LOCUS       sequence-46409-e         637 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 637)
  AUTHORS   Xie J, Ameres SL, Friedline R, Hung JH, Zhang Y, Xie Q, Zhong L, Su 
            Q, He R, Li M, Li H, Mu X, Zhang H, Broderick JA, Kim JK, Weng Z, 
            Flotte TR, Zamore PD, Gao G
  TITLE     Long-term, efficient inhibition of microRNA function in mice using 
            rAAV vectors.
  JOURNAL   Nat Methods. 2012 Mar 4;9(4):403-9. doi: 10.1038/nmeth.1903.
  PUBMED    22388288
REFERENCE   2  (bases 1 to 637)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-03-4- 4"; volume: "9"; 
            issue: "4"; pages: "403-9"
COMMENT     Sequence Label: pRNA-U6-ZipmiR122
FEATURES             Location/Qualifiers
     source          1..637
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(6..246)
                     /label=U6 promoter
                     /note="Truncated RNA polymerase III promoter for human U6 
                     snRNA used in pSIREN vectors such as 
                     pSIREN-RetroQ-ZsGreen1. Expected transcription start site 
                     is 8bp downstream of this feature."
     primer_bind     complement(56..75)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(226..246)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(376..395)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(377..395)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     567..586
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cccgcgtcct ttccacaaga tatataaacc caagaaatcg aaatactttc aagttacggt
       61 aagcatatga tagtccattt taaaacataa ttttaaaact gcaaactacc caagaaatta
      121 ttactttcta cgtcacgtat tttgtactaa tatctttgtg tttacagtca aattaattct
      181 aattatctct ctaacagcct tgtatcgtat atgcaaatat gaaggaatca tgggaaatag
      241 gccctcttcc tgcccgacct tggcgcgcgc tcggcgcgcg gtcacgctcc gtcacgtggt
      301 gcgttttgcc tgcgcgtctt tccactgggg aattcatgct tctcctccct ttagtgaggg
      361 taattctctc tctctcccta tagtgagtcg tattaaacgc gtatatctgg cccgtacatc
      421 gcgaagcagc gcaaaacgcc taaccctaag cagattcttc atgcaattgt cggtcaagcc
      481 ttgccttgtt gtagcttaaa ttttgctcgc gcactactca gcgacctcca acacacaagc
      541 agggagcaga tactggctta actatgcggc atcagagcag attgtactga gagtgcacca
      601 taggggatcg ggagatctcc cgatccgtcg acgtcag
//
