LOCUS       sequence-464218-       10712 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  psPAX2-D64V is derived from psPAX2 plasmid. It is a packaging 
            plasmid for making Integrase deficient lentiviral vectors since has 
            a point mutation in the integrase gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10712)
  AUTHORS   Certo MT, Ryu BY, Annis JE, Garibov M, Jarjour J, Rawlings DJ, 
            Scharenberg AM
  TITLE     Tracking genome engineering outcome at individual DNA breakpoints.
  JOURNAL   Nat Methods. 2011 Jul 10;8(8):671-6. doi: 10.1038/nmeth.1648.
  PUBMED    21743461
REFERENCE   2  (bases 1 to 10712)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-07-10- 10"; volume: "8"; 
            issue: "8"; pages: "671-6"
COMMENT     Sequence Label: psPAX2-D64V
FEATURES             Location/Qualifiers
     source          1..10712
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(57..1559)
                     /codon_start=1
                     /gene="gag"
                     /product="gag protein from human immunodeficiency virus 1"
                     /label=HIV-1 gag
                     /translation="MGARASVLSGGELDRWEKIRLRPGGKKKYKLKHIVWASRELERFA
                     VNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTVATLYCVHQRIEIKDTKEALDKI
                     EEEQNKSKKKAQQAAADTGHSNQVSQNYPIVQNIQGQMVHQAISPRTLNAWVKVVEEKA
                     FSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRVHPVHAGP
                     IAPGQMREPRGSDIAGTTSTLQEQIGWMTHNPPIPVGEIYKRWIILGLNKIVRMYSPTS
                     ILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPG
                     ATLEEMMTACQGVGGPGHKARVLAEAMSQVTNPATIMIQKGNFRNQRKTVKCFNCGKEG
                     HIAKNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQSRPEPTAP
                     PEESFRFGEETTTPSQKQEPIDKELYPLASLRSLFGSDPSSQ"
     primer_bind     complement(1638..1657)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          1665..2681
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(1719..1741)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     promoter        complement(2682..2960)
                     /label=chicken beta-actin promoter
     enhancer        2962..3341
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3372..3476
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3477..3545,3546..4337)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3695..3714)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4508..5096
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4997..5016
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5250..5267
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    5335..5469
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5359..5378)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5413..5432
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(5475..5670)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5489..5624
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    5729..5750
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5765..5770,5771..5788,5789..5795)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5803..5819
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5808..5830
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5827..5843
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5827..5843
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6186..6205
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    6204..6259
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     6305..6324
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(6609..6635)
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             complement(6929..6973)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7158..7391
