LOCUS       sequence-463810-       21167 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Eggerthella lenta A2 Cadh in Gordonibacter 
            urolithinfaciens DSM 27213.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 21167)
  AUTHORS   Dong X, Bae M, Le C, Aguilar Ramos MA, Balskus EP
  TITLE     Enantiocomplementary Gut Bacterial Enzymes Metabolize Dietary 
            Polyphenols.
  JOURNAL   J Am Chem Soc. 2025 Mar 5;147(9):7231-7244. doi: 
            10.1021/jacs.4c09892. Epub 2025 Feb 24.
  PUBMED    39993729
REFERENCE   2  (bases 1 to 21167)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.4c09892"; journalName: "J Am Chem Soc"; date: 
            "2025-03-5- 5"; volume: "147"; issue: "9"; pages: "7231-7244"
COMMENT     Sequence Label: pXD80ENSF3-Elen0507-Cadh-Accs(ElA2)
FEATURES             Location/Qualifiers
     source          1..21167
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(19..813)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     primer_bind     1300..1318
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    1475..1494
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     terminator      7796..7823
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      14950..14977
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(15409..16260)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(16247..17086)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(17597..18568)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELSERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     oriT            19807..19894
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     rep_origin      20236..20630
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     terminator      complement(20793..20836)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     oriT            20932..21041
                     /note="incP origin of transfer"
ORIGIN
        1 ggcgggctgg ccggccttct aaaacaattc atccagtaaa atataatatt ttattttctc
       61 ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg acatactgtt cttccccgat
      121 atcctccctg atcgaccgga cgcagaaggc aatgtcatac cacttgtccg ccctgccgct
      181 tctcccaaga tcaataaagc cacttacttt gccatctttc acaaagatgt tgctgtctcc
      241 caggtcgccg tgggaaaaga caagttcctc ttcgggcttt tccgtcttta aaaaatcata
      301 cagctcgcgc ggatctttaa atggagtgtc ttcttcccag ttttcgcaat ccacatcggc
      361 cagatcgtta ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc tattcgtata
      421 gggacaatcc gatatgtcga tggagtgaaa gagcctgatg cactccgcat acagctcgat
      481 aatcttttca gggctttgtt catcttcata ctcttccgag caaaggacgc catcggcctc
      541 actcatgagc agattgctcc agccatcatg ccgttcaaag tgcaggacct ttggaacagg
      601 cagctttcct tccagccata gcatcatgtc cttttcccgt tccacatcat aggtggtccc
      661 tttataccgg ctgtccgtca tttttaaata taggttttca ttttctccca ccagcttata
      721 taccttagca ggagacattc cttccgtatc ttttacgcag cggtattttt cgatcagttt
      781 tttcaattcc ggtgatattc tcattttagc catgatgttt aactttgttt tagggcgact
      841 gccctgctgc gtaacatcgt tgctgctcca taacatcaaa catcgaccca cggcgtaacg
      901 cgcttgctgc ttggatgccc gaggcataga ctgtacccca aaaaaacagt cataacaagc
      961 catgaaaacc gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt
     1021 gcgtgagcgc atacgctact tgcattacag cttacgaacc gaacaggctt atgtccactg
     1081 ggttcgtgcc ttcatccgtt tccacggtgt gcgtcacccg gcaaccttgg gcagcagcga
     1141 agtcgaggca tttctgtcct ggctggcgaa cgagcgcaag gtttcggtct ccacgcattc
     1201 gaaaccggta tcccgccgcc gccgccggat accggtttcg aattgagatt gccacctgac
