LOCUS       sequence-463816-       11674 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses His-tagged Gordonibacter pamelaeae DSM 19378 (+)-Eldh2 
            with a cumate inducible promoter in Gordonibacter urolithinfaciens 
            DSM 27213 for protein purification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11674)
  AUTHORS   Dong X, Bae M, Le C, Aguilar Ramos MA, Balskus EP
  TITLE     Enantiocomplementary Gut Bacterial Enzymes Metabolize Dietary 
            Polyphenols.
  JOURNAL   J Am Chem Soc. 2025 Mar 5;147(9):7231-7244. doi: 
            10.1021/jacs.4c09892. Epub 2025 Feb 24.
  PUBMED    39993729
REFERENCE   2  (bases 1 to 11674)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.4c09892"; journalName: "J Am Chem Soc"; date: 
            "2025-03-5- 5"; volume: "147"; issue: "9"; pages: "7231-7244"
COMMENT     Sequence Label: pXD80ENSF27-Pct3-(+)-Eldh2(nHis6)(GpDSM19378)
FEATURES             Location/Qualifiers
     source          1..11674
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(19..813)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     primer_bind     1300..1318
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    1475..1494
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     CDS             complement(1497..2108)
                     /codon_start=1
                     /gene="cymR from Pseudomonas putida"
                     /product="cumate repressor (Mullick et al., 2006)"
                     /label=CymR
                     /translation="MSPKRRTQAERAMETQGKLIAAALGVLREKGYAGFRIADVPGAAG
                     VSRGAQSHHFPTKLELLLATFEWLYEQITERSRARLAKLKPEDDVIQQMLDDAAEFFLD
                     DDFSISLDLIVAADRDPALREGIQRTVERNRFVVEDMWLGVLVSRGLSRDDAEDILWLI
                     FNSVRGLAVRSLWQKDKERFERVRNSTLEIARERYAKFKR"
     protein_bind    2512..2539
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P2 operator sequence from the p-cmt 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     CDS             2600..2617
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      5298..5325
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      5457..5484
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(5916..6767)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(6754..7593)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(8104..9075)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELSERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     oriT            10314..10401
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     rep_origin      10743..11137
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     terminator      complement(11300..11343)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     oriT            11439..11548
                     /note="incP origin of transfer"
ORIGIN
        1 ggcgggctgg ccggccttct aaaacaattc atccagtaaa atataatatt ttattttctc
