LOCUS       sequence-464203-       11816 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses myristolated Akt2 in zebrafish lymphoid and mesenchymal 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11816)
  AUTHORS   Ali RH, Orellana EA, Lee SH, Chae YC, Chen Y, Clauwaert J, Kennedy 
            AL, Gutierrez AE, Papke DJ, Valenzuela M, Silverman B, Falzetta A, 
            Ficarro SB, Marto JA, Fletcher CDM, Perez-Atayde A, Alcindor T, 
            Shimamura A, Prensner JR, Gregory RI, Gutierrez A
  TITLE     A methyltransferase-independent role for METTL1 in tRNA 
            aminoacylation and oncogenic transformation.
  JOURNAL   Mol Cell. 2025 Mar 6;85(5):948-961.e11. doi: 
            10.1016/j.molcel.2025.01.003. Epub 2025 Jan 31.
  PUBMED    39892392
REFERENCE   2  (bases 1 to 11816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2025.01.003"; journalName: "Mol Cell"; date: 
            "2025-03-6- 6"; volume: "85"; issue: "5"; pages: "948-961.e11"
COMMENT     Sequence Label: rag2:myr-Akt2_pISceI
FEATURES             Location/Qualifiers
     source          1..11816
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      6587..6596
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6593..6634
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     CDS             6650..6676
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    8243..8377
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8267..8286)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8321..8340
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(8494..8510)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8494..8510)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(8564..8583)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8565..8583)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8593..8610)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8593..8609)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8602..8624)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(8750..9205)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8887..8908)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9099..9118
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        9232..9336
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9337..9405,9406..10197)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9555..9574)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10368..10956
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10857..10876
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11110..11127
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11244..11265
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11280..11285,11286..11303,11304..11310)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11318..11334
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11323..11345
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11342..11358
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11342..11358
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11377..11397
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11379..11397
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tcactgttct tcaggcactc gtggttacgg gtttttgttt tgcactcttg cacacacatc
       61 tctcggcctc caaaacaaac acacaactgc acgagggctc gaagccccca ccgaaaaaca
      121 aaaacattat gctaatgagg aaggcacagc tgttcccttc ttcactctct ctctcgttct
      181 ttcaacaaca gtcgcctaat ttctctcgtt cctttaaaga gccgccaagc agaaacagtt
      241 aatgcaaata taagcctgcg ggaaataagg agaattttta caaaatccga gcttcccctc
