LOCUS       sequence-463812-       21923 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Eggerthella lenta A2 Dadh with the accessory genes from 
            the cadh gene cluster in Gordonibacter urolithinfaciens DSM 27213.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 21923)
  AUTHORS   Dong X, Bae M, Le C, Aguilar Ramos MA, Balskus EP
  TITLE     Enantiocomplementary Gut Bacterial Enzymes Metabolize Dietary 
            Polyphenols.
  JOURNAL   J Am Chem Soc. 2025 Mar 5;147(9):7231-7244. doi: 
            10.1021/jacs.4c09892. Epub 2025 Feb 24.
  PUBMED    39993729
REFERENCE   2  (bases 1 to 21923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.4c09892"; journalName: "J Am Chem Soc"; date: 
            "2025-03-5- 5"; volume: "147"; issue: "9"; pages: "7231-7244"
COMMENT     Sequence Label: pXD80ENSF30-DadhABC(cHis8)RS-Accs(Cadh)(ElA2)
FEATURES             Location/Qualifiers
     source          1..21923
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(19..813)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     primer_bind     1300..1318
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    1475..1494
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     CDS             complement(3005..3028)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     terminator      8552..8579
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      15706..15733
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(16165..17016)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(17003..17842)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(18353..19324)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELSERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     oriT            20563..20650
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     rep_origin      20992..21386
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     terminator      complement(21549..21592)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     oriT            21688..21797
                     /note="incP origin of transfer"
ORIGIN
        1 ggcgggctgg ccggccttct aaaacaattc atccagtaaa atataatatt ttattttctc
       61 ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg acatactgtt cttccccgat
      121 atcctccctg atcgaccgga cgcagaaggc aatgtcatac cacttgtccg ccctgccgct
      181 tctcccaaga tcaataaagc cacttacttt gccatctttc acaaagatgt tgctgtctcc
      241 caggtcgccg tgggaaaaga caagttcctc ttcgggcttt tccgtcttta aaaaatcata
      301 cagctcgcgc ggatctttaa atggagtgtc ttcttcccag ttttcgcaat ccacatcggc
      361 cagatcgtta ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc tattcgtata
      421 gggacaatcc gatatgtcga tggagtgaaa gagcctgatg cactccgcat acagctcgat
      481 aatcttttca gggctttgtt catcttcata ctcttccgag caaaggacgc catcggcctc
      541 actcatgagc agattgctcc agccatcatg ccgttcaaag tgcaggacct ttggaacagg
      601 cagctttcct tccagccata gcatcatgtc cttttcccgt tccacatcat aggtggtccc
      661 tttataccgg ctgtccgtca tttttaaata taggttttca ttttctccca ccagcttata
      721 taccttagca ggagacattc cttccgtatc ttttacgcag cggtattttt cgatcagttt
      781 tttcaattcc ggtgatattc tcattttagc catgatgttt aactttgttt tagggcgact
      841 gccctgctgc gtaacatcgt tgctgctcca taacatcaaa catcgaccca cggcgtaacg
      901 cgcttgctgc ttggatgccc gaggcataga ctgtacccca aaaaaacagt cataacaagc
      961 catgaaaacc gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt
     1021 gcgtgagcgc atacgctact tgcattacag cttacgaacc gaacaggctt atgtccactg
     1081 ggttcgtgcc ttcatccgtt tccacggtgt gcgtcacccg gcaaccttgg gcagcagcga
     1141 agtcgaggca tttctgtcct ggctggcgaa cgagcgcaag gtttcggtct ccacgcattc
     1201 gaaaccggta tcccgccgcc gccgccggat accggtttcg aattgagatt gccacctgac