     1261 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     1321 tttcgtcttc aaggcgaaaa aaccccgccg aagcggggtt ttttgcgaaa tataatgacc
     1381 ctcttgataa cccaagaggg cattttttag gaacggttat ttgattatta atccggcggc
     1441 aactgaaacg attcggatcc tgtattacta ttctgaattc gcggccgctt ctagattcat
     1501 gccttcttct gctcgtcgat ggtctgctcg accagattga ggatctcctg ctggttgtgg
     1561 acgtccatct tcgcatagat gttgcgaagg tgggtcttcg cggtggactg cgtgatgacg
     1621 agcgtgtccg cgatatgctg cgcggtgcgg ccgcgtgcca gcaatacgag gatctcctcc
     1681 tcgcgccccg tcagctggta ctggtcggtg agcgcatggc acgcgttctg cagcttgttc
     1741 gcgtggggaa gcgcatcgtg aacgtcgggt gcgtcagggc cgggccattg accgcggatt
     1801 ggcgtgtggt agtcggtgat gcttcccgaa agcgacgtga tggctccgca ggcgaacaat
     1861 acgacaagcg cgatgaccga gctggacacg aatacgtcgg gcgacatatg ctccaataga
     1921 agagagctcg ccgctttgcc cgcggtgaac gagaggaacg cgacgcacca gggcatcagg
     1981 tagggtcgtt tgctttcgat gaggaagggg gccagcatga taaagaggaa caccagcttc
     2041 tgcgtgatgt tcagcaagac gaggtagagg aatatccacg tctcgttcac gaaggacgac
     2101 agccacaagg caagaagcac ggtgggtatg aggaacacct tgtacagctc gaggctccgc
     2161 ctgttccaga agtactggac gagcacgagg atggccgctc cgccgcatgc ggtgcccacg
     2221 gccgtgccca gctggatgat gagcgatacc gtcccgccgt cttgcagggg aatccattgg
     2281 tagtggatat gtccgaacac cacggcatag caagccagcg acagcatcat cgtcacgacg
     2341 aatactcgtc gaccgcgagg agcctggtcc gaggggacga tgagggatcg atcggggcag
     2401 ccgattcccg ccaccatgct ccgctccggg ctcgcgcagg catcctcgct cgggatgtcg
     2461 tcgcgatccg cacgcggcgc gcgcgaacgg gcgtattccc tgaaatagca gagcagcacc
     2521 atcgacacga tggggagcag cgataccgtg ccgtaagcgg catccacctt cagaaacgcg
     2581 atgaggctgt tcattgccgc aacgacgatc gttcccaggc ccagcacggc ggcgcaggcg
     2641 cgcgccccgt agggaaagag cgctttcatc cagagaaaca ggatgacgcc ctctccaagc
     2701 ccgaatacga tgcgcgcgat ggaggatacg atggggttgt cctcccatac gctttgctgc
     2761 aagacgacga acatggaagc cgacatcaac gctcctgcgc cgatgacgat cgcggcggga
     2821 acgtgggtgg gcgcgtccag cttgcgatac gcaagagcca gcatgatcag gagaaggaac
     2881 gatacgagcg acgcgacggc gtggtagtcg ccgctgatat tgacggttct gatcgtcgag
     2941 aggcggtcgc cttcgatggc gagcagcgcg catccgaagc cgatcgaagc gatcgcgatt
     3001 cccggcacct cgcgaatcgc cagcgcaagc ggcgacgatc ctcgttcgtc tgtcatgcgg
     3061 accccctttt actcacatcc ccatggcgat aaggctcgtt cgagcattgt cgtcagtata
     3121 gcaattcgcc gataccgcgg cggcgggcgg atggcgaaat accactctcg gtggtaggtc
     3181 tttcatgagc tcgatagtag ggtttctgac ctggttgaac agagttcgca tcagcggtgg
     3241 ggtgctcaac tcgcgctcga tctgcatcat ggttcgcacg gacgcttggt ccggcccgct
     3301 cgttggagtg cgggtgaaaa gggaaccggc agaggaggag ctatggcgaa caccacgcaa
     3361 aggcggtcgt tcggcaccat gagccgtcgc agcttcatga ggctcgcagg ggtgacgagc
     3421 gctgcgcttg cgctgacgtc ggcaacggcg ccggcggcgc ttgccgaaga gcacgacagc
     3481 ggaacggttg cgtcgtcgga cggcgtgcag cgtattcgca cgatgtgccg cgggtgcggc
     3541 aagatggaat gcggcgtgtg ggtgacggtg gagaacggcc gcgccatcaa gatcgagggg
     3601 gacgagagct cgttcgcgtc gtcggggaac agctgcagca agtcgcaggc ttcgctgcag
     3661 gcgtgctacc atcccgatcg gctcgcgtat cccatgaaac gcacgaatcc caagggcgac
     3721 gacgatccgg gttgggtgcg catcagctgg gacgaggctc tcgccgaagc cggcacgaag
     3781 ctcaacgaga tcaaggagca gcgcggcggg aactccatgt tctcgatgtg cggcaccagc
     3841 cgcatctact gcatggcgag cgcgctcggc atgcagggca ttctgaacac ggcgaacacc
     3901 catcaggcgt accagatttg caagggtccc cgccatgtgg ccaccggtat ggtgtcggct
     3961 cgtgcgtaca gctggatggc cacggtcgac cggccgagcg tgttcgtgca atggggcggc
     4021 gcttcggagc tgtccaacta cgatgactcg tgtcgcacga cggtcgatgc tgcggtcaag
     4081 gcggacaagc acatcatcgt cgatccgcgt cagacgaacc tcggcaaaga agccgacata
