LOCUS       sequence-46406-e         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Xie J, Ameres SL, Friedline R, Hung JH, Zhang Y, Xie Q, Zhong L, Su 
            Q, He R, Li M, Li H, Mu X, Zhang H, Broderick JA, Kim JK, Weng Z, 
            Flotte TR, Zamore PD, Gao G
  TITLE     Long-term, efficient inhibition of microRNA function in mice using 
            rAAV vectors.
  JOURNAL   Nat Methods. 2012 Mar 4;9(4):403-9. doi: 10.1038/nmeth.1903.
  PUBMED    22388288
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-03-4- 4"; volume: "9"; 
            issue: "4"; pages: "403-9"
COMMENT     Sequence Label: pRNA-U6-TuDmiR122 seedonly
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(124..372)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(182..201)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(352..372)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(502..521)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(503..521)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     693..712
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacggcgcta ggatcatcag tagtggagtg tctattatta ttataagatg agtattctgt
       61 gaccagaata cagtagtgga gtgtctatta ttattataag atgagatgat cctagcgccg
      121 tcggatcccg cgtcctttcc acaagatata taaacccaag aaatcgaaat actttcaagt
      181 tacggtaagc atatgatagt ccattttaaa acataatttt aaaactgcaa actacccaag
      241 aaattattac tttctacgtc acgtattttg tactaatatc tttgtgttta cagtcaaatt
      301 aattctaatt atctctctaa cagccttgta tcgtatatgc aaatatgaag gaatcatggg
      361 aaataggccc tcttcctgcc cgaccttggc gcgcgctcgg cgcgcggtca cgctccgtca
      421 cgtggtgcgt tttgcctgcg cgtctttcca ctggggaatt catgcttctc ctccctttag
      481 tgagggtaat tctctctctc tccctatagt gagtcgtatt aaacgcgtat atctggcccg
      541 tacatcgcga agcagcgcaa aacgcctaac cctaagcaga ttcttcatgc aattgtcggt
      601 caagccttgc cttgttgtag cttaaatttt gctcgcgcac tactcagcga cctccaacac
      661 acaagcaggg agcagatact ggcttaacta tgcggcatca gagcagattg tactgagagt
      721 gcaccatagg ggatcgggag atctcccgat ccgtcgacgt caggtggcac ttttcgggga
      781 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
      841 atgagacaat aaccctgata aatgcttcaa taata
//