     1261 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     1321 tttcgtcttc aaggcgaaaa aaccccgccg aagcggggtt ttttgcgaaa tataatgacc
     1381 ctcttgataa cccaagaggg cattttttag gaacggttat ttgattatta atccggcggc
     1441 aactgaaacg attcggatcc tgtattacta ttctgaattc gcggccgctt ctagatttag
     1501 tacatcagga ccactgggta gcccagctgg tagatcagca cgcgaaacat cacggaggtg
     1561 gccagcgcgc cgatggcggc gacgaccatg agcgtcttgg aggcgcccac cttcttcttc
     1621 gccacgaagg cgcaggccgc agccacgatc acggagacga tggagcccca gaacatgggc
     1681 gctcccgcgc cggacaccat gacggtcatc aggctgtcga cgtgcgtggg ggccgtggtc
     1741 atcgatgtcg ggtcggcgta gtagccgaat tgcgcgatct tcgccatggc gcaggcgatg
     1801 acgaacacca cgtcggccac gatctgcacg agggccgcga tgaacgtcag ctggatgccc
     1861 gtcgcctcga cggcctcgtc cttcttcagg atggagatca gccagacggc cgcggcgccc
     1921 aggaggcacg cgttgcccag atagaacgcc acgagcgcca ggccccacgc gggacgggcc
     1981 ggcatgaggt aggagtgcgc ggtggcgacc atcatgagca cggcaaccgc gagggtcgcc
     2041 caggccagcg ccttgggaac gggcttgccg cctcgaagga ccacgaacca tgcgacgatg
     2101 acgatggcca gcgccacgca tccgatgagc tcctgggtga tgcccgacgt gatgtgcccg
     2161 aagccgttga agatgcgctc ccagtgctcg aggtgcagga aggctccgat gccgcctacc
     2221 accagcgaga cggcggacgt gatgagcgcg gtcatctgca ggttcctgcc ctggcccttg
     2281 agcgccagga tggacatgcc gcccagcgtg ccgcaggtca agcatacgaa cagcgtgaag
     2341 atgacgagag gccactgcgt ttccattact catcacctcc ttcccagtga tggtcgcgta
     2401 ggatgtagga gtacgacggc ttgttgccca cgtcgggcag cttgtgcacg tcggcgtcgg
     2461 tgaacggctc caggaactcc accatcttgc ggcgcttgcc cagcgggtcc tcggagccct
     2521 cgaacgactc gtagccggcc tcggtgtcgc cgatccagcg ggcgttgccg ccgcactggc
     2581 tgacgcactg gggcagctcg ccctgcgaca gcttctgctc gcacagcgtg cacttctcga
     2641 cgaccttctc ctcctcgttg aggtagcgga cgccgtaggg gcaggccatg acgcagaact
     2701 ggcagccgat gcacttctcc ttgtcgatct ggatggagcc gtcctcggcc acgtgcgacg
     2761 cctcggtggg gcacaccttc acgcactcgg ggttctcgca gtgctggcag ctgatgggca
     2821 ggaagtacat gtacacgtcg gggtactgcc ctgatccgcc cgggatgggg ttcggcccca
     2881 cgcgcaccac tttgttccag tagctgccga tgggcacgtt gttgaccgcc ttgcaggcga
     2941 ccgagcacgc caggcagccc acgcaccggt tgaggtcggt gatgatagcc ttcgtcatgg
     3001 ttcagtgatg gtgatggtga tggtgatgct cgagtgcggc ctccctccct tcgtaggtag
     3061 gcagccattc cttcagacgg gggtcgtcgg aggtatggat gatgggcgtg ccggtggagt
     3121 cgcacggcac ggggttgttg aacggcgagt tctcgggcgt ggccttgtag atcttcacct
     3181 ggtaggcgcg caggttggac gtgccggagt gcgggtcctg ggcgtagtgg tcgatcagct
     3241 ggttcacctg ggagaactgc cagccgtggc cggcgtcctt cacctcgggg taccaccacg
     3301 tgtgctcgag gttgatgacg tcttccttca cgccgtagta caggtcagcc acttcgcgga
     3361 tcttgcccca ctcggtttcg atccacaccc agtcgccctg ctcgatgccg tactcggcgg
     3421 ccgtcttcgg gttgatctcc acgcgcggca cgggccacag ctcgcggcac cagggaagct
     3481 ggcgatgctc ggagtggaag tacaccggga tgcgacggcc ggtggtggcg gtcagcgggt
     3541 actccttgat gcggtcgccg tccttcgggc cgtgaggcgg ctcggtgtag gtgggaagcc
     3601 tccactcggg atggtcggga tggtgcgatt ccatgacggt ggaccagatc tcctgcttca
     3661 tcgtcggggt gaaccagccc ggcttccagt cgccgatgcc cttgccggcc gtgtagtcga
     3721 ggcggcccca cacgcggtcg cgtgcgcgca tcgcgccggt ctggtagcgg cggtacgtgc
     3781 cccagttgcg cggctcgatg tccttcgcct gccaccagcc gtgctcctgg aaggccttga
     3841 cgtactcgtc ccacgtcttg tacttgggcg tgacctcggc catcggaacg ccgtggcgct
     3901 cgaacgtggg cttgccgttc tcgacgtgcc acgtggtgta ggtcagctcg tcgtcggtga
     3961 gcagcttgat ggagtcgtcc agctgccagt tgatgtcggg ccactcgttg cccggctcgt
     4021 cggtccactt ccagttcatg cggcgggcga ggtccatgac gatctcggga tcgtacttgg
     4081 cttccgcggg cggctgcacg cacttgaccg tggcgcccat ggcgccggcg gaaccttggc
     4141 tggcgcgcgg cgagttgagc tcgatccaat gggccaccgg caggatgatg tcggcggtgt
     4201 cgttctgcgg cgtgtgccac aggttgaggt ccacgtagaa gtcaagcgag cacagggcct
