LOCUS       sequence-464303-        9709 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses TIMD4 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9709)
  TITLE     Shen Lab plasmids
REFERENCE   2  (bases 1 to 9709)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLAS2w.Phyg-TIMD4
FEATURES             Location/Qualifiers
     source          1..9709
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        295..598
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        599..802
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     753..773
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        847..865
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2051..2074
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    2122..2239
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2288..2798
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2717..2735
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2810..3829
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     "
     misc_feature    3860..4448
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3913..3933)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4331..4342)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4520..4753
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4831..4952
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4868..4887)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4922..4941
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4992..5127
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(5148..5166)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5176..5193)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5176..5192)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5185..5207)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5334..5789
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5421..5440)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5631..5652
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5815..5919
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5920..5988,5989..6780)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6138..6157)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6951..7539
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7440..7459
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7693..7710
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7827..7848
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7863..7868,7869..7886,7887..7893)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7901..7917
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7906..7928
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7925..7941
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7925..7941
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7960..7980
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7962..7980
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8008..8234
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8235..8415
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8462..8587
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9080..9313
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9498..9542
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc
       61 cattcgatta gtgaacggat ctcgacggta tcgatcacga gactagcctc gaggatccac
      121 tagtgaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac
      181 gggccagaaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttctagtta
      241 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
      301 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
      361 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
      421 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
      481 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
      541 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
      601 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
      661 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
      721 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
      781 ggaggtctat ataagcagag ctctctggct aactagagaa cccactgctt actggcttat
      841 cgaaattaat acgactcact atagggagac ccaagctggc tagcagatat ccatcacact
      901 ggcggccgcc accatgtcca aagaacctct cattctctgg ctgatgattg agttttggtg
      961 gctttacctg acaccagtca cttcagagac tgttgtgacg gaggttttgg gtcaccgggt
     1021 gactttgccc tgtctgtact catcctggtc tcacaacagc aacagcatgt gctgggggaa
     1081 agaccagtgc ccctactccg gttgcaagga ggcgctcatc cgcactgatg gaatgagggt
     1141 gacctcaaga aagtcagcaa aatatagact tcaggggact atcccgagag gtgatgtctc
     1201 cttgaccatc ttaaacccca gtgaaagtga cagcggtgtg tactgctgcc gcatagaagt
     1261 gcctggctgg ttcaacgatg taaagataaa cgtgcgcctg aatctacaga gagcctcaac
     1321 aaccacgcac agaacagcaa ccaccaccac acgcagaaca acaacaacaa gccccaccac
     1381 cacccgacaa atgacaacaa ccccagctgc acttccaaca acagtcgtga ccacacccga
     1441 tctcacaacc ggaacaccac tccagatgac aaccattgcc gtcttcacaa cagcaaacac
     1501 gtgcctttca ctaaccccaa gcacccttcc ggaggaagcc acaggtcttc tgactcccga
     1561 gccttctaag gaagggccca tcctcactgc agaatcagaa actgtcctcc ccagtgattc
     1621 ctggagtagt gctgagtcta cttctgctga cactgtcctg ctgacatcca aagagtccaa
     1681 agtttgggat ctcccatcaa catcccacgt gtcaatgtgg aaaacgagtg attctgtgtc
     1741 ttctcctcag cctggagcgt ctgatacagc agttcctgag cagaacaaaa caacaaaaac
     1801 aggacagatg gatggaatac ccatgtcaat gaagaatgaa atgcccatct cccaactact
     1861 gatgatcatc gccccctcct tgggatttgt gctcttcgca ttgtttgtgg cgtttctcct
     1921 gagagggaaa ctcatggaaa cctattgttc gcagaaacac acaaggctag actacattgg
     1981 agatagtaaa aatgtcctca atgacgtgca gcatggaagg gaagacgaag acggcctttt
     2041 taccctcgag gattacaagg atgacgacga taagtaggcc gaattctcga cctcgagaca
     2101 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
     2161 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     2221 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccac tttggccgcg
     2281 gctcgagggg gttggggttg cgccttttcc aaggcagccc tgggtttgcg cagggacgcg
     2341 gctgctctgg gcgtggttcc gggaaacgca gcggcgccga ccctgggtct cgcacattct
     2401 tcacgtccgt tcgcagcgtc acccggatct tcgccgctac ccttgtgggc cccccggcga
     2461 cgcttcctgc tccgccccta agtcgggaag gttccttgcg gttcgcggcg tgccggacgt
     2521 gacaaacgga agccgcacgt ctcactagta ccctcgcaga cggacagcgc cagggagcaa
     2581 tggcagcgcg ccgaccgcga tgggctgtgg ccaatagcgg ctgctcagca gggcgcgccg
     2641 agagcagcgg ccgggaaggg gcggtgcggg aggcggggtg tggggcggta gtgtgggccc
     2701 tgttcctgcc cgcgcggtgt tccgcattct gcaagcctcc ggagcgcacg tcggcagtcg
     2761 gctccctcgt tgaccgaatc accgacctct ctccccaggg ggatccacca tgaaaaagcc
     2821 tgaactcacc gctacctctg tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga
     2881 cctgatgcag ctctccgagg gcgaagaatc tcgggctttc agcttcgatg tgggagggcg
     2941 tggatatgtc ctgcgggtga atagctgcgc cgatggtttc tacaaagatc gctatgttta
     3001 tcggcacttt gcatccgccg ctctccctat tcccgaagtg cttgacattg gggagttcag
     3061 cgagagcctg acctattgca tctcccgccg tgcacagggt gtcaccttgc aagacctgcc
     3121 tgaaaccgaa ctgcccgctg ttctccagcc cgtcgccgag gccatggatg ccatcgctgc
     3181 cgccgatctt agccagacca gcgggttcgg cccattcgga cctcaaggaa tcggtcaata
