LOCUS       sequence-463809-       21570 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Eggerthella lenta DSM 2243 El Vadh in Gordonibacter 
            urolithinfaciens DSM 27213.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 21570)
  AUTHORS   Dong X, Bae M, Le C, Aguilar Ramos MA, Balskus EP
  TITLE     Enantiocomplementary Gut Bacterial Enzymes Metabolize Dietary 
            Polyphenols.
  JOURNAL   J Am Chem Soc. 2025 Mar 5;147(9):7231-7244. doi: 
            10.1021/jacs.4c09892. Epub 2025 Feb 24.
  PUBMED    39993729
REFERENCE   2  (bases 1 to 21570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.4c09892"; journalName: "J Am Chem Soc"; date: 
            "2025-03-5- 5"; volume: "147"; issue: "9"; pages: "7231-7244"
COMMENT     Sequence Label: pXD80ENSF6-Elen0497-ElVadh-Accs(ElDSM2243)
FEATURES             Location/Qualifiers
     source          1..21570
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(19..813)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     primer_bind     1300..1318
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    1475..1494
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     terminator      15194..15221
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      15353..15380
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(15812..16663)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(16650..17489)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(18000..18971)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELSERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     oriT            20210..20297
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     rep_origin      20639..21033
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     terminator      complement(21196..21239)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     oriT            21335..21444
                     /note="incP origin of transfer"
ORIGIN
        1 ggcgggctgg ccggccttct aaaacaattc atccagtaaa atataatatt ttattttctc
       61 ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg acatactgtt cttccccgat
      121 atcctccctg atcgaccgga cgcagaaggc aatgtcatac cacttgtccg ccctgccgct
      181 tctcccaaga tcaataaagc cacttacttt gccatctttc acaaagatgt tgctgtctcc
      241 caggtcgccg tgggaaaaga caagttcctc ttcgggcttt tccgtcttta aaaaatcata
      301 cagctcgcgc ggatctttaa atggagtgtc ttcttcccag ttttcgcaat ccacatcggc
      361 cagatcgtta ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc tattcgtata
      421 gggacaatcc gatatgtcga tggagtgaaa gagcctgatg cactccgcat acagctcgat
      481 aatcttttca gggctttgtt catcttcata ctcttccgag caaaggacgc catcggcctc
      541 actcatgagc agattgctcc agccatcatg ccgttcaaag tgcaggacct ttggaacagg
      601 cagctttcct tccagccata gcatcatgtc cttttcccgt tccacatcat aggtggtccc
      661 tttataccgg ctgtccgtca tttttaaata taggttttca ttttctccca ccagcttata
      721 taccttagca ggagacattc cttccgtatc ttttacgcag cggtattttt cgatcagttt
      781 tttcaattcc ggtgatattc tcattttagc catgatgttt aactttgttt tagggcgact
      841 gccctgctgc gtaacatcgt tgctgctcca taacatcaaa catcgaccca cggcgtaacg
      901 cgcttgctgc ttggatgccc gaggcataga ctgtacccca aaaaaacagt cataacaagc
      961 catgaaaacc gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt
     1021 gcgtgagcgc atacgctact tgcattacag cttacgaacc gaacaggctt atgtccactg
     1081 ggttcgtgcc ttcatccgtt tccacggtgt gcgtcacccg gcaaccttgg gcagcagcga
     1141 agtcgaggca tttctgtcct ggctggcgaa cgagcgcaag gtttcggtct ccacgcattc
     1201 gaaaccggta tcccgccgcc gccgccggat accggtttcg aattgagatt gccacctgac
     1261 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     1321 tttcgtcttc aaggcgaaaa aaccccgccg aagcggggtt ttttgcgaaa tataatgacc
