LOCUS       sequence-463817-       11789 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses His-tagged Gordonibacter pamelaeae 3C (–)-Eddh with a 
            cumate inducible promoter in Gordonibacter urolithinfaciens DSM 
            27213 for protein purification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11789)
  AUTHORS   Dong X, Bae M, Le C, Aguilar Ramos MA, Balskus EP
  TITLE     Enantiocomplementary Gut Bacterial Enzymes Metabolize Dietary 
            Polyphenols.
  JOURNAL   J Am Chem Soc. 2025 Mar 5;147(9):7231-7244. doi: 
            10.1021/jacs.4c09892. Epub 2025 Feb 24.
  PUBMED    39993729
REFERENCE   2  (bases 1 to 11789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.4c09892"; journalName: "J Am Chem Soc"; date: 
            "2025-03-5- 5"; volume: "147"; issue: "9"; pages: "7231-7244"
COMMENT     Sequence Label: pXD80ENSF25-Pct3-(–)-Eddh(nHis6)(G3C)
FEATURES             Location/Qualifiers
     source          1..11789
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(19..813)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     primer_bind     1300..1318
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    1475..1494
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     CDS             complement(1497..2108)
                     /codon_start=1
                     /gene="cymR from Pseudomonas putida"
                     /product="cumate repressor (Mullick et al., 2006)"
                     /label=CymR
                     /translation="MSPKRRTQAERAMETQGKLIAAALGVLREKGYAGFRIADVPGAAG
                     VSRGAQSHHFPTKLELLLATFEWLYEQITERSRARLAKLKPEDDVIQQMLDDAAEFFLD
                     DDFSISLDLIVAADRDPALREGIQRTVERNRFVVEDMWLGVLVSRGLSRDDAEDILWLI
                     FNSVRGLAVRSLWQKDKERFERVRNSTLEIARERYAKFKR"
     protein_bind    2512..2539
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P2 operator sequence from the p-cmt 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     CDS             2600..2617
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      5413..5440
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      5572..5599
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(6031..6882)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(6869..7708)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(8219..9190)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELSERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     oriT            10429..10516
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     rep_origin      10858..11252
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     terminator      complement(11415..11458)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     oriT            11554..11663
                     /note="incP origin of transfer"
ORIGIN
        1 ggcgggctgg ccggccttct aaaacaattc atccagtaaa atataatatt ttattttctc
       61 ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg acatactgtt cttccccgat