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             complement(join(7965..10712,1..264))
                     /codon_start=1
                     /gene="pol"
                     /product="pol protein from human immunodeficiency virus 1"
                     /label=HIV-1 pol
                     /translation="FFREDLAFPQGKAREFSSEQTRANSPTRRELQVWGRDNNSLSEAG
                     ADRQGTVSFSFPQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMNLPGRWKPKMIG
                     GIGGFIKVRQYDQILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNFPISPIETV
                     PVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKD
                     STKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKQKKSVTVLDVGDAYFSVPLDKDFRK
                     YTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQCSMTKILEPFRKQNPDIVIYQYMD
                     DLYVGSDLEIGQHRTKIEELRQHLLRWGFTTPDKKHQKEPPFLWMGYELHPDKWTVQPI
                     VLPEKDSWTVNDIQKLVGKLNWASQIYAGIKVRQLCKLLRGTKALTEVVPLTEEAELEL
                     AENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMKGAHT
                     NDVKQLTEAVQKIATESIVIWGKTPKFKLPIQKETWEAWWTEYWQATWIPEWEFVNTPP
                     LVKLWYQLEKEPIIGAETFYVDGAANRETKLGKAGYVTDRGRQKVVPLTDTTNQKTELQ
                     AIHLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVSQIIEQLIKKEKVYLAWVPAH
                     KGIGGNEQVDKLVSAGIRKVLFLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVA
                     SCDKCQLKGEAMHGQVDCSPGIWQLVCTHLEGKVILVAVHVASGYIEAEVIPAETGQET
                     AYFLLKLAGRWPVKTVHTDNGSNFTSTTVKAACWWAGIKQEFGIPYNPQSQGVIESMNK
                     ELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQK
                     QITKIQNFRVYYRDSRDPVWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQ
                     MAGDDCVASRQDED"
     misc_feature    8163..8280
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 tactgtatca tctgctcctg tatctaatag agcttccttt aattgccccc ctatctttat
       61 tgtgacgagg ggtcgctgcc aaagagtgat ctgagggaag ctaaaggata cagttccttg
      121 tctatcggct cctgcttctg agagggagtt gttgtctctt ccccaaacct gaagctctct
      181 tctggtgggg ctgttggctc tggtctgctc tgaagaaaat tccctggcct tcccttgtgg
      241 gaaggccaga tcttccctaa aaaattagcc tgtctctcag tacaatcttt catttggtgt
      301 ccttcctttc cacatttcca acagcccttt ttcctagggg ccctgcaatt tttggctatg
      361 tgcccttctt tgccacaatt gaaacactta acagtctttc tttggttcct aaaattgcct
      421 ttctgtatca ttatggtagc tggatttgtt acttggctca ttgcttcagc caaaactctt
      481 gctttatggc cgggtccccc cactccctga catgctgtca tcatttcttc tagtgtcgct
      541 cctggtccca atgcttttaa aatagtctta caatctgggt tcgcattttg gaccaacaag
      601 gtttctgtca tccaattttt tacctcttgt gaagcttgct cggctcttag agttttatag
      661 aatcggtcta catagtctct aaagggttcc tttggtcctt gtcttatgtc cagaatgctg
      721 gtagggctat acattcttac tattttattt aatcccagga ttatccatct tttatagatt
      781 tctcctactg ggataggtgg attatgtgtc atccatccta tttgttcctg aagggtacta
      841 gtagttcctg ctatgtcact tccccttggt tctctcatct ggcctggtgc aataggccct
      901 gcatgcactg gatgcactct atcccattct gcagcttcct cattgatggt ctcttttaac
      961 atttgcatgg ctgcttgatg tccccccact gtgtttagca tggtgtttaa atcttgtggg
     1021 gtggctcctt ctgataatgc tgaaaacatg ggtatcactt ctgggctgaa agccttctct
     1081 tctactactt ttacccatgc atttaaagtt ctaggtgata tggcctgatg taccatttgc
     1141 ccctggatgt tctgcactat agggtaattt tggctgacct gattgctgtg tcctgtgtca
     1201 gctgctgctt gctgtgcttt tttcttactt ttgttttgct cttcctctat cttgtctaaa
     1261 gcttccttgg tgtcttttat ctctatcctt tgatgcacac aatagagggt tgctactgta
     1321 ttatataatg atctaagttc ttctgatcct gtctgaaggg atggttgtag ctgtcccagt
     1381 atttgtctac agccttctga tgtttctaac aggccaggat taactgcgaa tcgttctagc