     4141 tggaatccgc tgcgtcccgg caccgacggc gcggtggggc tcggctggct caacgtgatc
     4201 atggagtaca acctgtacga cgagctttgg gtgaagcggt ggacgaacgg cccgttcctc
     4261 gtgtgcgagg atatcgagcc ttccggttgg cagcagatgg gtgccggcgg tccagaagaa
     4321 atcaaaacgc gcctgctcaa ggaatccgat gtgcaggaag acggcagtcc gaaacggttc
     4381 atggtctacg atcagttgaa ccagcggctt acgtatttcg atgcggatac gggatactgg
     4441 gagggcgagc agccgcgtac gctgacgggg aaggaggcgc ggcaaaagca cctcgcaccc
     4501 ggcgtgacgc aaggttgggt gcccgatcct accgggttcg atccggagat cgatcctcag
     4561 atattcgggc aggtggaagt caccctgaaa gacgcttcga cctcggtctg caaaacggtg
     4621 tggcaaacgt tctcggacta cgtagccgat ttcacgcccg agaaagtgga ggagatcacc
     4681 agcgtttcgg ctgatgcttt gcgcgaggcg gccatcacct atgcgacgcc tatcgatcca
     4741 tcgaccggat acggcaacgg cggcatccag tacatgctgg ccatcgagca tgcgtgcaac
     4801 tcggtccaga acagccgtat ctgcgatctc atcgtgggga tcacggggaa cttcgacacc
     4861 cccggcggca atcgcggcgc aacggccgcg actttcgacg aagagttcgc catgatgggc
     4921 agcggtctgc ccatggcgtc ggctgacttg tgggacaagg tgctgggcgt ggaggacatt
     4981 cctttgctca agcatcacgg catctgggcc gattcgacgg ccatttggga tgcgtgcaac
     5041 aacgaagggg cgccgtaccc gttgtacggc ggcgtctgcc agtcgggcga tgtgatgaac
     5101 atgtccaacg ctctttgggg ttgggaaggc ttgaagaagc tggacttcct gctggacatc
     5161 gacctgtggc atacgcccac gtcgcagctg gcggatatcc tgctgcctgc gcgccattgg
     5221 ctcgaggtgg attgccctcg ccgctcccaa gggtctggcg gcatggaggg aagccattgc
     5281 aagtgcgtgg agccgctcgg cgagagctgg ttcgacgtgg acatcatcat ccagctgtgc
     5341 aaggccatgg gcattccctg gagcgccgac cccgacgatc cgtggccgga ctccatcaag
     5401 gagcttgatg cggcatgcga gccgatgggt cttacctggg aggagtggaa gcaggagttt
     5461 cagaagaccg gtttccgcga ctgcaagaag gaataccccg acgactgggg cacttaccga
     5521 cgctacgaaa cgggccactg ccgctccgat ggcaagccgg gcttgcagac gcccacgctc
     5581 aagcaggaga tatggtcgac catcatcgag acgtaccatc ctgacggccg gtacaacctt
     5641 cccacgtatt ccgagccccc ggagagtccc gttgcgcagc ctgagcgggc tcaggagtac
     5701 ccctacatca tgacgacggg ccgtcgcatt cccgtgtact tccactccga gcaccggcag
     5761 ctgccgtggt gccgcgagct gtggccggtg ccgcgcgtag agatcaaccc gaaggatgcg
     5821 cttgagctcg gcatagagca gggcgattgg gtgtggatcg agacagagcg cggcaaggtg
     5881 cgacaagtgg cggatctcta tcacggcatc cgcccgggga ccatcaactg cgagcatcag
     5941 tggtggctgc ccgagttcca cggcgcgacg aagggtttcg acctcatcag catcaactgc
     6001 ctggtgaaca aggacatgcg cgatcctctg tgcggatctt cgtacgcgcg cgcttacaac
     6061 gtgaaggtgt acaaagccac gcccgagaac tcgccgttcg gcaatcccgt gccgtgcgac
     6121 gtcgacggga ccgagatgat cacgtcgccc gatgacccgc gtttgaagga atggctgccg
     6181 aactacgagg ggagggacta ggctatgact cagtatgcga ttgccaccga tctcaatcgg
     6241 tgcgtaggct gcctggcatg ctcggtgacc tgcaaagtgg tgaacgacgt tcctatcggg
     6301 tcgtattgga acaaggtgct gcgcatcggc ccgaatccga agttcgaagg cgccgagttc
     6361 cccgatgtgg agatgtactt cctcaccgtg caatgccagc actgcgccga ccccgagtgc
     6421 gtgaaggtgt gcccgaccgg tgcctcgcac aagctggagg acggaaccgt gcagatcgac
     6481 aagtccaagt gcatcggctg ccagttctgc gccatgtcgt gcccgtacgg ggtgcggtat
     6541 ttgaacgagg aggagcgcgt ggtggagaag tgcaccttgt gcgagcagaa gattgcccag
     6601 ggcgagctgc cgcagtgcgt cacgcaatgt ggcggccgtg cgcgctattt cggtgatctg
     6661 gagcaaggga tcgcaggttt cgagggcccg gctcccatcg atatcgcttc aaggaacgtc
     6721 gactacgatg cgaccgtcca gagtcgcgcg aagttggtcg acgagatcga gtcgtatgcc
     6781 gaaagcgatg tgtatcgcct gccgaacgtg gggaacaacc cgagcttcgt gtacatcctg
     6841 cgcaatcgta cgtggcaagg aggcggtgaa taatggaact gcagtggcct ttgattatct
     6901 ttaccaccct ggttgcctgg tcggcggggc tgttcgggac gcaggcgctg atggcggtgt