     1381 ctcttgataa cccaagaggg cattttttag gaacggttat ttgattatta atccggcggc
     1441 aactgaaacg attcggatcc tgtattacta ttctgaattc gcggccgctt ctagatttac
     1501 gcgacttctt cctctgagcc ctccaccagg tccatcagct cttgctgggt gtggatgtcg
     1561 agctttttgt agatgtggcg catatgggtg cgggcggtgc cctcggagat gtagagctgc
     1621 tcctggatgt aggcggcgtt gcggcctttc gccagcagga acaggacttc tgtttccttc
     1681 ttcgacagga ggtagcggtt agccaccagc tcgcacttgc gcttgaaccg cccggcgcgc
     1741 gctttttcgg gggactcggc gcgctcgcgc ccgcggagca tctcgcctgc cgggttgtcc
     1801 gattcaccca cgatgagggc gcgcagctcg ctgccgcgcg gcagggcgcc gaagccgaaa
     1861 atgatgcatg cgaacacgat gatcagcgtg ttcgccggat tggttgcgga gtcgagcgcg
     1921 gggatggcga aggaggccgt catcccgatg agcgcgccca gcgcgagtgc cccgcgtccg
     1981 aacccgaaca ccacgaacga cgtcaggcgg aaacgctggg tgatgtcggc gtagctcacc
     2041 cacatggtgc cctcgaacag gaggtagcac agcagcagcg cgaacgtggt gaagggcgtc
     2101 gcgtcggcga taaagtagac cgcgaccagc gcgatcgcga taaggggcag cagggggcgg
     2161 aacatgaagt tgatgtgctg gcgctgcgtt agcatgacgg cgatcatgag cgcgcacgcc
     2221 gtgagcgcgg ccgccccgat gcagacgacc tgcgttccca ggccccctac caagtgcatg
     2281 tcggcgatgg cttgcgtgcg cacggcgccc agcgcgaacc cgaacagcag gctgggcgcg
     2341 cacaggcgca gcgcgaacgg ggttttcctc accttgagcg tgatggcggc gaactcgagc
     2401 ttgtcgacga gcgcggtgct gcaccggtag aggcaccagc actcgaggaa cgggatgagg
     2461 gccgcggcca gggctcccag cggatggagc aggtccaggt tagacactgc cgtgtacagc
     2521 gcgatgcccg ccaccagcga gagcgccgta ctggtgacga tggtggcgat gtcgcattgc
     2581 ccgaacgaca cgccgtagct catcaggagg atggccgagc cgatgccggt ggtcgcacct
     2641 cccgccgcga gcgcggcgat gcccgctgcg cttccgacgt ccgccaagca catgaacagc
     2701 gtgccggcga aaccgagcgt cgcgccgatg aaccgtaggc gtgcgcgtcg gcgctgcgtg
     2761 gcgaacaaac ggcggtatgc gcgcgatagc acgccgtagg acagcagcgt catgacgaag
     2821 aacacgagcg acacggggtg gaagagcccg gccggcaccg ccggatcggc cgcgttccag
     2881 aagaacagct gctggccgtt gaacatcgtg aggtagaccc acgcccagtg ggcgccgaag
     2941 ccgacgatga gcgctggcga cagctttccc agcgagaacc cctcatcctc gcgcacctcc
     3001 actttgaccc gcggaagctt gatttccatc aggtacccgc tttcccctcg tctgcgaacc
     3061 cttgctgttc atcatactgc accgtcatcg ggttgctgct ccgcgcctgc cacctttccc
     3121 ctttgtcgat ggctgtttcc tggggttttg cgaaaaatca tagccacggt atgatgcccc
     3181 gccactccgt ctcgacgcaa aatcatagcg ctgctgggag gggatgcccg ccttcgccgc
     3241 ttaggatggc tccatcgttt caggtatgaa gcgagagacg agaaaggaag gtggggatca
     3301 atgactcagc tcacgctcac gcgccgcagc ttcatgaagg cgatggcggt caccggtgcc
     3361 gctgcgtcgc tcacggcggt cgccgagccg atgcgggcgc ttgccgaggg cgtcgatgcc
     3421 gatgcggggg aagtcaagcg cgttcgctcg tgctgccgcg cttgcggcaa ggtggagtgc
     3481 ggcgtttggg taaccgtgcg cgacggcaag gtcgtcaagg tggaaggcga cgagtccgcg
     3541 ccgcaaagcc gcgggcactg ctgctcgaag tcgcagtcat cgatgcaggc gctatatcat
     3601 cccgaccgcc ttcgtttccc ggtgaagcgc acgaacccta agggcgagga cgatcccggt
     3661 tgggtgcgca tcacgctgga cgaggcgttc gagatatgcg gcgagaagct caaggaagtg
     3721 aaggagaagt acggcgggga gtccatcttc gtgatgtgcg gcacgtcgcg cgtgtggtcg
     3781 ctcgggcctt accagggcat gaagcagctg ttcggcacgc cgaacgcgca tttggcctac
     3841 caggtgtgca aaggcccgcg ccacttcggc ggcatcatga ccgacgagat ggggtccccg
     3901 tggatggagg tggaggccga gcccagcgtg tacgtgcagt ggggcacggc gtgcgagtac
     3961 tccaactacg actccacgaa ccgcacggtc accgacgtcg cgcatcgtgc gtctaagcat
     4021 atcgtggtgg atccgcgcgt cacgcctttg ggcaaggaag ccgacatctg gctgccgctt
     4081 cgtcccggca ccgacggcgc gctggcgctg tcgtggctca actggatcat cgaaaacgaa
     4141 gcttacgatg acacgatggt gcgtcgctgg tcgaacgcgt cgttcctcta cgtggacgac
     4201 aagcccgagc tgacgcaagg ctggttggtg gagggcaacg gcggcatcaa catgaagacg