     4261 cccaggcgcg cgtggtgttc gtctggctca tgaagttgcc ggactcgttc cagagggcgc
     4321 gcaccgggta ggggtcgccc gtgatgacgg cgtcccacag cgaatggctg tcgcaccagt
     4381 actgccacca gcccagaagc gggaacttgt cgatgccgat ctgcttgcgg ttgacttcct
     4441 ccggcggggt ggtggcgccg ggggcccagg cgctgaagcc ctggaggtcg ccgtcgatgg
     4501 ggacgatggt cgggccgcgg ttgccgcccg gggtgtccat gttgccggtg atgcccacga
     4561 ggttgtcgaa ggcgcggcag ttctggatgg cgctgcagaa gtgctcgacc gccagcatgt
     4621 actggatgcc gccgttgccg tagccggtgg acgggtcgat gcgcgtgccg taggccgtgg
     4681 ccgccgcctc gatctcggag gcggggatgc cggtgatctc ggccgccacc tcgggcttgt
     4741 actcggcggc gcgggccttg tagtgctccc acaccggctt gaccttcacg gtcttgccgt
     4801 ccttcagcgt gatctcgaac tcgccttcca gcgcggggtc gatggcgggg tcgaacggca
     4861 tgggatcggg cagccagccc tgcgtctggc ccggcagcag gttgtcctgc gcggcctcgc
     4921 ggccttggcg cgccttctcg gagtcgtagt tctcgccctc ccagaagccg ccggtctcgt
     4981 cgatgacgcc ttcctggtcg gcgttgaacc aggtgaacgc gccgtactcg tgctcgacgc
     5041 cctcggcctt cagcttctcc cagttgttgt cgtacacgag gaacttatag gggctgccgc
     5101 cctccttgat gtcgctttcc ttgagcagcg cggttttcac gtcccagtag gaaccgtcgg
     5161 tgcgcacggt ggggaagccg gagggctcca tgtcctcgca cacgaggaac ggggcgttcg
     5221 tccacttctt cacgtacagc tcgtcgtaga gcttcttctc gatgatgacg ttcgtccagg
     5281 ccagggccag ggcgccgtcg gtgccggggc gcaggtgctg ccagtagtcg gcttccttgc
     5341 ccatgttggc catgcggggg tcgacgctga tgtgcacgtc ggcgcgcgat gccacgtcca
     5401 ccgtggtgcg gcaggagtcg tcgtagttgg acagctcgga cgcgccgccc cactgcacgt
     5461 acactttcgg gcgcgtgatg gtttccatcc acgacatggc gaactgcgac accatggtgg
     5521 tggcgaagtg gcgcgggccc ttgcagatct gccaggcctc aacgttgttc ggcgtttcca
     5581 gcatgttctt caggatggac tccgagtgca tgcaccagat gcgcgacgtg cccacctggc
     5641 aggcgatggc ctcgccgccg tgcttcgcct tgatgtccat gaagttgtcc acgatggact
     5701 gcatggcctc gtcccacgag atgcgctgcc agccgggctc ctcgcccttc gggttcgtgc
     5761 gcttcatggg atagtgcaga cggtcggggt ggtacgccgc ctggatggac gactgcgact
     5821 tcgtgcagca gttgcccatg gactggaacg ctccctcgtc gccctcgacg cgtatggccc
     5881 gcccgttctg gacgatgacc ttgacgccgc actccatctt gccgcagccg cggcagcacg
     5941 ttttcacggc cacggtgtcg ttgccggaca cgctgctgta cgtctcttcg gccagcgcgg
     6001 tgtgcgtcga agcgccgaac gccgcggctg ccgcaccggt gatggcggcg gtcttgacga
     6061 acgtgcgtcg tgacatggtc aggttaccca tactcctcct ccttctttct cctcgttctc
     6121 gtcttgctcg tcttcgttcg tgcgatcggg gaggggggct gccgggtgaa gcctcgggct
     6181 ccgaaccggt gcaaagtccc cgtgtcgctt gggccgacca tacggatgcg agggggtgca
     6241 gagcatcgtc caaaggttgg aaagcaggtt cgaccgcatc ataaattctt atgatcttgt
     6301 gatcatgaaa aattcgatag tgctttgaac tggggaaact ttaaccgtag gtcataaatt
     6361 attctgaaac tagaatagtt tcctcaaaga gggccgctca tcttttccgt ataatcgaac
     6421 ccgtcgacag aggggtcggc gcgagcgacg ttatgtacgg gctcacaggg ggtggggcat
     6481 ggaaaagctt cggagtgcat gggataccgc gttggggacg agcctttccc tcaagcgcat
     6541 cgcgggcgcg gccttgcaat ggctttggat gctcatgatg ttctacagca tcgtgccgta
     6601 cggcttctcg tcggacgtgc gcggctcgct gtacgtcagc ctgatcatat cgctggtggc
     6661 catggtggtg accatgctgt tcgtctccgt catcgtgcgg cgcgagcggg tgagcggcaa
     6721 ccgctccatc gtgctgggat cggccatcat gatgagcgcg ggcagcgtgc tcacgccgtt
     6781 ctccgatcct accacgcccg tcggcatgct ggtgctgggc ctgagcgcgg tcatgaccgg
     6841 cacgggctcg gccgtgctgt tcttgtgctg gatcgagctc gagtcggggc tgggaggccg
     6901 cttggcgctt gtggagcttg cctcgtcgct gtgcatcgcg ttcgtcgtgg ggttcttgct
     6961 gatcgtaggg ccggcgatac ccgtcatcgt gctcatcgtt gtcatgcccg tgctttcggc
     7021 ggcagctctc aggcgcagcg cgcccgatac gccgcagctg ccgcgcgagc ccgagcagcc
     7081 tttgtcgcgc catacggcga ccctgttcgc gaaggcgctt ctgggcgcgg tgctcatcgg
     7141 catgttgatg gggttcttcg acgtggtgtc ggggttcaaa acctacgagg tgcaggacat