     6961 tcggcgtcgg caagaaggcg caggtgccgg cttgggtttg ctcggccgtc ttgctcgcgg
     7021 ccggcgggat cgcggtgttc ttccacctgg agcactggga gcggatcttc aacgggttcg
     7081 ggcacctgac gagcgggatc acgcaggagc tgatcgcgat cgtcgtgctg gcggtcgtcg
     7141 cggtcgcgta tctcgtgctc atgcgcaagt ccgacgacgg cgcgagcgtg cccaagtggc
     7201 tggcgtggct gtccgtcgcg ctgtcggtgg cgctcgtggc cgtcatggcg cactcgtaca
     7261 ccatggccgc gaggccggcg tgggactcgg tcctctggat cctctacgtc ctcggcaacg
     7321 cctgcgtgtt ggggccggcg acgttcctcc tcctctccgc cttggctgca gggggacccg
     7381 gggaccagcc cgccgagcgc gcagccggcg ccggggcccc cgcgggtctc gcggccctcg
     7441 ccggcgccgc cctcaacgcc ctcgcggccc tcgccttcgc cgccttcctc cagctctccg
     7501 ccggctcctt cgccgacgtc ggcctctact tcgacccgac gcaccccacc agggccatgg
     7561 ccgacgccgc ggccaccgtg ggcgcgcagg ccccgctcct gtggctggga gtggtggccg
     7621 tcggcgcgat cgtccccctg gccgccgcct tcgtgggcag gcgcaccggc aactggaagc
     7681 tctgggcccc ggtcgccatc gccgccgccc tcgtcggagc cgtctgcatg cgcgtcgcct
     7741 tctacaacct ggggctctcg gtcttcatgt tctactagac tagtcgactc acactggctc
     7801 accttcgggt gggcctttct gcgtttatat actagagaga gaatataaaa agccagatta
     7861 ttaatccggc ttttttatta tttaattcct gttaccgctg gttgccacga cgccctcgaa
     7921 gtctccttcg agggcgttct gtcgtttgca agaagggtct ggagaggcaa atcgcgctcg
     7981 aaggggcttc gaggccgggg taggtcggga gggtggaatc cggatgtttc acgtgaaaca
     8041 tccgttcttt tacggccggt gtttcgcgtg aaacattttg cggaaaggac tccttccctt
     8101 cccgtgcgcc accccaacgc cccgctccta gcgctcgtac acctggtcgc agttgagcag
     8161 atccttcatg gagtgccaga tctggtgcgg gttgcccggc gggttcttga gcggcttcat
     8221 cggatagcgc tcctgcgcgc ccgacagcag atcgacggcg aacacttcgt cggacagctc
     8281 gagcgcgcct tccgggcaga tgtcggtgca gcagcggcac ttcacgcaat cgaccggacg
     8341 atgcgtcacg ccgatcgagc cgtcctcacc cgcgtacttc gcgatggccc cggtggggca
     8401 gaacgtggcg cacatctggc acgacgagca cttctcggga tcgatgatca catggcccca
     8461 caagcgcgtg tccatgagca catcctgcgg ctgccccaac gaatcgagcg cggccagcaa
     8521 acgttcgcgg cggtcgggaa tgaagtgagg cagcgtgccg tccttgccca ccttcacgaa
     8581 cctcggctcc cgccgctcct ccacacccaa tgcgtccttg acggcgtagt cagccgtcac
     8641 cgccgcaacg ttcttagcct cgtccttcat gctggagaag aagctccgac ggctcgcgtc
     8701 gtagccctcg tcgcccgccc ggcgcacgga tgagggaagc ttttccgcca ggtcgatgcg
     8761 catatcgttg ttccaggttg caagcagcgc gcttgccgtg tcgcacacaa cccgcgccat
     8821 ctcgaaaccc gagacatgct cgcattgcgc gcagcgcgcc tgcacgaggc tcacgcggcg
     8881 cacgcccaac gccgccagcg tgaccagcag gctctcctcc acgcgcccca ggcacgtgac
     8941 gcccaccacc ttctcggggt cgtacaaccc gtcggccgcg gcaagcagct gatcgcaggc
     9001 gataaccacc tgtccgtcgg ccacgtcagc cgccgcgcgg catgcttgca gaagctcggc
     9061 atcgttcgga cgatgcgctt caagcgcgca ggtgggacac acggtggcgc aggttccgca
     9121 cccgatgcac cgctcgggtt cgatgaccag ctcgttgtcg tcataggaga tgcaaccgct
     9181 cgtgcacacg tcggcgcact tcatgcacgt tgcgttgcga ttgcgcacaa tagcgcaacg
     9241 accctgatga accgtaatgt cggcgctctg aagttgttcg agcagaccga ggaaattcgt
     9301 cacgtgagcc atgacttcac cctttctcgt ctaccccctt gcaccgcaca gtataaccac
     9361 tttcgccgtc tcacgcgaga aaaaacgcgc ttgacacctt cgacaggcct ccctatcatg
     9421 tggccttggg gagtccgccg gcacgctgat cgcgtgcgaa gcggacattg tttgcaaaag
     9481 tgggaacctc aggggtgagg tttcgcggtg ctgtgcgccg taagtgcaaa caatgtctga
     9541 cgaggggcgg aacatgactc acgattccac gaaccagcat ctttctcttg agcccgacta
     9601 cgacgggttc gatccacttg aagatttcga agacgatgcg accgaggtcg aaggccgtgc
     9661 cgacgagttc gtcgccacgg tgagcgccgc cgaagacgcg ctcgctcacg acgcgatggc
     9721 acccgccacg cccgccatct gcgaaaagcc cgctgaagag cgcatcgccg acttgttgga
     9781 ggccatggcg ccgcgccgca cggtgctgct gggcatcctg gccgcctgcg ccgagccgca