      121 atcctccctg atcgaccgga cgcagaaggc aatgtcatac cacttgtccg ccctgccgct
      181 tctcccaaga tcaataaagc cacttacttt gccatctttc acaaagatgt tgctgtctcc
      241 caggtcgccg tgggaaaaga caagttcctc ttcgggcttt tccgtcttta aaaaatcata
      301 cagctcgcgc ggatctttaa atggagtgtc ttcttcccag ttttcgcaat ccacatcggc
      361 cagatcgtta ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc tattcgtata
      421 gggacaatcc gatatgtcga tggagtgaaa gagcctgatg cactccgcat acagctcgat
      481 aatcttttca gggctttgtt catcttcata ctcttccgag caaaggacgc catcggcctc
      541 actcatgagc agattgctcc agccatcatg ccgttcaaag tgcaggacct ttggaacagg
      601 cagctttcct tccagccata gcatcatgtc cttttcccgt tccacatcat aggtggtccc
      661 tttataccgg ctgtccgtca tttttaaata taggttttca ttttctccca ccagcttata
      721 taccttagca ggagacattc cttccgtatc ttttacgcag cggtattttt cgatcagttt
      781 tttcaattcc ggtgatattc tcattttagc catgatgttt aactttgttt tagggcgact
      841 gccctgctgc gtaacatcgt tgctgctcca taacatcaaa catcgaccca cggcgtaacg
      901 cgcttgctgc ttggatgccc gaggcataga ctgtacccca aaaaaacagt cataacaagc
      961 catgaaaacc gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt
     1021 gcgtgagcgc atacgctact tgcattacag cttacgaacc gaacaggctt atgtccactg
     1081 ggttcgtgcc ttcatccgtt tccacggtgt gcgtcacccg gcaaccttgg gcagcagcga
     1141 agtcgaggca tttctgtcct ggctggcgaa cgagcgcaag gtttcggtct ccacgcattc
     1201 gaaaccggta tcccgccgcc gccgccggat accggtttcg aattgagatt gccacctgac
     1261 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     1321 tttcgtcttc aaggcgaaaa aaccccgccg aagcggggtt ttttgcgaaa tataatgacc
     1381 ctcttgataa cccaagaggg cattttttag gaacggttat ttgattatta atccggcggc
     1441 aactgaaacg attcggatcc tgtattacta ttctgaattc gcggccgctt ctagatctag
     1501 cgcttgaatt tcgcgtaccg ctctcgcgca atttcgagtg tcgagttcct gacacgctca
     1561 aagcgttctt tgtccttctg ccataggcta cgaacagcaa gcccacgcac cgaattgaat
     1621 atcaaccaaa ggatatcttc tgcatcatca cgcgaaagac cacggctcac cagaacacca
     1681 agccacatat cctcgacgac aaaccgattc ctctctaccg tgcgctgaat accctcgcgt
     1741 aacgctgggt cccggtcggc agccacaatc aaatcaaggc tgatagagaa gtcatcgtcg
     1801 aggaaaaatt cggcggcgtc gtccagcatt tgctggatga cgtcatcctc tggcttcaat
     1861 ttcgctaatc gagcccgact gcgttcggtg atctgttcgt aaagccattc aaaagtggca
     1921 agcagaagct caagctttgt cgggaaatga tggctctgcg ctcctctgga gacaccagca
     1981 gcaccgggca catctgcgat ccggaatccc gcgtaacctt tttcccgtaa aacccccagg
     2041 gccgctgcaa tcaacttgcc ctgggtctcc attgcgcgct ctgcctgtgt tcttctcttt
     2101 ggactcatga tcaccagcga cctgctttcg ttcgtccggg catcacccgg gttactatct
     2161 agtatcaata actagattat tggttgaaac gtactatgtc aagcagttct aaagattgac
     2221 tccaaacggt cgccttgcta tgctcgtttc gtttcctccg cacgcgcgcg aaaggctcct
     2281 tcacggagcc aatgtacgca aaaaaccccg cttcagcggg gttttttcgc caaaaaaaac
     2341 cccgccctgt caggggcggg gttttttttt ttagggccct actgactgtg agcccgctcg
     2401 atcaacggat cggtaaaata cgggcgaaaa agagaatccc gagctttttt gttgccgcat
