cctcggtggg gcacaccttc acgcactcgg ggttctcgca gtgctggcag ctgatgggca
     2821 ggaagtacat gtacacgtcg gggtactgcc ctgatccgcc cgggatgggg ttcggcccca
     2881 cgcgcaccac tttgttccag tagctgccga tgggcacgtt gttgaccgcc ttgcaggcga
     2941 ccgagcacgc caggcagccc acgcaccggt tgaggtcggt gatgatagcc ttcgtcatgg
     3001 ttcagtgatg gtgatggtga tggtgatgct cgagtgcggc ctccctccct tcgtaggtag
     3061 gcagccattc cttcagacgg gggtcgtcgg aggtatggat gatgggcgtg ccggtggagt
     3121 cgcacggcac ggggttgttg aacggcgagt tctcgggcgt ggccttgtag atcttcacct
     3181 ggtaggcgcg caggttggac gtgccggagt gcgggtcctg ggcgtagtgg tcgatcagct
     3241 ggttcacctg ggagaactgc cagccgtggc cggcgtcctt cacctcgggg taccaccacg
     3301 tgtgctcgag gttgatgacg tcttccttca cgccgtagta caggtcagcc acttcgcgga
     3361 tcttgcccca ctcggtttcg atccacaccc agtcgccctg ctcgatgccg tactcggcgg
     3421 ccgtcttcgg gttgatctcc acgcgcggca cgggccacag ctcgcggcac cagggaagct
     3481 ggcgatgctc ggagtggaag tacaccggga tgcgacggcc ggtggtggcg gtcagcgggt
     3541 actccttgat gcggtcgccg tccttcgggc cgtgaggcgg ctcggtgtag gtgggaagcc
     3601 tccactcggg atggtcggga tggtgcgatt ccatgacggt ggaccagatc tcctgcttca
     3661 tcgtcggggt gaaccagccc ggcttccagt cgccgatgcc cttgccggcc gtgtagtcga
     3721 ggcggcccca cacgcggtcg cgtgcgcgca tcgcgccggt ctggtagcgg cggtacgtgc
     3781 cccagttgcg cggctcgatg tccttcgcct gccaccagcc gtgctcctgg aaggccttga
     3841 cgtactcgtc ccacgtcttg tacttgggcg tgacctcggc catcggaacg ccgtggcgct
     3901 cgaacgtggg cttgccgttc tcgacgtgcc acgtggtgta ggtcagctcg tcgtcggtga
     3961 gcagcttgat ggagtcgtcc agctgccagt tgatgtcggg ccactcgttg cccggctcgt
     4021 cggtccactt ccagttcatg cggcgggcga ggtccatgac gatctcggga tcgtacttgg
     4081 cttccgcggg cggctgcacg cacttgaccg tggcgcccat ggcgccggcg gaaccttggc
     4141 tggcgcgcgg cgagttgagc tcgatccaat gggccaccgg caggatgatg tcggcggtgt
     4201 cgttctgcgg cgtgtgccac aggttgaggt ccacgtagaa gtcaagcgag cacagggcct
     4261 cccaggcgcg cgtggtgttc gtctggctca tgaagttgcc ggactcgttc cagagggcgc
     4321 gcaccgggta ggggtcgccc gtgatgacgg cgtcccacag cgaatggctg tcgcaccagt
     4381 actgccacca gcccagaagc gggaacttgt cgatgccgat ctgcttgcgg ttgacttcct
     4441 ccggcggggt ggtggcgccg ggggcccagg cgctgaagcc ctggaggtcg ccgtcgatgg
     4501 ggacgatggt cgggccgcgg ttgccgcccg gggtgtccat gttgccggtg atgcccacga
     4561 ggttgtcgaa ggcgcggcag ttctggatgg cgctgcagaa gtgctcgacc gccagcatgt
     4621 actggatgcc gccgttgccg tagccggtgg acgggtcgat gcgcgtgccg taggccgtgg
     4681 ccgccgcctc gatctcggag gcggggatgc cggtgatctc ggccgccacc tcgggcttgt
     4741 actcggcggc gcgggccttg tagtgctccc acaccggctt gaccttcacg gtcttgccgt
     4801 ccttcagcgt gatctcgaac tcgccttcca gcgcggggtc gatggcgggg tcgaacggca
     4861 tgggatcggg cagccagccc tgcgtctggc ccggcagcag gttgtcctgc gcggcctcgc
     4921 ggccttggcg cgccttctcg gagtcgtagt tctcgccctc ccagaagccg ccggtctcgt
     4981 cgatgacgcc ttcctggtcg gcgttgaacc aggtgaacgc gccgtactcg tgctcgacgc