     1441 tccctgcttg cccatactat atgttttaat ttatattttt tctttccccc tggccttaac
     1501 cgaatttttt cccatcgatc taattctccc ccgcttaata ctgacgctct cgcacccatc
     1561 tctcaccagt cgccgcccct cgcctcttgc cgtgcgcgtc aacgcggccc gaattctttg
     1621 ccaaaatgat gagacagcac aataaccagc acgttgccca ggagctgtag gaaaaagaag
     1681 aaggcatgaa catggttagc agaggctcta gagccgccgg tcacacgcca gaagccgaac
     1741 cccgccctgc cccgtccccc ccgaaggcag ccgtcccccc gcggacagcc ccgaggctgg
     1801 agagggagaa ggggacggcg gcgcggcgac gcacgaaggc cctccccgcc catttccttc
     1861 ctgccggcgc cgcaccgctt cgccccgcgc ccgctagagg gggtgcggcg gcgcctccca
     1921 gatttcggct ccgcacagat ttgggacaaa ggaagtccct gcgccctctc gcacgattac
     1981 cataaaaggc aatggctgcg gctcgccgcg cctcgacagc cgccggcgct ccgggggccg
     2041 ccgcgcccct cccccgagcc ctccccggcc cgaggcggcc ccgccccgcc cggcaccccc
     2101 acctgccgcc accccccgcc cggcacggcg agccccgcgc cacgccccgt acggagcccc
     2161 gcacccgaag ccgggccgtg ctcagcaact cggggagggg ggtgcagggg gggttgcagc
     2221 ccgaccgacg cgcccacacc ccctgctcac ccccccacgc acacaccccg cacgcagcct
     2281 ttgttcccct cgcagccccc cccgcaccgc ggggcaccgc ccccggccgc gctcccctcg
     2341 cgcacactgc ggagcgcaca aagccccgcg ccgcgcccgc agcgctcaca gccgccgggc
     2401 agcgcggagc cgcacgcggc gctccccacg cacacacaca cgcacgcacc ccccgagccg
     2461 ctccccccgc acaaagggcc ctcccggagc ccctcaaggc tttcacgcag ccacagaaaa
     2521 gaaacaagcc gtcattaaac caagcgctaa ttacagcccg gaggagaagg gccgtcccgc
     2581 ccgctcacct gtgggagtaa cgcggtcagt cagagccggg gcgggcggcg cgaggcggcg
     2641 gcggagcggg gcacggggcg aaggcagcgc gcagcgactc ccgcccgccg cgcgcttcgc
     2701 tttttatagg gccgccgccg ccgccgcctc gccataaaag gaaactttcg gagcgcgccg
     2761 ctctgattgg ctgccgccgc acctctccgc ctcgccccgc cccgcccctc gccccgcccc
     2821 gccccgcctg gcgcgcgccc cccccccccc cccgccccca tcgctgcaca aaataattaa
     2881 aaaataaata aatacaaaat tgggggtggg gagggggggg gagatgggga gagtgaagca
     2941 gaacgtgggg ctcacctcga ccatggtaat agcgatgact aatacgtaga tgtactgcca
     3001 agtaggaaag tcccataagg tcatgtactg ggcataatgc caggcgggcc atttaccgtc
     3061 attgacgtca atagggggcg tacttggcat atgatacact tgatgtactg ccaagtgggc
     3121 agtttaccgt aaatactcca cccattgacg tcaatggaaa gtccctattg gcgttactat
     3181 gggaacatac gtcattattg acgtcaatgg gcgggggtcg ttgggcggtc agccaggcgg
     3241 gccatttacc gtaagttatg taacgcggaa ctccatatat gggctatgaa ctaatgaccc
     3301 cgtaattgat tactattaat aactagtcaa taatcaatgt cgacccaggt ggcacttttc
     3361 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     3421 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     3481 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     3541 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     3601 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     3661 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     3721 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     3781 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     3841 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     3901 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     3961 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     4021 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     4081 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     4141 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     4201 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     4261 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     4321 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     4381 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     4441 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     4501 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     4561 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     