       61 ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg acatactgtt cttccccgat
      121 atcctccctg atcgaccgga cgcagaaggc aatgtcatac cacttgtccg ccctgccgct
      181 tctcccaaga tcaataaagc cacttacttt gccatctttc acaaagatgt tgctgtctcc
      241 caggtcgccg tgggaaaaga caagttcctc ttcgggcttt tccgtcttta aaaaatcata
      301 cagctcgcgc ggatctttaa atggagtgtc ttcttcccag ttttcgcaat ccacatcggc
      361 cagatcgtta ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc tattcgtata
      421 gggacaatcc gatatgtcga tggagtgaaa gagcctgatg cactccgcat acagctcgat
      481 aatcttttca gggctttgtt catcttcata ctcttccgag caaaggacgc catcggcctc
      541 actcatgagc agattgctcc agccatcatg ccgttcaaag tgcaggacct ttggaacagg
      601 cagctttcct tccagccata gcatcatgtc cttttcccgt tccacatcat aggtggtccc
      661 tttataccgg ctgtccgtca tttttaaata taggttttca ttttctccca ccagcttata
      721 taccttagca ggagacattc cttccgtatc ttttacgcag cggtattttt cgatcagttt
      781 tttcaattcc ggtgatattc tcattttagc catgatgttt aactttgttt tagggcgact
      841 gccctgctgc gtaacatcgt tgctgctcca taacatcaaa catcgaccca cggcgtaacg
      901 cgcttgctgc ttggatgccc gaggcataga ctgtacccca aaaaaacagt cataacaagc
      961 catgaaaacc gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt
     1021 gcgtgagcgc atacgctact tgcattacag cttacgaacc gaacaggctt atgtccactg
     1081 ggttcgtgcc ttcatccgtt tccacggtgt gcgtcacccg gcaaccttgg gcagcagcga
     1141 agtcgaggca tttctgtcct ggctggcgaa cgagcgcaag gtttcggtct ccacgcattc
     1201 gaaaccggta tcccgccgcc gccgccggat accggtttcg aattgagatt gccacctgac
     1261 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     1321 tttcgtcttc aaggcgaaaa aaccccgccg aagcggggtt ttttgcgaaa tataatgacc
     1381 ctcttgataa cccaagaggg cattttttag gaacggttat ttgattatta atccggcggc
     1441 aactgaaacg attcggatcc tgtattacta ttctgaattc gcggccgctt ctagatctag
     1501 cgcttgaatt tcgcgtaccg ctctcgcgca atttcgagtg tcgagttcct gacacgctca
     1561 aagcgttctt tgtccttctg ccataggcta cgaacagcaa gcccacgcac cgaattgaat
     1621 atcaaccaaa ggatatcttc tgcatcatca cgcgaaagac cacggctcac cagaacacca
     1681 agccacatat cctcgacgac aaaccgattc ctctctaccg tgcgctgaat accctcgcgt
     1741 aacgctgggt cccggtcggc agccacaatc aaatcaaggc tgatagagaa gtcatcgtcg
     1801 aggaaaaatt cggcggcgtc gtccagcatt tgctggatga cgtcatcctc tggcttcaat
     1861 ttcgctaatc gagcccgact gcgttcggtg atctgttcgt aaagccattc aaaagtggca
     1921 agcagaagct caagctttgt cgggaaatga tggctctgcg ctcctctgga gacaccagca
     1981 gcaccgggca catctgcgat ccggaatccc gcgtaacctt tttcccgtaa aacccccagg
     2041 gccgctgcaa tcaacttgcc ctgggtctcc attgcgcgct ctgcctgtgt tcttctcttt
     2101 ggactcatga tcaccagcga cctgctttcg ttcgtccggg catcacccgg gttactatct
     2161 agtatcaata actagattat tggttgaaac gtactatgtc aagcagttct aaagattgac
     2221 tccaaacggt cgccttgcta tgctcgtttc gtttcctccg cacgcgcgcg aaaggctcct
     2281 tcacggagcc aatgtacgca aaaaaccccg cttcagcggg gttttttcgc caaaaaaaac
     2341 cccgccctgt caggggcggg gttttttttt ttagggccct actgactgtg agcccgctcg