     2461 agcgtatagt aagcatgtgc cctcgaggtg agggcgagtg ttgcggccag taacaaacag
     2521 acaatctggt ctgtttgtat tatgcagcac acagtgttac ctcctcgggg acgaggagag
     2581 gaaagaggat ttaaaaatgc atcaccatca ccatcacgac tacgacatcc cgactaccga
     2641 gtacaacaac gagtacaacc ttgacctgta cccgaagaag ctgcactgga aggagggcga
     2701 cctcactgcc acgcgcacga ccatgtggtc gggcccgggc tgccacgagg gatgccaggt
     2761 gatcttctac acggacgacg acggcaagct cgtgaaggtt gagggcgatc cgaacagccc
     2821 gttcaaccag gggcgcctct gcatgcgctg cctggagctg cccgagctcg tgaaccacga
     2881 ggagcgcctg cgctacccca tgaagcgcgc cgggaagcgc ggcgaggaca agtgggagcg
     2941 cgtgagctgg gacaaggcct acgatatcat cgaggaaaag gtccgcgcgt tccaggagaa
     3001 ggacggcccc gagtccatca tctccatgat cggcaccggc cgcaacgtct cgcaggtcat
     3061 cgcgcacaac cagtacgcga acttcggcgg ccccgacctc acgctctgct tcctgtcggg
     3121 cgactcgtgc atgctgccgc gcacggcgct ctgctacgtg gtcatgggca accagtgggt
     3181 ggccgacatg agccagttcc gccccgaccg ctacgagaac gaccccgagt ggcagatccc
     3241 cgagtgcatc gtcatctggg gcacgaaccc ggtcgtgtgc aactccgacg ccttcctcgg
     3301 ccactggatc gtggaggcca tgaagcgcgg cagcgagctc gtgaccatcg acccgcagct
     3361 cacgtggatc gcctccaagt ccaagtactg gctgcgcctg cgccccggca ccgacgcggc
     3421 gctcgccctc ggcatgatga acgtcatcat cggcgaggac ctcgtggacc acgacttcat
     3481 cgacaagtgg acctacggct ttgaggcgct tgccgagcgc gtgaaggact atccggttga
     3541 gaaggtggcc gagatctgct gggtcgagcc cgagctcatc gtggaggccg cgcgcttcta
     3601 cgcgaaggcc cacccctcca ccatccagtg gggcctcgcc gtggacatgt cgaagatcgg
     3661 cacccctacg gcccacgcca tcgcctgctt ggccggcatc acgggcaaca tcgacaaccc
     3721 cggcggcaac atcctcatcg accaggcctg cggcctggag ttcggctaca actccggcat
     3781 cgagtacctc aaagagggca tggtggagaa gcgcctgggc aactccaagt acaacctgaa
     3841 gaagtacggc ttcacggcgt cctcccagtc cgacgccatc ctcgaggcgt gcgagaccgg
     3901 ccttgacgag aagggcgacc cgcgcccggt gaacatgctg tggatccagt cctccaaccc
     3961 catcacgaac atgggccagg acgcgccgcg catctaccgc gccatggaga aggtaccgtt
     4021 catctgcgtg gtcgacctgt tcaagacgcc gttcatcgtg gcctgcgccg acctcgtgct
     4081 gccctgcgcc atgtccaacg agcgcgactg catccgcgtg tggttcgacc cggtccgctc
     4141 gctcacgaag tgctcgagct tctacgaggc gaaggaagac gagcagatca tgatcgacct
     4201 ggggcaccgt ttggcacccg agagctggcc cgagtgggtc aaggagccgc gcgactacat
     4261 gaactggcgc ctccaggtgg gcaactgcgg cttcaccatg gaggagctgg agaacgacca
     4321 tgccggcatg atctacaacg acttccgcta ctacaagtac gagaagggcc tgctgcgtcc
     4381 cgacggccag cccggcttca tgacccctac gggccgctac gagttctaca tcagcctgtt
     4441 cgacagctgg ggcgtggacg cgctgccgtt ctacgaggag cctcccacct cgccggtctc
     4501 cacgccggag ctggccgagg agtacccgct cgtgctcacc acgggcaagc gctccttcga
     4561 gttcttccac tccgagtggc gccaggcgaa cacgacgagc cgcgagctgc acccggtgcc
     4621 ctacttcgac atccatcccg acacggcggc gaaatacggc gtggccgagg gccagtggac
     4681 ctggatcgag aaccagatgg gcaagtgccg ccaggtggcg cgcttcaacg acacgctcga
     4741 tccccgcgtc atctccaccg agcacggctg gtggttcccc gagcaggagg cggccgagcc
