LOCUS       sequence-46392-e         730 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A positive control receptor bait with bio-linker-His (rat 
            CD4d3+4-bio-linker-his) and prey (rat CD200 receptor) EXPRESs 
            plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Bushell KM, Sollner C, Schuster-Boeckler B, Bateman A, Wright GJ
  TITLE     Large-scale screening for novel low-affinity extracellular protein 
            interactions.
  JOURNAL   Genome Res. 2008 Apr;18(4):622-30. Epub 2008 Feb 22.
  PUBMED    18296487
REFERENCE   2  (bases 1 to 730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Res."; date: "2008-04"; volume: "18(4)"; pages: "622-30. Epub 2008 
            Feb 22"
COMMENT     Sequence Label: CD200RCD4d3+4-bio-linker-His
FEATURES             Location/Qualifiers
     source          1..730
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     501..520
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      567..576
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gtttagtgaa ccgtcagatc ctcactctct tccgcatcgc tgtctgcgag ggccagctgt
       61 tgggctcgcg gttgaggaca aactcttcgc ggtctttcca gtactcttgg atcggaaacc
      121 cgtcggcctc cgaacggtac tccgccaccg agggacctga gcgagtccgc atcgaccgga
      181 tcggaaaacc tctcgagaaa ggcgtctaac cagtcacagt cgcaaggtag gctgagcacc
      241 gtggcgggcg gcagcgggtg gcggtcgggg ttgtttctgg cggaggtgct gctgatgatg
      301 taattaaagt aggcggtctt gagacggcgg atggtcgagg tgaggtgtgg caggcttgag
      361 atccagctgt tggggtgagt actccctctc aaaagcgggc attacttctg cgctaagatt
      421 gtcagtttcc aaaaacgagg aggatttgat attcacctgg cccgatctgg ccatacactt
      481 gagtgacaat gacatccact ttgcctttct ctccacaggt gtccactccc aggtccaagt
      541 ttaaactgcg gccgctccgg ctagccgcca ccatggtctg cttttggaga acttctcacg
      601 tagcagtact cttgatctgg ggggtcttcg cggctgagtc aagttgtcct gataagaatc
      661 aaacaatgca gaacaattca tcaactatga cagaagttaa cactacagtg tttgtacaga
      721 tgggtaaaaa
//