     5041 cctcggcctt cagcttctcc cagttgttgt cgtacacgag gaacttatag gggctgccgc
     5101 cctccttgat gtcgctttcc ttgagcagcg cggttttcac gtcccagtag gaaccgtcgg
     5161 tgcgcacggt ggggaagccg gagggctcca tgtcctcgca cacgaggaac ggggcgttcg
     5221 tccacttctt cacgtacagc tcgtcgtaga gcttcttctc gatgatgacg ttcgtccagg
     5281 ccagggccag ggcgccgtcg gtgccggggc gcaggtgctg ccagtagtcg gcttccttgc
     5341 ccatgttggc catgcggggg tcgacgctga tgtgcacgtc ggcgcgcgat gccacgtcca
     5401 ccgtggtgcg gcaggagtcg tcgtagttgg acagctcgga cgcgccgccc cactgcacgt
     5461 acactttcgg gcgcgtgatg gtttccatcc acgacatggc gaactgcgac accatggtgg
     5521 tggcgaagtg gcgcgggccc ttgcagatct gccaggcctc aacgttgttc ggcgtttcca
     5581 gcatgttctt caggatggac tccgagtgca tgcaccagat gcgcgacgtg cccacctggc
     5641 aggcgatggc ctcgccgccg tgcttcgcct tgatgtccat gaagttgtcc acgatggact
     5701 gcatggcctc gtcccacgag atgcgctgcc agccgggctc ctcgcccttc gggttcgtgc
     5761 gcttcatggg atagtgcaga cggtcggggt ggtacgccgc ctggatggac gactgcgact
     5821 tcgtgcagca gttgcccatg gactggaacg ctccctcgtc gccctcgacg cgtatggccc
     5881 gcccgttctg gacgatgacc ttgacgccgc actccatctt gccgcagccg cggcagcacg
     5941 ttttcacggc cacggtgtcg ttgccggaca cgctgctgta cgtctcttcg gccagcgcgg
     6001 tgtgcgtcga agcgccgaac gccgcggctg ccgcaccggt gatggcggcg gtcttgacga
     6061 acgtgcgtcg tgacatggtc aggttaccca tactcctcct ccttctttct cctcgttctc
     6121 gtcttgctcg tcttcgttcg tgcgatcggg gaggggggct gccgggtgaa gcctcgggct
     6181 ccgaaccggt gcaaagtccc cgtgtcgctt gggccgacca tacggatgcg agggggtgca
     6241 gagcatcgtc caaaggttgg aaagcaggtt cgaccgcatc ataaattctt atgatcttgt
     6301 gatcatgaaa aattcgatag tgctttgaac tggggaaact ttaaccgtag gtcataaatt
     6361 attctgaaac tagaatagtt tcctcaaaga gggccgctca tcttttccgt ataatcgaac
     6421 ccgtcgacag aggggtcggc gcgagcgacg ttatgtacgg gctcacaggg ggtggggcat
     6481 ggaaaagctt cggagtgcat gggataccgc gttggggacg agcctttccc tcaagcgcat
     6541 cgcgggcgcg gccttgcaat ggctttggat gctcatgatg ttctacagca tcgtgccgta
     6601 cggcttctcg tcggacgtgc gcggctcgct gtacgtcagc ctgatcatat cgctggtggc
     6661 catggtggtg accatgctgt tcgtctccgt catcgtgcgg cgcgagcggg tgagcggcaa
     6721 ccgctccatc gtgctgggat cggccatcat gatgagcgcg ggcagcgtgc tcacgccgtt
     6781 ctccgatcct accacgcccg tcggcatgct ggtgctgggc ctgagcgcgg tcatgaccgg
     6841 cacgggctcg gccgtgctgt tcttgtgctg gatcgagctc gagtcggggc tgggaggccg
     6901 cttggcgctt gtggagcttg cctcgtcgct gtgcatcgcg ttcgtcgtgg ggttcttgct
     6961 gatcgtaggg ccggcgatac ccgtcatcgt gctcatcgtt gtcatgcccg tgctttcggc
     7021 ggcagctctc aggcgcagcg cgcccgatac gccgcagctg ccgcgcgagc ccgagcagcc
     7081 tttgtcgcgc catacggcga ccctgttcgc gaaggcgctt ctgggcgcgg tgctcatcgg
     7141 catgttgatg gggttcttcg acgtggtgtc ggggttcaaa acctacgagg tgcaggacat
     7201 cttcggcacg tacctgtttc tggcgggctt catcggagcg ttggccatct gcctgatagc