     7201 cttcggcacg tacctgtttc tggcgggctt catcggagcg ttggccatct gcctgatagc
     7261 cgtgttcctg cagcgcgaca gcgtgttctt ctcgtaccgc gtctccatgc tgatgctgtg
     7321 cttgggatgc ttgtccacgc cgttcatggc cgacaacaac acgtattcga gcgcgcttat
     7381 cttcggcggc taccactgct tcgtgctggt gctgtgcgtg gtgtgcatcg acgtggcgtc
     7441 cagcttccgc gtgagcccgg cgcggacgat cggcctgggg ttcgtggcgc tgtacggcgg
     7501 cgagaccgca ggccagctgt tcgcccattc gctggaggcg accggggtca gcatgttcga
     7561 cctcacgctg gtcacgctgg tggccgtgtc gctgctgttc atcgcgcatt tgttcctgtt
     7621 cacggagacc gacctcatca aaatcggcat cggcgaggtc agcctgatgg gcacggtgcc
     7681 cgaagaggcc gccgacgacc cggccgaccc ggtggatccg tgcgcgctga tcgcggagcg
     7741 cttcgccctg tcgccgcgcg agaccgacgt gcttccgctg ctgctggagg gccgcaccat
     7801 ctcgcgcatc caggagacgc tgttcatctc ggcgggcacg gtgagcacgc atatccgcca
     7861 catctaccag aagacgggcg tggacaatcg ccaggagctc atcgacctgg cccacgagac
     7921 ggccgcgcag gacgaggcgg tctgaggcga tggagacggc gtatctgcac gagttcgcga
     7981 aggtggccga ttgcggcagc ttcacggccg ccgcgcgcga gctgcatctg acgcagtcga
     8041 cgctgagcaa gcacgtggcc ttgttggagc gcgaattcgg ggccgacctg ttcgtgcgcg
     8101 accgcagcgg cgtgagcctg accgaggcgg gcggcgtgct gtacgcccag gcgcggcaga
     8161 tggagaagct gctgcgcgcc accgagctgc tggtgcgcgc ggcgcgcgac gggcagggtt
     8221 cggcgggcgc gggggttgcg ggcgcgacgg cgggcgcagc ggcgggcggg ggtgccgtcg
     8281 acggctcgcc tgcggccgcg tgcgacacgg cgcttcgctg caagtgccgg ctcgcggccg
     8341 agcgctgcgg gctcgaccgg cgcgagctgg gcgcgctcat cctgtacgtg gaggagcgcg
     8401 gcttcgaggc gatccagggc gagctggcgc tgacgcgcga cgaggtggcc gacgtgctgg
     8461 gcagcgtgta ccgcaagctg ggcgtgggcg acaagcagga ggcgctcgat ttcatccatt
     8521 ccgtttcgga atgaactagt cgactcacac tggctcacct tcgggtgggc ctttctgcgt
     8581 ttatatacta gagagagaat ataaaaagcc agattattaa tccggctttt ttattattta
     8641 attcctgtta ccgctggttg ccacgacgcc ctcgaagtct ccttcgaggg cgttctgtcg
     8701 tttgcaagaa gggtctggag aggcaaatcg cgctcgaagg ggcttcgagg ccggggtagg
     8761 tcgggagggt ggaatccgga tgtttcacgt gaaacatccg ttcttttacg gccggtgttt
     8821 cgcgtgaaac attttgcgga aaggactcct tcccttcccg tgcgccaccc caacgccccg
     8881 ctcctagcgc tcgtacacct ggtcgcagtt gagcagatcc ttcatggagt gccagatctg
     8941 gtgcgggttg cccggcgggt tcttgagcgg cttcatcgga tagcgctcct gcgcgcccga
     9001 cagcagatcg acggcgaaca cttcgtcgga cagctcgagc gcgccttccg ggcagatgtc
     9061 ggtgcagcag cggcacttca cgcaatcgac cggacgatgc gtcacgccga tcgagccgtc
     9121 ctcacccgcg tacttcgcga tggccccggt ggggcagaac gtggcgcaca tctggcacga
     9181 cgagcacttc tcgggatcga tgatcacatg gccccacaag cgcgtgtcca tgagcacatc
     9241 ctgcggctgc cccaacgaat cgagcgcggc cagcaaacgt tcgcggcggt cgggaatgaa
     9301 gtgaggcagc gtgccgtcct tgcccacctt cacgaacctc ggctcccgcc gctcctccac
     9361 acccaatgcg tccttgacgg cgtagtcagc cgtcaccgcc gcaacgttct tagcctcgtc
     9421 cttcatgctg gagaagaagc tccgacggct cgcgtcgtag ccctcgtcgc ccgcccggcg
     9481 cacggatgag ggaagctttt ccgccaggtc gatgcgcata tcgttgttcc aggttgcaag
     9541 cagcgcgctt gccgtgtcgc acacaacccg cgccatctcg aaacccgaga catgctcgca
     9601 ttgcgcgcag cgcgcctgca cgaggctcac gcggcgcacg cccaacgccg ccagcgtgac
     9661 cagcaggctc tcctccacgc gccccaggca cgtgacgccc accaccttct cggggtcgta
     9721 caacccgtcg gccgcggcaa gcagctgatc gcaggcgata accacctgtc cgtcggccac
     9781 gtcagccgcc gcgcggcatg cttgcagaag ctcggcatcg ttcggacgat gcgcttcaag
     9841 cgcgcaggtg ggacacacgg tggcgcaggt tccgcacccg atgcaccgct cgggttcgat
     9901 gaccagctcg ttgtcgtcat aggagatgca accgctcgtg cacacgtcgg cgcacttcat
     9961 gcacgttgcg ttgcgattgc gcacaatagc gcaacgaccc tgatgaaccg taatgtcggc
    10021 gctctgaagt tgttcgagca gaccgaggaa attcgtcacg tgagccatga cttcaccctt
    10081 tctcgtctac ccccttgcac cgcacagtat aaccactttc gccgtctcac gcgagaaaaa