     4801 gagcctgttc ggcgtgttcg actgcaaccc gaacaacctc attcccatgt gcgagaacgg
     4861 cccgtccggc tacggcgcgc ccatcaagtg cggcatggcg aaggtgtacc cctgcacgcc
     4921 ggacaacatg aggcccgagg accagcccac ctaccaggtg acggccacga acggctacac
     4981 gcacggcccc tcgcacaaga gcgacgagcc tagcttctac gaccagaaca agtaaggggg
     5041 agacatcatg gcacaaaccg gcgaatacgg ccttctgatc gactaccagt actgctcggg
     5101 ctgccacgcc tgcgaggtgg cctgcaagaa ggagcatgac ctgcccaagg gcgacttcgg
     5161 catcaagatc gtgcaggacg gcccgcgcca gtgctccgac ggcgtgtggg agtacgacta
     5221 cctgcccgtg cccacgcacc tgtgcgacct gtgcgccgtg cgcgtggagg ccggcaagct
     5281 tcccacctgc gtgcaccact gccaggccgg cgtcatggtc tacggcacgc tggaggagct
     5341 cgccgccaag gctaaggcgg ccgcgaagga caagatggtg atcttcgcga agtagactag
     5401 tcgactcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact agagagagaa
     5461 tataaaaagc cagattatta atccggcttt tttattattt aattcctgtt ataaaaaaag
     5521 gatcaatttt gaactctctc ccaaagttga tcccttaacg atccctgcag gagaaggcca
     5581 tcctgacgga tggccttttt gaagctttct agaatcgggg gcccccgggg ggattctaga
     5641 acaatggaag tcctcgtctc gccctcgaat tagcccgctt aatgagcggg ctttttttga
     5701 attaattctc gcgagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     5761 cgcaattaat gtaagttagc gcgaattgca agctggccga cgcgctgggc tacgtcttgc
     5821 tggcgttcgg gagcagaaga gcatacatct ggaagcaaag ccaggaaagc ggcctatgga
     5881 gctgtgcggc agcgctcagt aggcaatttt tcaaaatatt gttaagcctt ttctgagcat
     5941 ggtatttttc atggtattac caattagcag gaaaataagc cattgaatat aaaagataaa
     6001 aatgtcttgt ttacaataga gtgggggggg tcagcctgcc gccttgggcc gggtgatgtc
     6061 gtacttgccc gccgcgaact cggttaccgt ccagcccagc gcgaccagct ccggcaacgc
     6121 ctcgcgcacc cgctggcggc gcttgcgcat ggtcgaacca ctggcctctg acggccagac
     6181 atagccgcac aaggtatcta tggaagcctt gccggttttg ccggggtcga tccagccaca
     6241 cagccgctgg tgcagcaggc gggcggtttc gctgtccagc gcccgcacct cgtccatgct
     6301 gatgcgcaca tgctggccgc cacccatgac ggcctgcgcg atcaaggggt tcagggccac
     6361 gtacaggcgc ccgtccgcct cgtcgctggc gtactccgac agcagccgaa acccctgccg
     6421 cttgcggcca ttctgggcga tgatggatac cttccaaagg cgctcgatgc agtcctgtat
     6481 gtgcttgagc gccccaccac tatcgacctc tgccccgatt tcctttgcca gcgcccgata
     6541 gctacctttg accaccatgg catcagcggt gacggcctcc cacttgggtt ccaggaacag
     6601 ccggagctgc cgtccgcctt cggtcttggg ttccgggcca agcactaggc cattaggccc
     6661 agccatggcc accagccctt gcaggatgcg cagatcatca gcgcccagcg gctccgggcc
     6721 gctgaactcg atccgcttgc cgtcgccgta gtcatacgtc acgtccagct tgctgcgctt
     6781 gcgctcgccc cgcttgaggg cacggaacag gccgggggcc agacagtgcg ccgggtcgtg
     6841 tcggacgtgg ctgaggctgt gcttgttctt aggcttcacc acggggcacc cccttgctct
     6901 tgcgctgcct ctccagcacg gcgggcttga gcaccccgcc gtcatgccgc ctgaaccacc
     6961 gatcagcgaa cggtgcgcca tagttggcct tgctcacacc gaagcggacg aagaaccggc
     7021 gctggtcgtc gtccacaccc cattcctcgg cctcggcgct ggtcatgctc gacaggtagg
     7081 actgccagcg gatgttatcg accagtaccg agctgccccg gctggcctgc tgctggtcgc
