LOCUS       sequence-463819-       13293 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Eggerthella lenta A2 Dadh in Eggerthella lenta DSM 2243.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13293)
  AUTHORS   Dong X, Bae M, Le C, Aguilar Ramos MA, Balskus EP
  TITLE     Enantiocomplementary Gut Bacterial Enzymes Metabolize Dietary 
            Polyphenols.
  JOURNAL   J Am Chem Soc. 2025 Mar 5;147(9):7231-7244. doi: 
            10.1021/jacs.4c09892. Epub 2025 Feb 24.
  PUBMED    39993729
REFERENCE   2  (bases 1 to 13293)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.4c09892"; journalName: "J Am Chem Soc"; date: 
            "2025-03-5- 5"; volume: "147"; issue: "9"; pages: "7231-7244"
COMMENT     Sequence Label: pXD70DA8-DadhA(cHis8)BC-DadRS-noAccs(ElA2)
FEATURES             Location/Qualifiers
     source          1..13293
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      24..95
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(276..864)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(356..375)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1035..1826,1827..1895))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1658..1677
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1896..2000)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2068..2086
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    2243..2262
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     CDS             7771..7794
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     terminator      9320..9347
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(9470..10264)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 agcttgctgg ggcttttctt tttcaaataa aacgaaaggc tcagtcgaaa gactgggcct
       61 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gaggagctca tgccggcggc
      121 acctcgctaa cggattcacc actccaagaa ttggagccaa tcaattcttg cggagaactg
      181 tgaatgcgca aaccaaccct tggcagaaca tatccatcgc gtccgccatc tccagcagcc
      241 gcacgcggcg catctcgggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
      301 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
      361 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
      421 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
      481 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
      541 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
      601 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
      661 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
      721 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
      781 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
      841 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
      901 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
      961 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     1021 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     1081 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     1141 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     1201 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     1261 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     1321 aatagtttgc gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt
     1381 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     1441 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     1501 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     1561 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     1621 cggcgaccga gttgctcttg cccggcgtca acacgggata ataccgcgcc acatagcaga
     1681 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     1741 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     1801 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     1861 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     1921 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     1981 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
     2041 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa
     2101 ggcgaaaaaa ccccgccgaa gcggggtttt ttgcgaaata taatgaccct cttgataacc
     2161 caagagggca ttttttagga acggttattt gattattaat ccggcggcaa ctgaaacgat
     2221 tcggatcctg tattactatt ctgaattcgc ggccgcttct agattcattc cgaaacggaa
     2281 tggatgaaat cgagcgcctc ctgcttgtcg cccacgccca gcttgcggta cacgctgccc
     2341 agcacgtcgg ccacctcgtc gcgcgtcagc gccagctcgc cctggatcgc ctcgaagccg
     2401 cgctcctcca cgtacaggat gagcgcgccc agctcgcgcc ggtcgagccc gcagcgctcg
     2461 gccgcgagcc ggcacttgca gcgaagcgcc gtgtcgcacg cggccgcagg cgagccgtcg
     2521 acggcacccc cgcccgccgc tgcgcccgcc gtcgcgcccg caacccccgc gcccgccgaa
     2581 ccctgcccgt cgcgcgccgc gcgcaccagc agctcggtgg cgcgcagcag cttctccatc
     2641 tgccgcgcct gggcgtacag cacgccgccc gcctcggtca ggctcacgcc gctgcggtcg
     2701 cgcacgaaca ggtcggcccc gaattcgcgc tccaacaagg ccacgtgctt gctcagcgtc
     2761 gactgcgtca gatgcagctc gcgcgcggcg gccgtgaagc tgccgcaatc ggccaccttc
     2821 gcgaactcgt gcagatacgc cgtctccatc gcctcagacc gcctcgtcct gcgcggccgt
     2881 ctcgtgggcc aggtcgatga gctcctggcg attgtccacg cccgtcttct ggtagatgtg
     2941 gcggatatgc gtgctcaccg tgcccgccga gatgaacagc gtctcctgga tgcgcgagat
     3001 ggtgcggccc tccagcagca gcggaagcac gtcggtctcg cgcggcgaca gggcgaagcg
     3061 ctccgcgatc agcgcgcacg gatccaccgg gtcggccggg tcgtcggcgg cctcttcggg
     3121 caccgtgccc atcaggctga cctcgccgat gccgattttg atgaggtcgg tctccgtgaa
     3181 caggaacaaa tgcgcgatga acagcagcga cacggccacc agcgtgacca gcgtgaggtc
     3241 gaacatgctg accccggtcg cctccagcga atgggcgaac agctggcctg cggtctcgcc
     3301 gccgtacagc gccacgaacc ccaggccgat cgtccgcgcc gggctcacgc ggaagctgga
     3361 cgccacgtcg atgcacacca cgcacagcac cagcacgaag cagtggtagc cgccgaagat
     3421 aagcgcgctc gaatacgtgt tgttgtcggc catgaacggc gtggacaagc atcccaagca
     3481 cagcatcagc atggagacgc ggtacgagaa gaacacgctg tcgcgctgca ggaacacggc
     3541 tatcaggcag atggccaacg ctccgatgaa gcccgccaga aacaggtacg tgccgaagat
     3601 gtcctgcacc tcgtaggttt tgaaccccga caccacgtcg aagaacccca tcaacatgcc
     3661 gatgagcacc gcgcccagaa gcgccttcgc gaacagggtc gccgtatggc gcgacaaagg