    10141 acgcgcttga caccttcgac aggcctccct atcatgtggc cttggggagt ccgccggcac
    10201 gctgatcgcg tgcgaagcgg acattgtttg caaaagtggg aacctcaggg gtgaggtttc
    10261 gcggtgctgt gcgccgtaag tgcaaacaat gtctgacgag gggcggaaca tgactcacga
    10321 ttccacgaac cagcatcttt ctcttgagcc cgactacgac gggttcgatc cacttgaaga
    10381 tttcgaagac gatgcgaccg aggtcgaagg ccgtgccgac gagttcgtcg ccacggtgag
    10441 cgccgccgaa gacgcgctcg ctcacgacgc gatggcaccc gccacgcccg ccatctgcga
    10501 aaagcccgct gaagagcgca tcgccgactt gttggaggcc atggcgccgc gccgcacggt
    10561 gctgctgggc atcctggccg cctgcgccga gccgcagccg gcatccgccg tgaacgctcg
    10621 cgtcgacgag ctgcaggaga acaacttctc ggtgtacagc gcggccaacc tgtgctcctt
    10681 gctggagaag gcgggcgcca tcgagcgcgt cacggccgag ggcgaacccg cggaggacat
    10741 cgaaatcgag ccacagaccg tcgtcgtcga cggcgtcgaa tacctggaag cccgcgaacc
    10801 cgtcgagatc tactggcaca tcaccgagcc gggccgcgcg gcgcttgagg ccgacaaacc
    10861 gctcgagcgc ctgcgcgccc tcctggacga agatgccgcc tacgcgccca tctaccagcg
    10921 catcctccgc ttgtgcacag cagacggcgg cgccacgaca cccgccatca acaacgcggt
    10981 cgaccacaac ccgctggtgc agaagccgcg cttctacgcg ccccacttcg tcgaccgcct
    11041 ggagaaatgc gacgcgctgg cctggaagaa ggcgtggtgc atcaccgaca tcggacgcgc
    11101 cggcctcgat atgctggcag acgtcgtaga cgagaacgcg cccgccaccc agcccgaaac
    11161 ccccgcaacc cctgatcccg ccgcatccaa ggaggactaa ccatgaccga agcaaccgcg
    11221 cccgcaaccg agaccctcgc cgacgaagcg accatgcagg agctcgtcaa actgaacgag
    11281 cagcgcgccg ccacgtacgg cctgctgtcg cgcctgtacc gcgtggaggt ggaccagccc
    11341 cttctcgacg agctgcgcag catgcgcttc cccgccaaga cgggcaacga gaacgtggac
    11401 gagggctacc gcctgctggc cacgtacctg agcaacacct gggacaacag cgtcaccgac
    11461 ctcgccatcg actacgtgcg cgtgttcatc ggacacggca tcgacgcgtt ctcggccgcc
    11521 tacccgttcg aatcggtgta cacctccgag aagcgcctgc tcatgcagga agcacgcgac
    11581 gaggtgctgg ccatctaccg cagcgcaggc ctcgacaagc aggacacatg gaaagagggc
    11641 gaggatcata tcgcgctcga gctggaattc gagcagatca tggccacgcg cacggtcgaa
    11701 gcgctgcgca aaggcgacga agacgaggcc gtcgcgctgc tgaccacgca gaagaacttt
    11761 ctcgaagacc atctggcggc atgggctccc atgatgacct ccgacatgaa gcgattcgcg
    11821 caaaccgact tctaccgggg cttggcgtac ttgacggacg ggttcctcca aaccgaccaa
    11881 acgttcctcg acgacgtgct ggccgaagac taactgcaca ggatcgaaag aaggagcgat
    11941 ggacaacaag caggagaaaa cctacgccgg catgacgcga cgcacgctgt gcctgggcgc
    12001 aggcggcgct gcggtcatgc taggtctagg cggattgaag tacgcgggat cgcaaccgct
    12061 cgtgcgcccg ccgggaggcc aggacgagga acggctcatc tcggcctgca tccgctgcga
    12121 gaagtgctac gagatctgcc cgcgcgacgt catcgcgccc gcccacctcg aggacggtgt
    12181 gctgaacatg cgcaccccca cgttcgactt ctcggccaac tggtgcgact ggtgcgccga
    12241 agagcacgac ggcaccccgt tgtgcgtgag cgcctgcccc accgaagcgc tcaagctgcc
    12301 cgacgacgcc acggccgaga acaccatcat cggcaaagcc gttatcaaca cggactggtg
    12361 cttggcgtac aagctgatcg gatgccggtt ttgctacgac gcctgtccct acgaggccat
    12421 ggagctcgac gagaacaacc gccccgtcgt gatcgaggac aagtgcaacg gctgcggcgc
    12481 gtgcgagagc gtgtgcgtct cgctgcagaa cggctccatc tccgaaggag ccaccgcgcg
    12541 cgccatcgtc gtccacccgc ttgacgaagt tgaaggataa ggtgccctca tgaagaagaa
    12601 ctccaagaag ctgcgcaccc tcgtcgcact ggccatcgtg gccgtcgtcg ccgtcggctt
    12661 cgtcacgaac gtcgggatcg gcaccatgtc ggcaccgggc atctgggaca tctccatcct
    12721 atgcccgctg ggcgccttgg gaacgatgct cgcctccaag atgatggtgc cgcgcgccgt
    12781 aatctcgctg atcgtgatgg tgctgctcat cgttctgttc gcccgcgcgt tctgcggatg
    12841 gatttgcccc gtgccgctcg tgcagaagtt gcgcggcttg ttcgcgaaga agcaacccgt
    12901 cgaacgcggc gacgatgccg atcctgccgc ggcgccgctc acgaaagtcg agatcgacgc
    12961 gctgggcgtt tcatgctcga aggggtcgaa gaatggcagc ggatgcgcgt cctgcgcctc
    13021 caagcgcgga gccgcgctcg atgctcgcca cttcgtgctg ggcggcgcgc tggcgtccac