     9841 gccggcatcc gccgtgaacg ctcgcgtcga cgagctgcag gagaacaact tctcggtgta
     9901 cagcgcggcc aacctgtgct ccttgctgga gaaggcgggc gccatcgagc gcgtcacggc
     9961 cgagggcgaa cccgcggagg acatcgaaat cgagccacag accgtcgtcg tcgacggcgt
    10021 cgaatacctg gaagcccgcg aacccgtcga gatctactgg cacatcaccg agccgggccg
    10081 cgcggcgctt gaggccgaca aaccgctcga gcgcctgcgc gccctcctgg acgaagatgc
    10141 cgcctacgcg cccatctacc agcgcatcct ccgcttgtgc acagcagacg gcggcgccac
    10201 gacacccgcc atcaacaacg cggtcgacca caacccgctg gtgcagaagc cgcgcttcta
    10261 cgcgccccac ttcgtcgacc gcctggagaa atgcgacgcg ctggcctgga agaaggcgtg
    10321 gtgcatcacc gacatcggac gcgccggcct cgatatgctg gcagacgtcg tagacgagaa
    10381 cgcgcccgcc acccagcccg aaacccccgc aacccctgat cccgccgcat ccaaggagga
    10441 ctaaccatga ccgaagcaac cgcgcccgca accgagaccc tcgccgacga agcgaccatg
    10501 caggagctcg tcaaactgaa cgagcagcgc gccgccacgt acggcctgct gtcgcgcctg
    10561 taccgcgtgg aggtggacca gccccttctc gacgagctgc gcagcatgcg cttccccgcc
    10621 aagacgggca acgagaacgt ggacgagggc taccgcctgc tggccacgta cctgagcaac
    10681 acctgggaca acagcgtcac cgacctcgcc atcgactacg tgcgcgtgtt catcggacac
    10741 ggcatcgacg cgttctcggc cgcctacccg ttcgaatcgg tgtacacctc cgagaagcgc
    10801 ctgctcatgc aggaagcacg cgacgaggtg ctggccatct accgcagcgc aggcctcgac
    10861 aagcaggaca catggaaaga gggcgaggat catatcgcgc tcgagctgga attcgagcag
    10921 atcatggcca cgcgcacggt cgaagcgctg cgcaaaggcg acgaagacga ggccgtcgcg
    10981 ctgctgacca cgcagaagaa ctttctcgaa gaccatctgg cggcatgggc tcccatgatg
    11041 acctccgaca tgaagcgatt cgcgcaaacc gacttctacc ggggcttggc gtacttgacg
    11101 gacgggttcc tccaaaccga ccaaacgttc ctcgacgacg tgctggccga agactaactg
    11161 cacaggatcg aaagaaggag cgatggacaa caagcaggag aaaacctacg ccggcatgac
    11221 gcgacgcacg ctgtgcctgg gcgcaggcgg cgctgcggtc atgctaggtc taggcggatt
    11281 gaagtacgcg ggatcgcaac cgctcgtgcg cccgccggga ggccaggacg aggaacggct
    11341 catctcggcc tgcatccgct gcgagaagtg ctacgagatc tgcccgcgcg acgtcatcgc
    11401 gcccgcccac ctcgaggacg gtgtgctgaa catgcgcacc cccacgttcg acttctcggc
    11461 caactggtgc gactggtgcg ccgaagagca cgacggcacc ccgttgtgcg tgagcgcctg
    11521 ccccaccgaa gcgctcaagc tgcccgacga cgccacggcc gagaacacca tcatcggcaa
    11581 agccgttatc aacacggact ggtgcttggc gtacaagctg atcggatgcc ggttttgcta
    11641 cgacgcctgt ccctacgagg ccatggagct cgacgagaac aaccgccccg tcgtgatcga
    11701 ggacaagtgc aacggctgcg gcgcgtgcga gagcgtgtgc gtctcgctgc agaacggctc
    11761 catctccgaa ggagccaccg cgcgcgccat cgtcgtccac ccgcttgacg aagttgaagg
    11821 ataaggtgcc ctcatgaaga agaactccaa gaagctgcgc accctcgtcg cactggccat
    11881 cgtggccgtc gtcgccgtcg gcttcgtcac gaacgtcggg atcggcacca tgtcggcacc
    11941 gggcatctgg gacatctcca tcctatgccc gctgggcgcc ttgggaacga tgctcgcctc
    12001 caagatgatg gtgccgcgcg ccgtaatctc gctgatcgtg atggtgctgc tcatcgttct
    12061 gttcgcccgc gcgttctgcg gatggatttg ccccgtgccg ctcgtgcaga agttgcgcgg
    12121 cttgttcgcg aagaagcaac ccgtcgaacg cggcgacgat gccgatcctg ccgcggcgcc
    12181 gctcacgaaa gtcgagatcg acgcgctggg cgtttcatgc tcgaaggggt cgaagaatgg
    12241 cagcggatgc gcgtcctgcg cctccaagcg cggagccgcg ctcgatgctc gccacttcgt
    12301 gctgggcggc gcgctggcgt ccaccttcat ctttggattc ccggtgttct gcctcgtctg
    12361 ccccatcgga ctcacgttcg ccaccatcct gctggtcatc aacctgttct cacacggcga
    12421 tgtcacctgg tcgctcatcg tggtgcccgc gctgcttatc gccgaggtgc tgctgttcaa
    12481 gaagtggtgc cataagctgt gcccgctttc ggcgttcatg agcctgatcg cgaaggcgaa
    12541 caagaccttc gtgcccacga tcgacgatgc gaagtgcctg gaaacggcga agggcggcgc
    12601 gtgcggggcc tgcggccgcg tctgcgagga gggcatcgac ccgcgccacc cgcagctttc
