LOCUS       sequence-46408-e         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Xie J, Ameres SL, Friedline R, Hung JH, Zhang Y, Xie Q, Zhong L, Su 
            Q, He R, Li M, Li H, Mu X, Zhang H, Broderick JA, Kim JK, Weng Z, 
            Flotte TR, Zamore PD, Gao G
  TITLE     Long-term, efficient inhibition of microRNA function in mice using 
            rAAV vectors.
  JOURNAL   Nat Methods. 2012 Mar 4;9(4):403-9. doi: 10.1038/nmeth.1903.
  PUBMED    22388288
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-03-4- 4"; volume: "9"; 
            issue: "4"; pages: "403-9"
COMMENT     Sequence Label: pRNA-U6-TuDmiR122
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(122..370)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(180..199)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(350..370)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(500..519)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(501..519)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     691..710
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacggcgcta ggatcatctt gtggagtgtg aagatcaatg gtgtttggtt gtattctgtg
       61 accagaatac ttgtggagtg tgaagatcaa tggtgtttgg ttgatgatcc tagcgccgtc
      121 ggatcccgcg tcctttccac aagatatata aacccaagaa atcgaaatac tttcaagtta
      181 cggtaagcat atgatagtcc attttaaaac ataattttaa aactgcaaac tacccaagaa
      241 attattactt tctacgtcac gtattttgta ctaatatctt tgtgtttaca gtcaaattaa
      301 ttctaattat ctctctaaca gccttgtatc gtatatgcaa atatgaagga atcatgggaa
      361 ataggccctc ttcctgcccg accttggcgc gcgctcggcg cgcggtcacg ctccgtcacg
      421 tggtgcgttt tgcctgcgcg tctttccact ggggaattca tgcttctcct ccctttagtg
      481 agggtaattc tctctctctc cctatagtga gtcgtattaa acgcgtatat ctggcccgta
      541 catcgcgaag cagcgcaaaa cgcctaaccc taagcagatt cttcatgcaa ttgtcggtca
      601 agccttgcct tgttgtagct taaattttgc tcgcgcacta ctcagcgacc tccaacacac
      661 aagcagggag cagatactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      721 accatagggg atcgggagat ctcccgatcc gtcgacgtca ggtggcactt ttcggggaaa
      781 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaa
//