     7261 cgtgttcctg cagcgcgaca gcgtgttctt ctcgtaccgc gtctccatgc tgatgctgtg
     7321 cttgggatgc ttgtccacgc cgttcatggc cgacaacaac acgtattcga gcgcgcttat
     7381 cttcggcggc taccactgct tcgtgctggt gctgtgcgtg gtgtgcatcg acgtggcgtc
     7441 cagcttccgc gtgagcccgg cgcggacgat cggcctgggg ttcgtggcgc tgtacggcgg
     7501 cgagaccgca ggccagctgt tcgcccattc gctggaggcg accggggtca gcatgttcga
     7561 cctcacgctg gtcacgctgg tggccgtgtc gctgctgttc atcgcgcatt tgttcctgtt
     7621 cacggagacc gacctcatca aaatcggcat cggcgaggtc agcctgatgg gcacggtgcc
     7681 cgaagaggcc gccgacgacc cggccgaccc ggtggatccg tgcgcgctga tcgcggagcg
     7741 cttcgccctg tcgccgcgcg agaccgacgt gcttccgctg ctgctggagg gccgcaccat
     7801 ctcgcgcatc caggagacgc tgttcatctc ggcgggcacg gtgagcacgc atatccgcca
     7861 catctaccag aagacgggcg tggacaatcg ccaggagctc atcgacctgg cccacgagac
     7921 ggccgcgcag gacgaggcgg tctgaggcgt ggtagacgcg cccgcggcgg ttgggcatac
     7981 tgcgtagaag tgaaagcgct atgaagctgg ggggcttcga gcaaagggct tcatccgaag
     8041 aatggatgga gaccgaatat gagtgcttgg actgctgcgt ccgtcgaaga cgcggcgaac
     8101 aatcttcttg aagaactgaa tagcggcgcg atgcgcttgt gcgtggagca tgacgcgtcc
     8161 gacgacgttg cgccggcgtt gctgcgcgcg gctcgcgctc gcgtcgaggc ggggtcgaac
     8221 gtgcgcgtcg tgtgcgccga cgccgttgca gctgcgcgct ttcgggcgct tgcggccgac
     8281 gatccgctgc tgggcgcgct cgacgtggta tcggtgcgcg agctggccct ctcgattcta
     8341 ggcgacgcgc gggtcggcga tgccgtggga cgcgacgcgc gcgtgctgga cgagaacgag
     8401 ctggccgtgc tcatggagga cgtgaaggtg agcggcctca agccgggccg gctgcgcgag
     8461 atgctcaagt tcttctacaa gagcatcagc gactgcgccg acgatgacga gcgctggctg
     8521 atcaccgccg aagagcagac cgtgcacgcc atcctcaccg agaacctgga ggcgcgccgc
     8581 gcgttgctgc cgtgcgaggt ttcgtcgctg gcgtatcgcg gccttgtgaa agccggggtc
     8641 gagcgcgagc cgctgacgct ggtggcagac gacttcgggt cgctcagcaa ggcgtcgcag
     8701 cgcctcgtgc gccatctggc gacggacggc ctcgtggcgg cgggccgcac gctggcctcg
     8761 tccagctccg aggagccgta tccgtgcttc gacgggttcc gcgcgcttgc cgacgaggcc
     8821 gactgcctgg tgacgctggc gtgcgagcgg ccggccgcgg cgcgcgagtc ccgcgcgctg
     8881 gacgacccgg cggccgagtt cgcgttcgcg gcggcgtccg tggaggagtg cctggccgac
     8941 ggcttccggc caggcgacgt ggctgtggcc gtgccgaacg ccgcttgggg cgagcgcatc
     9001 gccgccgagc tggaagggcg cggcatcgcc gtggagcgcg ggttcgacag cgggaagatc
     9061 aagggcgacc cgcgcaccga gggccgttac gccgacctca agctggccgc gttcctgcgg
     9121 ttgtacctcg atccgcacga tttcacgtcg ttgcgctcgt ggctggggtt cggcgattgg
     9181 ctgctgcgct ccgatgcgtt tttggagctg atggcgtacg cgcgcgacca tgagacgacc
     9241 gtgcccgaag ccgtggcgca gctgcgcacg cagcgcgacg ccgatcgctc ctcgacgata
     9301 ttcgggaagt tcgacggccc gctcgacgag ctggacgagc tgctgcgcgc ctgcgaggaa
     9361 gggctgacgc gcgaggccgc cgtagcgctg ttcgaagcgc acggaatgcc gttgtccccg
     9421 cgccacgtgg agctgctggg cgacgaccct gcgcatgcgg acgtcgagcg cttggcgcgc
