LOCUS       sequence-46404-e         596 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 596)
  AUTHORS   de Marco A, Deuerling E, Mogk A, Tomoyasu T, Bukau B
  TITLE     Chaperone-based procedure to increase yields of soluble recombinant 
            proteins produced in E. coli.
  JOURNAL   BMC Biotechnol. 2007 Jun 12;7:32. doi: 10.1186/1472-6750-7-32.
  PUBMED    17565681
REFERENCE   2  (bases 1 to 596)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2007-06-12"; volume: "7:32. doi"; pages: " 
            10.1186/1472-6750-7-32"
COMMENT     Sequence Label: pBB550
FEATURES             Location/Qualifiers
     source          1..596
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    111..127
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    144..160
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             179..190
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 catgccatac cgcgaaaggt tttgcaccat tcgatggtgt caacgtaaat gcatgccgct
       61 tcgccttcgc gcgcgaattg tcgagaaaat ttatcaaaaa gagtgttgac ttgtgagcgg
      121 ataacaatga tacttagatt caattgtgag cggataacaa tttcacacag aattcattaa
      181 agaggagaaa ggatccatgg gtaaaataat tggtatcgac ctgggtacta ccaactcttg
      241 tgtagcgatt atggatggca ccactcctcg cgtgctggag aacgccgaag gcgatcgcac
      301 cacgccttct atcattgcct atacccagga tggtgaaact ctagttggtc agccggctaa
      361 acgtcaggca gtgacgaacc cgcaaaacac tctgtttgcg attaaacgcc tgattggtcg
      421 ccgcttccag gacgaagaag tacagcgtga tgtttccatc atgccgttca aaattattgc
      481 tgctgataac ggcgacgcat gggtcgaagt taaaggccag aaaatggcac cgccgcagat
      541 ttctgctgaa gtgctgaaaa aaaatgaaga aaaccgctga agattacctg ggtgaa
//