     4261 aagctgctga ccgaggccga cctcaaggaa gatggcaagt accagcgctt catggtgtgg
     4321 gacgaggcga acgagcgtct cacgtactgg gatgccgagc ttggcatgtg ggagggcgag
     4381 gagcaccgca tccccaccac cggcacatgg atcgagcatc cgtacaagcc gcttgttgcc
     4441 gatgcatggc tgcccgatca gtcgacgttc gccgatccgg ctaccgagcc cgaccgcttc
     4501 cccgacggtt tcgaggaatg caatcccaag gggttgccga agcgcccatc tctgcttcct
     4561 ggtgaggtag aggtaacgct caaagacgga accgtgcaca aggctcgctc tgtgtgggat
     4621 gcgttccatc agatgaccag cgagtacacc atggagaagg cagaggagat caccggcgtg
     4681 ccggcggcga agagcgagga ggctgtacgc gcctggacga cgcgcgtcaa tccgctgcat
     4741 ggcaacggcg gcatccactt ccagctggcc accgaccaga acggcaactc catccagaac
     4801 gtgcgtgcgc tgcagatcct gtcgtgcatc accggcaact ccgacgagcc ggccggcaac
     4861 cgcggctcgt cgaaggcgca gttcgacggc aatccagggc gttcgaacat gcaggccggc
     4921 gcgccgtacg gcgacgaggc caagatgtgg gacggccgcg acgtcgacct cgaagggctc
     4981 gccgcgaaga tgcaggattt cgtgcagtac ctcatcgaca acgattcgcc cctggccgag
     5041 cgctacggca acaaggtgcc cagccatgag gaagccctcg ttatcgccaa gcgcatgggc
     5101 ggcgcattcc gcacgtcgca ggcgtggccg aaccccaaga ccgtgttcga gcgacaagcc
     5161 aacgaggttg acgccgagcg attccccctc aaccgctact gggcgcgctg ggcggacgcg
     5221 aactccatct gggacggctg cctggacaac gacgtgccgt accagttgca tgcgggcgtg
     5281 tgccagtcgg gcgacttcat gaatatgggc aacatcgacg tggcgtggga ggccatgacg
     5341 aagctggact tcttcaccga catcaacctg tggttctgcc cgaacaacgg caacgccgac
     5401 gtcatctttc cgtgctacca ctggttggaa gttgacacga cgcgcgtcag ccagggcgcc
     5461 ggcgggttct tcggctgcgg gtgcgccgct gtcgaggcgc cgggcgagtg catctacgat
     5521 cccgactgga acgtgggcat gtacaaggcc atgggcgtgc catggaacac gaaggacgag
     5581 tcggccgagc cgtccgagat cctcaacttc ggcgagaaga ccgactaccg ttggccgaac
     5641 cgcagccgcg tgctcaagga caacgtggac gcgtggaaga cagccgagtt ccccgacggt
     5701 cccacgtggg agcaagcgaa ggagcacttc cagaagaacg gctggatgga ttgccgcacg
     5761 tggcatcccg agcgttgggg cacgtatcgc cggcacgaga tgggctggcg tcgccagcag
     5821 ggcggcttca acctgttccc gttggtggac gaccatccgg gcttcatgac gccgtcgggc
     5881 ctcattgaaa tctggtcgct tgtgtgcgag gcgtacttag gcgacgagga caagttcccc
     5941 gtgtaccgcg aacccacgaa ctcgccggtg accacgcccg agtacttcga cgccgcgaag
     6001 gttgccgaca tcgatgagtc gaagctgcgc aatatggatt atcccgccag cctgcaggag
     6061 cacgccgatg cgacgttcct catgaccacg ggcgcgcgcc agccggtgta cttccacagc
     6121 gagcatcgcc agctgccgtg gtgccgcgag ctgtggcccg cgccgcgcct ggagatgaac
     6181 ccgaacgacg ctgcgcgtct ggggctcgag cagggcgact gggtgtggat cgagagcccg
     6241 tggggcaagg tgcgcgaggt gctggatctg tactacggca tcagcaaggg cgtggtgaac
     6301 gcgaaccacg cgtggtggtt ccccgagatg gacacggcca gccacggcta cgagctggtg
     6361 aacatcaact gcgtgatgga tccttacggc caggatgtgg tgtgcggtgc agcaacgatg
     6421 cgctcggttc ccgtgctggt ttataaggct acggcggaaa actcgccgtt cggcaacccg
     6481 gtgccgtgcg atccgcaggg taacccgtgc atctgcgatg cgggcgatcc gcggttgaag
     6541 gaatggatgg gaacgggatt gcgatctcgc gttgaaggcg aagaggcgtg gaccggagag
     6601 ggggcgtaga cgatgacaca gtacgcaatc atcaccgatt tgaaccggtg cgtcggatgc
     6661 cttgcgtgca cggtcgcatg caaagcggtg aacaacgtgc ccgtcggggc gttttggatc
     6721 aagacgctgc gcgtgggtcc gaatccgatc gagggcggaa gcggcgattg gcccgacgtt
     6781 gagatgtatt tcctgcccat ccaatgccag cattgcgccg atcccgagtg cgtgaaggtg
     6841 tgtcccacgg gcgcctcgca taaggcagcc gacggcaccg tgcagatcga caagtcgaag
     6901 tgcattggct gccagttctg cgcgatgagc tgcccctaca acgttcgcta cctcaacgaa
     6961 gaggagcgcg tggtggagaa gtgcacgctg tgcgagcaga agatcgccca aggcgagctt
     7021 ccgcagtgcg tggcgcaatg cggctcgcgt gcgcgcttct tcggcgactt ggagcagggc
     7081 gtggacgggt tcgaggcacc ggctccgcct caagccctgt cgtgcgacta cgccgaaatg