     9481 tgcgggttcg cgaagccggt ggaggatgtc gcgcgcgatg ccgtgcatgt ggtctcctac
     9541 gcgcgctgcc acgggcacca tgtgcgcacg gtgttcgtca ccggcctggt gaacggcttc
     9601 ttgccggcga gcgacgccgt ggacgacaag ttcaccatcg accatcggcg cgtggcgctc
     9661 gaacgcgagc gcgcgctgtt cctggacgtg ctgtcctgtg cgagcgaacg agcggtatgc
     9721 acgcggttcg tgcgcgacct tttagagaac accgcgttca cgaaggtgca gacctcgcgc
     9781 gtgttcgtgc gcgacggcga gcgcttcgcg cgcgtgacgc cgtcggagtt cgcgtcgctg
     9841 accgacgagg tgctctctcc cgcgcccgac gcgcctcgcg agctgccgac gaaggtgctg
     9901 tacgcgtctt cgactttgta gctccaacgg tctgacgacc gcgcgaacct tcgctgactt
     9961 ttcctgaccg gtgattttga aatgatgaaa ggactgatag atatgagcga tgttgatgta
    10021 ttcgtgatcg atgacgattt cgatcccctg gccgacctga gcctggacga cggcgagcgg
    10081 gacgatgccg aggcggatta cctgccgccc atccccgatg ccgacaagag cgtggtgcct
    10141 cccgtggtgc cgttgtctgc gaccgagcgc atcgagaagc tgctggcggg cattcccggc
    10201 cagcagttcc gcctgctgca cgccgtggaa ttctgcaccg agccgaagac gatggacgag
    10261 gccgtggcca atttggacgc ggcgtatccg aacacgacga gcgtgtacgg ctcggcgcag
    10321 gtggtgcagc tgctcgagcg cgacggcgcg ctggagcgca tcgtggacga ggacgccgcc
    10381 catgcggccg ccggcgacgc tgccgatacg cccgccgagg acgagggctt catctcggtg
    10441 acgcccgctc cgccgtgtca ctaccgtgcc acgcaagcgg gcctcgatgc cgtcgcggcg
    10501 cacgtcaacg agggcctcgt ggcggagcgg atcagcgaag acgagcgcta tctgcccatc
    10561 ttccagcgcg tgcttgagat gtgcgcgcgc gaaggaggct gccccaccaa ggagctggac
    10621 caggcggtgg acggcgaccc gctgtgccaa gagccgcgcc gcttctgcgg gttcttccgc
    10681 ggcaagctgg aagaaacagg cgccatcgaa tggcgcgacg cctggaccat taccgatctg
    10741 gggcgcagcg tgctcgcgtc cggcttgttc gccgccgctt ccccgtccga acgctagaga
    10801 aggagccacc catgtcccac gatcaggaaa ccaccatcgc cgacgtgctg cgcggccgca
    10861 tggcaaccta ccagttcctc tcgcgcctgt tccgcgtgga ggtggaccag gagctgtacg
    10921 acacgctgat gtccatgcgc ttcccggcga acaccggcaa cgcgctggtg gacgaaggct
    10981 accggatgat ctgcggttat ctgagccaag ccgacggcac ggtgctcacc gagctggcgg
    11041 tggactacgt gcgcgcgttc atcgggtcgg gcaacgacgg cttctcggcc gcgtacccgt
    11101 acgagtcggt gtacacgagc ccgaagcgcc tcatgatgca ggacgcccgc gacgaggtgc
    11161 tggtgctgta ccgcgccttc ggtctggaca agcaggaaag ctggaaggag ggcgaggacc
    11221 atatcgcgct ggagctggag ttcgagcaga tcctatgcga gcgcgctatc agggcgtacg
    11281 aggccggcga cgaggacgag tgcctgaagc tgctgctgtc gcagcgcaac ttcctggagg
    11341 accacctgct ggcgtggtac cccatgatgg cggccgactt gcagaagttc ccgcagaccg
    11401 acttctacaa gggcctgggc aagctgaccg acggcttcct gcgcaacgac cgcgaatttt
    11461 tggacgccgt gctgtctgag aacgaagcgg attgccatcc agagcggagg ccgcaggccg
    11521 aagccgaagg atcccgtgcg gcgtcggccg aaaccgaagt cgaggttgca tgatggacga
    11581 gcacggcatc gagcccaagc gcgatacggg cccctgcgtg tcgcgccgca cgttcgccct
    11641 gggagccgtg ggagcgtgcg ccgtcatcgg gctgggcggc gtgaagtacc tgcccagtgc
    11701 cacgttgctg cgcccgccgg gcgggcagga cgaggagcag ctggcgcgcg gctgcctgca
