LOCUS       sequence-464050-        9158 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Visualization of cAMP dynamics using YR-ICUE-mVenus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9158)
  AUTHORS   Lee J, Lai S, Yang S, Zhao S, Blanco FA, Lyons AC, Merino-Urteaga R,
            Ahrens JF, Nguyen NA, Liu H, Liu Z, Lambert GG, Shaner NC, Chen L, 
            Tolias KF, Zhang J, Ha T, St-Pierre F
  TITLE     Bright and photostable yellow fluorescent proteins for extended 
            imaging.
  JOURNAL   Nat Commun. 2025 Apr 4;16(1):3241. doi: 10.1038/s41467-025-58223-5.
  PUBMED    40185748
REFERENCE   2  (bases 1 to 9158)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2025-04-4"; volume: "16(1):3241. doi"; pages: " 
            10.1038/s41467-025-58223-5"
COMMENT     Sequence Label: pCMV-YR-ICUE-mVenus
FEATURES             Location/Qualifiers
     source          1..9158
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     12..31
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(250..354)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(429..448)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        620..999
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1000..1203
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1154..1174
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1248..1267
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1248..1266
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1286..1295
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1307..1324
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1328..1360
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(1364..1372,1373..1387)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             1394..2101
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1539..1557)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1807..1826
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             join(4313..4315,4316..4318,4319..5032)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4358..4379)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4619..4638)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4966..4987
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        complement(5096..5114)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5097..5114)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5134..5151)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5140..5364
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5410..5838
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5497..5516)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5707..5728
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5847..5867)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5852..6181
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6032..6167
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6094..6113
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6248..7042
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6302..6321)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6912..6931
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7216..7337
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7253..7272)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7307..7326
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7386..7402)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7386..7402)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7399..7421)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7410..7426
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7434..7440,7441..7458,7459..7464))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7479..7500
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7617..7634)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7788..8376)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7868..7887)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8547..9158,1..180,181..249))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
       61 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
      121 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
      181 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
      241 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
      301 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
      361 tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtgc
      421 actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt
      481 gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg
      541 accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta
      601 cgggccagat atacgcgttg acattgatta ttgactagtt attaatagta atcaattacg
      661 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
      721 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
      781 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
      841 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
      901 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
      961 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
     1021 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
     1081 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
     1141 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga
     1201 gctctctggc taactagaga acccactgct tactggctta tcgaaattaa tacgactcac
     1261 tatagggaga cccaagcttg cggccgccac catggtgcgg ggttctcatc atcatcatca
     1321 tcatggtatg gctagcatga ctggtggaca gcaaatgggt cgggatctgt acgacgatga
     1381 cgataaggat cccatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt
     1441 catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg
     1501 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg
     1561 tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc
     1621 ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt
     1681 caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc
     1741 ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc
     1801 cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta
     1861 ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg
     1921 ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg
     1981 cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt
     2041 ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa
     2101 gagatctccc gtgggaactc atgagatgga ggaggagttg gccgaagctg tggccctgct
     2161 ctcccagcgg gggcctgacg ccctgctcac tgtggcactt cgaaagcccc caggtcagcg
     2221 cacggatgaa gagctggacc tcatctttga ggagctgctg cacatcaagg ctgtggccca
     2281 cctctccaac tcggtgaagc gagaattagc ggctgttctg ctctttgaac cacacagcaa
     2341 ggcagggacc gtgttgttca gccaggggga caagggcact tcgtggtaca ttatctggaa
     2401 gggatctgtc aacgtggtga cccatggcaa ggggctggtg accaccctgc atgagggaga
     2461 tgattttgga cagctggctc tggtgaatga tgcaccccgg gcagccacca tcatcctgcg
     2521 agaagacaac tgtcatttcc tgcgtgtgga caagcaggac ttcaaccgta tcatcaagga
     2581 tgtggaggca aagaccatgc ggctggaaga acatggcaaa gtggtgctgg tgctggagag
     2641 agcctctcag ggcgccggcc cttcccgacc cccaacccca ggcaggaacc ggtatacagt
     2701 gatgtctggc accccagaga agatcctaga gcttctgttg gaggccatgg gaccagattc
     2761 cagtgctcat gaccaaacag agacattcct cagcgacttc ctcctgaccc acagggtctt