      301 ctgccaaaat gtgatgtgtg actcacgcat tcgatatttg gtgtaatgag agtgaatcat
      361 tctgctccac accgaacccg atgccacaga ccactcagat gcacaccgag gagactattt
      421 taacagcaca ttacatccag actgtcacag atgcattacc aagagtgtga tttcagtgtc
      481 gaatgcaggt cttaaaggaa aatctgttgc gattgcatgt gcgctaacta gacacgacca
      541 acgaatggga acaatctgtg aaatattata caatcacagg caattaaaag atgtttaaag
      601 ttcaatttag ttttaattaa tatgttatat aaaactaatc tgttttaaat gtatatttat
      661 gattttagct ttagctagcc tcttgaccag cagctgaagt gtccggcaag tacctaaaag
      721 ggagaccaca tgggaaagct aggttgctcc cggaagaggt gttagtaaac cagcaggtct
      781 acctgtggtc tgtgtgggtc ctaatgcccc agtataaacc agcaggtcaa cctgtggtct
      841 gtgtgggtcc taatgcccca gtataaacca gcaggtctac ctgtggtctg tgtgggtcct
      901 aatgccccag tataaaccag caggtcaacc tgtagtctgt gtgggtccta atgccccagt
      961 ataaaccagc aggtctacct gtggtctgtg tgggtcctaa tgccccagta taaaccaaca
     1021 ggtcaacctg tgatctgtgt gggtcctaat gccccagtat aaaccagcag gtcaacctgt
     1081 ggtctgtgtg ggtcctaatg ccccagtata aaccagcagg tcaacctgtg gtctgtgtgg
     1141 gtcctaatgc cccagtataa accagcaggt caacgtgtgg tctgtgtggg tcctaatgcc
     1201 ctggtataaa ccagcaggtc aacttgtggt ctgtgtgggt cctaatgccc cggtataaac
     1261 cagcatgtca acctgtggtc tgtgtgggtc ctaatgcccc ggtataaacc agcagatcaa
     1321 cctgtggtct gtgtgggtcc taatgcccca gtataaacca gcaggtcaac ctgtggtctg
     1381 tgtgggtcct aatgccccgg tacaaaccag catgtcaacc tgtggtctgt gtgggtccta
     1441 atgccccagt ataaaccagc aggtcaacct gtggtctgtg tgggtcctaa tgccccagta
     1501 taaaccagca ggtctacctg tggtctgtgt gggtcctaat gccccggtac aaaccagcat
     1561 gtcaacctgt ggtctgtgtg ggtcctaatg ccccagtata aaccagcagg tcaacctgtg
     1621 gtctgtgtgg gtcctaatgc cccagtataa accagcaggt ctacctgtgg tctgtgtggg
     1681 tcctaatgcc ccagtataaa ccagcaggtc aacctgtggt ctgtgtgggt cctaatgccc
     1741 cagtataaac cagcaggtct acctgtggtc tgtgtgggtc ctaatgcacc agtataaacc
     1801 agcaggtcaa cctgtggtct gtgtgggttc taatgcccca gtataaacca gcaggtcaac
     1861 ctgtggtctg tgtgggtcca aatgccccag tacaaaccag caggtcaacc tgtggtctgt
     1921 gtgggtccta atgccccagt ataaaccagc aggtcaacct gtggtctgtg tgggtcctaa
     1981 tgccccggta caaaccagca tgtcaacctg tggtctgtgt gggtcctaat gccccggtat
     2041 aaaccagcag atcaacctgt ggtctgtgtg ggtcctaatg ccccagtata aaccagcaga
     2101 tcaacctgtg gtctgtgtgg gtcctaaagt cccaggataa accagcatgt caacctgtgg
     2161 tctgtgtggg tcctaatgcc ccggtataaa ccagcagatc aacctgtggt ctgtgtgggt
     2221 cctaatgccc caggataaac cagcaggtca acctgtggtc tgtgtgggtc ctaatgcccc
     2281 agtataaacc agcaggtcaa cctgtggtct gtgtgggtcc taatgcccca gtatattgat
     2341 ggagactcta tactgctcgg tgagctccat ctttcggata agatgttaaa acaaggtccc
     2401 gactctctgt ggtggtaaaa aattcccagg atgtccttcc taccctggca tcctggacaa
     2461 atttgctcac tgtcctctgt ccatcatggc ctcctaacca tccccatatc ataattggct
     2521 ttatcactct agctggggcc gaaatcgcat acttccattt tatgaggtac gctaaaatca
     2581 gtatgcgagc caagtaatat gtccgaatac ttccggcgag attctagagt gtgcattcga
     2641 tgcacgctac gctattccat gatgccccgt gagagaattc ttgaatcgga gagaagcaac
     2701 gcaactcatg cgggtaggtt acgtgacgat gacatattgg cggatgtagt acgtccaagt
     2761 tccattcata ctgctcacat tcatatttta tagaatgttc ttttcccaca gccaagtagt
     2821 aagtttaaat tcaaatacag tacctactaa gtagtagtag acggtttcgg acgcagcccc
     2881 tgtctcctct ccaccaatat actggtgtgt agtgtgcggt ctggtgcaat atggctgcca
     2941 tcgcgtcatc caggtggatg ctgcacactg gtggttgatg aggagatttt gcccaaaaaa
     3001 gtgttttgag tttccagaaa agcgcgatat aaatgtaagg aattatcatt agcttattat