     2401 atcaacggat cggtaaaata cgggcgaaaa agagaatccc gagctttttt gttgccgcat
     2461 agcgtatagt aagcatgtgc cctcgaggtg agggcgagtg ttgcggccag taacaaacag
     2521 acaatctggt ctgtttgtat tatgcagcac acagtgttac ctcctcgggg acgaggagag
     2581 gaaagaggat ttaaaaatgc atcaccatca ccatcacgac tacgacatcc cgactaccga
     2641 tcaggccaat agctactacg aggcccataa gggcgaggtg gagtggcgcg acggcgacta
     2701 cacggtgacg cgcacgacgg tgtggtcggg gcccggttgc cacgacggct gcggcgtgct
     2761 gtactacacg aaggacggca agctggagaa ggtggagggc gaccccgact gccccttcaa
     2821 ccagggcacg ctgtgcatgc gctgcctgga gatgcccgaa gccgtgaacc atcccgaccg
     2881 gccgaccggt ccgctcaagc gggcgggcga gcgcggcgag aacaagtggg agtccgtcac
     2941 ctgggacgag gcctacgaca ttatcgagga gaaggtgcgc gccatctgga aggactacgg
     3001 cgccgagtcc atcgcgtgca tgatcggcac cggtcgcaac aactgctggc agatcccgta
     3061 cctgtgctat gcggcgttcg gctcgccgaa cttctgcctg ggattcctct cgggcgagtc
     3121 gtgcttccag ccacgctcgg cggccatggc ctccatgaac ggcgacttcc tcatcgccga
     3181 catgtcacag cagttcgaga gccgctacga caccgagaac accgagtggg ttccgcctga
     3241 gatgatcgtg gtctgggggt gcaaccccat cgcctcgaac tccgacaact tctacggagg
     3301 ctgggtcgtc gactgcatgc agcgcggcag ccggttgatc accgtcgacc cgtcgctcac
     3361 ctggttggcg gcccgttccg actattggct gcgcctgcgc cccggcaccg acggcgcgct
     3421 cgccctggcc atgctcaacg tcatcatctc cgaggacctc tacgaccgcg agttcgtcga
     3481 gaagtggtgc tacggcttcg acgagcttgc cgagcgcgtc aaggaatgga cgccggagcg
     3541 ggcggaggcg gtgtgctggg ttccgaagga caccataatc ggggcggcgc gcgcctacgc
     3601 gaaggccaag cccgccacca tccactgggg cctgcccatt gaccaggcgg ttgtcggcat
     3661 ccccacggcg caggccatca acgcgctgtg ggccgtcacc ggcaacacgg acgtgccggg
     3721 cggcaacatc atcatccgca acgcgttcga gcagaacgtg tcgtacaact acggatacca
     3781 gacccttccc gccgaggtgc aggccaagcg catcggcgcg gagtacccgc tcatgtccaa
     3841 ggccgggttc agctccacgg cccactcgga ctccatcctg caggccatcg agaccggcaa
     3901 gccgtatccc atccgcatgc tctggctttc ctccacgaac cctatcgcca acatggcgtc
     3961 cgacgcgccg cgcgtctacc gggcggtgcg caccctcgac ttcgtcgtgg tgtccgaccc
     4021 gttcctcacc cccacggccg tggcgttcgc cgacgtgttc ctgccggcgt ccatgagctg
     4081 cgagcgcaac acgcagcgcg tatggtgggt gccgctgcgc ggcatgaaga aggtgacgca
     4141 gttcgccgac gtgcgttccg acgacaccat cgtcacggac ctcggacgcc gcctgcatcc
     4201 cgagaacttc ccctggaccg atgacatcgg ctggacgaac gatatcctcg tcaacgacac
     4261 gccaggttac gacggcgact tcgagcagct gtgcaaggac gtgtacgtct atcccgcgtt
     4321 cgagtaccgc aagcacgaga agggcctgct gcgccccgac gggcagcccg gtttcaacac
     4381 gccgtccggc cgcatcgagc tctacaacag catcttcgcg ctgtggggct acgacccgct
     4441 gccgcagtgg gaggagccat cctgctcgcc gtacgcctcg ccggagctgt acgagcagta
     4501 ccccttcgtg ctgacgacgg gcgcgcgctc ggtggagttc ttccattccg agcatcgcca
     4561 gctggagacg agccgcgagt tccaccccga tccgctgttc gagatgagcc cgaaggccgc
     4621 cgaggccgca gggctgtccg agggggattg gtgctggctg gagaaccatc gcggccgctg
     4681 ccgccagaag ctgcacgtga acccctcgct cgacgaccgc gtggtgcgcg ccgagcacgg