     7141 cagcagacgc gcgtcacgct gaaggagtac gtcgaaccgt atacggaaga cgagatccat
     7201 cgtctgcccg atgttggaaa tggcccggag ctcatgtacc tgctgcgctc cgaccgaaag
     7261 tggagggggt agaccatgga actgcaatgg cctttgatcg tgtttaccac cctggttgcc
     7321 tggtcggcgg ggctgttcgg gacgcaggcg ctgatggcgg tgttcggcgt cgggaagaag
     7381 gcgcaggtgc cggcttgggt ctgctcggcc gtgctgctgg cggccggcgg gatcgcggtg
     7441 ttcttccatc tcgagcactg ggagcggata ttcaacgggt tcgggcacct gacgagcggg
     7501 atcacccagg aattgatcgc gatcatcgtg cttgccgtgg tggctatcgt ctacttggtg
     7561 ctcatgcgca agtccgacga cggcgcgagc gtgccgaagt ggctggcctg ggtcgccgtc
     7621 gcgctgtcgg tggtgctggt cgccgtgatg gcgcactcgt acacgatggc cgcccgcccc
     7681 gcctgggact cggccctctg gatcctctac gtcctcggca acgcctgcgt gttgggcccc
     7741 gcgacgttcg ccctcctctc cgccttggct gcagggggac cccgggacca gcccgccgag
     7801 cgcgcagccg acgccggggc ccccgcgggt aggacgcccc tggtaggcgc agccgccaac
     7861 gccctcgcaa ccctcgcctt cgccgccttc ctccagctct ccgccggctc cttcgccgac
     7921 gtcggcctct acttcgaccc gacgcacccc accaaggcca tggccgacgc agccgcgacg
     7981 gtcgcctccc aggccccgct cctgtggctg ggcgccgtgg ccgtcggcgc gatcgtcccc
     8041 ctggccgccg ccttcctagg caggcgcacg gggaactgga agctctgggc cccggcagcc
     8101 ataatcgccg ccctcgtcgg agccgtctgc atgcgcgtcg tcttctacaa cctggggctc
     8161 tcggtcttca tgttctacta gactagtccg ctggttgcca cgacgccctc gaagtctcct
     8221 tcgagggcgt tctgtcgttt gcaagaaggg tctggagagg caaatcgcgc tcgaaggggc
     8281 ttcgaggccg gggtaggtcg ggagggtgga atccggatgt ttcacgtgaa acatccgttc
     8341 ttttacggcc ggtgtttcgc gtgaaacatt ttgcggaaag gactccttcc cttcccgtgc
     8401 gccaccccaa cgccccgctc ctagcgctcg tacacctggt cgcagttgag cagatccttc
     8461 atggagtgcc agatctggtg cgggttgccc ggcgggttct tgagcggctt catcggatag
     8521 cgctcctgcg cgcccgacag cagatcgacg gcgaacactt cgtcggacag ctcgagcgcg
     8581 ccttccgggc agatgtcggt gcagcagcgg cacttcacgc aatcgaccgg acgatgcgtc
     8641 acgccgatcg agccgtcctc acccgcgtac ttcgcgatgg ccccggtggg gcagaacgtg
     8701 gcgcacatct ggcacgacga gcacttctcg ggatcgatga tcacatggcc ccacaagcgc
     8761 gtgtccatga gcacatcctg cggctgcccc aacgaatcga gcgcggccag caaacgttcg
     8821 cggcggtcgg gaatgaagtg aggcagcgtg ccgtccttgc ccaccttcac gaacctcggc
     8881 tcccgccgct cctccacacc caatgcgtcc ttgacggcgt agtcagccgt caccgccgca
     8941 acgttcttag cctcgtcctt catgctggag aagaagctcc gacggctcgc gtcgtagccc
     9001 tcgtcgcccg cccggcgcac ggatgaggga agcttttccg ccaggtcgat gcgcatatcg
     9061 ttgttccagg ttgcaagcag cgcgcttgcc gtgtcgcaca caacccgcgc catctcgaaa
     9121 cccgagacat gctcgcattg cgcgcagcgc gcctgcacga ggctcacgcg gcgcacgccc
     9181 aacgccgcca gcgtgaccag caggctctcc tccacgcgcc ccaggcacgt gacgcccacc
     9241 accttctcgg ggtcgtacaa cccgtcggcc gcggcaagca gctgatcgca ggcgataacc
     9301 acctgtccgt cggccacgtc agccgccgcg cggcatgctt gcagaagctc ggcatcgttc
     9361 ggacgatgcg cttcaagcgc gcaggtggga cacacggtgg cgcaggttcc gcacccgatg
     9421 caccgctcgg gttcgatgac cagctcgttg tcgtcatagg agatgcaacc gctcgtgcac
     9481 acgtcggcgc acttcatgca cgttgcgttg cgattgcgca caatagcgca acgaccctga
     9541 tgaaccgtaa tgtcggcgct ctgaagttgt tcgagcagac cgaggaaatt cgtcacgtga
     9601 gccatgactt caccctttct cgtctacccc cttgcaccgc acagtataac cactttcgcc
     9661 gtctcacgcg agaaaaaacg cgcttgacac cttcgacagg cctccctatc atgtggcctt
     9721 ggggagtccg ccggcacgct gatcgcgtgc gaagcggaca ttgtttgcaa aagtgggaac
     9781 ctcaggggtg aggtttcgcg gtgctgtgcg ccgtaagtgc aaacaatgtc tgacgagggg
     9841 cggaacatga ctcacgattc cacgaaccag catctttctc ttgagcccga ctacgacggg
     9901 ttcgatccac ttgaagattt cgaagacgat gcgaccgagg tcgaaggccg tgccgacgag
     9961 ttcgtcgcca cggtgagcgc cgccgaagac gcgctcgctc acgacgcgat ggcacccgcc