     3061 aatggaactt tctggataac ttgtgccaga gctaggggcc attaaaactt tgccctgggg
     3121 tacctcaggg agctgcaagg aagtgctata gggctaatga aatgtttggg ggaaaaaaaa
     3181 aaattttata agtaaaaata aaactaaaat aattaataat ttcattgttg aaaattatag
     3241 taaaattgat aaacaagata agcagcatta gtataaaaaa ctaaaaacaa acgtaattta
     3301 aaataattaa attgttttaa ataaattata caaaataatg tttacaacga cacggtggtg
     3361 cagtaggtag tgctgtcgcc agcaaagcaa gaaggtcgct ggttcgagcc tcagctggat
     3421 cacttgatgt ttctgtgtgg agcattcgcg tgcgtttaac cccagattaa taaagagact
     3481 aagccgaaaa gaaaataaat gtatgaaaat gtttacgtaa aatgtaaata aatgcatgat
     3541 taataaaata tatgaatttt aaagattgag gtagaattgt tttattattc acacatttgg
     3601 ttatttttaa tcagtgacaa cttgtgatgc tctttatatt gtttattacg ctactttgaa
     3661 tattcagtct caaattgcat gtaaatacga cttcaaagca gaattagttt aatttaattg
     3721 cctgtaaaca gtgttgtact ttgatggtga ttgtctgata ataatatgat ttcactattt
     3781 aaaatttgaa aaaaaaaaat ctgaaaaata ctcaccggcc actttattag gtacacctgt
     3841 ctaactgctt gttaacgtaa atttctaatc agccaatcac caggcagcaa ctcagtgcat
     3901 ttaggcatgt agacatggtc aagacgatct gctgcagttc aaagcgagca tcagaatgga
     3961 gaagcaaggt gatttaagtg actttaaacg tgacgtgttt gttggtgcca gacgggctgg
     4021 tctgagtatt tcagaaactg ctgatttact ggggttttca cacacaaaca tctctggggt
     4081 ttacagagaa tggtccaaaa aaagataata tccagtgagc ggcagttctg tgggggcaaa
     4141 tgtcttgttg atgccagagg tcagaggaga atggcctgac tggttccagc tgatagaaag
     4201 gcaacagtaa ttcaaataac cactcgttag aactgaggca tgcagaagag cacctttgaa
     4261 cacaacatgt cgaaccttga ggcgggtggg ctacagcagc tcaagaccac atcgggtgcc
     4321 actcctgtca gctaagaaca ggaaactgag gctacaattc gcacaggctc aacataagta
     4381 gacaatagaa gattgaacat tatgttgcct ggtcagatgg atacattcta ccttgtatca
     4441 acagttcagg ctggtgatgg tggtgtaatg gtgtggggga tatattcttg gcacactttg
     4501 ggcccattag taccaattaa ccatcgtgtc aacgccacag cctaactgag tattgttgct
     4561 gaccatgttc atttcttttt gaccacagtg tacccatctt ctgatggcta cttcctgcag
     4621 gataatgcac catgtcataa agcgtgaatg atctcagact ggtttcttga acatgacaat
     4681 gagttcactg tgctcgaatg acctccacag tcaccagatc tcaatccaat agagcgcctt
     4741 tggggtgtgg tagaatggaa gattcgcatc ctggatgtgc agccgacaaa tctgcatgat
     4801 gctagcatgt caatatggac caaaatctct gaggaatttt ccagtacctg gttgaaatgc
     4861 aaaaggaagg gaatctatgc taaggcaaaa agaggtccaa cccagtacta gtggggtgta
     4921 cataataaag tggccggtga gtgtatattg ttaaggatat ctagataatc caaataattt
     4981 acagcactat ttaaaaaaaa aactttatgt caccaagtct gcatttattt gatcacaatt
     5041 acagtcgaaa aagcaatatc gggatacatt gtttccattt aaaataacta tattatattt
     5101 taaatatttt tgacaatgtt atttctttaa tgccaaagct aaattgtatt caatctgaat
     5161 tttagtgtca catgatcctt cagaaaatat taacacattg ctaaaaatgt atattattat
     5221 caatttggat tttttttttt ttttgtatat taacctatgc tattggttac tatacaaaag
     5281 tattactgtg aatatatgca tatacagaac cgtatcaagc gcggggggtg gttgggttat
     5341 gtccccattt ggaaagacct ggaaacattc ataatgacta aaattgcacg gaaaaatata
     5401 atatctatga gtattgctga tcagcagcac aaaaccagaa ctccatatta ataaacagcc
     5461 aatgatgcag tgagtttgtg catgttttca ttcaaactcg tgtgattgtg ctcaaacacc
     5521 tgttgtgagc agcagttgca aacagtaaac ctcaagagga gccagtcaac cttcacaaac
     5581 actcaacctc tggctcactc actaaacaat tcgcgttaat atctaaaata aaaatttcca
     5641 gcaccaaaca gctctcagat ttcggagtac actgaccaag aggtaagaag taacgttaca
     5701 ttactaggac gactattgta agactagata tgtaatattt ggaagcgtac gtgttttaag
     5761 tgacatatag agtagaggaa ctgatggaaa tacgatttgc ataatgtaaa atgatatata