     3721 ctgctcgggc tcgcgcggca gctgcggcgt atcgggcgcg ctgcgcctga gagctgccgc
     3781 cgaaagcacg ggcatgacaa cgatgagcac gatgacgggt atcgccggcc ctacgatcag
     3841 caagaacccc acgacgaacg cgatgcacag cgacgaggca agctccacaa gcgccaagcg
     3901 gcctcccagc cccgactcga gctcgatcca gcacaagaac agcacggccg agcccgtgcc
     3961 ggtcatgacc gcgctcaggc ccagcaccag catgccgacg ggcgtggtag gatcggagaa
     4021 cggcgtgagc acgctgcccg cgctcatcat gatggccgat cccagcacga tggagcggtt
     4081 gccgctcacc cgctcgcgcc gcacgatgac ggagacgaac agcatggtca ccaccatggc
     4141 caccagcgat atgatcaggc tgacgtacag cgagccgcgc acgtccgacg agaagccgta
     4201 cggcacgatg ctgtagaaca tcatgagcat ccaaagccat tgcaaggccg cgcccgcgat
     4261 gcgcttgagg gaaaggctcg tccccaacgc ggtatcccat gcactccgaa gcttttccat
     4321 gccccacccc ctgtgagccc gtacataacg tcgctcgcgc cgacccctct gtcgacgggt
     4381 tcgattatac ggaaaagatg agcggccctc tttgaggaaa ctattctagt ttcagaataa
     4441 tttatgacct acggttaaag tttccccagt tcaaagcact atcgaatttt tcatgatcac
     4501 aagatcataa gaatttatga tgcggtcgaa cctgctttcc aacctttgga cgatgctctg
     4561 caccccctcg catccgtatg gtcggcccaa gcgacacggg gactttgcac cggttcggag
     4621 cccgaggctt cacccggcag cccccctccc cgatcgcacg aacgaagacg agcaagacga
     4681 gaacgaggag aaagaaggag gaggagtatg ggtaacctga ccatgtcacg acgcacgttc
     4741 gtcaagaccg ccgccatcac cggtgcggca gccgcggcgt tcggcgcttc gacgcacacc
     4801 gcgctggccg aagagacgta cagcagcgtg tccggcaacg acaccgtggc cgtgaaaacg
     4861 tgctgccgcg gctgcggcaa gatggagtgc ggcgtcaagg tcatcgtcca gaacgggcgg
     4921 gccatacgcg tcgagggcga cgagggagcg ttccagtcca tgggcaactg ctgcacgaag
     4981 tcgcagtcgt ccatccaggc ggcgtaccac cccgaccgtc tgcactatcc catgaagcgc
     5041 acgaacccga agggcgagga gcccggctgg cagcgcatct cgtgggacga ggccatgcag
     5101 tccatcgtgg acaacttcat ggacatcaag gcgaagcacg gcggcgaggc catcgcctgc
     5161 caggtgggca cgtcgcgcat ctggtgcatg cactcggagt ccatcctgaa gaacatgctg
     5221 gaaacgccga acaacgttga ggcctggcag atctgcaagg gcccgcgcca cttcgccacc
     5281 accatggtgt cgcagttcgc catgtcgtgg atggaaacca tcacgcgccc gaaagtgtac
     5341 gtgcagtggg gcggcgcgtc cgagctgtcc aactacgacg actcctgccg caccacggtg
     5401 gacgtggcat cgcgcgccga cgtgcacatc agcgtcgacc cccgcatggc caacatgggc
     5461 aaggaagccg actactggca gcacctgcgc cccggcaccg acggcgccct ggccctggcc
     5521 tggacgaacg tcatcatcga gaagaagctc tacgacgagc tgtacgtgaa gaagtggacg
     5581 aacgccccgt tcctcgtgtg cgaggacatg gagccctccg gcttccccac cgtgcgcacc
     5641 gacggttcct actgggacgt gaaaaccgcg ctgctcaagg aaagcgacat caaggagggc
     5701 ggcagcccct ataagttcct cgtgtacgac aacaactggg agaagctgaa ggccgagggc
     5761 gtcgagcacg agtacggcgc gttcacctgg ttcaacgccg accaggaagg cgtcatcgac
     5821 gagaccggcg gcttctggga gggcgagaac tacgactccg agaaggcgcg ccaaggccgc
     5881 gaggccgcgc aggacaacct gctgccgggc cagacgcagg gctggctgcc cgatcccatg
     5941 ccgttcgacc ccgccatcga ccccgcgctg gaaggcgagt tcgagatcac gctgaaggac
     6001 ggcaagaccg tgaaggtcaa gccggtgtgg gagcactaca aggcccgcgc cgccgagtac
     6061 aagcccgagg tggcggccga gatcaccggc atccccgcct ccgagatcga ggcggcggcc