    11761 ttgcgagaag tgccgcgagg tgtgcccgaa gcatgccatc gctcctgctc atcttgagga
    11821 cggcatcttg aacgcgcgca caccgcgcat ggacttcaaa agcggctggt gcgatttctg
    11881 cgagaacgag ccgggcgggc cgaagtgcgt cgcggcgtgc ccgacgcatg cgctttcgtg
    11941 ccccgacccg tcgcaggcga tcatcggcaa ggcggagatc aaccgcgact ggtgccttgc
    12001 cgcgaagggc atgggctgcc acgagtgcgt cgacgtgtgc aactacgagg cgctcgagct
    12061 gggcgccgac aacgtgccgg tggtggacgt ggatgcgtgc aacggctgcg gcgcctgcga
    12121 gctggcgtgc atctcgctgt cggcaggttc catcacggcc ggcgccaccg atcgcgccat
    12181 cgtcgtccgt ccgaccgaag aagtggaggc gtgatcagga tgaagaagct caaaacctat
    12241 cggctgcggt tcctgatcat gctggcgctg ctggccgtgg tggccgtggg gtacttcacg
    12301 gccggcggca tcggcaactt ctgcggcatc ggcttcgagt ccatcacgct gctgtgcccc
    12361 ctaggcgcgc tgctggccat gatcgccgag cgcacagcca tcccgatggc cgtgatcagc
    12421 gttgcggtcg tgctgctggt gtgtatcgtg ctaggcaagg tgttctgcgc ctgggcgtgc
    12481 cccgtgcact tcctgtcgcg aggaaggaag aagggggata agggggacaa ggagggtaaa
    12541 gggggcaagg ttgcttcccc ttgctccgcg tgcgcttccc cttgcggcaa agccaagggc
    12601 atcaagatcg acagccgtca cggcatcctc gctgcggcgt tgggctccac gctcgtcttc
    12661 ggcttcccgg tgttctgcct cgtctgcccg atcggcctca cgttcgccac ggtgctgctg
    12721 gtcatgcgcc tgttcgcgtt cggcgacgcc acgtggacgg tcgtcgcctt cccggccatc
    12781 atcgcgctgg agatcctgct gctgccgaag tggtgccagc gcttctgccc gttgggcgcg
    12841 ttgctttcgc tgttcagcgg gctgaaccgg acgttccgcc cccaggtgga cgccgagaag
    12901 tgcctgcgcg agggtcgcgg caaggcctgc aacctgtgcg agcaggcatg ccccgagggc
    12961 atcaacctgc acgatgtcgc ggcgggcgag accacgctca acgactgcag caagtgccgc
    13021 gcgtgcgccg acgtgtgccc cgagcgcgcc atcacgttcc cgttgctgcc gaagaagcag
    13081 gcgcaggcga cgccgctgcc cgtcgacgac gagatcccgg aaaggggata ggccatgtcc
    13141 ctgatcaacg gcctgctggc cctgttgggc gagatcgaga gcgatgccat cgccgtgcat
    13201 gaggaacgct gcatctcggt tcgcaaccgc aacgccgact gcctgcgctg cgtggaggca
    13261 tgcacgtcgg gcgcgctggc gtaccgcgcg ggcgagctgc tcgtagagcc cgagcgctgc
    13321 atcggatgcg gcacgtgcgc cacggcgtgc cccacctgcg ccatcgagct gcgcaacccg
    13381 acggatgccg agctgacggc gcagctcaag cactccatcg tggcgacgaa aggccatccg
    13441 gtcatcgtgt gcgagcaggc gctcgaggcg gccaacgtcg cggccgacga tgcgtcggcc
    13501 gcgtgcgcgg tgccgtgcct ggggcgcatg gacgagtcgg cgctcgtggg gttggcggcg
    13561 taccgcgcct tcgacgcgac gctggcctgc ggttcgtgcg agacgtgccc acatgctccg
    13621 ggcggcgcgc tcgtgcgcga ggtggtggag ggcgcgcgca acctgcttga ggcgttcgga
    13681 tcctccatgc ccatcgacct ggaggaatgc gtgcccgagc gcgtgttgga gcccggcggg
    13741 tcgcacggcc aggcaggcag cgacggggtg gggcggcgcg acttcttccg ctcggccaag
    13801 gacgcatcga cccgcgcagc tggcgcggcg gtggccgagg agttggggat ggtcgaggcc
    13861 gagcccgttc ctgcggcgca tcgcaaagtg ggcgcagatg ggacgctgtc gcagttcgtc
    13921 cccacgcgcc gcgtgcggct gtacaactac ctgcagcacg tggggcaacc gacggccgac