     5821 ttaaaatata ggctacagta ggtaacgtgg tttacatata acgttactgt gttaatgtag
     5881 tgctcagtta ctcgtgttga cagttattat tagtgcttgc taatttgttt gttaatgctt
     5941 ctcttgcgta tttgattacc ttcaggcgaa tctaaaacat atcagatgta gaaacggcag
     6001 aaaaatgttg aagaaaatac gagaatagta gcttttggag cacatagaaa agtacatcag
     6061 ataaagccta ccgtttaaac gcagacgccg ctctcgcact gaatgctggg tactgtagtc
     6121 cttatattgt atgcttaatg tgcgtctcaa cgggaaacta gagttaggaa aactacaatt
     6181 tcctgtggaa aatgtttctg ttttcgggga aacagcgaaa gaaagatatg gttccgcgtt
     6241 tttttcttac tttgaataat aaaataagac ctaactacac acttcgctta ctcttatgct
     6301 aatttgaagc actagcattc tttgatacaa tgataatcat ttaaacatca cagttatact
     6361 acaaatacta tggttaaact atgtctagtg tagtgtaaca ctatggttgc cattgttatc
     6421 tttaagttta agtattatct gtggtaaagc catggttggt ttttataaga ggtaataaaa
     6481 ttaacattgt acaattaaat gactataaat taatgttttt ttttattttt tttacacagc
     6541 taccttcaaa aagggatccc atcgattcga attcaaggag cttgccgcca ccatggggag
     6601 cagcaagagc aagcccaagg accccagcca gcgcggggga gaattcatgt acccttatga
     6661 tgtgccagat tatgcctcta atgaggtatc tgtcatcaaa gaaggctggc tccacaaacg
     6721 tggtgaatac atcaagacct ggaggccacg gtacttcctt ctgaagagtg atggatcttt
     6781 cattgggtat aaggagaggc ccgaggcccc tgaccagacc ttaccccccc tgaacaattt
     6841 ctctgtagca gaatgccagc tgatgaagac tgagaggcca cgacccaaca cctttgtcat
     6901 acgctgcctg cagtggacca cagtcatcga gaggaccttc catgtagact ctccagatga
     6961 gagggaagag tggatgcggg ctatccagat ggtcgccaac agtctgaagc agcggggccc
     7021 aggtgaggac gccatggatt acaagtgtgg ctcccccagt gactcttcca catctgagat
     7081 gatggaggta gctgtcaaca aggcacgggc caaagtgacc atgaatgact tcgattatct
     7141 caaactcctc ggcaagggca ccttcggcaa ggtcattctg gttcgagaga aggccactgg
     7201 ccgctattat gccatgaaga tcctgcgcaa ggaggtcatc attgcaaagg atgaagtcgc
     7261 ccacacagtc acagagagcc gggttctgca gaataccagg caccccttcc ttacagccct
     7321 caagtatgcc ttccagaccc atgaccgcct atgctttgtg atggagtatg ccaacggggg
     7381 tgagctgttt ttccacctct ctcgggagcg agtcttcacg gaggatcggg cgcgctttta
     7441 tggagcagag attgtgtcag ctctggagta tttgcactcg agagatgtgg tgtaccgtga
     7501 catcaagctg gaaaacctta tgttggacaa agatggccac atcaagatca ctgactttgg
     7561 cttgtgcaaa gagggcatca gtgatggagc caccatgaaa accttctgtg gtaccccgga
     7621 gtacttggcg cctgaggtgc tagaggacaa tgactatggg cgagcagtgg actggtgggg
     7681 gctgggtgtg gtcatgtatg agatgatgtg tggccgcctg ccattctaca accaggacca
     7741 cgagcgcctc tttgagctca ttcttatgga ggagatccgc ttcccgcgca cactcgggcc
     7801 agaggccaag tccctgctgg ctggactgct gaagaaggac ccaaagcaga ggctcggcgg
     7861 aggtcccagt gatgcgaagg aggtcatgga gcatagattc ttcctcagca tcaactggca
     7921 ggacgtggta cagaaaaagc tcctgccacc cttcaaacct caggtcactt cagaagtgga
     7981 cacaaggtac tttgatgacg agttcaccgc ccagtccatc acaatcacac ccccagaccg
     8041 atatgacagc ctggacccgc tggaactgga ccagcggacg cacttccccc agttctccta
     8101 ctcagccagc atccgagagt gagcagccct ctgccaccac aggacacaag catggccgtc
     8161 atccactgcc tgggtggctt tttaaaaaaa aaaaaaaaaa ggaattctgc agatcctctc
     8221 gagcctctag cttatcgatg atgatccaga catgataaga tacattgatg agtttggaca
     8281 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
     8341 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt
     8401 tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa
     8461 atgtggtatg gctgattatg atcctctaga tcagatccac tagttctaga gcggccatta
     8521 ccctgttatc cctaggccgc caccgcggtg gagctccaat tcgccctata gtgagtcgta