     4741 ctggtggttc ccggagcagg aggccgcgga acctgtcctg ttcggagtgt tcgactccaa
     4801 catcaacaac ctgctgccgc agtgcgagaa cgggccgacc ggttacggtg ccccgtacaa
     4861 gaaccagctg tgccgtgtgt acgcctgcac ggaggagaac agcgccgagc tgcccacggc
     4921 atgcgtgacg aggaaggacg ggtactcgaa atgagcgcta acgcattgat gatcgactac
     4981 gggttctgca cgggctgcca cgcctgcgag gtggcctgca agaagcatct gggcctgccc
     5041 gcgggccagc acggcatccg cgtgctccag gacggccccc gcgagctcgc gggcggaggg
     5101 tgggagtaca acttcctgcc catgcccacg tcgctctgcg acctgtgcgc ctcccgcctg
     5161 gccgagggca aggacgccgc ctgcgtccac cactgcccgg cccactgcat ggcgctcggg
     5221 ccggccgagg agctggcggc gagggctgcc gagaagacgc ggtgcgtggt cttctcctag
     5281 actagtcgac tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagaga
     5341 gagaatataa aaagccagat tattaatccg gcttttttat tatttaattc ctgttataaa
     5401 aaaaggatca attttgaact ctctcccaaa gttgatccct taacgatccc tgcaggagaa
     5461 ggccatcctg acggatggcc tttttgaagc tttctagaat cgggggcccc cggggggatt
     5521 ctagaacaat ggaagtcctc gtctcgccct cgaattagcc cgcttaatga gcgggctttt
     5581 tttgaattaa ttctcgcgag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     5641 cgcaacgcaa ttaatgtaag ttagcgcgaa ttgcaagctg gccgacgcgc tgggctacgt
     5701 cttgctggcg ttcgggagca gaagagcata catctggaag caaagccagg aaagcggcct
     5761 atggagctgt gcggcagcgc tcagtaggca atttttcaaa atattgttaa gccttttctg
     5821 agcatggtat ttttcatggt attaccaatt agcaggaaaa taagccattg aatataaaag
     5881 ataaaaatgt cttgtttaca atagagtggg gggggtcagc ctgccgcctt gggccgggtg
     5941 atgtcgtact tgcccgccgc gaactcggtt accgtccagc ccagcgcgac cagctccggc
     6001 aacgcctcgc gcacccgctg gcggcgcttg cgcatggtcg aaccactggc ctctgacggc
     6061 cagacatagc cgcacaaggt atctatggaa gccttgccgg ttttgccggg gtcgatccag
     6121 ccacacagcc gctggtgcag caggcgggcg gtttcgctgt ccagcgcccg cacctcgtcc
     6181 atgctgatgc gcacatgctg gccgccaccc atgacggcct gcgcgatcaa ggggttcagg
     6241 gccacgtaca ggcgcccgtc cgcctcgtcg ctggcgtact ccgacagcag ccgaaacccc
     6301 tgccgcttgc ggccattctg ggcgatgatg gataccttcc aaaggcgctc gatgcagtcc
     6361 tgtatgtgct tgagcgcccc accactatcg acctctgccc cgatttcctt tgccagcgcc
     6421 cgatagctac ctttgaccac catggcatca gcggtgacgg cctcccactt gggttccagg
     6481 aacagccgga gctgccgtcc gccttcggtc ttgggttccg ggccaagcac taggccatta
     6541 ggcccagcca tggccaccag cccttgcagg atgcgcagat catcagcgcc cagcggctcc
     6601 gggccgctga actcgatccg cttgccgtcg ccgtagtcat acgtcacgtc cagcttgctg
     6661 cgcttgcgct cgccccgctt gagggcacgg aacaggccgg gggccagaca gtgcgccggg
     6721 tcgtgtcgga cgtggctgag gctgtgcttg ttcttaggct tcaccacggg gcaccccctt
     6781 gctcttgcgc tgcctctcca gcacggcggg cttgagcacc ccgccgtcat gccgcctgaa
     6841 ccaccgatca gcgaacggtg cgccatagtt ggccttgctc acaccgaagc ggacgaagaa
     6901 ccggcgctgg tcgtcgtcca caccccattc ctcggcctcg gcgctggtca tgctcgacag
     6961 gtaggactgc cagcggatgt tatcgaccag taccgagctg ccccggctgg cctgctgctg