    13081 cttcatcttt ggattcccgg tgttctgcct cgtctgcccc atcggactca cgttcgccac
    13141 catcctgctg gtcatcaacc tgttctcaca cggcgatgtc acctggtcgc tcatcgtggt
    13201 gcccgcgctg cttatcgccg aggtgctgct gttcaagaag tggtgccata agctgtgccc
    13261 gctttcggcg ttcatgagcc tgatcgcgaa ggcgaacaag accttcgtgc ccacgatcga
    13321 cgatgcgaag tgcctggaaa cggcgaaggg cggcgcgtgc ggggcctgcg gccgcgtctg
    13381 cgaggagggc atcgacccgc gccacccgca gctttcgcag gcggcatgga gcgagtgcac
    13441 caagtgccgc gcctgcgtgg acgcatgccc tgcgaacgcg atcaccatgc cgctgctgcc
    13501 gcggcgaacg ccgtctaccg cagaacctgt cgccgtgcca acgaacgccg agaagatcga
    13561 cgcggagaat gcgcgccccg ttccgtaaag cccctcctgc gcgacccggc tcgcagctgc
    13621 agcggtctgg tcgcgcatcc ggaatcgggc cgagcccgac cccgggcaaa aggcggcttg
    13681 gcaagggctt tgggggacga gccgcctttt gcccgggagc gacatccccg ctccccctcc
    13741 ctgcgaaaag agggatgccg cttccggcct tcatctgaca accacgacgt gggaggacga
    13801 aaacccatgg catacgaaac gacaagcgaa tcgaccgagg ccctgctcgt caccggaggc
    13861 gtcggcaccg gcaaaacgca acgtctcgtc gagcgcgctg ccgcgctgct ggccgaaaac
    13921 gccgccgcgg gcaacgtgct cgtgctgtgc gcgacgcccc aggccgcacg cctgttctcg
    13981 gagcggttga cagcccgcgt cggtgccaag cgcgctgccg gcgcaaccgt cgccaccgca
    14041 cgcgccttag cgctcgacgt gctggccgac gacggcgcgc gccgctggtc ggggcgcgag
    14101 ccgcgactac tgaccgcctt cgaagagacg ttcctcatgg aggacatgaa ggtgagcggg
    14161 ctgcggccga agcggctgcg cgagatgctc aagttcttct accgctcgtg gaccgagctg
    14221 gcggacgacg atcccgattg gctgctaccg ggcgaggagg ccgatgtcca tacgctgttg
    14281 aaagagaacc tggtcttcat gcgcgccgtc atagaacccg aagcggcgaa ccttgctgca
    14341 cgctacctcc gagaacacga cgaagcgcgg gcggcgcatt cctacgcgca cgtgctgatg
    14401 gacgactacg cctgcgcaag ccgtgcctcg cagacgctgg cgaatctcgt cgcaacctgc
    14461 tcgatcaccg tggcgggcga cgcggaaacc tgcatcgaga catacgattc gtacccctat
    14521 gccgccggaa tcgacgaatt cctcgcaacg catcccgatg ctgcttgcga gcacctggat
    14581 gccgacgcgc ccgatgccgt cgaacgggtc gctctggcaa gcgacacgcc cgaagccgag
    14641 ttcgaacagg tggcgcgcac ggtggccgaa gccgtcgccg caggaacgcc cgcacggcag
    14701 atcgtcgtgg ccgtaccgaa cagcgtgtgg tcgcgcaaca tcgcaaccgc tttgcacgca
    14761 cgaggcgtgc caaccgaagc gctcgccatc tcgcagcccc tgcgcggcga cgtgcgggac
    14821 aacgcccgct gcacgcctgc gcgcatcgcc acggccctca acctcgtggc gaacccagac
    14881 gacaccgcgg cttggcggtg ctggtgtgga ttcggcgact acctggtgaa cagcgcggct
    14941 ttcgccagcc tgcggacttg cgcgaaggag cgcagtatcg gcctcgtgga cgctttggag
    15001 atgctaagcg aaaacgactg cgagcacgta gtgggcgcgc aacgcgtggc gaccgcctac
    15061 aaggacggtc gcgcgctcat cgagcaggta cgaggattgc ggggcacggc actgctggac
    15121 gagctgacgc gcatcgtcac cgacggagcg gaatcccaag cgcccgccgt tctgcgcgag
    15181 ctctgcctcg agcacggcaa cgcagccgat gaaaacccgg aaagccttta cgatgccgat
    15241 gctgcggcga tggcgcggcg catggccgag cgcctgctcg cgcccacgct ggaggttgcc
    15301 gacgcggtag ccgtcgtccc ctacgacttc gcctgcggct tctcgcccga cctgctgatc
    15361 gtcgccggct tcgtgaacgg tttcatcccc tcccgcgact atttcgacgc cacggtgacc
    15421 ccgcccgagc gccaggagaa gatgcgcgcc gtcgacgaac gacgcgtgcg cgcgctcaca
    15481 ggaaaggccg gccgcgcggt ggcgctgtcg tacttcacgt ccaccgacct cgaaagcgcc
    15541 ggcaagctga agctcaagat cagtcgcatc cgcctcaaga acggtatccg cacctgcact
    15601 atagagccca gcctattcct ggagcaaatc gcagcgcagg agtagtaaaa aaaggatcaa
    15661 ttttgaactc tctcccaaag ttgatccctt aacgatccct gcaggagaag gccatcctga
    15721 cggatggcct ttttgaagct ttctagaatc gggggccccc ggggggattc tagaacaatg
    15781 gaagtcctcg tctcgccctc gaattagccc gcttaatgag cgggcttttt ttgaattaat
    15841 tctcgcgagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
    15901 taatgtaagt tagcgcgaat tgcaagctgg ccgacgcgct gggctacgtc ttgctggcgt
    15961 tcgggagcag aagagcatac atctggaagc aaagccagga aagcggccta tggagctgtg