     2821 catgcccagc gcccaactct gcgctgccct tctgcaccac ttccatgtgg agcctgcggg
     2881 tggcagcgag caggagcgca gcacctacgt ctgcaacaag aggcagcaga tcttgcggct
     2941 ggtcagccag tgggtggccc tgtatggctc catgctccac actgaccctg tggccaccag
     3001 cttcctccag aaactctcag acctggtggg cagggacacc cgactcagca acctgctgag
     3061 ggagcagtgg ccagagaggc ggcgatgcca caggttggag aatggctgtg ggaatgcatc
     3121 tcctcagatg aaggcccgga acttgcctgt ttggctcccc aaccaggacg agccccttcc
     3181 tggcagcagc tgtgccatcc aagttgggga taaagtcccc tatgacatct gccggccaga
     3241 ccactcagtg ttgaccctgc agctgcctgt gacagcctcc gtgagagagg tgatggcagc
     3301 gttggcccag gaggatggct ggaccaaggg gcaggtgctg gtgaaggtca attctgcagg
     3361 tgatgccatt ggcctgcagc cagatgcccg tggtgtggcc acatctctgg ggctcaatga
     3421 gcgtctcttt gttgtcaacc cacaggaagc gcatgagctg atcccacacc ctgaccagct
     3481 ggggcccact gtgggctctg ctgaggggct ggacctggtg agtgccaagg acctggcagg
     3541 ccagctgacg gaccacgact ggagcctctt caacagtatc caccaggtgg agctgatcca
     3601 ctatgtgctg ggcccccagc atctgcggga tgtcaccacc gccaacctgg agcgcttcat
     3661 gcgccgcttc aatgagctgc agtactgggt ggccaccgag ctgtgtctct gccccgtgcc
     3721 cggcccccgg gcccagctgc tcaggaagtt cattaagctg gcggcccacc tcaaggagca
     3781 gaagaatctc aattccttct ttgccgtcat gtttggcctc agcaactcgg ccatcagcca
     3841 cctagcccac acctgggagc ggctgcccca caaagtccgg aagctgtact ccgccctcga
     3901 gaggctgctg gacccctcat ggaaccaccg ggtataccga ctggccctcg ccaagctctc
     3961 ccctcctgtc atccccttca tgccccttct tctcaaagac atgaccttca ttcatgaggg
     4021 aaaccacaca ctagtggaga atctcatcaa ctttgagaag atgagaatga tggccagagt
     4081 cgcgcggatg ctgcaccact gccgaagcca caaccctgtg cctctctcac cactcagaag
     4141 ccgagtttcc cacctccacg aggacagcca ggtggcgagg atttccacat gctcggagca
     4201 gtccctgagc acccggagtc cagccagcac ctgggcttat gtccagcagc tgaaggtcat
     4261 tgacaaccag cgggaactct cccgcctctc ccgagagctg gagccaggta ccatggtgag
     4321 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
     4381 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
     4441 gaccctgaag ctgatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
     4501 caccctgggc tacggcctgc agtgcttcgc ccgctacccc gaccacatga agcagcacga
     4561 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
     4621 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
     4681 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
     4741 gtacaactac aacagccaca acgtctatat caccgccgac aagcagaaga acggcatcaa
     4801 ggccaacttc aagatccgcc acaacatcga ggacggcggc gtgcagctcg ccgaccacta
     4861 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     4921 ctaccagtcc aagctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     4981 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt aagaattcaa
     5041 gaagaagaag aagagcaaga ccaagtgcgt gatcatgtaa tctagagggc cctattctat
     5101 agtgtcacct aaatgctaga gctcgctgat cagcctcgac tgtgccttct agttgccagc
     5161 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     5221 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     5281 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     5341 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg
     5401 ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     5461 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     5521 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     5581 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     5641 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     5701 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     5761 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     5821 aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     5881 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     5941 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     6001 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     6061 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     6121 tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     6181 aaagctcccg ggagcttgta tatccatttt cggatctgat caagagacag gatgaggatc
     6241 gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
     6301 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
     6361 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
     6421 tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
     6481 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
     6541 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
     6601 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
     6661 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
     6721 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat
     6781 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt
     6841 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
     6901 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
     6961 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
     7021 ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg
     7081 cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
     7141 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
     7201 ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     7261 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     7321 ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa
     7381 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     7441 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     7501 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     7561 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     7621 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     7681 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     7741 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     7801 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     7861 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     7921 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     7981 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     8041 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     8101 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     8161 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     8221 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     8281 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     8341 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     8401 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     8461 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     8521 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     8581 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     8641 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     8701 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     8761 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     8821 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     8881 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     8941 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     9001 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     9061 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     9121 gaatagtgta tgcggcgacc gagttgctct tgcccggc
//