    13981 gaggtggaga cgcgtgtcat cggcgccgtg tccatcgatg cccagaagtg cagttcgtgc
    14041 cgcatgtgcg cggtgttctg tcccacgggc gccatcaaga agctcgacga gggcggcgtg
    14101 ttcggcatcg tgcaccgtcc gagcgcctgc atgcagtgcc gcctgtgcga gcacatctgt
    14161 cccgagcagg ccatcgcggt gagcgggcgc gtgcccatca agcagttcat gggcaaggaa
    14221 gccgtgtgct tcgctatgaa gcgtccaacc tggaccccga acaagccctc atcgatgtac
    14281 gataagctgc acgcgacgct cggcgaacac ttggagatgt gcatgttcta gactagtcga
    14341 ctcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatactagag agagaatata
    14401 aaaagccaga ttattaatcc ggctttttta ttatttaatt cctgttataa aaaaaggatc
    14461 aattttgaac tctctcccaa agttgatccc ttaacgatcc ctgcaggaga aggccatcct
    14521 gacggatggc ctttttgaag ctttctagaa tcgggggccc ccggggggat tctagaacaa
    14581 tggaagtcct cgtctcgccc tcgaattagc ccgcttaatg agcgggcttt ttttgaatta
    14641 attctcgcga gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
    14701 attaatgtaa gttagcgcga attgcaagct ggccgacgcg ctgggctacg tcttgctggc
    14761 gttcgggagc agaagagcat acatctggaa gcaaagccag gaaagcggcc tatggagctg
    14821 tgcggcagcg ctcagtaggc aatttttcaa aatattgtta agccttttct gagcatggta
    14881 tttttcatgg tattaccaat tagcaggaaa ataagccatt gaatataaaa gataaaaatg
    14941 tcttgtttac aatagagtgg ggggggtcag cctgccgcct tgggccgggt gatgtcgtac
    15001 ttgcccgccg cgaactcggt taccgtccag cccagcgcga ccagctccgg caacgcctcg
    15061 cgcacccgct ggcggcgctt gcgcatggtc gaaccactgg cctctgacgg ccagacatag
    15121 ccgcacaagg tatctatgga agccttgccg gttttgccgg ggtcgatcca gccacacagc
    15181 cgctggtgca gcaggcgggc ggtttcgctg tccagcgccc gcacctcgtc catgctgatg
    15241 cgcacatgct ggccgccacc catgacggcc tgcgcgatca aggggttcag ggccacgtac
    15301 aggcgcccgt ccgcctcgtc gctggcgtac tccgacagca gccgaaaccc ctgccgcttg
    15361 cggccattct gggcgatgat ggataccttc caaaggcgct cgatgcagtc ctgtatgtgc
    15421 ttgagcgccc caccactatc gacctctgcc ccgatttcct ttgccagcgc ccgatagcta
    15481 cctttgacca ccatggcatc agcggtgacg gcctcccact tgggttccag gaacagccgg
    15541 agctgccgtc cgccttcggt cttgggttcc gggccaagca ctaggccatt aggcccagcc
    15601 atggccacca gcccttgcag gatgcgcaga tcatcagcgc ccagcggctc cgggccgctg
    15661 aactcgatcc gcttgccgtc gccgtagtca tacgtcacgt ccagcttgct gcgcttgcgc
    15721 tcgccccgct tgagggcacg gaacaggccg ggggccagac agtgcgccgg gtcgtgtcgg
    15781 acgtggctga ggctgtgctt gttcttaggc ttcaccacgg ggcaccccct tgctcttgcg
    15841 ctgcctctcc agcacggcgg gcttgagcac cccgccgtca tgccgcctga accaccgatc
    15901 agcgaacggt gcgccatagt tggccttgct cacaccgaag cggacgaaga accggcgctg
    15961 gtcgtcgtcc acaccccatt cctcggcctc ggcgctggtc atgctcgaca ggtaggactg
    16021 ccagcggatg ttatcgacca gtaccgagct gccccggctg gcctgctgct ggtcgcctgc
    16081 gcccatcatg gccgcgccct tgctggcatg gtgcaggaac acgatagagc acccggtatc
    16141 ggcggcgatg gcctccatgc gaccgatgac ctgggccatg gggccgctgg cgttttcttc
    16201 ctcgatgtgg aaccggcgca gcgtgtccag caccatcagg cggcggccct cggcggcgcg