    12661 gcaggcggca tggagcgagt gcaccaagtg ccgcgcctgc gtggacgcat gccctgcgaa
    12721 cgcgatcacc atgccgctgc tgccgcggcg aacgccgtct accgcagaac ctgtcgccgt
    12781 gccaacgaac gccgagaaga tcgacgcgga gaatgcgcgc cccgttccgt aaagcccctc
    12841 ctgcgcgacc cggctcgcag ctgcagcggt ctggtcgcgc atccggaatc gggccgagcc
    12901 cgaccccggg caaaaggcgg cttggcaagg gctttggggg acgagccgcc ttttgcccgg
    12961 gagcgacatc cccgctcccc ctccctgcga aaagagggat gccgcttccg gccttcatct
    13021 gacaaccacg acgtgggagg acgaaaaccc atggcatacg aaacgacaag cgaatcgacc
    13081 gaggccctgc tcgtcaccgg aggcgtcggc accggcaaaa cgcaacgtct cgtcgagcgc
    13141 gctgccgcgc tgctggccga aaacgccgcc gcgggcaacg tgctcgtgct gtgcgcgacg
    13201 ccccaggccg cacgcctgtt ctcggagcgg ttgacagccc gcgtcggtgc caagcgcgct
    13261 gccggcgcaa ccgtcgccac cgcacgcgcc ttagcgctcg acgtgctggc cgacgacggc
    13321 gcgcgccgct ggtcggggcg cgagccgcga ctactgaccg ccttcgaaga gacgttcctc
    13381 atggaggaca tgaaggtgag cgggctgcgg ccgaagcggc tgcgcgagat gctcaagttc
    13441 ttctaccgct cgtggaccga gctggcggac gacgatcccg attggctgct accgggcgag
    13501 gaggccgatg tccatacgct gttgaaagag aacctggtct tcatgcgcgc cgtcatagaa
    13561 cccgaagcgg cgaaccttgc tgcacgctac ctccgagaac acgacgaagc gcgggcggcg
    13621 cattcctacg cgcacgtgct gatggacgac tacgcctgcg caagccgtgc ctcgcagacg
    13681 ctggcgaatc tcgtcgcaac ctgctcgatc accgtggcgg gcgacgcgga aacctgcatc
    13741 gagacatacg attcgtaccc ctatgccgcc ggaatcgacg aattcctcgc aacgcatccc
    13801 gatgctgctt gcgagcacct ggatgccgac gcgcccgatg ccgtcgaacg ggtcgctctg
    13861 gcaagcgaca cgcccgaagc cgagttcgaa caggtggcgc gcacggtggc cgaagccgtc
    13921 gccgcaggaa cgcccgcacg gcagatcgtc gtggccgtac cgaacagcgt gtggtcgcgc
    13981 aacatcgcaa ccgctttgca cgcacgaggc gtgccaaccg aagcgctcgc catctcgcag
    14041 cccctgcgcg gcgacgtgcg ggacaacgcc cgctgcacgc ctgcgcgcat cgccacggcc
    14101 ctcaacctcg tggcgaaccc agacgacacc gcggcttggc ggtgctggtg tggattcggc
    14161 gactacctgg tgaacagcgc ggctttcgcc agcctgcgga cttgcgcgaa ggagcgcagt
    14221 atcggcctcg tggacgcttt ggagatgcta agcgaaaacg actgcgagca cgtagtgggc
    14281 gcgcaacgcg tggcgaccgc ctacaaggac ggtcgcgcgc tcatcgagca ggtacgagga
    14341 ttgcggggca cggcactgct ggacgagctg acgcgcatcg tcaccgacgg agcggaatcc
    14401 caagcgcccg ccgttctgcg cgagctctgc ctcgagcacg gcaacgcagc cgatgaaaac
    14461 ccggaaagcc tttacgatgc cgatgctgcg gcgatggcgc ggcgcatggc cgagcgcctg
    14521 ctcgcgccca cgctggaggt tgccgacgcg gtagccgtcg tcccctacga cttcgcctgc
    14581 ggcttctcgc ccgacctgct gatcgtcgcc ggcttcgtga acggtttcat cccctcccgc
    14641 gactatttcg acgccacggt gaccccgccc gagcgccagg agaagatgcg cgccgtcgac
    14701 gaacgacgcg tgcgcgcgct cacaggaaag gccggccgcg cggtggcgct gtcgtacttc
    14761 acgtccaccg acctcgaaag cgccggcaag ctgaagctca agatcagtcg catccgcctc
    14821 aagaacggta tccgcacctg cactatagag cccagcctat tcctggagca aatcgcagcg
    14881 caggagtagt aaaaaaagga tcaattttga actctctccc aaagttgatc ccttaacgat
    14941 ccctgcagga gaaggccatc ctgacggatg gcctttttga agctttctag aatcgggggc
    15001 ccccgggggg attctagaac aatggaagtc ctcgtctcgc cctcgaatta gcccgcttaa
    15061 tgagcgggct ttttttgaat taattctcgc gagctggcac gacaggtttc ccgactggaa
    15121 agcgggcagt gagcgcaacg caattaatgt aagttagcgc gaattgcaag ctggccgacg
    15181 cgctgggcta cgtcttgctg gcgttcggga gcagaagagc atacatctgg aagcaaagcc
    15241 aggaaagcgg cctatggagc tgtgcggcag cgctcagtag gcaatttttc aaaatattgt
    15301 taagcctttt ctgagcatgg tatttttcat ggtattacca attagcagga aaataagcca
    15361 ttgaatataa aagataaaaa tgtcttgttt acaatagagt ggggggggtc agcctgccgc
    15421 cttgggccgg gtgatgtcgt acttgcccgc cgcgaactcg gttaccgtcc agcccagcgc
