LOCUS       sequence-464234-        6457 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  transient expresses TCF7L2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6457)
  AUTHORS   Chou WC, Chen WT, Shen CY
  TITLE     A common variant in 11q23.3 associated with hyperlipidemia is 
            mediated by the binding and regulation of GATA4.
  JOURNAL   NPJ Genom Med. 2022 Jan 19;7(1):4. doi: 10.1038/s41525-021-00279-5.
  PUBMED    35046404
REFERENCE   2  (bases 1 to 6457)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "NPJ Genom 
            Med."; date: "2022-01-19"; volume: "7(1):4. doi"; pages: " 
            10.1038/s41525-021-00279-5"
COMMENT     Sequence Label: pXJ-Myc-TCF7L2
FEATURES             Location/Qualifiers
     source          1..6457
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        2..305
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        306..509
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     460..480
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     506..530
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          671..1243
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     1251..1270
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        1298..1316
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1337..1366
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     promoter        complement(3257..3275)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    3549..3670
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3586..3605)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3640..3659
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3698..3715)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3869..4457)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3949..3968)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4628..5419,5420..5488))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5251..5270
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5489..5593)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5661..5679
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5717..5739)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5839..5858
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(5925..6380)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6062..6083)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6274..6293
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ccgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
       61 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
      121 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
      181 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
      241 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
      301 ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg
      361 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac
      421 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg
      481 tacggtggga ggtctatata agcagagctc gtttagtgaa ccgtcagatc gcctggagac
      541 gccatccacg ctgttttgac ctccatagaa gacaccggga ccgatccagc ctccgcggcc
      601 gggaacggtg cattggaacg gacccctagg cttttgcaaa aagctccgga tcgatcctga
      661 gaacttcagg gtgagtttgg ggacccttga ttgttctttc tttttcgcta ttgtaaaatt
      721 catgttatat ggagggggca aagttttcag ggtgttgttt agaatgggaa gatgtccctt
      781 gtatcaccat ggaccctcat gataattttg tttctttcac tttctactct gttgacaacc
      841 attgtctcct cttattttct tttcattttc tgtaactttt tcgttaaact ttagcttgca
      901 tttgtaacga atttttaaat tcacttttgt ttatttgtca gattgtaagt actttctcta
      961 atcacttttt tttcaaggca atcagggtat attatattgt acttcagcac agttttagag
     1021 aacaattgtt ataattaaat gataaggtag aatatttctg catataaatt ctggctggcg
     1081 tggaaatatt cttattggta gaaacaacta catcctggtc atcatcctgc ctttctcttt
     1141 atggttacaa tgatatacac tgtttgagat gaggataaaa tactctgagt ccaaaccggg
     1201 cccctctgct aaccatgttc atgccttctt ctttttccta cagctcctgg gcaacgtgct
     1261 ggttattgtg ctgtctcatc attttggcaa agaattgtaa tacgactcac tatagggcga
     1321 attcaccatg gcagaggagc aaaagctcat ttctgaagag gacttgttgc ggaaacatat
     1381 gggatccatg ccgcagctga acggcggtgg aggggatgac ctaggcgcca acgacgaact
     1441 gatttccttc aaagacgagg gcgaacagga ggagaagagc tccgaaaact cctcggcaga
     1501 gagggattta gctgatgtca aatcgtctct agtcaatgaa tcagaaacga atcaaaacag
     1561 ctcctccgat tccgaggcgg aaagacggcc tccgcctcgc tccgaaagtt tccgagacaa
     1621 atcccgggaa agtttggaag aagcggccaa gaggcaagat ggagggctct ttaaggggcc
     1681 accgtatccc ggctacccct tcatcatgat ccccgacctg acgagcccct acctccccaa
     1741 cggatcgctc tcgcccaccg cccgaaccct ccattttcag tccggcagca cacattactc
     1801 tgcgtacaaa acgattgaac accagattgc agttcagtat ctccagatga aatggccact
     1861 gcttgatgtc caggcaggga gcctccagag tagacaagcc ctcaaggatg cccggtcccc
     1921 atcaccggca cacattgtct ctaacaaagt gccagtggtg cagcaccctc accatgtcca
     1981 ccccctcacg cctcttatca cgtacagcaa tgaacacttc acgccgggaa acccacctcc
     2041 acacttacca gccgacgtag accccaaaac aggaatccca cggcctccgc accctccaga