     6121 acggcctacg gcacgcgcat cgacccgtcc accggctacg gcaacggcgg catccagtac
     6181 atgctggcgg tcgagcactt ctgcagcgcc atccagaact gccgcgcctt cgacaacctc
     6241 gtgggcatca ccggcaacat ggacaccccg ggcggcaacc gcggcccgac catcgtcccc
     6301 atcgacggcg acctccaggg cttcagcgcc tgggcccccg gcgccaccac cccgccggag
     6361 gaagtcaacc gcaagcagat cggcatcgac aagttcccgc ttctgggctg gtggcagtac
     6421 tggtgcgaca gccattcgct gtgggacgcc gtcatcacgg gcgaccccta cccggtgcgc
     6481 gccctctgga acgagtccgg caacttcatg agccagacga acaccacgcg cgcctgggag
     6541 gccctgtgct cgcttgactt ctacgtggac ctcaacctgt ggcacacgcc gcagaacgac
     6601 accgccgaca tcatcctgcc ggtggcccat tggatcgagc tcaactcgcc gcgcgccagc
     6661 caaggttccg ccggcgccat gggcgccacg gtcaagtgcg tgcagccgcc cgcggaagcc
     6721 aagtacgatc ccgagatcgt catggacctc gcccgccgca tgaactggaa gtggaccgac
     6781 gagccgggca acgagtggcc cgacatcaac tggcagctgg acgactccat caagctgctc
     6841 accgacgacg agctgaccta caccacgtgg cacgtcgaga acggcaagcc cacgttcgag
     6901 cgccacggcg ttccgatggc cgaggtcacg cccaagtaca agacgtggga cgagtacgtc
     6961 aaggccttcc aggagcacgg ctggtggcag gcgaaggaca tcgagccgcg caactggggc
     7021 acgtaccgcc gctaccagac cggcgcgatg cgcgcacgcg accgcgtgtg gggccgcctc
     7081 gactacacgg ccggcaaggg catcggcgac tggaagccgg gctggttcac cccgacgatg
     7141 aagcaggaga tctggtccac cgtcatggaa tcgcaccatc ccgaccatcc cgagtggagg
     7201 cttcccacct acaccgagcc gcctcacggc ccgaaggacg gcgaccgcat caaggagtac
     7261 ccgctgaccg ccaccaccgg ccgtcgcatc ccggtgtact tccactccga gcatcgccag
     7321 cttccctggt gccgcgagct gtggcccgtg ccgcgcgtgg agatcaaccc gaagacggcc
     7381 gccgagtacg gcatcgagca gggcgactgg gtgtggatcg aaaccgagtg gggcaagatc
     7441 cgcgaagtgg ctgacctgta ctacggcgtg aaggaagacg tcatcaacct cgagcacacg
     7501 tggtggtacc ccgaggtgaa ggacgccggc cacggctggc agttctccca ggtgaaccag
     7561 ctgatcgacc actacgccca ggacccgcac tccggcacgt ccaacctgcg cgcctaccag
     7621 gtgaagatct acaaggccac gcccgagaac tcgccgttca acaaccccgt gccgtgcgac
     7681 tccaccggca cgcccatcat ccatacctcc gacgaccccc gtctgaagga atggctgcct
     7741 acctacgaag ggagggaggc cgcactcgag catcaccatc accatcacca tcactgaacc
     7801 atgacgaagg ctatcatcac cgacctcaac cggtgcgtgg gctgcctggc gtgctcggtc
     7861 gcctgcaagg cggtcaacaa cgtgcccatc ggcagctact ggaacaaagt ggtgcgcgtg
     7921 gggccgaacc ccatcccggg cggatcaggg cagtaccccg acgtgtacat gtacttcctg
     7981 cccatcagct gccagcactg cgagaacccc gagtgcgtga aggtgtgccc caccgaggcg
     8041 tcgcacgtgg ccgaggacgg ctccatccag atcgacaagg agaagtgcat cggctgccag
     8101 ttctgcgtca tggcctgccc ctacggcgtc cgctacctca acgaggagga gaaggtcgtc
     8161 gagaagtgca cgctgtgcga gcagaagctg tcgcagggcg agctgcccca gtgcgtcagc
     8221 cagtgcggcg gcaacgcccg ctggatcggc gacaccgagg ccggctacga gtcgttcgag
     8281 ggctccgagg acccgctggg caagcgccgc aagatggtgg agttcctgga gccgttcacc
     8341 gacgccgacg tgcacaagct gcccgacgtg ggcaacaagc cgtcgtactc ctacatccta
     8401 cgcgaccatc actgggaagg aggtgatgag taatggaaac gcagtggcct ctcgtcatct
     8461 tcacgctgtt cgtatgcttg acctgcggca cgctgggcgg catgtccatc ctggcgctca