     8581 ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     8641 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     8701 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatgga aattgtaagc
     8761 gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa
     8821 taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt
     8881 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg
     8941 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt
     9001 ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga
     9061 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg
     9121 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg
     9181 cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     9241 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     9301 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     9361 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     9421 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     9481 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     9541 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     9601 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     9661 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     9721 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     9781 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     9841 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     9901 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     9961 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
    10021 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
    10081 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
    10141 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
    10201 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
    10261 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
    10321 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
    10381 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
    10441 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
    10501 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
    10561 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
    10621 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
    10681 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
    10741 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
    10801 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
    10861 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
    10921 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
    10981 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
    11041 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
    11101 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
    11161 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
    11221 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
    11281 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
    11341 acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa
    11401 caaaagctgg gtaccgggcc ccccctcgaa ttaccctgtt atccctatcg agcaaaatca
    11461 atcctaatgt aaaatagtat acgttcatca ttataatgca aacactgtag aactgttgtg
    11521 ttgtgccttg catttcattc tcagtttctt atcgtctgtc tctttttacg acagagctag
    11581 aaaaaactag ccttcatttg ccatcgccgt atgcaaatcc actccagaac tcaacaggtt
    11641 tgttctgcgg cgggtgtaag tgtgaacacc ggcatgtaag tgcgcttgag caggagaata
    11701 aagcgctcag agacaccgga ggaatccttt attttgcgtt gcgcctcagg ggattcgctg
    11761 agccttctct tcatggccct gctcttcaga cacaggtgct ccgctgtttg tgtttt
//