     2101 tatatccccg tattacccac tatcgcctgg caccgtagga caaatccccc atccgctagg
     2161 atggttagta ccacagcaag gtcaaccagt gtacccaatc acgacaggag gattcagaca
     2221 cccctacccc acagctctga ccgtcaatgc ttccatgtcc aggttccctc cccatatggt
     2281 cccaccacat catacgctac acacgacggg cattccgcat ccggccatag tcacaccaac
     2341 agtcaaacag gaatcgtccc agagtgatgt cggctcactc catagttcaa agcatcagga
     2401 ctccaaaaag gaagaagaaa agaagaagcc ccacataaag aaacctctta atgcattcat
     2461 gttgtatatg aaggaaatga gagcaaaggt cgtagctgag tgcacgttga aagaaagcgc
     2521 ggccatcaac cagatccttg ggcggaggtg gcatgcactg tccagagaag agcaagcgaa
     2581 atactacgag ctggcccgga aggagcgaca gcttcatatg caactgtacc ccggctggtc
     2641 cgcgcgggat aactatggaa agaagaagaa gaggaaaagg gacaagcagc cgggagagac
     2701 caatgatgca aatactccaa agaagtgtcg ggcactgttc gggcttgacc gacagacttt
     2761 atggtgcaaa ccgtgcagga gaaaaaaaaa gtgcgttcgc tacatacaag gtgaaggcag
     2821 ctgcctcagc ccaccctctt cagatggaag cttactagat tcgcctcccc cctccccgaa
     2881 cctgctaggc tcccctcccc gagacgccaa gtcacagact gagcagaccc agcctctgtc
     2941 gctgtccctg aagcccgacc ccctggccca cctgtccatg atgcctccgc cacccgccct
     3001 cctgctcgct gaggccaccc acaaggcctc cgccctctgt cccaacgggg ccctggacct
     3061 gcccccagcc gctttgcagc ctgccgcccc ctcctcatca attgcacagc cgtcgacttc
     3121 ttccttacat tcccacagct ccctggccgg gacccagccc cagccgctgt cgctcgtcac
     3181 caagtcttta gaatagctcg agggggggcc cggtacctta attaattaag gtaccaggta
     3241 agtgtaccca attcgcccta tagtgagtcg tattacaatt cactcgatcg cccttcccaa
     3301 cagttgcgca gcctgaatgg cgaatggaga tccaattttt aagtgtataa tgtgttaaac
     3361 tactgattct aattgtttgt gtattttaga ttcacagtcc caaggctcat ttcaggcccc
     3421 tcagtcctca cagtctgttc atgatcataa tcagccatac cacatttgta gaggttttac
     3481 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
     3541 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     3601 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     3661 atgtatctta acgctcgact ctagactctt ccgcttcctc gctcactgac tcgctgcgct
     3721 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     3781 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     3841 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     3901 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     3961 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     4021 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     4081 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     4141 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     4201 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     4261 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     4321 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     4381 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     4441 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     4501 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     4561 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     4621 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     4681 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     4741 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     4801 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     4861 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     4921 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     4981 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     5041 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     5101 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     5161 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     5221 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     5281 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     5341 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     5401 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     5461 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     5521 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     5581 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
     5641 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg
     5701 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
     5761 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
     5821 ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
     5881 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggaaattg taagcgttaa
     5941 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc
     6001 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt
     6061 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa
     6121 aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg
     6181 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg
     6241 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc
     6301 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa
     6361 tgcgccgcta cagggcgcgt ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
     6421 tcggtgcggg cctcttcgct attacgccag ctagagt
//