    16021 cggcagcgct cagtaggcaa tttttcaaaa tattgttaag ccttttctga gcatggtatt
    16081 tttcatggta ttaccaatta gcaggaaaat aagccattga atataaaaga taaaaatgtc
    16141 ttgtttacaa tagagtgggg ggggtcagcc tgccgccttg ggccgggtga tgtcgtactt
    16201 gcccgccgcg aactcggtta ccgtccagcc cagcgcgacc agctccggca acgcctcgcg
    16261 cacccgctgg cggcgcttgc gcatggtcga accactggcc tctgacggcc agacatagcc
    16321 gcacaaggta tctatggaag ccttgccggt tttgccgggg tcgatccagc cacacagccg
    16381 ctggtgcagc aggcgggcgg tttcgctgtc cagcgcccgc acctcgtcca tgctgatgcg
    16441 cacatgctgg ccgccaccca tgacggcctg cgcgatcaag gggttcaggg ccacgtacag
    16501 gcgcccgtcc gcctcgtcgc tggcgtactc cgacagcagc cgaaacccct gccgcttgcg
    16561 gccattctgg gcgatgatgg ataccttcca aaggcgctcg atgcagtcct gtatgtgctt
    16621 gagcgcccca ccactatcga cctctgcccc gatttccttt gccagcgccc gatagctacc
    16681 tttgaccacc atggcatcag cggtgacggc ctcccacttg ggttccagga acagccggag
    16741 ctgccgtccg ccttcggtct tgggttccgg gccaagcact aggccattag gcccagccat
    16801 ggccaccagc ccttgcagga tgcgcagatc atcagcgccc agcggctccg ggccgctgaa
    16861 ctcgatccgc ttgccgtcgc cgtagtcata cgtcacgtcc agcttgctgc gcttgcgctc
    16921 gccccgcttg agggcacgga acaggccggg ggccagacag tgcgccgggt cgtgtcggac
    16981 gtggctgagg ctgtgcttgt tcttaggctt caccacgggg cacccccttg ctcttgcgct
    17041 gcctctccag cacggcgggc ttgagcaccc cgccgtcatg ccgcctgaac caccgatcag
    17101 cgaacggtgc gccatagttg gccttgctca caccgaagcg gacgaagaac cggcgctggt
    17161 cgtcgtccac accccattcc tcggcctcgg cgctggtcat gctcgacagg taggactgcc
    17221 agcggatgtt atcgaccagt accgagctgc cccggctggc ctgctgctgg tcgcctgcgc
    17281 ccatcatggc cgcgcccttg ctggcatggt gcaggaacac gatagagcac ccggtatcgg
    17341 cggcgatggc ctccatgcga ccgatgacct gggccatggg gccgctggcg ttttcttcct
    17401 cgatgtggaa ccggcgcagc gtgtccagca ccatcaggcg gcggccctcg gcggcgcgct
    17461 tgaggccgtc gaaccactcc ggggccatga tgttgggcag gctgccgatc agcggctgga
    17521 tcagcaggcc gtcagccacg gcttgccgtt cctcggcgct gaggtgcgcc ccaagggcgt
    17581 gcaggcggtg atgaatggcg gtgggcgggt cttcggcggg caggtagatc accgggccgg
    17641 tgggcagttc gcccacctcc agcagatccg gcccgcctgc aatctgtgcg gccagttgca
    17701 gggccagcat ggatttaccg gcaccaccgg gcgacaccag cgccccgacc gtaccggcca
    17761 ccatgttggg caaaacgtag tccagcggtg gcggcgctgc tgcgaacgcc tccagaatat
    17821 tgataggctt atgggtagcc attgattgcc tcctttgcag gcagttggtg gttaggcgct
    17881 ggcggggtca ctacccccgc cctgcgccgc tctgagttct tccaggcact cgcgcagcgc
    17941 ctcgtattcg tcgtcggtca gccagaactt gcgctgacgc atccctttgg ccttcatgcg
    18001 ctcggcatat cgcgcttggc gtacagcgtc agggctggcc agcaggtcgc cggtctgctt
    18061 gtccttttgg tctttcatat cagtcaccga gaaacttgcc ggggccgaaa ggcttgtctt
    18121 cgcggaacaa ggacaaggtg cagccgtcaa ggttaaggct ggccatatca gcgactgaaa
    18181 agcggccagc ctcggccttg tttgacgtat aaccaaagcc accgggcaac caatagccct
    18241 tgtcactttt gatcaggtag accgaccctg aagcgctttt ttcgtattcc ataaaacccc
    18301 cttctgtgcg tgagtactca tagtataaca ggcgtgagta ccaacgcaag cactacatgc
    18361 tgaaatctgg cccgcccctg tccatgcctc gctggcgggg tgccggtgcc cgtgccagct
    18421 cggcccgcgc aagctggacg ctgggcagac ccatgacctt gctgacggtg cgctcgatgt
    18481 aatccgcttc gtggccgggc ttgcgctctg ccagcgctgg gctggcctcg gccatggcct
    18541 tgccgatttc ctcggcactg cggccccggc tggccagctt ctgcgcggcg ataaagtcgc
    18601 acttgctgag gtcatcaccg aagcgcttga ccagcccggc catctcgctg cggtactcgt
    18661 ccagcgccgt gcgccggtgg cggctaagct gccgctcgga cagttcgagg ctggccagcc
    18721 tgcgggcctt ctcctgctgc cgctgggcct gctcgatctg ctggccagcc tgctgcacca
    18781 gcgccgggcc agcggtggcg gtcttgccct tggattcacg cagcagcacc cacggctgat
    18841 aaccggcgcg ggtggtgtgc ttgtccttgc ggttggtgaa gcccgccaag cggccatagt
    18901 ggcggctgtc ggcgctggcc gggtcggcgt cgtactcgct ggccagcgtc cgggcaatct
