LOCUS       sequence-46407-e         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Xie J, Ameres SL, Friedline R, Hung JH, Zhang Y, Xie Q, Zhong L, Su 
            Q, He R, Li M, Li H, Mu X, Zhang H, Broderick JA, Kim JK, Weng Z, 
            Flotte TR, Zamore PD, Gao G
  TITLE     Long-term, efficient inhibition of microRNA function in mice using 
            rAAV vectors.
  JOURNAL   Nat Methods. 2012 Mar 4;9(4):403-9. doi: 10.1038/nmeth.1903.
  PUBMED    22388288
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-03-4- 4"; volume: "9"; 
            issue: "4"; pages: "403-9"
COMMENT     Sequence Label: pRNA-U6-TuDmiR122 seedsupplemental
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(113..361)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(171..190)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(341..361)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(491..510)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(492..510)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     682..701
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gatcatcagt agtggagtgt ctgaatggat aataggatga gtattctgtg accagaatac
       61 agtagtggag tgtctgaatg gataatagga tgagatgatc ctagcgccgt cggatcccgc
      121 gtcctttcca caagatatat aaacccaaga aatcgaaata ctttcaagtt acggtaagca
      181 tatgatagtc cattttaaaa cataatttta aaactgcaaa ctacccaaga aattattact
      241 ttctacgtca cgtattttgt actaatatct ttgtgtttac agtcaaatta attctaatta
      301 tctctctaac agccttgtat cgtatatgca aatatgaagg aatcatggga aataggccct
      361 cttcctgccc gaccttggcg cgcgctcggc gcgcggtcac gctccgtcac gtggtgcgtt
      421 ttgcctgcgc gtctttccac tggggaattc atgcttctcc tccctttagt gagggtaatt
      481 ctctctctct ccctatagtg agtcgtatta aacgcgtata tctggcccgt acatcgcgaa
      541 gcagcgcaaa acgcctaacc ctaagcagat tcttcatgca attgtcggtc aagccttgcc
      601 ttgttgtagc ttaaattttg ctcgcgcact actcagcgac ctccaacaca caagcaggga
      661 gcagatactg gcttaactat gcggcatcag agcagattgt actgagagtg caccataggg
      721 gatcgggaga tctcccgatc cgtcgacgtc aggtggcact tttcggggaa atgtgcgcgg
      781 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagaca
//
