LOCUS       sequence-464301-        9868 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses CHST8 REP-to-AAA mutant in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9868)
  AUTHORS   Chou WC, Chen WT, Kuo CT, Chang YM, Lu YS, Li CW, Hung MC, Shen CY
  TITLE     Genetic insights into carbohydrate sulfotransferase 8 and its impact
            on the immunotherapy efficacy of cancer.
  JOURNAL   Cell Rep. 2024 Jan 23;43(1):113641. doi: 
            10.1016/j.celrep.2023.113641. Epub 2023 Dec 30.
  PUBMED    38165805
REFERENCE   2  (bases 1 to 9868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2024-01-23"; volume: "43(1):113641. doi"; pages: " 
            10.1016/j.celrep.2023.113641. Epub 2023 Dec 30"
COMMENT     Sequence Label: pLAS2w.Phyg-CHST8 (3A)
FEATURES             Location/Qualifiers
     source          1..9868
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        295..598
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        599..802
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     753..773
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        847..865
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2173..2238
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    2281..2398
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2447..2957
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2876..2894
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2969..3988
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     "
     misc_feature    4019..4607
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4072..4092)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4490..4501)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4679..4912
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4990..5111
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5027..5046)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5081..5100
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5151..5286
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(5307..5325)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5335..5352)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5335..5351)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5344..5366)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5493..5948
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5580..5599)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5790..5811
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5974..6078
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6079..6147,6148..6939)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6297..6316)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7110..7698
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7599..7618
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7852..7869
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7986..8007
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8022..8027,8028..8045,8046..8052)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8060..8076
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8065..8087
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8084..8100
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8084..8100
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8119..8139
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8121..8139
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8167..8393
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8394..8574
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8621..8746
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9239..9472
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9657..9701
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc
       61 cattcgatta gtgaacggat ctcgacggta tcgatcacga gactagcctc gaggatccac
      121 tagtgaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac
      181 gggccagaaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttctagtta
      241 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
      301 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
      361 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
      421 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
      481 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
      541 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
      601 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
      661 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
      721 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
      781 ggaggtctat ataagcagag ctctctggct aactagagaa cccactgctt actggcttat
      841 cgaaattaat acgactcact atagggagac ccaagctggc tagccgccac catgaccctg
      901 cgacctggaa caatgcggct ggcctgcatg ttctcttcca tcctgctgtt cggagctgca
      961 ggcctcctcc tcttcatcag cctgcaggac cctacggagc tcgcccccca gcaggtgcca
     1021 ggaataaagt tcaacatcag gccaaggcag ccccaccacg acctcccacc aggcggctcc
     1081 caggatggtg acttgaagga acccacagag agggtcactc gggacttatc cagtggggcc
     1141 ccgaggggcc gcaacctgcc agcgcctgac cagcctcaac ccccgctgca gaggggaacc
     1201 cgtctgcggc tccgccagcg ccgtcgccgt ctgctcatca agaaaatgcc agctgcggcg
     1261 accatcccgg ccaacagctc ggacgcgccc ttcatccggc cgggacccgg gacgctggat
     1321 ggccgctggg tcagcctgca ccggagccag caggagcgca agcgggtgat gcaggaggcc
     1381 tgcgccaagt accgggcgag cagcagccgc cgggccgtca cgccccgcca cgtgtcccgt
     1441 atcttcgtgg aggaccgcca ccgcgtgctc tactgcgagg tgcccaaggc cggctgctcc
     1501 aattggaagc gggtgctcat ggtgctggcc ggcctggcct cgtccactgc cgacatccag
     1561 cacaacaccg tccactatgg cagcgctctc aagcgcctgg acaccttcga ccgccagggt
     1621 atcttgcacc gtctcagcac ctacaccaag atgctctttg tcgccgcggc cttcgagagg
     1681 ctggtgtccg ccttccgcga caagtttgag caccccaaca gctactatca cccggtcttc
     1741 ggcaaggcca tcctggcccg gtaccgcgcc aatgcctctc gggaggccct gcggaccggc
     1801 tctggggtgc gttttcccga gttcgtccag tacctgctgg acgtgcaccg gcccgtgggg
     1861 atggacattc actgggacca tgtcagccgg ctctgcagcc cctgcctcat cgactacgat
     1921 ttcgtaggca agttcgagag catggaggac gatgccaact tcttcctgag cctcatccgc
     1981 gcgccgcgga acctgacctt cccccggttc aaggaccggc actcgcagga ggcgcggacc
     2041 acagcgagga tcgcccacca gtacttcgcc caactctcgg ccctgcaaag gcagcgcacc
     2101 tacgacttct actacatgga ttacctgatg ttcaactatt ccaagccctt tgcagatctg
     2161 tacggatcca tggactacaa agaccatgac ggtgattata aagatcatga catcgactac
     2221 aaagacgatg acgacaagtg aattctcgac ctcgagacaa atggcagtat tcatccacaa
     2281 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
     2341 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg
     2401 ggtttattac agggacagca gagatccact ttggccgcgg ctcgaggggg ttggggttgc
     2461 gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg cgtggttccg
     2521 ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt cgcagcgtca
     2581 cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct ccgcccctaa
     2641 gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa gccgcacgtc
     2701 tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc cgaccgcgat
     2761 gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc cgggaagggg
     2821 cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc gcgcggtgtt
     2881 ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt gaccgaatca
     2941 ccgacctctc tccccagggg gatccaccat gaaaaagcct gaactcaccg ctacctctgt
     3001 cgagaagttt ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc tctccgaggg
     3061 cgaagaatct cgggctttca gcttcgatgt gggagggcgt ggatatgtcc tgcgggtgaa