    18961 gcccccgaag ttcaccgcct gcggcgtcgg ccaccttgac ccatgcctga tagttcttcg
    19021 ggctggtttc cactaccagg gcaggctccc ggccctcggc tttcatgtca tccaggtcaa
    19081 actcgctgag gtcgtccacc agcaccagac catgccgctc ctgctcggcg ggcctgatat
    19141 acacgtcatt gccctgggca ttcatccgct tgagccatgg cgtgttctgg agcacttcgg
    19201 cggctgacca ttcccggttc atcatctggc cggtggtggc gtccctgacg ccgatatcga
    19261 agcgctcaca gcccatggcc ttgagctgtc ggcctatggc ctgcaaagtc ctgtcgttct
    19321 tcatcgggcc accaagcgca gccagatcga gccgtcctcg gttgtcagtg gcgtcaggtc
    19381 gagcaagagc aacgatgcga tcagcagcac caccgtaggc atcatggaag ccagcatcac
    19441 ggttagccat agcttccagt gccacccccg cgacgcgctc cgggcgctct gcgcggcgct
    19501 gctcacctcg gcggctacct cccgcaactc tttggccagc tccacccatg ccgcccctgt
    19561 ctggcgctgg gctttcagcc actccgccgc ctgcgcctcg ctggcctgct gggtctggct
    19621 catgacctgc cgggcttcgt cggccagtgt cgccatgctc tgggccagcg gttcgatctg
    19681 ctccgctaac tcgttgatgc ctctggattt cttcactctg tcgattgcgt tcatggtcta
    19741 ttgcctcccg gtattcctgt aagtcgatga tctgggcgtt ggcggtgtcg atgttcaggg
    19801 ccacgtctgc ccggtcggtg cggatgcccc ggccttccat ctccaccacg ttcggcccca
    19861 ggtgaacacc gggcaggcgc tcgatgccct gcgcctcaag tgttctgtgg tcaatgcggg
    19921 cgtcgtggcc tgcccgctct aatgcccggt tggcatggtc ggcccatgcc tcgcgggtct
    19981 gctcaagcca tgccttgggc ttgagcgctt cggtcttctg tgccccgccc ttctccgggg
    20041 tcttgccgtt gtaccgcttg aaccactgag cggcgggccg ctcgatgccg tcattgatcc
    20101 gctcggagat catcaggtgg cagtgcgggt tctcgccgcc accggcatgg atggccagcg
    20161 tatacggcag gcgctcggca ccggtcaggt gctgggcgaa ctcggacgcc agcgccttct
    20221 gctggtcgag ggtcagctcg accggcaggg caaattcgac ctccttgaac agccgcccat
    20281 tggcgcgttc atacaggtcg gcagcatccc agtagtcggc gggccgctcg acgaactccg
    20341 gcatgtgccc ggattcggcg tgcaagactt catccatgtc gcgggcatac ttgccttcgc
    20401 gctggatgaa gtcggccttg gccctggccg attggccgcc cgacctgctg ccggttttcg
    20461 ccgtaaggtg ataaatcgcc atgctgcctc gctgttgctt ttgcttttcg gctccatgca
    20521 atggccctcg gagagcgcac cgcccgaagg gtggccgtta ggccagtttc tcgaagagaa
    20581 accggtaagt gcgccctccc ctacaaagta gggtcgggat tgccgccgct gtgcctccat
    20641 gatagcctac gagacagcac attaacaatg gggtgtcaag atggttaagg ggagcaacaa
    20701 ggcggcggat cggctggcca agctcgaaga acaacgagcg cgaatcaatg ccgaaattca
    20761 gcgggtgcgg gcaagggaac agcagcaaga gcgcaagaac gaaacaaggc gcaaggtgct
    20821 ggtgggggcc atgattttgg ccaaggtgaa cagcagcgag tggccggagg atcggctcat
    20881 ggcggcaatg gatgcgtacc ttgaacgcga ccacgaccgc gccttgttcg gtctgccgcc
    20941 acgccagaag gatgagccgg gctgaatgat cgaccgagac aggccctgcg gggctgcaca
    21001 cgcgccccca cccttcgggt agggggaaag gccgctaaag cggctaaaag cgctccagcg
    21061 tatttctgcg gggtttggtg tggggtttag cgggctttgc ccgcctttcc ccctgccgcg
    21121 cagcggtggg gcggtgtgta gcctagcgca gcgaatagac cagctatccg gcctctggcc
    21181 gggcatattg ggcaagggca gcagcgcccc acaagggcgc tgataaccgc gcctagtgga
    21241 ttattcttag ataatcatgg atggattttt ccaacacccc gccagccccc gcccctgctg
    21301 ggtttgcagg tttgggggcg tgacagttat tgcaggggtt cgtgacagtt attgcagggg
    21361 ggcgtgacag ttattgcagg ggttcgtgac agttagtacg ggagtgacgg gcactggctg
    21421 gcaatgtcta gcaacggcag gcatttcggc tgagggtaaa agaactttcc gctaagcgat
    21481 agactgtatg taaacacagt attgcaagga cgcggaacat gcctcatgtg gcggccctcg
    21541 cgagaattaa ttcagataaa aaaaatcctt agctttcgct aaggatgatt tctagcgatg
    21601 accctgctga ttggttcgct gaccgtagac tggttggctt ggtttcatca gccatccgct
    21661 tgccctcatc tgttacgccg gcggtagccg gccagcctcg cagagcagga ttcccgttga
    21721 gcaccgccag gtgcgaataa gggacagtga agaaggaaca cccgctcgcg ggtgggccta
    21781 cttcacctat cctgcccggc tgacgccgtt ggatacacca aggaaagtct acacgaaccc
    21841 tttggcaaaa tcctgtatat cgtgcgacat cgcgcgcgcg cgcgcggatg ctagcggcgc
    21901 gccaaaaaaa agcccgctca tta
//