     7021 gtcgcctgcg cccatcatgg ccgcgccctt gctggcatgg tgcaggaaca cgatagagca
     7081 cccggtatcg gcggcgatgg cctccatgcg accgatgacc tgggccatgg ggccgctggc
     7141 gttttcttcc tcgatgtgga accggcgcag cgtgtccagc accatcaggc ggcggccctc
     7201 ggcggcgcgc ttgaggccgt cgaaccactc cggggccatg atgttgggca ggctgccgat
     7261 cagcggctgg atcagcaggc cgtcagccac ggcttgccgt tcctcggcgc tgaggtgcgc
     7321 cccaagggcg tgcaggcggt gatgaatggc ggtgggcggg tcttcggcgg gcaggtagat
     7381 caccgggccg gtgggcagtt cgcccacctc cagcagatcc ggcccgcctg caatctgtgc
     7441 ggccagttgc agggccagca tggatttacc ggcaccaccg ggcgacacca gcgccccgac
     7501 cgtaccggcc accatgttgg gcaaaacgta gtccagcggt ggcggcgctg ctgcgaacgc
     7561 ctccagaata ttgataggct tatgggtagc cattgattgc ctcctttgca ggcagttggt
     7621 ggttaggcgc tggcggggtc actacccccg ccctgcgccg ctctgagttc ttccaggcac
     7681 tcgcgcagcg cctcgtattc gtcgtcggtc agccagaact tgcgctgacg catccctttg
     7741 gccttcatgc gctcggcata tcgcgcttgg cgtacagcgt cagggctggc cagcaggtcg
     7801 ccggtctgct tgtccttttg gtctttcata tcagtcaccg agaaacttgc cggggccgaa
     7861 aggcttgtct tcgcggaaca aggacaaggt gcagccgtca aggttaaggc tggccatatc
     7921 agcgactgaa aagcggccag cctcggcctt gtttgacgta taaccaaagc caccgggcaa
     7981 ccaatagccc ttgtcacttt tgatcaggta gaccgaccct gaagcgcttt tttcgtattc
     8041 cataaaaccc ccttctgtgc gtgagtactc atagtataac aggcgtgagt accaacgcaa
     8101 gcactacatg ctgaaatctg gcccgcccct gtccatgcct cgctggcggg gtgccggtgc
     8161 ccgtgccagc tcggcccgcg caagctggac gctgggcaga cccatgacct tgctgacggt
     8221 gcgctcgatg taatccgctt cgtggccggg cttgcgctct gccagcgctg ggctggcctc
     8281 ggccatggcc ttgccgattt cctcggcact gcggccccgg ctggccagct tctgcgcggc
     8341 gataaagtcg cacttgctga ggtcatcacc gaagcgcttg accagcccgg ccatctcgct
     8401 gcggtactcg tccagcgccg tgcgccggtg gcggctaagc tgccgctcgg acagttcgag
     8461 gctggccagc ctgcgggcct tctcctgctg ccgctgggcc tgctcgatct gctggccagc
     8521 ctgctgcacc agcgccgggc cagcggtggc ggtcttgccc ttggattcac gcagcagcac
     8581 ccacggctga taaccggcgc gggtggtgtg cttgtccttg cggttggtga agcccgccaa
     8641 gcggccatag tggcggctgt cggcgctggc cgggtcggcg tcgtactcgc tggccagcgt
     8701 ccgggcaatc tgcccccgaa gttcaccgcc tgcggcgtcg gccaccttga cccatgcctg
     8761 atagttcttc gggctggttt ccactaccag ggcaggctcc cggccctcgg ctttcatgtc
     8821 atccaggtca aactcgctga ggtcgtccac cagcaccaga ccatgccgct cctgctcggc
     8881 gggcctgata tacacgtcat tgccctgggc attcatccgc ttgagccatg gcgtgttctg
     8941 gagcacttcg gcggctgacc attcccggtt catcatctgg ccggtggtgg cgtccctgac
     9001 gccgatatcg aagcgctcac agcccatggc cttgagctgt cggcctatgg cctgcaaagt
     9061 cctgtcgttc ttcatcgggc caccaagcgc agccagatcg agccgtcctc ggttgtcagt
     9121 ggcgtcaggt cgagcaagag caacgatgcg atcagcagca ccaccgtagg catcatggaa
     9181 gccagcatca cggttagcca tagcttccag tgccaccccc gcgacgcgct ccgggcgctc
     9241 tgcgcggcgc tgctcacctc ggcggctacc tcccgcaact ctttggccag ctccacccat
     9301 gccgcccctg tctggcgctg ggctttcagc cactccgccg cctgcgcctc gctggcctgc
