LOCUS       sequence-46402-e         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Xie J, Ameres SL, Friedline R, Hung JH, Zhang Y, Xie Q, Zhong L, Su 
            Q, He R, Li M, Li H, Mu X, Zhang H, Broderick JA, Kim JK, Weng Z, 
            Flotte TR, Zamore PD, Gao G
  TITLE     Long-term, efficient inhibition of microRNA function in mice using 
            rAAV vectors.
  JOURNAL   Nat Methods. 2012 Mar 4;9(4):403-9. doi: 10.1038/nmeth.1903.
  PUBMED    22388288
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-03-4- 4"; volume: "9"; 
            issue: "4"; pages: "403-9"
COMMENT     Sequence Label: pRNA-U6-let7 sponge
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(175..423)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(233..252)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(403..423)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(553..572)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(554..572)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     744..763
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 agggccctga ggtaggtttt gtatggtttg aggtaggttt tgtacagttt gaggtaggtt
       61 ttgtatagtt agaggtaggt tttgcatagt ttgaggtagg ttttgtatgg tttgaggtag
      121 gttttgtgtg gtttgaggta ggttttgtat agtttctaga attacacggc gatggatccc
      181 gcgtcctttc cacaagatat ataaacccaa gaaatcgaaa tactttcaag ttacggtaag
      241 catatgatag tccattttaa aacataattt taaaactgca aactacccaa gaaattatta
      301 ctttctacgt cacgtatttt gtactaatat ctttgtgttt acagtcaaat taattctaat
      361 tatctctcta acagccttgt atcgtatatg caaatatgaa ggaatcatgg gaaataggcc
      421 ctcttcctgc ccgaccttgg cgcgcgctcg gcgcgcggtc acgctccgtc acgtggtgcg
      481 ttttgcctgc gcgtctttcc actggggaat tcatgcttct cctcccttta gtgagggtaa
      541 ttctctctct ctccctatag tgagtcgtat taaacgcgta tatctggccc gtacatcgcg
      601 aagcagcgca aaacgcctaa ccctaagcag attcttcatg caattgtcgg tcaagccttg
      661 ccttgttgta gcttaaattt tgctcgcgca ctactcagcg acctccaaca cacaagcagg
      721 gagcagatac tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatag
      781 gggatcggga gatctcccga tccgtcgacg tcaggtggca cttttcggg
//
