LOCUS       sequence-463822-        5844 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To Express a Rat Arc-DsRed fusion protein from a CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5844)
  AUTHORS   de Solis CA, Holehonnur R, Banerjee A, Luong JA, Lella SK, Ho A, 
            Pahlavan B, Ploski JE
  TITLE     Viral delivery of shRNA to amygdala neurons leads to neurotoxicity 
            and deficits in Pavlovian fear conditioning.
  JOURNAL   Neurobiol Learn Mem. 2015 Oct;124:34-47. doi: 
            10.1016/j.nlm.2015.07.005. Epub 2015 Jul 13.
  PUBMED    26182988
REFERENCE   2  (bases 1 to 5844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neurobiol 
            Learn Mem."; date: "2015-10"; volume: "124:34-47. doi"; pages: " 
            10.1016/j.nlm.2015.07.005. Epub 2015 Jul 13"
COMMENT     Sequence Label: pDsRed1-CMV-Rat Arc-DsRed1-pA
FEATURES             Location/Qualifiers
     source          1..5844
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        764..1067
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1068..1271
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      2528..2537
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2534..2536,2537..2539,2540..3214)
                     /codon_start=1
                     /product="wild-type DsRed"
                     /label=DsRed1
                     /note="mammalian codon-optimized"
                     /translation="MVRSSKNVIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTV
                     KLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     KALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRH
                     HLFL"
     primer_bind     complement(2717..2737)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     2935..2954
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     3129..3152
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    3335..3456
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3372..3391)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3426..3445
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3463..3918)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3600..3621)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3812..3831
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3945..4049
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4051..4408
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4051..4242
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4074..4094)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4259..4394
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4443..5237
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4443..5237
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4497..4516)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5107..5126
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5425..5444)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5469..5516
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccatg cattagttat taatagtaat
      721 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
      781 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
      841 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
      901 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
      961 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     1021 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     1081 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     1141 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     1201 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     1261 taagcagagc tggtttagtg aaccgtcaga tccgctagcg ctaccggact cagatctcga
     1321 gcagatggag ctggaccata tgacgaccgg cggcctccac gcctaccctg ccccgcgggg
     1381 tgggccggcc gccaaaccca atgtgatcct gcagattggt aagtgccgag ctgagatgct
     1441 ggagcacgta cggaggaccc accggcatct gttgaccgaa gtgtccaagc aggtggagcg
     1501 agagctgaaa gggttgcaca ggtcggtggg caagctggag aacaacttgg acggctatgt
     1561 gcccacgggc gactcacagc gctggaagaa gtccatcaag gcctgtctct gccgctgcca
     1621 ggagaccatc gccaacctgg agcgctgggt caagcgtgag atgcacgtgt ggagggaggt
     1681 cttctaccgt ctggagaggt gggccgaccg cctggagtcc atgggcggca agtacccagt
     1741 gggcagcgag ccggcccgcc acactgtctc tgtaggtgtg gggggtccag agccctactg
     1801 ccaggaagct gatggctacg actacactgt tagcccctat gccatcaccc cgccacctgc
     1861 cgcaggagag ctgcctgagc aggagtcagt tggggctcag caataccagt cttgggtgcc
     1921 aggtgaggat gggcaaccaa gcccaggtgt ggatacccag atctttgagg acccacggga
     1981 gttcctgagc cacctggaag agtacctgcg gcaggtgggt ggctctgaag aatattggct
     2041 gtcccagatc cagaaccaca tgaatgggcc agccaagaag tggtgggagt tcaaacaggg
     2101 ctcggtgaag aactgggtgg agttcaagaa ggagtttctg cagtacagtg agggtacgct
     2161 ctcccgcgaa gccattcagc gggagctgga cctgccacag aagcagggtg agccacttga
     2221 ccagttcctc tggcgtaagc gggacctgta ccagacactg tatgtggacg ctgaggagga
     2281 ggagatcatt cagtatgtgg tgggcaccct gcagcccaag ttcaagcgct ttctgcgcca
     2341 cccactcccc aagaccctgg agcagctcat ccagaggggc atggaagttc aggacggcct
     2401 ggagcaggca gctgagcctt ctgtcacccc tctgcccaca gaggatgaga ctgaggcact
     2461 cacgcctgct cttaccagcg agtcagtagc cagtgacagg acccagcctg aacgggatcc
     2521 accggtcgcc accatggtgc gctcctccaa gaacgtcatc aaggagttca tgcgcttcaa
     2581 ggtgcgcatg gagggcaccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg
     2641 ccgcccctac gagggccaca acaccgtgaa gctgaaggtg accaagggcg gccccctgcc
     2701 cttcgcctgg gacatcctgt ccccccagtt ccagtacggc tccaaggtgt acgtgaagca
     2761 ccccgccgac atccccgact acaagaagct gtccttcccc gagggcttca agtgggagcg
     2821 cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga
     2881 cggctgcttc atctacaagg tgaagttcat cggcgtgaac ttcccctccg acggccccgt
     2941 aatgcagaag aagaccatgg gctgggaggc ctccaccgag cgcctgtacc cccgcgacgg
     3001 cgtgctgaag ggcgagatcc acaaggccct gaagctgaag gacggcggcc actacctggt
     3061 ggagttcaag tccatctaca tggccaagaa gcccgtgcag ctgcccggct actactacgt
     3121 ggactccaag ctggacatca cctcccacaa cgaggactac accatcgtgg agcagtacga
     3181 gcgcaccgag ggccgccacc acctgttcct gtagcggccg cgactctaga tcataatcag
     3241 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
     3301 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
     3361 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     3421 tagttgtggt ttgtccaaac tcatcaatgt atcttaaggc gtaaattgta agcgttaata
     3481 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
     3541 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
     3601 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
     3661 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
     3721 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
     3781 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
     3841 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
     3901 cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     3961 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     4021 tgcttcaata atattgaaaa aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt
     4081 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     4141 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     4201 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     4261 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     4321 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     4381 cttttttgga ggcctaggct tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc
     4441 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
     4501 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
     4561 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
     4621 tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
     4681 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
     4741 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
     4801 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
     4861 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
     4921 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg
     4981 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
     5041 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
     5101 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
     5161 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
     5221 ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa
     5281 cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
     5341 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
     5401 cgcccaccct agggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg
     5461 cgctatgacg gcaataaaaa gacagaataa aacgcacggt gttgggtcgt ttgttcataa
     5521 acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg
     5581 ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc
     5641 agggctcgca gccaacgtcg gggcggcagg ccctgccata gcctcaggtt actcatatat
     5701 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     5761 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     5821 cgtagaaaag atcaaaggat cttc
//