    10021 acgcccgcca tctgcgaaaa gcccgctgaa gagcgcatcg ccgacttgtt ggaggccatg
    10081 gcgccgcgcc gcacggtgct gctgggcatc ctggccgcct gcgccgagcc gcagccggca
    10141 tccgccgtga acgctcgcgt cgacgagctg caggagaaca acttctcggt gtacagcgcg
    10201 gccaacctgt gctccttgct ggagaaggcg ggcgccatcg agcgcgtcac ggccgagggc
    10261 gaacccgcgg aggacatcga aatcgagcca cagaccgtcg tcgtcgacgg cgtcgaatac
    10321 ctggaagccc gcgaacccgt cgagatctac tggcacatca ccgagccggg ccgcgcggcg
    10381 cttgaggccg acaaaccgct cgagcgcctg cgcgccctcc tggacgaaga tgccgcctac
    10441 gcgcccatct accagcgcat cctccgcttg tgcacagcag acggcggcgc cacgacaccc
    10501 gccatcaaca acgcggtcga ccacaacccg ctggtgcaga agccgcgctt ctacgcgccc
    10561 cacttcgtcg accgcctgga gaaatgcgac gcgctggcct ggaagaaggc gtggtgcatc
    10621 accgacatcg gacgcgccgg cctcgatatg ctggcagacg tcgtagacga gaacgcgccc
    10681 gccacccagc ccgaaacccc cgcaacccct gatcccgccg catccaagga ggactaacca
    10741 tgaccgaagc aaccgcgccc gcaaccgaga ccctcgccga cgaagcgacc atgcaggagc
    10801 tcgtcaaact gaacgagcag cgcgccgcca cgtacggcct gctgtcgcgc ctgtaccgcg
    10861 tggaggtgga ccagcccctt ctcgacgagc tgcgcagcat gcgcttcccc gccaagacgg
    10921 gcaacgagaa cgtggacgag ggctaccgcc tgctggccac gtacctgagc aacacctggg
    10981 acaacagcgt caccgacctc gccatcgact acgtgcgcgt gttcatcgga cacggcatcg
    11041 acgcgttctc ggccgcctac ccgttcgaat cggtgtacac ctccgagaag cgcctgctca
    11101 tgcaggaagc acgcgacgag gtgctggcca tctaccgcag cgcaggcctc gacaagcagg
    11161 acacatggaa agagggcgag gatcatatcg cgctcgagct ggaattcgag cagatcatgg
    11221 ccacgcgcac ggtcgaagcg ctgcgcaaag gcgacgaaga cgaggccgtc gcgctgctga
    11281 ccacgcagaa gaactttctc gaagaccatc tggcggcatg ggctcccatg atgacctccg
    11341 acatgaagcg attcgcgcaa accgacttct accggggctt ggcgtacttg acggacgggt
    11401 tcctccaaac cgaccaaacg ttcctcgacg acgtgctggc cgaagactaa ctgcacagga
    11461 tcgaaagaag gagcgatgga caacaagcag gagaaaacct acgccggcat gacgcgacgc
    11521 acgctgtgcc tgggcgcagg cggcgctgcg gtcatgctag gtctaggcgg attgaagtac
    11581 gcgggatcgc aaccgctcgt gcgcccgccg ggaggccagg acgaggaacg gctcatctcg
    11641 gcctgcatcc gctgcgagaa gtgctacgag atctgcccgc gcgacgtcat cgcgcccgcc
    11701 cacctcgagg acggtgtgct gaacatgcgc acccccacgt tcgacttctc ggccaactgg
    11761 tgcgactggt gcgccgaaga gcacgacggc accccgttgt gcgtgagcgc ctgccccacc
    11821 gaagcgctca agctgcccga cgacgccacg gccgagaaca ccatcatcgg caaagccgtt
    11881 atcaacacgg actggtgctt ggcgtacaag ctgatcggat gccggttttg ctacgacgcc
    11941 tgtccctacg aggccatgga gctcgacgag aacaaccgcc ccgtcgtgat cgaggacaag
    12001 tgcaacggct gcggcgcgtg cgagagcgtg tgcgtctcgc tgcagaacgg ctccatctcc
    12061 gaaggagcca ccgcgcgcgc catcgtcgtc cacccgcttg acgaagttga aggataaggt
    12121 gccctcatga agaagaactc caagaagctg cgcaccctcg tcgcactggc catcgtggcc
    12181 gtcgtcgccg tcggcttcgt cacgaacgtc gggatcggca ccatgtcggc accgggcatc
    12241 tgggacatct ccatcctatg cccgctgggc gccttgggaa cgatgctcgc ctccaagatg
    12301 atggtgccgc gcgccgtaat ctcgctgatc gtgatggtgc tgctcatcgt tctgttcgcc
    12361 cgcgcgttct gcggatggat ttgccccgtg ccgctcgtgc agaagttgcg cggcttgttc
    12421 gcgaagaagc aacccgtcga acgcggcgac gatgccgatc ctgccgcggc gccgctcacg
    12481 aaagtcgaga tcgacgcgct gggcgtttca tgctcgaagg ggtcgaagaa tggcagcgga
    12541 tgcgcgtcct gcgcctccaa gcgcggagcc gcgctcgatg ctcgccactt cgtgctgggc
    12601 ggcgcgctgg cgtccacctt catctttgga ttcccggtgt tctgcctcgt ctgccccatc
    12661 ggactcacgt tcgccaccat cctgctggtc atcaacctgt tctcacacgg cgatgtcacc
    12721 tggtcgctca tcgtggtgcc cgcgctgctt atcgccgagg tgctgctgtt caagaagtgg
    12781 tgccataagc tgtgcccgct ttcggcgttc atgagcctga tcgcgaaggc gaacaagacc
    12841 ttcgtgccca cgatcgacga tgcgaagtgc ctggaaacgg cgaagggcgg cgcgtgcggg