     9361 tgggtctggc tcatgacctg ccgggcttcg tcggccagtg tcgccatgct ctgggccagc
     9421 ggttcgatct gctccgctaa ctcgttgatg cctctggatt tcttcactct gtcgattgcg
     9481 ttcatggtct attgcctccc ggtattcctg taagtcgatg atctgggcgt tggcggtgtc
     9541 gatgttcagg gccacgtctg cccggtcggt gcggatgccc cggccttcca tctccaccac
     9601 gttcggcccc aggtgaacac cgggcaggcg ctcgatgccc tgcgcctcaa gtgttctgtg
     9661 gtcaatgcgg gcgtcgtggc ctgcccgctc taatgcccgg ttggcatggt cggcccatgc
     9721 ctcgcgggtc tgctcaagcc atgccttggg cttgagcgct tcggtcttct gtgccccgcc
     9781 cttctccggg gtcttgccgt tgtaccgctt gaaccactga gcggcgggcc gctcgatgcc
     9841 gtcattgatc cgctcggaga tcatcaggtg gcagtgcggg ttctcgccgc caccggcatg
     9901 gatggccagc gtatacggca ggcgctcggc accggtcagg tgctgggcga actcggacgc
     9961 cagcgccttc tgctggtcga gggtcagctc gaccggcagg gcaaattcga cctccttgaa
    10021 cagccgccca ttggcgcgtt catacaggtc ggcagcatcc cagtagtcgg cgggccgctc
    10081 gacgaactcc ggcatgtgcc cggattcggc gtgcaagact tcatccatgt cgcgggcata
    10141 cttgccttcg cgctggatga agtcggcctt ggccctggcc gattggccgc ccgacctgct
    10201 gccggttttc gccgtaaggt gataaatcgc catgctgcct cgctgttgct tttgcttttc
    10261 ggctccatgc aatggccctc ggagagcgca ccgcccgaag ggtggccgtt aggccagttt
    10321 ctcgaagaga aaccggtaag tgcgccctcc cctacaaagt agggtcggga ttgccgccgc
    10381 tgtgcctcca tgatagccta cgagacagca cattaacaat ggggtgtcaa gatggttaag
    10441 gggagcaaca aggcggcgga tcggctggcc aagctcgaag aacaacgagc gcgaatcaat
    10501 gccgaaattc agcgggtgcg ggcaagggaa cagcagcaag agcgcaagaa cgaaacaagg
    10561 cgcaaggtgc tggtgggggc catgattttg gccaaggtga acagcagcga gtggccggag
    10621 gatcggctca tggcggcaat ggatgcgtac cttgaacgcg accacgaccg cgccttgttc
    10681 ggtctgccgc cacgccagaa ggatgagccg ggctgaatga tcgaccgaga caggccctgc
    10741 ggggctgcac acgcgccccc acccttcggg tagggggaaa ggccgctaaa gcggctaaaa
    10801 gcgctccagc gtatttctgc ggggtttggt gtggggttta gcgggctttg cccgcctttc
    10861 cccctgccgc gcagcggtgg ggcggtgtgt agcctagcgc agcgaataga ccagctatcc
    10921 ggcctctggc cgggcatatt gggcaagggc agcagcgccc cacaagggcg ctgataaccg
    10981 cgcctagtgg attattctta gataatcatg gatggatttt tccaacaccc cgccagcccc
    11041 cgcccctgct gggtttgcag gtttgggggc gtgacagtta ttgcaggggt tcgtgacagt
    11101 tattgcaggg gggcgtgaca gttattgcag gggttcgtga cagttagtac gggagtgacg
    11161 ggcactggct ggcaatgtct agcaacggca ggcatttcgg ctgagggtaa aagaactttc
    11221 cgctaagcga tagactgtat gtaaacacag tattgcaagg acgcggaaca tgcctcatgt
    11281 ggcggccctc gcgagaatta attcagataa aaaaaatcct tagctttcgc taaggatgat
    11341 ttctagcgat gaccctgctg attggttcgc tgaccgtaga ctggttggct tggtttcatc
    11401 agccatccgc ttgccctcat ctgttacgcc ggcggtagcc ggccagcctc gcagagcagg
    11461 attcccgttg agcaccgcca ggtgcgaata agggacagtg aagaaggaac acccgctcgc
    11521 gggtgggcct acttcaccta tcctgcccgg ctgacgccgt tggatacacc aaggaaagtc
    11581 tacacgaacc ctttggcaaa atcctgtata tcgtgcgaca tcgcgcgcgc gcgcgcggat
    11641 gctagcggcg cgccaaaaaa aagcccgctc atta
//
