LOCUS       sequence-46419-e        1097 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral transfer plasmid for Hb9 GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Marchetto MC, Muotri AR, Mu Y, Smith AM, Cezar GG, Gage FH
  TITLE     Non-cell-autonomous effect of human SOD1 G37R astrocytes on motor 
            neurons derived from human embryonic stem cells.
  JOURNAL   Cell Stem Cell. 2008 Dec 4;3(6):649-57.
  PUBMED    19041781
REFERENCE   2  (bases 1 to 1097)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell."; date: "2008-12-4"; volume: "3(6)"; pages: "649-57"
COMMENT     Sequence Label: Lenti-Hb9-GFP
FEATURES             Location/Qualifiers
     source          1..1097
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(122..835,836..838,839..841))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(125..835,836..838,839..841))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(167..188)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     516..535
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     775..796
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     promoter        complement(873..911)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
ORIGIN
        1 gngantctgt ttgctacttg tgattgctcc atgtttttcc aggtttaaac tcgagacgtt
       61 aacgggccca ggcggccacc ggtggatcct ctagagttta aacggatccg aattggccgc
      121 tttacttgta cagctcgtcc atgccgagag tgatcccggc ggcggtcacg aactccagca
      181 ggaccatgtg atcgcgcttc tcgttggggt ctttgctcag ggcggactgg gtgctcaggt
      241 agtggttgtc gggcagcagc acggggccgt cgccgatggg ggtgttctgc tggtagtggt
      301 cggcgagctg cacgctgccg tcctcgatgt tgtggcggat cttgaagttc accttgatgc
      361 cgttcttctg cttgtcggcc atgatataga cgttgtggct gttgtagttg tactccagct
      421 tgtgccccag gatgttgccg tcctccttga agtcgatgcc cttcagctcg atgcggttca
      481 ccagggtgtc gccctcgaac ttcacctcgg cgcgggtctt gtagttgccg tcgtccttga
      541 agaagatggt gcgctcctgg acgtagcctt cgggcatggc ggacttgaag aagtcgtgct
      601 gcttcatgtg gtcggggtag cggctgaagc actgcacgcc gtaggtcagg gtggtcacga
      661 gggtgggcca gggcacgggc agcttgccgg tggtgcagat gaacttcagg gtcagcttgc
      721 cgtaggtggc atcgccctcg ccctcgccgg acacgctgaa cttgtggccg tttacgtcgc
      781 cgtccagctc gaccaggatg ggcaccaccc cggtgaacag ctcctcgccc ttgctcacca
      841 tggggcgcgc cggatctgcg gttcactaaa ccagctctgc ttatatagac ctcccaccgt
      901 acacgcctac cgatcttact gcaaattcat tgtataggag cttcatatca tgggcaggac
      961 aaagaacaga agcaagctcc aagcaggctg tgcagccctc tccccacctc cactaccact
     1021 gcagggcttg ataagtcatc cacagaaagg gtctgctttg ngtgataccc agcttccata
     1081 gctgggncga tgttacg
//
