LOCUS       sequence-46421-e        1097 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Marchetto MC, Muotri AR, Mu Y, Smith AM, Cezar GG, Gage FH
  TITLE     Non-cell-autonomous effect of human SOD1 G37R astrocytes on motor 
            neurons derived from human embryonic stem cells.
  JOURNAL   Cell Stem Cell. 2008 Dec 4;3(6):649-57.
  PUBMED    19041781
REFERENCE   2  (bases 1 to 1097)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell."; date: "2008-12-4"; volume: "3(6)"; pages: "649-57"
COMMENT     Sequence Label: Lenti-Hb9-RFP
FEATURES             Location/Qualifiers
     source          1..1097
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(154..834)
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MVASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGTLIYKVKFRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFL"
     primer_bind     complement(216..239)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(414..433)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     631..651
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     683..701
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     promoter        complement(910..948)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
ORIGIN
        1 gntntgtatt gctacttgtg attgctccat gtttttccag gtttaaactc gagacgttaa
       61 cgggcccagg cggccaccgg tggatcctct agagtttaaa ctgattatga tctagagtcg
      121 cggccgccag tgtgatggat atctgcagaa ttcctacagg aacaggtggt ggcggccctc
      181 ggagcgctcg tactgttcca cgatggtgta gtcctcgttg tgggaggtga tgtccagctt
      241 ggtgtccacg tagtagtagc cgggcagctg cacgggcttc ttggccatgt agatggtctt
      301 gaactccacc aggtagtggc cgccgtcctt cagcttcagg gcctggtgga tctcgccctt
      361 cagcacgccg tcgcgggggt acaggcgctc ggtggaggcc tcccagccca tggtcttctt
      421 ctgcattacg gggccgtcgg gggggaagtt ggtgccgcgg aacttcacct tgtagatcag
      481 cgtgccgtcc tgcagggagg agtcctgggt cacggtcacc acgccgccgt cctcgaagtt
      541 catcacgcgc tcccacttga agccctcggg gaaggacagc ttcttgtagt cggggatgtc
      601 ggcggggtgc ttcacgtacg ccttggagcc gtactggaac tggggggaca ggatgtccca
      661 ggcgaagggc agggggccgc ccttggtcac cttcagcttg gcggtctggg tgccctcgta
      721 ggggcggccc tcgccctcgc cctcgatctc gaactcgtgg ccgttcacgg agccctccat
      781 gcgcaccttg aagcgcatga actctttgat gacgtcctcg gaggaggcca ccatcggatc
      841 ccgggcccgc ggtaccgtcg actgcanaat tcgaagcttg gcgcgccggg atctgcggtt
      901 cactaaacca gctctgctta tatagacctc ccaccgtaca cgcctaccga tcttactgca
      961 aattcattgt ataggagctt catatcatgg gcaggacaag aacagagnca ggctccagcn
     1021 gctgtgcagt cctcttcccc actcaactac ccactgcagg ggctggttag tcatcaaana
     1081 agggnctgct ttgangt
//