    12901 gcctgcggcc gcgtctgcga ggagggcatc gacccgcgcc acccgcagct ttcgcaggcg
    12961 gcatggagcg agtgcaccaa gtgccgcgcc tgcgtggacg catgccctgc gaacgcgatc
    13021 accatgccgc tgctgccgcg gcgaacgccg tctaccgcag aacctgtcgc cgtgccaacg
    13081 aacgccgaga agatcgacgc ggagaatgcg cgccccgttc cgtaaagccc ctcctgcgcg
    13141 acccggctcg cagctgcagc ggtctggtcg cgcatccgga atcgggccga gcccgacccc
    13201 gggcaaaagg cggcttggca agggctttgg gggacgagcc gccttttgcc cgggagcgac
    13261 atccccgctc cccctccctg cgaaaagagg gatgccgctt ccggccttca tctgacaacc
    13321 acgacgtggg aggacgaaaa cccatggcat acgaaacgac aagcgaatcg accgaggccc
    13381 tgctcgtcac cggaggcgtc ggcaccggca aaacgcaacg tctcgtcgag cgcgctgccg
    13441 cgctgctggc cgaaaacgcc gccgcgggca acgtgctcgt gctgtgcgcg acgccccagg
    13501 ccgcacgcct gttctcggag cggttgacag cccgcgtcgg tgccaagcgc gctgccggcg
    13561 caaccgtcgc caccgcacgc gccttagcgc tcgacgtgct ggccgacgac ggcgcgcgcc
    13621 gctggtcggg gcgcgagccg cgactactga ccgccttcga agagacgttc ctcatggagg
    13681 acatgaaggt gagcgggctg cggccgaagc ggctgcgcga gatgctcaag ttcttctacc
    13741 gctcgtggac cgagctggcg gacgacgatc ccgattggct gctaccgggc gaggaggccg
    13801 atgtccatac gctgttgaaa gagaacctgg tcttcatgcg cgccgtcata gaacccgaag
    13861 cggcgaacct tgctgcacgc tacctccgag aacacgacga agcgcgggcg gcgcattcct
    13921 acgcgcacgt gctgatggac gactacgcct gcgcaagccg tgcctcgcag acgctggcga
    13981 atctcgtcgc aacctgctcg atcaccgtgg cgggcgacgc ggaaacctgc atcgagacat
    14041 acgattcgta cccctatgcc gccggaatcg acgaattcct cgcaacgcat cccgatgctg
    14101 cttgcgagca cctggatgcc gacgcgcccg atgccgtcga acgggtcgct ctggcaagcg
    14161 acacgcccga agccgagttc gaacaggtgg cgcgcacggt ggccgaagcc gtcgccgcag
    14221 gaacgcccgc acggcagatc gtcgtggccg taccgaacag cgtgtggtcg cgcaacatcg
    14281 caaccgcttt gcacgcacga ggcgtgccaa ccgaagcgct cgccatctcg cagcccctgc
    14341 gcggcgacgt gcgggacaac gcccgctgca cgcctgcgcg catcgccacg gccctcaacc
    14401 tcgtggcgaa cccagacgac accgcggctt ggcggtgctg gtgtggattc ggcgactacc
    14461 tggtgaacag cgcggctttc gccagcctgc ggacttgcgc gaaggagcgc agtatcggcc
    14521 tcgtggacgc tttggagatg ctaagcgaaa acgactgcga gcacgtagtg ggcgcgcaac
    14581 gcgtggcgac cgcctacaag gacggtcgcg cgctcatcga gcaggtacga ggattgcggg
    14641 gcacggcact gctggacgag ctgacgcgca tcgtcaccga cggagcggaa tcccaagcgc
    14701 ccgccgttct gcgcgagctc tgcctcgagc acggcaacgc agccgatgaa aacccggaaa
    14761 gcctttacga tgccgatgct gcggcgatgg cgcggcgcat ggccgagcgc ctgctcgcgc
    14821 ccacgctgga ggttgccgac gcggtagccg tcgtccccta cgacttcgcc tgcggcttct
    14881 cgcccgacct gctgatcgtc gccggcttcg tgaacggttt catcccctcc cgcgactatt
    14941 tcgacgccac ggtgaccccg cccgagcgcc aggagaagat gcgcgccgtc gacgaacgac
    15001 gcgtgcgcgc gctcacagga aaggccggcc gcgcggtggc gctgtcgtac ttcacgtcca
    15061 ccgacctcga aagcgccggc aagctgaagc tcaagatcag tcgcatccgc ctcaagaacg
    15121 gtatccgcac ctgcactata gagcccagcc tattcctgga gcaaatcgca gcgcaggagt
    15181 agcgactcac actggctcac cttcgggtgg gcctttctgc gtttatatac tagagagaga
    15241 atataaaaag ccagattatt aatccggctt ttttattatt taattcctgt tataaaaaaa
    15301 ggatcaattt tgaactctct cccaaagttg atcccttaac gatccctgca ggagaaggcc
    15361 atcctgacgg atggcctttt tgaagctttc tagaatcggg ggcccccggg gggattctag
    15421 aacaatggaa gtcctcgtct cgccctcgaa ttagcccgct taatgagcgg gctttttttg
    15481 aattaattct cgcgagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
    15541 acgcaattaa tgtaagttag cgcgaattgc aagctggccg acgcgctggg ctacgtcttg
    15601 ctggcgttcg ggagcagaag agcatacatc tggaagcaaa gccaggaaag cggcctatgg
    15661 agctgtgcgg cagcgctcag taggcaattt ttcaaaatat tgttaagcct tttctgagca
    15721 tggtattttt catggtatta ccaattagca ggaaaataag ccattgaata taaaagataa