4621 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     4681 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     4741 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     4801 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     4861 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     4921 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     4981 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     5041 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     5101 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     5161 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     5221 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     5281 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agcggatcca
     5341 gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa
     5401 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
     5461 aaacaagtta gctttttgca aaagcctagg cctccaaaaa agcctcctca ctacttctgg
     5521 aatagctcag aggccgaggc ggcctcggcc tctgcataaa taaaaaaaat tagtcagcca
     5581 tggggcggag aatgggcgga actgggcgga gttaggggcg ggatgggcgg agttaggggc
     5641 gggactatgg ttgctgacta attgagatgc ggatccgctg gcacgacagg tttcccgact
     5701 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
     5761 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
     5821 ttcacacagg aaacagctat gaccatgatt acgccaagct tgggctgcag gtcgagggat
     5881 ctccataaga gaagagggac agctatgact gggagtagtc aggagaggag gaaaaatctg
     5941 gctagtaaaa catgtaagga aaattttagg gatgttaaag aaaaaaataa cacaaaacaa
     6001 aatataaaaa aaatctaacc tcaagtcaag gcttttctat ggaataagga atggacagca
     6061 gggggctgtt tcatatactg atgacctctt tatagccaac ctttgttcat ggcagccagc
     6121 atatgggcat atgttgccaa actctaaacc aaatactcat tctgatgttt taaatgattt
     6181 gccctcccat atgtccttcc gagtgagaga cacaaaaaat tccaacacac tattgcaatg
     6241 aaaataaatt tcctttatta gccagaagtc agatgctcaa ggggcttcat gatgtcccca
     6301 taatttttgg cagagggaaa aagatctgct agctatagtt ctagaggtac cggttgtttc
     6361 gagcttatag caaaatcctt tccaagccct gtcttattct tctaggtatg tggcgaatag
     6421 ctctacaagc tccttgtact acttctataa ccctatctgt cccctcagct actgctatgg
     6481 ctgtggcatt gagcaagcta acagcactat tctttagctc ctgactccaa tattgtagga
     6541 gattccacca atatttgagg gcttcccacc ccctgcgtcc cagaagttcc acaatcctcg
     6601 ttacaatcaa gagtaagtct ctcaagcggt ggtagctgaa gaggcacagg ctccgcagat
     6661 cgtcccagat aagtgccaag gatccgttca ctaatcgaat ggatctgtct ctgtctctct
     6721 ctccaccttc ttcttctatt ccttcgggcc tgtcgggtcc cctcggggtt gggaggtggg
     6781 tctgaaacga taatggtgaa tatccctgcc taactctatt cactatagaa agtacagcaa
     6841 aaactattct taaacctacc aagcctccta ctatcattat gaataatttt atataccaca
     6901 gccaatttgt tatgttaaac caattccaca aacttgccca tttatctaat tccaataatt
     6961 cttgttcatt cttttcttgc tggttttgcg attcttcaat taaggagtgt attaagcttg
     7021 tgtaattgtt aatttctctg tcccactcca tccaggtcgt gtgattccaa atctgttcca
     7081 gagatttatt actccaacta gcattccaag gcacagcagt ggtgcaaatg agttttccag
     7141 agcaacccca aatccccagg agctgttgat cctttaggta tctttccaca gccaggattc
     7201 ttgcctggag ctgcttgatg ccccagactg tgagttgcaa cagatgctgt tgcgcctcaa
     7261 tagccctcag caaattgttc tgctgctgca ctataccaga caataattgt ctggcctgta
     7321 ccgtcagcgt cattgacgct gcgcccatag tgcttcctgc tgctcccaag aacccaagga
     7381 acaaagctcc tattcccact gctctttttt ctctctgcac cactcttctc tttgccttgg
     7441 tgggtgctac tcctaatggt tcaattttta ctactttata tttatataat tcacttctcc
     7501 aattgtccct catatctcct cctccaggtc tgaagatcag cggccatttt cctatattct
     7561 atgattacta tggaccacac aactattgct attattattg ctactactaa tgctactatt
     7621 gctactatta taggttgcat tacatgtact acttactgct ttgatagaga agcttgatga
     7681 gtctgactgt tctgatgagc tcttcgtcgc tgtctccgct tcttcctgcc ataggagatg