     7141 ctgcgcccat catggccgcg cccttgctgg catggtgcag gaacacgata gagcacccgg
     7201 tatcggcggc gatggcctcc atgcgaccga tgacctgggc catggggccg ctggcgtttt
     7261 cttcctcgat gtggaaccgg cgcagcgtgt ccagcaccat caggcggcgg ccctcggcgg
     7321 cgcgcttgag gccgtcgaac cactccgggg ccatgatgtt gggcaggctg ccgatcagcg
     7381 gctggatcag caggccgtca gccacggctt gccgttcctc ggcgctgagg tgcgccccaa
     7441 gggcgtgcag gcggtgatga atggcggtgg gcgggtcttc ggcgggcagg tagatcaccg
     7501 ggccggtggg cagttcgccc acctccagca gatccggccc gcctgcaatc tgtgcggcca
     7561 gttgcagggc cagcatggat ttaccggcac caccgggcga caccagcgcc ccgaccgtac
     7621 cggccaccat gttgggcaaa acgtagtcca gcggtggcgg cgctgctgcg aacgcctcca
     7681 gaatattgat aggcttatgg gtagccattg attgcctcct ttgcaggcag ttggtggtta
     7741 ggcgctggcg gggtcactac ccccgccctg cgccgctctg agttcttcca ggcactcgcg
     7801 cagcgcctcg tattcgtcgt cggtcagcca gaacttgcgc tgacgcatcc ctttggcctt
     7861 catgcgctcg gcatatcgcg cttggcgtac agcgtcaggg ctggccagca ggtcgccggt
     7921 ctgcttgtcc ttttggtctt tcatatcagt caccgagaaa cttgccgggg ccgaaaggct
     7981 tgtcttcgcg gaacaaggac aaggtgcagc cgtcaaggtt aaggctggcc atatcagcga
     8041 ctgaaaagcg gccagcctcg gccttgtttg acgtataacc aaagccaccg ggcaaccaat
     8101 agcccttgtc acttttgatc aggtagaccg accctgaagc gcttttttcg tattccataa
     8161 aacccccttc tgtgcgtgag tactcatagt ataacaggcg tgagtaccaa cgcaagcact
     8221 acatgctgaa atctggcccg cccctgtcca tgcctcgctg gcggggtgcc ggtgcccgtg
     8281 ccagctcggc ccgcgcaagc tggacgctgg gcagacccat gaccttgctg acggtgcgct
     8341 cgatgtaatc cgcttcgtgg ccgggcttgc gctctgccag cgctgggctg gcctcggcca
     8401 tggccttgcc gatttcctcg gcactgcggc cccggctggc cagcttctgc gcggcgataa
     8461 agtcgcactt gctgaggtca tcaccgaagc gcttgaccag cccggccatc tcgctgcggt
     8521 actcgtccag cgccgtgcgc cggtggcggc taagctgccg ctcggacagt tcgaggctgg
     8581 ccagcctgcg ggccttctcc tgctgccgct gggcctgctc gatctgctgg ccagcctgct
     8641 gcaccagcgc cgggccagcg gtggcggtct tgcccttgga ttcacgcagc agcacccacg
     8701 gctgataacc ggcgcgggtg gtgtgcttgt ccttgcggtt ggtgaagccc gccaagcggc
     8761 catagtggcg gctgtcggcg ctggccgggt cggcgtcgta ctcgctggcc agcgtccggg
     8821 caatctgccc ccgaagttca ccgcctgcgg cgtcggccac cttgacccat gcctgatagt
     8881 tcttcgggct ggtttccact accagggcag gctcccggcc ctcggctttc atgtcatcca
     8941 ggtcaaactc gctgaggtcg tccaccagca ccagaccatg ccgctcctgc tcggcgggcc
     9001 tgatatacac gtcattgccc tgggcattca tccgcttgag ccatggcgtg ttctggagca
     9061 cttcggcggc tgaccattcc cggttcatca tctggccggt ggtggcgtcc ctgacgccga
     9121 tatcgaagcg ctcacagccc atggccttga gctgtcggcc tatggcctgc aaagtcctgt
     9181 cgttcttcat cgggccacca agcgcagcca gatcgagccg tcctcggttg tcagtggcgt
     9241 caggtcgagc aagagcaacg atgcgatcag cagcaccacc gtaggcatca tggaagccag
     9301 catcacggtt agccatagct tccagtgcca cccccgcgac gcgctccggg cgctctgcgc
     9361 ggcgctgctc acctcggcgg ctacctcccg caactctttg gccagctcca cccatgccgc
     9421 ccctgtctgg cgctgggctt tcagccactc cgccgcctgc gcctcgctgg cctgctgggt
