LOCUS       sequence-464233-        5419 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  transient expresses PD-L2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5419)
  AUTHORS   Chou WC, Chen WT, Kuo CT, Chang YM, Lu YS, Li CW, Hung MC, Shen CY
  TITLE     Genetic insights into carbohydrate sulfotransferase 8 and its impact
            on the immunotherapy efficacy of cancer.
  JOURNAL   Cell Rep. 2024 Jan 23;43(1):113641. doi: 
            10.1016/j.celrep.2023.113641. Epub 2023 Dec 30.
  PUBMED    38165805
REFERENCE   2  (bases 1 to 5419)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2024-01-23"; volume: "43(1):113641. doi"; pages: " 
            10.1016/j.celrep.2023.113641. Epub 2023 Dec 30"
COMMENT     Sequence Label: pXJ-His-PDCD1LG2
FEATURES             Location/Qualifiers
     source          1..5419
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        2..305
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        306..509
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     460..480
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     506..530
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          671..1243
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     1251..1270
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             2161..2190
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     promoter        complement(2219..2237)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    2511..2632
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2548..2567)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2602..2621
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2660..2677)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2831..3419)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2911..2930)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3590..4381,4382..4450))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4213..4232
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4451..4555)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4623..4641
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4679..4701)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4801..4820
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(4887..5342)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5024..5045)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5236..5255
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ccgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
       61 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
      121 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
      181 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
      241 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
      301 ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg
      361 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac
      421 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg
      481 tacggtggga ggtctatata agcagagctc gtttagtgaa ccgtcagatc gcctggagac
      541 gccatccacg ctgttttgac ctccatagaa gacaccggga ccgatccagc ctccgcggcc
      601 gggaacggtg cattggaacg gacccctagg cttttgcaaa aagctccgga tcgatcctga
      661 gaacttcagg gtgagtttgg ggacccttga ttgttctttc tttttcgcta ttgtaaaatt
      721 catgttatat ggagggggca aagttttcag ggtgttgttt agaatgggaa gatgtccctt
      781 gtatcaccat ggaccctcat gataattttg tttctttcac tttctactct gttgacaacc
      841 attgtctcct cttattttct tttcattttc tgtaactttt tcgttaaact ttagcttgca
      901 tttgtaacga atttttaaat tcacttttgt ttatttgtca gattgtaagt actttctcta
      961 atcacttttt tttcaaggca atcagggtat attatattgt acttcagcac agttttagag
     1021 aacaattgtt ataattaaat gataaggtag aatatttctg catataaatt ctggctggcg
     1081 tggaaatatt cttattggta gaaacaacta catcctggtc atcatcctgc ctttctcttt
     1141 atggttacaa tgatatacac tgtttgagat gaggataaaa tactctgagt ccaaaccggg
     1201 cccctctgct aaccatgttc atgccttctt ctttttccta cagctcctgg gcaacgtgct
     1261 ggttattgtg ctgtctcatc attttggcaa agaattgtaa tacgactcac taagggcggc
     1321 cgcggatccg ccaccatgat cttcctcctg ctaatgttga gcctggaatt gcagcttcac
     1381 cagatagcag ctttattcac agtgacagtc cctaaggaac tgtacataat agagcatggc
     1441 agcaatgtga ccctggaatg caactttgac actggaagtc atgtgaacct tggagcaata
     1501 acagccagtt tgcaaaaggt ggaaaatgat acatccccac accgtgaaag agccactttg
     1561 ctggaggagc agctgcccct agggaaggcc tcgttccaca tacctcaagt ccaagtgagg
     1621 gacgaaggac agtaccaatg cataatcatc tatggggtcg cctgggacta caagtacctg
     1681 actctgaaag tcaaagcttc ctacaggaaa ataaacactc acatcctaaa ggttccagaa
     1741 acagatgagg tagagctcac ctgccaggct acaggttatc ctctggcaga agtatcctgg
     1801 ccaaacgtca gcgttcctgc caacaccagc cactccagga cccctgaagg cctctaccag
     1861 gtcaccagtg ttctgcgcct aaagccaccc cctggcagaa acttcagctg tgtgttctgg
     1921 aatactcacg tgagggaact tactttggcc agcattgacc ttcaaagtca gatggaaccc
     1981 aggacccatc caacttggct gcttcacatt ttcatcccct cctgcatcat tgctttcatt
     2041 ttcatagcca cagtgatagc cctaagaaaa caactctgtc aaaagctgta ttcttcaaaa
     2101 gacacaacaa aaagacctgt caccacaaca aagagggaag tgaacagtgc tatcctcgag
     2161 caccatcacc atcaccatca ccatcaccat tagtaccagg taagtgtacc caattcgccc
     2221 tatagtgagt cgtattacaa ttcactcgat cgcccttccc aacagttgcg cagcctgaat
     2281 ggcgaatgga gatccaattt ttaagtgtat aatgtgttaa actactgatt ctaattgttt
     2341 gtgtatttta gattcacagt cccaaggctc atttcaggcc cctcagtcct cacagtctgt
     2401 tcatgatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc
     2461 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
     2521 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     2581 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct taacgcgcga
     2641 ctctagactc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     2701 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     2761 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     2821 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     2881 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     2941 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     3001 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     3061 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     3121 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     3181 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     3241 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     3301 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     3361 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     3421 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     3481 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     3541 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     3601 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     3661 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     3721 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     3781 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     3841 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     3901 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     3961 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     4021 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     4081 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     4141 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     4201 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     4261 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     4321 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     4381 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     4441 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     4501 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     4561 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
     4621 aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc
     4681 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
     4741 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg
     4801 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
     4861 gcgtaaggag aaaataccgc atcaggaaat tgtaagcgtt aatattttgt taaaattcgc
     4921 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
     4981 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag
     5041 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
     5101 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc
     5161 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
     5221 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt
     5281 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc
     5341 gtccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
     5401 ctattacgcc agctagagt
//