    15781 aaatgtcttg tttacaatag agtggggggg gtcagcctgc cgccttgggc cgggtgatgt
    15841 cgtacttgcc cgccgcgaac tcggttaccg tccagcccag cgcgaccagc tccggcaacg
    15901 cctcgcgcac ccgctggcgg cgcttgcgca tggtcgaacc actggcctct gacggccaga
    15961 catagccgca caaggtatct atggaagcct tgccggtttt gccggggtcg atccagccac
    16021 acagccgctg gtgcagcagg cgggcggttt cgctgtccag cgcccgcacc tcgtccatgc
    16081 tgatgcgcac atgctggccg ccacccatga cggcctgcgc gatcaagggg ttcagggcca
    16141 cgtacaggcg cccgtccgcc tcgtcgctgg cgtactccga cagcagccga aacccctgcc
    16201 gcttgcggcc attctgggcg atgatggata ccttccaaag gcgctcgatg cagtcctgta
    16261 tgtgcttgag cgccccacca ctatcgacct ctgccccgat ttcctttgcc agcgcccgat
    16321 agctaccttt gaccaccatg gcatcagcgg tgacggcctc ccacttgggt tccaggaaca
    16381 gccggagctg ccgtccgcct tcggtcttgg gttccgggcc aagcactagg ccattaggcc
    16441 cagccatggc caccagccct tgcaggatgc gcagatcatc agcgcccagc ggctccgggc
    16501 cgctgaactc gatccgcttg ccgtcgccgt agtcatacgt cacgtccagc ttgctgcgct
    16561 tgcgctcgcc ccgcttgagg gcacggaaca ggccgggggc cagacagtgc gccgggtcgt
    16621 gtcggacgtg gctgaggctg tgcttgttct taggcttcac cacggggcac ccccttgctc
    16681 ttgcgctgcc tctccagcac ggcgggcttg agcaccccgc cgtcatgccg cctgaaccac
    16741 cgatcagcga acggtgcgcc atagttggcc ttgctcacac cgaagcggac gaagaaccgg
    16801 cgctggtcgt cgtccacacc ccattcctcg gcctcggcgc tggtcatgct cgacaggtag
    16861 gactgccagc ggatgttatc gaccagtacc gagctgcccc ggctggcctg ctgctggtcg
    16921 cctgcgccca tcatggccgc gcccttgctg gcatggtgca ggaacacgat agagcacccg
    16981 gtatcggcgg cgatggcctc catgcgaccg atgacctggg ccatggggcc gctggcgttt
    17041 tcttcctcga tgtggaaccg gcgcagcgtg tccagcacca tcaggcggcg gccctcggcg
    17101 gcgcgcttga ggccgtcgaa ccactccggg gccatgatgt tgggcaggct gccgatcagc
    17161 ggctggatca gcaggccgtc agccacggct tgccgttcct cggcgctgag gtgcgcccca
    17221 agggcgtgca ggcggtgatg aatggcggtg ggcgggtctt cggcgggcag gtagatcacc
    17281 gggccggtgg gcagttcgcc cacctccagc agatccggcc cgcctgcaat ctgtgcggcc
    17341 agttgcaggg ccagcatgga tttaccggca ccaccgggcg acaccagcgc cccgaccgta
    17401 ccggccacca tgttgggcaa aacgtagtcc agcggtggcg gcgctgctgc gaacgcctcc
    17461 agaatattga taggcttatg ggtagccatt gattgcctcc tttgcaggca gttggtggtt
    17521 aggcgctggc ggggtcacta cccccgccct gcgccgctct gagttcttcc aggcactcgc
    17581 gcagcgcctc gtattcgtcg tcggtcagcc agaacttgcg ctgacgcatc cctttggcct
    17641 tcatgcgctc ggcatatcgc gcttggcgta cagcgtcagg gctggccagc aggtcgccgg
    17701 tctgcttgtc cttttggtct ttcatatcag tcaccgagaa acttgccggg gccgaaaggc
    17761 ttgtcttcgc ggaacaagga caaggtgcag ccgtcaaggt taaggctggc catatcagcg
    17821 actgaaaagc ggccagcctc ggccttgttt gacgtataac caaagccacc gggcaaccaa
    17881 tagcccttgt cacttttgat caggtagacc gaccctgaag cgcttttttc gtattccata
    17941 aaaccccctt ctgtgcgtga gtactcatag tataacaggc gtgagtacca acgcaagcac
    18001 tacatgctga aatctggccc gcccctgtcc atgcctcgct ggcggggtgc cggtgcccgt
    18061 gccagctcgg cccgcgcaag ctggacgctg ggcagaccca tgaccttgct gacggtgcgc
    18121 tcgatgtaat ccgcttcgtg gccgggcttg cgctctgcca gcgctgggct ggcctcggcc
    18181 atggccttgc cgatttcctc ggcactgcgg ccccggctgg ccagcttctg cgcggcgata
    18241 aagtcgcact tgctgaggtc atcaccgaag cgcttgacca gcccggccat ctcgctgcgg
    18301 tactcgtcca gcgccgtgcg ccggtggcgg ctaagctgcc gctcggacag ttcgaggctg
    18361 gccagcctgc gggccttctc ctgctgccgc tgggcctgct cgatctgctg gccagcctgc
    18421 tgcaccagcg ccgggccagc ggtggcggtc ttgcccttgg attcacgcag cagcacccac
    18481 ggctgataac cggcgcgggt ggtgtgcttg tccttgcggt tggtgaagcc cgccaagcgg
    18541 ccatagtggc ggctgtcggc gctggccggg tcggcgtcgt actcgctggc cagcgtccgg
    18601 gcaatctgcc cccgaagttc accgcctgcg gcgtcggcca ccttgaccca tgcctgatag