     9481 ctggctcatg acctgccggg cttcgtcggc cagtgtcgcc atgctctggg ccagcggttc
     9541 gatctgctcc gctaactcgt tgatgcctct ggatttcttc actctgtcga ttgcgttcat
     9601 ggtctattgc ctcccggtat tcctgtaagt cgatgatctg ggcgttggcg gtgtcgatgt
     9661 tcagggccac gtctgcccgg tcggtgcgga tgccccggcc ttccatctcc accacgttcg
     9721 gccccaggtg aacaccgggc aggcgctcga tgccctgcgc ctcaagtgtt ctgtggtcaa
     9781 tgcgggcgtc gtggcctgcc cgctctaatg cccggttggc atggtcggcc catgcctcgc
     9841 gggtctgctc aagccatgcc ttgggcttga gcgcttcggt cttctgtgcc ccgcccttct
     9901 ccggggtctt gccgttgtac cgcttgaacc actgagcggc gggccgctcg atgccgtcat
     9961 tgatccgctc ggagatcatc aggtggcagt gcgggttctc gccgccaccg gcatggatgg
    10021 ccagcgtata cggcaggcgc tcggcaccgg tcaggtgctg ggcgaactcg gacgccagcg
    10081 ccttctgctg gtcgagggtc agctcgaccg gcagggcaaa ttcgacctcc ttgaacagcc
    10141 gcccattggc gcgttcatac aggtcggcag catcccagta gtcggcgggc cgctcgacga
    10201 actccggcat gtgcccggat tcggcgtgca agacttcatc catgtcgcgg gcatacttgc
    10261 cttcgcgctg gatgaagtcg gccttggccc tggccgattg gccgcccgac ctgctgccgg
    10321 ttttcgccgt aaggtgataa atcgccatgc tgcctcgctg ttgcttttgc ttttcggctc
    10381 catgcaatgg ccctcggaga gcgcaccgcc cgaagggtgg ccgttaggcc agtttctcga
    10441 agagaaaccg gtaagtgcgc cctcccctac aaagtagggt cgggattgcc gccgctgtgc
    10501 ctccatgata gcctacgaga cagcacatta acaatggggt gtcaagatgg ttaaggggag
    10561 caacaaggcg gcggatcggc tggccaagct cgaagaacaa cgagcgcgaa tcaatgccga
    10621 aattcagcgg gtgcgggcaa gggaacagca gcaagagcgc aagaacgaaa caaggcgcaa
    10681 ggtgctggtg ggggccatga ttttggccaa ggtgaacagc agcgagtggc cggaggatcg
    10741 gctcatggcg gcaatggatg cgtaccttga acgcgaccac gaccgcgcct tgttcggtct
    10801 gccgccacgc cagaaggatg agccgggctg aatgatcgac cgagacaggc cctgcggggc
    10861 tgcacacgcg cccccaccct tcgggtaggg ggaaaggccg ctaaagcggc taaaagcgct
    10921 ccagcgtatt tctgcggggt ttggtgtggg gtttagcggg ctttgcccgc ctttccccct
    10981 gccgcgcagc ggtggggcgg tgtgtagcct agcgcagcga atagaccagc tatccggcct
    11041 ctggccgggc atattgggca agggcagcag cgccccacaa gggcgctgat aaccgcgcct
    11101 agtggattat tcttagataa tcatggatgg atttttccaa caccccgcca gcccccgccc
    11161 ctgctgggtt tgcaggtttg ggggcgtgac agttattgca ggggttcgtg acagttattg
    11221 caggggggcg tgacagttat tgcaggggtt cgtgacagtt agtacgggag tgacgggcac
    11281 tggctggcaa tgtctagcaa cggcaggcat ttcggctgag ggtaaaagaa ctttccgcta
    11341 agcgatagac tgtatgtaaa cacagtattg caaggacgcg gaacatgcct catgtggcgg
    11401 ccctcgcgag aattaattca gataaaaaaa atccttagct ttcgctaagg atgatttcta
    11461 gcgatgaccc tgctgattgg ttcgctgacc gtagactggt tggcttggtt tcatcagcca
    11521 tccgcttgcc ctcatctgtt acgccggcgg tagccggcca gcctcgcaga gcaggattcc
    11581 cgttgagcac cgccaggtgc gaataaggga cagtgaagaa ggaacacccg ctcgcgggtg
    11641 ggcctacttc acctatcctg cccggctgac gccgttggat acaccaagga aagtctacac
    11701 gaaccctttg gcaaaatcct gtatatcgtg cgacatcgcg cgcgcgcgcg cggatgctag
    11761 cggcgcgcca aaaaaaagcc cgctcatta
//