     7741 cctaaggctt ttgtcatgaa acaaacttgg caatgaaagc aacacttttt acaatagcaa
     7801 ttggtacaag cagttttagg ctgacttcct ggatgcttcc agggctctag tctaggatct
     7861 actggctcca tttcttgctc tcctctgtcg agtaacgcct attctgctat gtcgacaccc
     7921 aattctgaaa tggataaaca gcagttgttg cagaattcca tgtgttaatc ctcatcctgt
     7981 ctacttgcca cacaatcatc acctgccatc tgttttccat aatccctgat gatctttgct
     8041 tttcttcttg gcactacttt tatgtcacta ttatcttgta ttactactgc cccttcacct
     8101 ttccagagga gctttgctgg tcctttccaa actggatctc tgctgtccct gtaataaacc
     8161 cgaaaatttt gaatttttgt aatttgtttt tgtaattctt tagtttgtat gtctgttgct
     8221 attatgtcta ctattctttc ccctgcactg taccccccaa tccccccttt tcttttaaaa
     8281 ttgtggatga atactgccat ttgtactgct gtcttaagat gttcagcctg atctcttacc
     8341 tgtcctataa ttttctttaa ttctttattc atagattcta ttactccttg actttgggga
     8401 ttgtagggaa tgccaaattc ctgcttgatc cccgcccacc aacaggcggc cttaactgta
     8461 gtactggtga aattgctgcc attgtctgta tgtactgttt ttactggcca tcttcctgct
     8521 aattttaaga ggaagtatgc tgtttcttgc cctgtctctg ctggaattac ttctgcttct
     8581 atatatccac tggctacatg aactgctacc aagataactt ttccttctaa atgtgtacaa
     8641 actagctgcc atattcctgg gctacagtct acttgtccat gcatggcttc cccttttagc
     8701 tgacatttat cacagctggc tactatttct tttgctacta caggtggtag gttaaaatca
     8761 ctagccattg ctctccaatt actgtgatat ttctcatgtt cttcttgggc cttatctatt
     8821 ccatctaaaa atagtacttt cctgattcca gcactgacca atttatctac ttgttcattt
     8881 cctccaattc ctttgtgtgc tggtacccat gccaggtaga ctttttcctt ttttattaac
     8941 tgctctatta tttgactgac taactctgat tcactcttat ctggttgtgc ttgaatgatt
     9001 cccaatgcat attgtgagtc tgtcactatg tttacttcta atcccgaatc ctgcaaagct
     9061 agatgaattg cttgtaactc agtcttctga tttgttgtgt ccgttagggg gacaactttt
     9121 tgtcttcctc tgtcagttac atatcctgct tttcctaatt tagtttccct attggctgcc
     9181 ccatctacat agaaagtttc tgctcctatt atgggttctt tctctaactg gtaccataac
     9241 ttcactaagg gaggggtatt gacaaactcc cactcaggaa tccaggtggc ttgccaatac
     9301 tctgtccacc atgcttccca tgtttccttt tgtatgggta atttaaattt aggagtcttt
     9361 ccccatatta ctatgctttc tgtggctatt ttttgtactg cctctgttaa ttgtttcaca
     9421 tcattagtgt gggcaccctt cattcttgca tactttcctg ttttcagatt tttaaatggc
     9481 tcttgataaa tttgatatgt ccattggcct tgcccctgct tctgtatttc tgctattaag
     9541 tcttttgatg ggtcataata cactccatgt accggttctt ttagaatctc cctgttttct
     9601 gccagttcta gctctgcttc ttctgttagt ggtactactt ctgttagtgc tttggttccc
     9661 ctaagaagtt tacataattg ccttacttta atccctgcat aaatctgact tgcccaattc
     9721 aattttccca ctaatttctg tatgtcattg acagtccagc tgtccttttc tggcagcact
     9781 ataggctgta ctgtccattt atcaggatgg agttcataac ccatccaaag gaatggaggt
     9841 tctttctgat gttttttgtc tggtgtggta aatccccacc tcaacagatg ttgtctcagt
     9901 tcctctattt ttgttctatg ctgccctatt tctaagtcag atcctacata caaatcatcc
     9961 atgtattgat agatgactat gtctggattt tgttttctaa aaggctctaa gatttttgtc
    10021 atgctacact ggaatattgc tggtgatcct ttccatccct gtggaagcac attgtactga
    10081 tatctaatcc ctggtgtctc attgtttata ctaggtatgg taaatgcagt atacttcctg
    10141 aagtctttat ctaagggaac tgaaaaatat gcatcgccca catccagtac tgttactgat
    10201 tttttctgtt ttaaccctgc aggatgtggt attcctaatt gaacttccca gaaatcttga
    10261 gttctcttat taagttctct gaaatctact aattttctcc atttagtact gtcttttttc
    10321 tttatggcaa atactggagt attgtatgga ttttcaggcc caatttttga aatttttcct
    10381 tccttttcca tttctgtaca aatttctact aatgctttta ttttttcttc tgtcaatggc
    10441 cattgtttaa cttttgggcc atccattcct ggctttaatt ttactggtac agtctcaata
    10501 ggactaatgg gaaaatttaa agtgcagcca atctgagtca acagatttct tccaattatg
    10561 ttgacaggtg taggtcctac taatactgta cctatagctt tatgtccgca gatttctatg
    10621 agtatctgat catactgtct tactttgata aaacctccaa ttccccctat catttttggt
    10681 ttccatcttc ctggcaaatt catttcttct aa
//