    16261 cttgaggccg tcgaaccact ccggggccat gatgttgggc aggctgccga tcagcggctg
    16321 gatcagcagg ccgtcagcca cggcttgccg ttcctcggcg ctgaggtgcg ccccaagggc
    16381 gtgcaggcgg tgatgaatgg cggtgggcgg gtcttcggcg ggcaggtaga tcaccgggcc
    16441 ggtgggcagt tcgcccacct ccagcagatc cggcccgcct gcaatctgtg cggccagttg
    16501 cagggccagc atggatttac cggcaccacc gggcgacacc agcgccccga ccgtaccggc
    16561 caccatgttg ggcaaaacgt agtccagcgg tggcggcgct gctgcgaacg cctccagaat
    16621 attgataggc ttatgggtag ccattgattg cctcctttgc aggcagttgg tggttaggcg
    16681 ctggcggggt cactaccccc gccctgcgcc gctctgagtt cttccaggca ctcgcgcagc
    16741 gcctcgtatt cgtcgtcggt cagccagaac ttgcgctgac gcatcccttt ggccttcatg
    16801 cgctcggcat atcgcgcttg gcgtacagcg tcagggctgg ccagcaggtc gccggtctgc
    16861 ttgtcctttt ggtctttcat atcagtcacc gagaaacttg ccggggccga aaggcttgtc
    16921 ttcgcggaac aaggacaagg tgcagccgtc aaggttaagg ctggccatat cagcgactga
    16981 aaagcggcca gcctcggcct tgtttgacgt ataaccaaag ccaccgggca accaatagcc
    17041 cttgtcactt ttgatcaggt agaccgaccc tgaagcgctt ttttcgtatt ccataaaacc
    17101 cccttctgtg cgtgagtact catagtataa caggcgtgag taccaacgca agcactacat
    17161 gctgaaatct ggcccgcccc tgtccatgcc tcgctggcgg ggtgccggtg cccgtgccag
    17221 ctcggcccgc gcaagctgga cgctgggcag acccatgacc ttgctgacgg tgcgctcgat
    17281 gtaatccgct tcgtggccgg gcttgcgctc tgccagcgct gggctggcct cggccatggc
    17341 cttgccgatt tcctcggcac tgcggccccg gctggccagc ttctgcgcgg cgataaagtc
    17401 gcacttgctg aggtcatcac cgaagcgctt gaccagcccg gccatctcgc tgcggtactc
    17461 gtccagcgcc gtgcgccggt ggcggctaag ctgccgctcg gacagttcga ggctggccag
    17521 cctgcgggcc ttctcctgct gccgctgggc ctgctcgatc tgctggccag cctgctgcac
    17581 cagcgccggg ccagcggtgg cggtcttgcc cttggattca cgcagcagca cccacggctg
    17641 ataaccggcg cgggtggtgt gcttgtcctt gcggttggtg aagcccgcca agcggccata
    17701 gtggcggctg tcggcgctgg ccgggtcggc gtcgtactcg ctggccagcg tccgggcaat
    17761 ctgcccccga agttcaccgc ctgcggcgtc ggccaccttg acccatgcct gatagttctt
    17821 cgggctggtt tccactacca gggcaggctc ccggccctcg gctttcatgt catccaggtc
    17881 aaactcgctg aggtcgtcca ccagcaccag accatgccgc tcctgctcgg cgggcctgat
    17941 atacacgtca ttgccctggg cattcatccg cttgagccat ggcgtgttct ggagcacttc
    18001 ggcggctgac cattcccggt tcatcatctg gccggtggtg gcgtccctga cgccgatatc
    18061 gaagcgctca cagcccatgg ccttgagctg tcggcctatg gcctgcaaag tcctgtcgtt
    18121 cttcatcggg ccaccaagcg cagccagatc gagccgtcct cggttgtcag tggcgtcagg
    18181 tcgagcaaga gcaacgatgc gatcagcagc accaccgtag gcatcatgga agccagcatc
    18241 acggttagcc atagcttcca gtgccacccc cgcgacgcgc tccgggcgct ctgcgcggcg
    18301 ctgctcacct cggcggctac ctcccgcaac tctttggcca gctccaccca tgccgcccct
    18361 gtctggcgct gggctttcag ccactccgcc gcctgcgcct cgctggcctg ctgggtctgg
    18421 ctcatgacct gccgggcttc gtcggccagt gtcgccatgc tctgggccag cggttcgatc