     8521 agggccaggg caggaacctg cagatgaccg cgctcatcac gtccgccgtc tcgctggtgg
     8581 taggcggcat cggagccttc ctgcacctcg agcactggga gcgcatcttc aacggcttcg
     8641 ggcacatcac gtcgggcatc acccaggagc tcatcggatg cgtggcgctg gccatcgtca
     8701 tcgtcgcatg gttcgtggtc cttcgaggcg gcaagcccgt tcccaaggcg ctggcctggg
     8761 cgaccctcgc ggttgccgtg ctcatgatgg tcgccaccgc gcactcctac ctcatgccgg
     8821 cccgtcccgc gtggggcctg gcgctcgtgg cgttctatct gggcaacgcg tgcctcctgg
     8881 gcgccgcggc cgtctggctg atctccatcc tgaagaagga cgaggccgtc gaggcgacgg
     8941 gcatccagct gacgttcatc gcggccctcg tgcagatcgt ggccgacgtg gtgttcgtca
     9001 tcgcctgcgc catggcgaag atcgcgcaat tcggctacta cgccgacccg acatcgatga
     9061 ccacggcccc cacgcacgtc gacagcctga tgaccgtcat ggtgtccggc gcgggagcgc
     9121 ccatgttctg gggctccatc gtctccgtga tcgtggctgc ggcctgcgcc ttcgtggcga
     9181 agaagaaggt gggcgcctcc aagacgctca tggtcgtcgc cgccatcggc gcgctggcca
     9241 cctccgtgat gtttcgcgtg ctgatctacc agctgggcta cccagtggtc ctgatgtact
     9301 aaactagtcg actcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga
     9361 gagagaatat aaaaagccag attattaatc cggctttttt attatttaat tcctgttata
     9421 aaaaaaggat caattttgaa ctctctccca aagttgatcc cttaacgatc taaaacaatt
     9481 catccagtaa aatataatat tttattttct cccaatcagg cttgatcccc agtaagtcaa
     9541 aaaatagctc gacatactgt tcttccccga tatcctccct gatcgaccgg acgcagaagg
     9601 caatgtcata ccacttgtcc gccctgccgc ttctcccaag atcaataaag ccacttactt
     9661 tgccatcttt cacaaagatg ttgctgtctc ccaggtcgcc gtgggaaaag acaagttcct
     9721 cttcgggctt ttccgtcttt aaaaaatcat acagctcgcg cggatcttta aatggagtgt
     9781 cttcttccca gttttcgcaa tccacatcgg ccagatcgtt attcagtaag taatccaatt
     9841 cggctaagcg gctgtctaag ctattcgtat agggacaatc cgatatgtcg atggagtgaa
     9901 agagcctgat gcactccgca tacagctcga taatcttttc agggctttgt tcatcttcat
     9961 actcttccga gcaaaggacg ccatcggcct cactcatgag cagattgctc cagccatcat
    10021 gccgttcaaa gtgcaggacc tttggaacag gcagctttcc ttccagccat agcatcatgt
    10081 ccttttcccg ttccacatca taggtggtcc ctttataccg gctgtccgtc atttttaaat
    10141 ataggttttc attttctccc accagcttat ataccttagc aggagacatt ccttccgtat
    10201 cttttacgca gcggtatttt tcgatcagtt ttttcaattc cggtgatatt ctcattttag
    10261 ccatttatta tttccttcct cttttctaca gtatttaaag ataccccaag aagctaatta
    10321 taacaagacg aactccaatt cactgttcct tgcattctaa aaccttaaat accagaaaac
    10381 agctttttca aagttgtttt caaagttggc gtataacata gtatcgacgg agccgatttt
    10441 gaaaccacaa ttatgataga atttacaagc tataaggtta ttgtcctggg tttcaagcat
    10501 tagtccatgc aagtttttat gctttgccca ttctatagat atattgataa gaagactgga
    10561 atgcccgctc cgccgggatt attcgaaagc gcatcgtcca agcgagaccg tctgcattcg
    10621 gaaatctcgg ccggttcgcg ccgaggaagc ccatctggct gcatcccaaa tcgacacaca
    10681 acgcacggag gccccggcga tcgccggggc ctccgaatca tgcgcatcgt ggaagcgaag
    10741 ctccgcaaaa aaccccgctt cagcggggtt ttttcgcgtc gcacctcctt gtcgtgtatt
    10801 acaggtacta ctataacaca tcatctagcc cgcccgcgat acgtcctctg cggtgcagcg
    10861 tggtcgcggt tccacgcatc cgccgcccgc tgcgcatcgc gcagcacgtc attaaggctc
