LOCUS       sequence-46384-e         789 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Lian X, Hsiao C, Wilson G, Zhu K, Hazeltine LB, Azarin SM, Raval KK,
            Zhang J, Kamp TJ, Palecek SP
  TITLE     Robust cardiomyocyte differentiation from human pluripotent stem 
            cells via temporal modulation of canonical Wnt signaling.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 May 29.
  PUBMED    22645348
REFERENCE   2  (bases 1 to 789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2012 May 29."
COMMENT     Sequence Label: pXL004-ishRNA-scramble
FEATURES             Location/Qualifiers
     source          1..789
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    18..36
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             complement(369..413)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gatagagact tataagttcc ctatcagtga tagagaaaaa gctacactat cgagcaattt
       61 tggatccaaa attgctcgat agtgtagctt ttttttacta gtgttaaccg ataccgtcga
      121 gatccgttca ctaatcgaat ggatctgtct ctgtctctct ctccaccttc ttcttctatt
      181 ccttcgggcc tgtcgggtcc cctcggggtt gggaggtggg tctgaaacga taatggtgaa
      241 tatccctgcc taactctatt cactatagaa agtacagcaa aaactattct taaacctacc
      301 aagcctccta ctatcattat gaataatttt atataccaca gccaatttgt tatgttaaac
      361 caattccaca aacttgccca tttatctaat tccaataatt cttgttcatt cttttcttgc
      421 tggttttgcg attcttcaat taaggagtgt attaagcttg tgtaattgtt aatttctctg
      481 tcccactcca tccaggtcgt gtgattccaa atctgttcca gagatttatt actccaacta
      541 gcattccaag gcacagcagt ggtgcaaatg agttttccag agcaacccca aatccccagg
      601 agctgttgat cctttaggta tctttccaca gccaggattc ttgcctggag ctgcttgatg
      661 ccccagactg tgagttgcaa cagatgctgt tgcgcctcaa tagccctcag caaattgttc
      721 tgctgctgca ctataccaga caataattgt ctggcctgta ccgtcagcgt cattgacgct
      781 gcgcccata
//