    18481 tgctccgcta actcgttgat gcctctggat ttcttcactc tgtcgattgc gttcatggtc
    18541 tattgcctcc cggtattcct gtaagtcgat gatctgggcg ttggcggtgt cgatgttcag
    18601 ggccacgtct gcccggtcgg tgcggatgcc ccggccttcc atctccacca cgttcggccc
    18661 caggtgaaca ccgggcaggc gctcgatgcc ctgcgcctca agtgttctgt ggtcaatgcg
    18721 ggcgtcgtgg cctgcccgct ctaatgcccg gttggcatgg tcggcccatg cctcgcgggt
    18781 ctgctcaagc catgccttgg gcttgagcgc ttcggtcttc tgtgccccgc ccttctccgg
    18841 ggtcttgccg ttgtaccgct tgaaccactg agcggcgggc cgctcgatgc cgtcattgat
    18901 ccgctcggag atcatcaggt ggcagtgcgg gttctcgccg ccaccggcat ggatggccag
    18961 cgtatacggc aggcgctcgg caccggtcag gtgctgggcg aactcggacg ccagcgcctt
    19021 ctgctggtcg agggtcagct cgaccggcag ggcaaattcg acctccttga acagccgccc
    19081 attggcgcgt tcatacaggt cggcagcatc ccagtagtcg gcgggccgct cgacgaactc
    19141 cggcatgtgc ccggattcgg cgtgcaagac ttcatccatg tcgcgggcat acttgccttc
    19201 gcgctggatg aagtcggcct tggccctggc cgattggccg cccgacctgc tgccggtttt
    19261 cgccgtaagg tgataaatcg ccatgctgcc tcgctgttgc ttttgctttt cggctccatg
    19321 caatggccct cggagagcgc accgcccgaa gggtggccgt taggccagtt tctcgaagag
    19381 aaaccggtaa gtgcgccctc ccctacaaag tagggtcggg attgccgccg ctgtgcctcc
    19441 atgatagcct acgagacagc acattaacaa tggggtgtca agatggttaa ggggagcaac
    19501 aaggcggcgg atcggctggc caagctcgaa gaacaacgag cgcgaatcaa tgccgaaatt
    19561 cagcgggtgc gggcaaggga acagcagcaa gagcgcaaga acgaaacaag gcgcaaggtg
    19621 ctggtggggg ccatgatttt ggccaaggtg aacagcagcg agtggccgga ggatcggctc
    19681 atggcggcaa tggatgcgta ccttgaacgc gaccacgacc gcgccttgtt cggtctgccg
    19741 ccacgccaga aggatgagcc gggctgaatg atcgaccgag acaggccctg cggggctgca
    19801 cacgcgcccc cacccttcgg gtagggggaa aggccgctaa agcggctaaa agcgctccag
    19861 cgtatttctg cggggtttgg tgtggggttt agcgggcttt gcccgccttt ccccctgccg
    19921 cgcagcggtg gggcggtgtg tagcctagcg cagcgaatag accagctatc cggcctctgg
    19981 ccgggcatat tgggcaaggg cagcagcgcc ccacaagggc gctgataacc gcgcctagtg
    20041 gattattctt agataatcat ggatggattt ttccaacacc ccgccagccc ccgcccctgc
    20101 tgggtttgca ggtttggggg cgtgacagtt attgcagggg ttcgtgacag ttattgcagg
    20161 ggggcgtgac agttattgca ggggttcgtg acagttagta cgggagtgac gggcactggc
    20221 tggcaatgtc tagcaacggc aggcatttcg gctgagggta aaagaacttt ccgctaagcg
    20281 atagactgta tgtaaacaca gtattgcaag gacgcggaac atgcctcatg tggcggccct
    20341 cgcgagaatt aattcagata aaaaaaatcc ttagctttcg ctaaggatga tttctagcga
    20401 tgaccctgct gattggttcg ctgaccgtag actggttggc ttggtttcat cagccatccg
    20461 cttgccctca tctgttacgc cggcggtagc cggccagcct cgcagagcag gattcccgtt
    20521 gagcaccgcc aggtgcgaat aagggacagt gaagaaggaa cacccgctcg cgggtgggcc
    20581 tacttcacct atcctgcccg gctgacgccg ttggatacac caaggaaagt ctacacgaac
    20641 cctttggcaa aatcctgtat atcgtgcgac atcgcgcgcg cgcgcgcgga tgctagcggc
    20701 gcgccaaaaa aaagcccgct catta
//