     3121 tagctgcgcc gatggtttct acaaagatcg ctatgtttat cggcactttg catccgccgc
     3181 tctccctatt cccgaagtgc ttgacattgg ggagttcagc gagagcctga cctattgcat
     3241 ctcccgccgt gcacagggtg tcaccttgca agacctgcct gaaaccgaac tgcccgctgt
     3301 tctccagccc gtcgccgagg ccatggatgc catcgctgcc gccgatctta gccagaccag
     3361 cgggttcggc ccattcggac ctcaaggaat cggtcaatac actacatggc gcgatttcat
     3421 ctgcgctatt gctgatcccc atgtgtatca ctggcaaact gtgatggacg acaccgtcag
     3481 tgcctccgtc gcccaggctc tcgatgagct gatgctttgg gccgaggact gccccgaagt
     3541 ccggcacctc gtgcacgccg atttcggctc caacaatgtc ctgaccgaca atggccgcat
     3601 aacagccgtc attgactgga gcgaggccat gttcggggat tcccaatacg aggtcgccaa
     3661 catcttcttc tggaggccct ggttggcttg tatggagcag cagacccgct acttcgagcg
     3721 gaggcatccc gagcttgcag gatctcctcg gctccgggct tatatgctcc gcattggtct
     3781 tgaccaactc tatcagagct tggttgacgg caatttcgat gatgcagctt gggctcaggg
     3841 tcgctgcgac gcaatcgtcc ggtccggagc cgggactgtc gggcgtacac aaatcgcccg
     3901 cagaagcgct gccgtctgga ccgatggctg tgtggaagtg ctcgccgata gtggaaacag
     3961 acgccccagc actcgtccta gggcaaagga tctgcagtaa tgaacgcgtt aagtcgacaa
     4021 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     4081 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     4141 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     4201 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     4261 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     4321 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     4381 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
     4441 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     4501 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     4561 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcgtc gactttaaga
     4621 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     4681 gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc
     4741 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     4801 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     4861 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtacgtat
     4921 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag
     4981 agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     5041 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     5101 aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc
     5161 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     5221 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     5281 gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc
     5341 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     5401 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     5461 ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc
     5521 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     5581 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     5641 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     5701 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     5761 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     5821 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     5881 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct
     5941 tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     6001 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     6061 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     6121 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     6181 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     6241 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     6301 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     6361 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     6421 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     6481 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     6541 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     6601 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgaaaact attaactggc
     6661 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     6721 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     6781 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     6841 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     6901 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     6961 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     7021 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     7081 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     7141 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     7201 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     7261 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     7321 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     7381 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     7441 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     7501 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     7561 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     7621 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     7681 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     7741 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     7801 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     7861 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     7921 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     7981 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
     8041 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
     8101 catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa
     8161 gcttaatgta gtcttatgca atactcttgt agtcttgcaa catggtaacg atgagttagc
     8221 aacatgcctt acaaggagag aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt
     8281 acgatcgtgc cttattagga aggcaacaga cgggtctgac atggattgga cgaaccactg
     8341 aattgccgca ttgcagagat attgtattta agtgcctagc tcgatacata aacgggtctc
     8401 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     8461 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     8521 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
     8581 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
     8641 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
     8701 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
     8761 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
     8821 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
     8881 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
     8941 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
     9001 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
     9061 aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga
     9121 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
     9181 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
     9241 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
     9301 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
     9361 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
     9421 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
     9481 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
     9541 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
     9601 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
     9661 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
     9721 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
     9781 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
     9841 cgtttcagac ccacctccca accccgag
//