    18661 ttcttcgggc tggtttccac taccagggca ggctcccggc cctcggcttt catgtcatcc
    18721 aggtcaaact cgctgaggtc gtccaccagc accagaccat gccgctcctg ctcggcgggc
    18781 ctgatataca cgtcattgcc ctgggcattc atccgcttga gccatggcgt gttctggagc
    18841 acttcggcgg ctgaccattc ccggttcatc atctggccgg tggtggcgtc cctgacgccg
    18901 atatcgaagc gctcacagcc catggccttg agctgtcggc ctatggcctg caaagtcctg
    18961 tcgttcttca tcgggccacc aagcgcagcc agatcgagcc gtcctcggtt gtcagtggcg
    19021 tcaggtcgag caagagcaac gatgcgatca gcagcaccac cgtaggcatc atggaagcca
    19081 gcatcacggt tagccatagc ttccagtgcc acccccgcga cgcgctccgg gcgctctgcg
    19141 cggcgctgct cacctcggcg gctacctccc gcaactcttt ggccagctcc acccatgccg
    19201 cccctgtctg gcgctgggct ttcagccact ccgccgcctg cgcctcgctg gcctgctggg
    19261 tctggctcat gacctgccgg gcttcgtcgg ccagtgtcgc catgctctgg gccagcggtt
    19321 cgatctgctc cgctaactcg ttgatgcctc tggatttctt cactctgtcg attgcgttca
    19381 tggtctattg cctcccggta ttcctgtaag tcgatgatct gggcgttggc ggtgtcgatg
    19441 ttcagggcca cgtctgcccg gtcggtgcgg atgccccggc cttccatctc caccacgttc
    19501 ggccccaggt gaacaccggg caggcgctcg atgccctgcg cctcaagtgt tctgtggtca
    19561 atgcgggcgt cgtggcctgc ccgctctaat gcccggttgg catggtcggc ccatgcctcg
    19621 cgggtctgct caagccatgc cttgggcttg agcgcttcgg tcttctgtgc cccgcccttc
    19681 tccggggtct tgccgttgta ccgcttgaac cactgagcgg cgggccgctc gatgccgtca
    19741 ttgatccgct cggagatcat caggtggcag tgcgggttct cgccgccacc ggcatggatg
    19801 gccagcgtat acggcaggcg ctcggcaccg gtcaggtgct gggcgaactc ggacgccagc
    19861 gccttctgct ggtcgagggt cagctcgacc ggcagggcaa attcgacctc cttgaacagc
    19921 cgcccattgg cgcgttcata caggtcggca gcatcccagt agtcggcggg ccgctcgacg
    19981 aactccggca tgtgcccgga ttcggcgtgc aagacttcat ccatgtcgcg ggcatacttg
    20041 ccttcgcgct ggatgaagtc ggccttggcc ctggccgatt ggccgcccga cctgctgccg
    20101 gttttcgccg taaggtgata aatcgccatg ctgcctcgct gttgcttttg cttttcggct
    20161 ccatgcaatg gccctcggag agcgcaccgc ccgaagggtg gccgttaggc cagtttctcg
    20221 aagagaaacc ggtaagtgcg ccctccccta caaagtaggg tcgggattgc cgccgctgtg
    20281 cctccatgat agcctacgag acagcacatt aacaatgggg tgtcaagatg gttaagggga
    20341 gcaacaaggc ggcggatcgg ctggccaagc tcgaagaaca acgagcgcga atcaatgccg
    20401 aaattcagcg ggtgcgggca agggaacagc agcaagagcg caagaacgaa acaaggcgca
    20461 aggtgctggt gggggccatg attttggcca aggtgaacag cagcgagtgg ccggaggatc
    20521 ggctcatggc ggcaatggat gcgtaccttg aacgcgacca cgaccgcgcc ttgttcggtc
    20581 tgccgccacg ccagaaggat gagccgggct gaatgatcga ccgagacagg ccctgcgggg
    20641 ctgcacacgc gcccccaccc ttcgggtagg gggaaaggcc gctaaagcgg ctaaaagcgc
    20701 tccagcgtat ttctgcgggg tttggtgtgg ggtttagcgg gctttgcccg cctttccccc
    20761 tgccgcgcag cggtggggcg gtgtgtagcc tagcgcagcg aatagaccag ctatccggcc
    20821 tctggccggg catattgggc aagggcagca gcgccccaca agggcgctga taaccgcgcc
    20881 tagtggatta ttcttagata atcatggatg gatttttcca acaccccgcc agcccccgcc
    20941 cctgctgggt ttgcaggttt gggggcgtga cagttattgc aggggttcgt gacagttatt
    21001 gcaggggggc gtgacagtta ttgcaggggt tcgtgacagt tagtacggga gtgacgggca
    21061 ctggctggca atgtctagca acggcaggca tttcggctga gggtaaaaga actttccgct
    21121 aagcgataga ctgtatgtaa acacagtatt gcaaggacgc ggaacatgcc tcatgtggcg
    21181 gccctcgcga gaattaattc agataaaaaa aatccttagc tttcgctaag gatgatttct
    21241 agcgatgacc ctgctgattg gttcgctgac cgtagactgg ttggcttggt ttcatcagcc
    21301 atccgcttgc cctcatctgt tacgccggcg gtagccggcc agcctcgcag agcaggattc
    21361 ccgttgagca ccgccaggtg cgaataaggg acagtgaaga aggaacaccc gctcgcgggt
    21421 gggcctactt cacctatcct gcccggctga cgccgttgga tacaccaagg aaagtctaca
    21481 cgaacccttt ggcaaaatcc tgtatatcgt gcgacatcgc gcgcgcgcgc gcggatgcta
    21541 gcggcgcgcc aaaaaaaagc ccgctcatta
//
