LOCUS       sequence-464286-        5929 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  transient expresses IRF8 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5929)
  TITLE     Shen Lab plasmids
REFERENCE   2  (bases 1 to 5929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pXJ-Myc-IRF8
FEATURES             Location/Qualifiers
     source          1..5929
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        2..305
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        306..509
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     460..480
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     506..530
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          671..1243
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     1251..1270
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        1298..1316
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1337..1366
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     promoter        complement(2729..2747)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    3021..3142
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3058..3077)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3112..3131
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3170..3187)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3341..3929)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3421..3440)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4100..4891,4892..4960))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4723..4742
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4961..5065)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5133..5151
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5189..5211)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5311..5330
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(5397..5852)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5534..5555)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5746..5765
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ccgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
       61 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
      121 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
      181 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
      241 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
      301 ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg
      361 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac
      421 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg
      481 tacggtggga ggtctatata agcagagctc gtttagtgaa ccgtcagatc gcctggagac
      541 gccatccacg ctgttttgac ctccatagaa gacaccggga ccgatccagc ctccgcggcc
      601 gggaacggtg cattggaacg gacccctagg cttttgcaaa aagctccgga tcgatcctga
      661 gaacttcagg gtgagtttgg ggacccttga ttgttctttc tttttcgcta ttgtaaaatt
      721 catgttatat ggagggggca aagttttcag ggtgttgttt agaatgggaa gatgtccctt
      781 gtatcaccat ggaccctcat gataattttg tttctttcac tttctactct gttgacaacc
      841 attgtctcct cttattttct tttcattttc tgtaactttt tcgttaaact ttagcttgca
      901 tttgtaacga atttttaaat tcacttttgt ttatttgtca gattgtaagt actttctcta
      961 atcacttttt tttcaaggca atcagggtat attatattgt acttcagcac agttttagag
     1021 aacaattgtt ataattaaat gataaggtag aatatttctg catataaatt ctggctggcg
     1081 tggaaatatt cttattggta gaaacaacta catcctggtc atcatcctgc ctttctcttt
     1141 atggttacaa tgatatacac tgtttgagat gaggataaaa tactctgagt ccaaaccggg
     1201 cccctctgct aaccatgttc atgccttctt ctttttccta cagctcctgg gcaacgtgct
     1261 ggttattgtg ctgtctcatc attttggcaa agaattgtaa tacgactcac tatagggcga
     1321 attcaccatg gcagaggagc aaaagctcat ttctgaagag gacttgttgc ggaaacatat
     1381 gggatctatg tgtgaccgga atggtggtcg gcggcttcga cagtggctga tcgagcagat
     1441 tgacagtagc atgtatccag gactgatttg ggagaatgag gagaagagca tgttccggat
     1501 cccttggaaa cacgctggca agcaagatta taatcaggaa gtggatgcct ccatttttaa
     1561 ggcctgggca gtttttaaag ggaagtttaa agaaggggac aaagctgaac cagccacttg
     1621 gaagacgagg ttacgctgtg ctttgaataa gagcccagat tttgaggaag tgacggaccg
     1681 gtcccaactg gacatttccg agccatacaa agtttaccga attgttcctg aggaagagca
     1741 aaaatgcaaa ctaggcgtgg caactgctgg ctgcgtgaat gaagttacag agatggagtg
     1801 cggtcgctct gaaatcgacg agctgatcaa ggagccttct gtggacgatt acatggggat
     1861 gatcaaaagg agcccttccc cgccggaggc ctgtcggagt cagctccttc cagactggtg
     1921 ggcgcagcag cccagcacag gcgtgccgct ggtgacgggg tacaccacct acgacgcgca
     1981 ccattcagca ttctcccaga tggtgatcag cttctactat gggggcaagc tggtgggcca
     2041 ggccaccacc acctgccccg agggctgccg cctgtccctg agccagcctg ggctgcccgg
     2101 caccaagctg tatgggcccg agggcctgga gctggtgcgc ttcccgccgg ccgacgccat
     2161 ccccagcgag cgacagaggc aggtgacgcg gaagctgttc gggcacctgg agcgcggggt
     2221 gctgctgcac agcagccggc agggcgtgtt cgtcaagcgg ctgtgccagg gccgcgtgtt
     2281 ctgcagcggc aacgccgtgg tgtgcaaagg caggcccaac aagctggagc gtgatgaggt
     2341 ggtccaggtc ttcgacacca gccagttctt ccgagagctg cagcagttct ataacagcca
     2401 gggccggctt cctgacggca gggtggtgct gtgctttggg gaagagtttc cggatatggc
     2461 ccccttgcgc tccaaactca ttctcgtgca gattgagcag ctgtatgtcc ggcaactggc
     2521 agaagaggct gggaagagct gtggagccgg ctctgtgatg caggcccccg aggagccgcc
     2581 gccagaccag gtcttccgga tgtttccaga tatttgtgcc tcacaccaga gatcattttt
     2641 cagagaaaac caacagatca ccgtctaact cgaggggggg cccggtacct taattaatta
     2701 aggtaccagg taagtgtacc caattcgccc tatagtgagt cgtattacaa ttcactcgat
     2761 cgcccttccc aacagttgcg cagcctgaat ggcgaatgga gatccaattt ttaagtgtat
     2821 aatgtgttaa actactgatt ctaattgttt gtgtatttta gattcacagt cccaaggctc
     2881 atttcaggcc cctcagtcct cacagtctgt tcatgatcat aatcagccat accacatttg
     2941 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
     3001 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
     3061 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     3121 ccaaactcat caatgtatct taacgcgcga ctctagactc ttccgcttcc tcgctcactg
     3181 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     3241 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     3301 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     3361 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     3421 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     3481 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     3541 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     3601 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     3661 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     3721 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     3781 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     3841 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     3901 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     3961 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     4021 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     4081 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     4141 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     4201 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     4261 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     4321 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     4381 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     4441 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     4501 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     4561 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     4621 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     4681 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     4741 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     4801 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     4861 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     4921 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     4981 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     5041 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag
     5101 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
     5161 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     5221 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     5281 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     5341 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggaaat
     5401 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
     5461 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
     5521 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
     5581 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
     5641 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
     5701 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
     5761 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
     5821 cgccgcgctt aatgcgccgc tacagggcgc gtccattcgc cattcaggct gcgcaactgt
     5881 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctagagt
//