     3241 cactacatgg cgcgatttca tctgcgctat tgctgatccc catgtgtatc actggcaaac
     3301 tgtgatggac gacaccgtca gtgcctccgt cgcccaggct ctcgatgagc tgatgctttg
     3361 ggccgaggac tgccccgaag tccggcacct cgtgcacgcc gatttcggct ccaacaatgt
     3421 cctgaccgac aatggccgca taacagccgt cattgactgg agcgaggcca tgttcgggga
     3481 ttcccaatac gaggtcgcca acatcttctt ctggaggccc tggttggctt gtatggagca
     3541 gcagacccgc tacttcgagc ggaggcatcc cgagcttgca ggatctcctc ggctccgggc
     3601 ttatatgctc cgcattggtc ttgaccaact ctatcagagc ttggttgacg gcaatttcga
     3661 tgatgcagct tgggctcagg gtcgctgcga cgcaatcgtc cggtccggag ccgggactgt
     3721 cgggcgtaca caaatcgccc gcagaagcgc tgccgtctgg accgatggct gtgtggaagt
     3781 gctcgccgat agtggaaaca gacgccccag cactcgtcct agggcaaagg atctgcagta
     3841 atgaacgcgt taagtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg
     3901 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     3961 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     4021 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     4081 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     4141 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     4201 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
     4261 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     4321 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     4381 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     4441 ctccccgcgt cgactttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     4501 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt
     4561 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     4621 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     4681 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     4741 aaaatctcta gcagtacgta tagtagttca tgtcatctta ttattcagta tttataactt
     4801 gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac
     4861 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     4921 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc
     4981 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     5041 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     5101 agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc
     5161 gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     5221 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     5281 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc
     5341 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     5401 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     5461 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     5521 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     5581 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     5641 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     5701 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     5761 ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga
     5821 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     5881 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     5941 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     6001 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     6061 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     6121 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     6181 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     6241 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     6301 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     6361 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     6421 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     6481 ttgcgaaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     6541 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     6601 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     6661 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     6721 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     6781 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     6841 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     6901 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     6961 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     7021 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     7081 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     7141 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     7201 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     7261 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     7321 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     7381 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     7441 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     7501 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     7561 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     7621 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     7681 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     7741 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     7801 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     7861 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
     7921 cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg
     7981 gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg tagtcttgca
     8041 acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc
     8101 gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga
     8161 catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt aagtgcctag
     8221 ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag ctctctggct
     8281 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     8341 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     8401 ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga
     8461 gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg
     8521 actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga
     8581 gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc
     8641 agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg
     8701 attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca
     8761 gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc
     8821 aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa
     8881 gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca
     8941 gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag
     9001 taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag
     9061 aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca
     9121 ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag
     9181 tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca
     9241 cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg
     9301 atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc
     9361 cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga
     9421 tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat
     9481 cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt
     9541 tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag
     9601 taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta
     9661 ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgag
//