    10921 tgttgccttg gctgttccct gcgcatcaga tattcgagcg ggcgcgatat tcgacttcta
    10981 agctcttcca ctttgattcg cattcccgca actcctgctc tgatgcggct cgcgagtcca
    11041 tcgcacttaa caacgatgtt atcgaggatt gcgccgcgct cagcttttcc cgcagcttcc
    11101 cagccccgaa cctcggtagc gatgggttga agctctccaa tgtctcgcgc aactccgtcc
    11161 gctcgttgcc gaagacactc caatcggtct tgctcatctt cgatatcgag cttcacgtcc
    11221 tcgagctggc tgtcaagctc ggcaaggtca ccctccttgt cggcaatctc ggtaacaagc
    11281 tctgccttac gcgtttcggc atcattcagt aaagcggcgg cttcttccct agccgccgcg
    11341 acgattcgcg ccgcttcgtc cttcgcaggt tcaaccacgg ccttatcgac cgcacagcgt
    11401 accttgtcga tatccacgga cttgtccgtg tatacacgct gcgcccgcgt ctcctcgtca
    11461 agctcgattg cagggcggta gcccatgcgc ccctctaggt aatccccaag ctccttgtgc
    11521 atgctccggt agaagctgcg ggggcacatc tgcttgtagt tgaaccgtcc atctaggatg
    11581 ggcgtgaacg gcacgtgcat gtggtcgcgg gcgggcgtgc catccttcaa cacctcgtcc
    11641 ttgtggacgt acccgcccat catgttctcg atgccgaact tcttcgacag gtaccagtac
    11701 gtgagacgga agaacctgtc ctcatcgccc ttgcgcacat tgtcgggaag cgtcacaaca
    11761 acgtcagcca taacgaccgc atccttgcgc gtggcacgct tccctgcggc cttctgggcc
    11821 tcgttcacgc gctcgatgcg gctctccacc gtgccccagt tcggctcgcc atcgcgcggc
    11881 tccacgcgcc ccacaacgag cttgccgtcc ttgttcgtgt agtggccgac caccaagtcg
    11941 ttcttgatgc gctcggggtc gatgttgtcg cgctccaggc tgctgcggtc gcggcggtag
    12001 tgggcaagca tgtgcccgac cgatggagcc ttgtatttcg cgatgtgagc catggaaacc
    12061 tccgaaccgt acagacttaa tcgactaaag tctagcacgt tagaccttag ctacgctaag
    12121 gacgtggcag ggccagccct gcacccgctc gggcgttccc cccgagaccc cccagccctt
    12181 gcccgttcgc ggcttcgctc acggccctcg ggccatcggc gcgagcgccg aacggaggga
    12241 cttcgcccct ccgaaccacc cctcaatcta cctgcggttc ggcacacggc acacggcact
    12301 attagggggg ccgtcgtagt ggcccccctg tggtcatttg tcatttgtca tttgtcacat
    12361 tatttttcgg acacattatt tttcggacgt agttagcgaa aaataatgag ccgcccgatt
    12421 ctgcaggtct catccagtcg agacacgggc aaggaaaggg tcgatggagc agagcgtcca
    12481 tcgaccctta atcaagtctg gtctattcgg ttggccgtcg cgtcaattcc gcaatccgtg
    12541 aacgagtcgg cttagatctg cgcgagcgca gaccacgccg ccccgtagtc acgcattgct
    12601 gcattgaccc gactactctt tggcacctga ctgttccacg cacttgcgct tacgttcggc
    12661 gttcagatag ttcccgcgac aatagcgctc gagaatcgag ttgtacccca cgatgatttc
    12721 gcggtactgc tcgggaaact ccctcagcgc cgcagccgtg aaatcgttga tggtctcgaa
    12781 cctctcgcac atgatgaagc cgaccaactc gaagagcatg tcctgcttgt ccttagtcga
    12841 gagctgttcg agctgaccaa tgtcgaagtt gtttccctcg atgcgtgctt cgaccggata
    12901 cagctccttg cccttcttgc gacaggaagc cgtgtcgtga atcagatagt catacgcatg
    12961 cctgatgttg ttgattggct cggctgacga acagcacttc ttgccgtccg cgctcaatcg
    13021 gttcaagacg ttgatgattg ccttgcgtgt cgtgttgccg ccccagacaa caatgacgtg
    13081 cgcatgcgtc ttgcgctgct tgctcttctg gtcatggtct atgtcgtgaa ccgcataagc
    13141 gaaaggcact tgaagaatgt cgtctatctc gtcctgccag tcatcgcgca tgttctctgg
    13201 gtagcagatg ccctcccaga gcttcacctt cgtctcgcca gcgtttgccg ccatgctata
    13261 atctccttgt ctcgaatgcc cctgctgccg cca
//