    15481 gaccagctcc ggcaacgcct cgcgcacccg ctggcggcgc ttgcgcatgg tcgaaccact
    15541 ggcctctgac ggccagacat agccgcacaa ggtatctatg gaagccttgc cggttttgcc
    15601 ggggtcgatc cagccacaca gccgctggtg cagcaggcgg gcggtttcgc tgtccagcgc
    15661 ccgcacctcg tccatgctga tgcgcacatg ctggccgcca cccatgacgg cctgcgcgat
    15721 caaggggttc agggccacgt acaggcgccc gtccgcctcg tcgctggcgt actccgacag
    15781 cagccgaaac ccctgccgct tgcggccatt ctgggcgatg atggatacct tccaaaggcg
    15841 ctcgatgcag tcctgtatgt gcttgagcgc cccaccacta tcgacctctg ccccgatttc
    15901 ctttgccagc gcccgatagc tacctttgac caccatggca tcagcggtga cggcctccca
    15961 cttgggttcc aggaacagcc ggagctgccg tccgccttcg gtcttgggtt ccgggccaag
    16021 cactaggcca ttaggcccag ccatggccac cagcccttgc aggatgcgca gatcatcagc
    16081 gcccagcggc tccgggccgc tgaactcgat ccgcttgccg tcgccgtagt catacgtcac
    16141 gtccagcttg ctgcgcttgc gctcgccccg cttgagggca cggaacaggc cgggggccag
    16201 acagtgcgcc gggtcgtgtc ggacgtggct gaggctgtgc ttgttcttag gcttcaccac
    16261 ggggcacccc cttgctcttg cgctgcctct ccagcacggc gggcttgagc accccgccgt
    16321 catgccgcct gaaccaccga tcagcgaacg gtgcgccata gttggccttg ctcacaccga
    16381 agcggacgaa gaaccggcgc tggtcgtcgt ccacacccca ttcctcggcc tcggcgctgg
    16441 tcatgctcga caggtaggac tgccagcgga tgttatcgac cagtaccgag ctgccccggc
    16501 tggcctgctg ctggtcgcct gcgcccatca tggccgcgcc cttgctggca tggtgcagga
    16561 acacgataga gcacccggta tcggcggcga tggcctccat gcgaccgatg acctgggcca
    16621 tggggccgct ggcgttttct tcctcgatgt ggaaccggcg cagcgtgtcc agcaccatca
    16681 ggcggcggcc ctcggcggcg cgcttgaggc cgtcgaacca ctccggggcc atgatgttgg
    16741 gcaggctgcc gatcagcggc tggatcagca ggccgtcagc cacggcttgc cgttcctcgg
    16801 cgctgaggtg cgccccaagg gcgtgcaggc ggtgatgaat ggcggtgggc gggtcttcgg
    16861 cgggcaggta gatcaccggg ccggtgggca gttcgcccac ctccagcaga tccggcccgc
    16921 ctgcaatctg tgcggccagt tgcagggcca gcatggattt accggcacca ccgggcgaca
    16981 ccagcgcccc gaccgtaccg gccaccatgt tgggcaaaac gtagtccagc ggtggcggcg
    17041 ctgctgcgaa cgcctccaga atattgatag gcttatgggt agccattgat tgcctccttt
    17101 gcaggcagtt ggtggttagg cgctggcggg gtcactaccc ccgccctgcg ccgctctgag
    17161 ttcttccagg cactcgcgca gcgcctcgta ttcgtcgtcg gtcagccaga acttgcgctg
    17221 acgcatccct ttggccttca tgcgctcggc atatcgcgct tggcgtacag cgtcagggct
    17281 ggccagcagg tcgccggtct gcttgtcctt ttggtctttc atatcagtca ccgagaaact
    17341 tgccggggcc gaaaggcttg tcttcgcgga acaaggacaa ggtgcagccg tcaaggttaa
    17401 ggctggccat atcagcgact gaaaagcggc cagcctcggc cttgtttgac gtataaccaa
    17461 agccaccggg caaccaatag cccttgtcac ttttgatcag gtagaccgac cctgaagcgc
    17521 ttttttcgta ttccataaaa cccccttctg tgcgtgagta ctcatagtat aacaggcgtg
    17581 agtaccaacg caagcactac atgctgaaat ctggcccgcc cctgtccatg cctcgctggc
    17641 ggggtgccgg tgcccgtgcc agctcggccc gcgcaagctg gacgctgggc agacccatga
    17701 ccttgctgac ggtgcgctcg atgtaatccg cttcgtggcc gggcttgcgc tctgccagcg
    17761 ctgggctggc ctcggccatg gccttgccga tttcctcggc actgcggccc cggctggcca
    17821 gcttctgcgc ggcgataaag tcgcacttgc tgaggtcatc accgaagcgc ttgaccagcc
    17881 cggccatctc gctgcggtac tcgtccagcg ccgtgcgccg gtggcggcta agctgccgct
    17941 cggacagttc gaggctggcc agcctgcggg ccttctcctg ctgccgctgg gcctgctcga
    18001 tctgctggcc agcctgctgc accagcgccg ggccagcggt ggcggtcttg cccttggatt
    18061 cacgcagcag cacccacggc tgataaccgg cgcgggtggt gtgcttgtcc ttgcggttgg
    18121 tgaagcccgc caagcggcca tagtggcggc tgtcggcgct ggccgggtcg gcgtcgtact
    18181 cgctggccag cgtccgggca atctgccccc gaagttcacc gcctgcggcg tcggccacct
    18241 tgacccatgc ctgatagttc ttcgggctgg tttccactac cagggcaggc tcccggccct
    18301 cggctttcat gtcatccagg tcaaactcgc tgaggtcgtc caccagcacc agaccatgcc
