LOCUS       sequence-464243-        1960 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression vector with myc-tagged mouse Grhl2 coding 
            sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1960)
  TITLE     GRHL and PGR control WNT4 expression in the mammary gland via 3D 
            looping of conserved and species-specific enhancers
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pGlomyc-Grhl2
FEATURES             Location/Qualifiers
     source          1..1960
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      21..30
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             30..59
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 agatctgatc tagaactagt gccgccatgg agcagaagct gatcagcgag gaggacctgc
       61 agcccgggga tccgatgtca caagagtcgg acaataataa aagactagtg gccttagtgc
      121 ccatgcccag tgaccctccc ttcaacaccc gaagagccta cacaagtgag gatgaggcct
      181 ggaagtcata tctggagaac cccctgactg cggccaccaa ggcgatgatg agcatcaacg
      241 gggacgagga cagtgctgcc gccctgggcc tgctctatga ctactacaag gttcctcgag
      301 acaagagact tctgtctgtg agcaaagcaa gtgacagcca agaagaccag gataaaagaa
      361 actgccttgg caccagtgaa gcccagatca atttgagcgg aggcgagaac agagtgcagg
      421 ttctgaagac tgtcccggtg aacctctgtc taagtcaaga ccacatggag aattcgaagc
      481 gcgagcagta cagtgtatcc atcaccgaga gctctgccgt catccccgtg tcaggcatca
      541 ccgtggtgaa agccgaggat ttcacaccgg tgttcatggc gcccccggtg cactatcccc
      601 gcgcggacag tgaggagcag cgcgtggtta tctttgaaca gactcagtac gacctgccct
      661 ccatagccag ccacagctcc tatctcaagg acgaccagcg cagcacgccg gacagcacct
      721 acagcgagag ctttaaggac ggcgcctcgg agaaatttcg gagtacttct gttggtgctg
      781 acgagtatac atatgaccag acgggaagtg gtacatttca gtacaccctg gaagccacca
      841 aatctctccg tcagaagcag ggggagggcc ccatgaccta cctcaacaaa ggacaattct
      901 atgccataac actcagtgag actggagaca acaaatgctt ccgacacccc atcagcaaag
      961 tcaggagtgt ggtgatggtg gtctttagtg aagacaaaaa ccgagatgag cagctgaaat
     1021 actggaagta ctggcactcc cggcagcaca ctgccaagca gagggtcctt gacattgctg
     1081 attacaagga gagcttcaac accatcggga acattgaaga gatcgcatac aatgctgttt
     1141 ccttcacctg ggatgtgaac gaggaggcaa agatttttat caccgtgaat tgcctgagta
     1201 cagatttctc ctcccaaaag ggtgtaaaag gacttcccct gatgattcag atcgacacgt
     1261 acagctacaa caaccgcagc aataaaccca tccacagagc atactgccag atcaaggtct
     1321 tctgtgacaa gggagcagaa agaaaaatcc gggatgaaga gagaaagcag aacaggaaga
     1381 aagggaaggg ccaggcctct caagcccagt gcaacaactc ctctgatggg aagatggccg
     1441 ccataccgtt acagaagaag agtgacatca cgtacttcaa aaccatgccc gacctgcact
     1501 cacagcctgt gctcttcata ccagatgttc actttgcaaa cctacagagg accggacagg
     1561 tttattacaa cacagacgat gagcgagaag gcagcagcgt ccttgttaag cggatgttca
     1621 ggcccatgga agaggagttt ggtccaacac cgtctaagca gatcaaagaa gaaaacgtaa
     1681 aacgagtgct tttatatgtg aggaaggaga acgatgacgt cttcgatgct ctgatgctga
     1741 aatcacccac ggtgaagggt ctgatggaag cgctgtctga gaagtatggg ctgccagtgg
     1801 agaaaatcac aaagctttat aagaagagca aaaagggcat cctggtcaac atggatgaca
     1861 acatcattga gcactattca aatgaggaca ccttcatcct caacatggag agcatggtgg
     1921 aaggcttcaa gatcacgctg atggagatct gagcggccgc
//
