LOCUS       sequence-46394-e         763 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Xie J, Ameres SL, Friedline R, Hung JH, Zhang Y, Xie Q, Zhong L, Su 
            Q, He R, Li M, Li H, Mu X, Zhang H, Broderick JA, Kim JK, Weng Z, 
            Flotte TR, Zamore PD, Gao G
  TITLE     Long-term, efficient inhibition of microRNA function in mice using 
            rAAV vectors.
  JOURNAL   Nat Methods. 2012 Mar 4;9(4):403-9. doi: 10.1038/nmeth.1903.
  PUBMED    22388288
REFERENCE   2  (bases 1 to 763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-03-4- 4"; volume: "9"; 
            issue: "4"; pages: "403-9"
COMMENT     Sequence Label: pAAVscCBPITuDlet-7Gpluc7xlet7BT
FEATURES             Location/Qualifiers
     source          1..763
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(149..706)
                     /codon_start=1
                     /product="secreted Gaussia luciferase"
                     /label=hGLuc
                     /note="human codon-optimized"
                     /translation="MGVKVLFALICIAVAEAKPTENNEDFNIVAVASNFATTDLDADRG
                     KLPGKKLPLEVLKEMEANARKAGCTRGCLICLSHIKCTPKMKKFIPGRCHTYEGDKESA
                     QGGIGEAIVDIPEIPGFKDLEPMEQFIAQVDLCVDCTTGCLKGLANVQCSDLLKKWLPQ
                     RCATFASKIQGQVDKIKGAGGD"
ORIGIN
        1 ggtttgaggt aggttttgta cagtttgagg taggttttgt atagttagag gtaggttttg
       61 catagtttga ggtaggtttt gtatggtttg aggtaggttt tgtgtggttt gaggtaggtt
      121 ttgtatagtt tctaggatca gcgtcgagct agtcaccacc ggcccccttg atcttgtcca
      181 cctggccctg gatcttgctg gcaaaggtcg cacagcgttg cggcagccac ttcttgagca
      241 ggtcagaaca ctgcacgttg gcaagccctt tgaggcagcc agttgtgcag tccacacaca
      301 gatcgacctg tgcgatgaac tgctccatgg gctccaagtc cttgaaccca ggaatctcag
      361 gaatgtcgac gatcgcctcg cctatgccgc cctgtgcgga ctctttgtcg ccttcgtagg
      421 tgtggcagcg tcctgggatg aacttcttca tcttgggcgt gcacttgatg tgggacaggc
      481 agatcagaca gcccctggtg cagccagctt tccgggcatt ggcttccatc tctttgagca
      541 cctccagcgg cagcttcttg ccgggcaact tcccgcggtc agcatcgaga tccgtggtcg
      601 cgaagttgct ggccacggcc acgatgttga agtcttcgtt gttctcggtg ggcttggcct
      661 cggccacagc gatgcagatc agggcaaaca gaactttgac tcccatgcta gatggtggcg
      721 accggtggat cggccgcggg tacaattccg cagcttttag agc
//