    18361 gctcctgctc ggcgggcctg atatacacgt cattgccctg ggcattcatc cgcttgagcc
    18421 atggcgtgtt ctggagcact tcggcggctg accattcccg gttcatcatc tggccggtgg
    18481 tggcgtccct gacgccgata tcgaagcgct cacagcccat ggccttgagc tgtcggccta
    18541 tggcctgcaa agtcctgtcg ttcttcatcg ggccaccaag cgcagccaga tcgagccgtc
    18601 ctcggttgtc agtggcgtca ggtcgagcaa gagcaacgat gcgatcagca gcaccaccgt
    18661 aggcatcatg gaagccagca tcacggttag ccatagcttc cagtgccacc cccgcgacgc
    18721 gctccgggcg ctctgcgcgg cgctgctcac ctcggcggct acctcccgca actctttggc
    18781 cagctccacc catgccgccc ctgtctggcg ctgggctttc agccactccg ccgcctgcgc
    18841 ctcgctggcc tgctgggtct ggctcatgac ctgccgggct tcgtcggcca gtgtcgccat
    18901 gctctgggcc agcggttcga tctgctccgc taactcgttg atgcctctgg atttcttcac
    18961 tctgtcgatt gcgttcatgg tctattgcct cccggtattc ctgtaagtcg atgatctggg
    19021 cgttggcggt gtcgatgttc agggccacgt ctgcccggtc ggtgcggatg ccccggcctt
    19081 ccatctccac cacgttcggc cccaggtgaa caccgggcag gcgctcgatg ccctgcgcct
    19141 caagtgttct gtggtcaatg cgggcgtcgt ggcctgcccg ctctaatgcc cggttggcat
    19201 ggtcggccca tgcctcgcgg gtctgctcaa gccatgcctt gggcttgagc gcttcggtct
    19261 tctgtgcccc gcccttctcc ggggtcttgc cgttgtaccg cttgaaccac tgagcggcgg
    19321 gccgctcgat gccgtcattg atccgctcgg agatcatcag gtggcagtgc gggttctcgc
    19381 cgccaccggc atggatggcc agcgtatacg gcaggcgctc ggcaccggtc aggtgctggg
    19441 cgaactcgga cgccagcgcc ttctgctggt cgagggtcag ctcgaccggc agggcaaatt
    19501 cgacctcctt gaacagccgc ccattggcgc gttcatacag gtcggcagca tcccagtagt
    19561 cggcgggccg ctcgacgaac tccggcatgt gcccggattc ggcgtgcaag acttcatcca
    19621 tgtcgcgggc atacttgcct tcgcgctgga tgaagtcggc cttggccctg gccgattggc
    19681 cgcccgacct gctgccggtt ttcgccgtaa ggtgataaat cgccatgctg cctcgctgtt
    19741 gcttttgctt ttcggctcca tgcaatggcc ctcggagagc gcaccgcccg aagggtggcc
    19801 gttaggccag tttctcgaag agaaaccggt aagtgcgccc tcccctacaa agtagggtcg
    19861 ggattgccgc cgctgtgcct ccatgatagc ctacgagaca gcacattaac aatggggtgt
    19921 caagatggtt aaggggagca acaaggcggc ggatcggctg gccaagctcg aagaacaacg
    19981 agcgcgaatc aatgccgaaa ttcagcgggt gcgggcaagg gaacagcagc aagagcgcaa
    20041 gaacgaaaca aggcgcaagg tgctggtggg ggccatgatt ttggccaagg tgaacagcag
    20101 cgagtggccg gaggatcggc tcatggcggc aatggatgcg taccttgaac gcgaccacga
    20161 ccgcgccttg ttcggtctgc cgccacgcca gaaggatgag ccgggctgaa tgatcgaccg
    20221 agacaggccc tgcggggctg cacacgcgcc cccacccttc gggtaggggg aaaggccgct
    20281 aaagcggcta aaagcgctcc agcgtatttc tgcggggttt ggtgtggggt ttagcgggct
    20341 ttgcccgcct ttccccctgc cgcgcagcgg tggggcggtg tgtagcctag cgcagcgaat
    20401 agaccagcta tccggcctct ggccgggcat attgggcaag ggcagcagcg ccccacaagg
    20461 gcgctgataa ccgcgcctag tggattattc ttagataatc atggatggat ttttccaaca
    20521 ccccgccagc ccccgcccct gctgggtttg caggtttggg ggcgtgacag ttattgcagg
    20581 ggttcgtgac agttattgca ggggggcgtg acagttattg caggggttcg tgacagttag
    20641 tacgggagtg acgggcactg gctggcaatg tctagcaacg gcaggcattt cggctgaggg
    20701 taaaagaact ttccgctaag cgatagactg tatgtaaaca cagtattgca aggacgcgga
    20761 acatgcctca tgtggcggcc ctcgcgagaa ttaattcaga taaaaaaaat ccttagcttt
    20821 cgctaaggat gatttctagc gatgaccctg ctgattggtt cgctgaccgt agactggttg
    20881 gcttggtttc atcagccatc cgcttgccct catctgttac gccggcggta gccggccagc
    20941 ctcgcagagc aggattcccg ttgagcaccg ccaggtgcga ataagggaca gtgaagaagg
    21001 aacacccgct cgcgggtggg cctacttcac ctatcctgcc cggctgacgc cgttggatac
    21061 accaaggaaa gtctacacga accctttggc aaaatcctgt atatcgtgcg acatcgcgcg
    21121 cgcgcgcgcg gatgctagcg gcgcgccaaa aaaaagcccg ctcatta
//
