LOCUS       sequence-464361-       15471 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GATEWAY compatible vector with a dexamethasone (DEX)-inducible 
            promoter driving in frame epitope fusion of 3xHA tag paired with 
            ccdb selection toxin and RUBY marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15471)
  TITLE     GATEWAY compatible vector with a promoter driving in frame epitope 
            fusion paired with ccdb selection toxin and FP marker
REFERENCE   2  (bases 1 to 15471)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: DEXsynth:3xhA-ccdb + RUBY
FEATURES             Location/Qualifiers
     source          1..15471
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             718..1023
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     902..921
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1064..1188)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1985..5935
                     /label=RUBY
     CDS             3485..3541
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4376..4432
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     terminator      5950..6202
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    6257..6281
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(6404..6992)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6484..6503)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7384..8574)
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     8203..8222
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        complement(join(8622..8627,8628..8644,8645..8650))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     rep_origin      8943..9137
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9203..10276)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10705..11334)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    11598..11622
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             12874..13107
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     promoter        14718..14764
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     CDS             14832..14921
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     protein_bind    14943..15067
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(15104..15109,15110..15127,15128..15134)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             join(15188..15471,1..376)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(15254..15273)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa taccacgacg atttccggca
       61 gtttctacac atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc
      121 taaagggttt attgagaata tgtttttcgt ctcagccaat ccctgggtga gtttcaccag
      181 ttttgattta aacgtggcca atatggacaa cttcttcgcc cccgttttca ccatgggcaa
      241 atattatacg caaggcgaca aggtgctgat gccgctggcg attcaggttc atcatgccgt
      301 ttgtgatggc ttccatgtcg gcagaatgct taatgaatta caacagtact gcgatgagtg
      361 gcagggcggg gcgtaaacgc gtggatccgg cttactaaaa gccagataac agtatgcgta
      421 tttgcgcgct gatttttgcg gtataagaat atatactgat atgtataccc gaagtatgtc
      481 aaaaagaggt atgctatgaa gcagcgtatt acagtgacag ttgacagcga cagctatcag
      541 ttgctcaagg catatatgat gtcaatatct ccggtctggt aagcacaacc atgcagaatg
      601 aagcccgtcg tctgcgtgcc gaacgctgga aagcggaaaa tcaggaaggg atggctgagg
      661 tcgcccggtt tattgaaatg aacggctctt ttgctgacga gaacaggggc tggtgaaatg
      721 cagtttaagg tttacaccta taaaagagag agccgttatc gtctgtttgt ggatgtacag
      781 agtgatatta ttgacacgcc cgggcgacgg atggtgatcc ccctggccag tgcacgtctg
      841 ctgtcagata aagtctcccg tgaactttac ccggtggtgc atatcgggga tgaaagctgg
      901 cgcatgatga ccaccgatat ggccagtgtg ccggtctccg ttatcgggga agaagtggct
      961 gatctcagcc accgcgaaaa tgacatcaaa aacgccatta acctgatgtt ctggggaata
     1021 taaatgtcag gctcccttat acacagccag tctgcaggtc gaccatagtg actggatatg
     1081 ttgtgtttta cagtattatg tagtctgttt tttatgcaaa atctaattta atatattgat
     1141 atttatatca ttttacgttt ctcgttcagc tttcttgtac aaagtggtcc taggttcggt
     1201 tcgagtatta tggcattggg aaaactgttt ttcttgtacc atttgttgtg cttgtaattt
     1261 actgtgtttt ttattcggtt ttcgctatcg aactgtgaaa tggaaatgga tggagaagag
     1321 ttaatgaatg atatggtcct tttgttcatt ctcaaattaa tattatttgt tttttctctt
     1381 atttgttgtg tgttgaattt gaaattataa gagatatgca aacattttgt tttgagtaaa
     1441 aatgtgtcaa atcaaggttc cagaaggtaa ttatccaaga tgtagcatca agaatccaat
     1501 gtttacggga aaaactatgg aagtattatg tgagctcagc aagaagcaga tcaatatgcg
     1561 gcacatatgc aacctatgtt caaaaatgaa gaatgtacag atacaagatc ctatactgcc
     1621 agaatacgaa gaagaatacg tagaaattga aaaagaagaa ccaggcgaag aaaagaatct
     1681 tgaagacgta agcactgacg acaacaatga aaagaagaag ataaggtcgg tgattgtgaa
     1741 agagacatag aggacacatg taaggtggaa aatgtaaggg cggaaagtaa ccttatcaca
     1801 aaggaatctt atcccccact acttatcctt ttatattttt ccgtgtcatt tttgcccttg
     1861 agttttccta tataaggaac caagttcggc atttgtgaaa acaagaaaaa atttggtgta
     1921 agctattttc tttgaagtac tgaggataca acttcagaga aatttgtaag tttgtgatcc
     1981 gcaaatggat catgcgaccc tcgccatgat cctcgcgatc tggttcatca gcttccactt
     2041 catcaagctg ctgttctccc agcagaccac caagctgctt ccgccaggac caaagccgct
     2101 tccgatcatc ggcaacatcc ttgaggtggg caagaagccg catcggtcct tcgccaacct
     2161 cgccaagatt cacggcccac tcatttccct cagactcggc tctgtgacca ccatcgttgt
     2221 gtcctctgcc gacgtggcca aagagatgtt cctcaagaag gatcacccgc tctccaaccg
     2281 cacgatcccg aatagtgtta cagccggcga ccaccacaag ctcaccatgt cttggctccc
     2341 ggtgtctccg aagtggcgca acttccgcaa gattaccgcc gtgcatctgc tctccccaca
     2401 gagactcgat gcctgccaga cattcaggca cgccaaggtg cagcagctct acgagtacgt
     2461 tcaagagtgc gcccagaaag gccaggccgt ggatattggc aaggccgcct ttacgaccag
     2521 cctcaacctc ctcagcaagc tgttcttcag cgtcgagctg gcgcaccaca agtcccatac
     2581 cagccaagag ttcaaagagc tgatctggaa catcatggaa gatataggca agccgaacta
     2641 cgccgactac ttcccgattc tcggctgcgt tgacccatct ggcattagaa gaaggctcgc
     2701 ctgctccttc gacaagctga tcgccgtgtt ccagggcatc atctgcgaga gactcgcccc
     2761 agattcctcc accacaacta ccaccaccac cgacgacgtg ctcgatgtgc tcctccagct
     2821 gttcaagcag aacgagctga cgatgggcga gatcaaccac ctcctcgtgg acatcttcga
     2881 cgccggcacc gataccacat cctccacatt cgagtgggtg atgaccgagc tgatccgcaa
     2941 tccagagatg atggaaaagg cccaagagga aatcaagcag gtcctcggca aggacaagca
     3001 gatccaagag tccgacatca tcaacctgcc gtacctccag gcgatcatca aagagacact
     3061 ccgcctccat ccgccgaccg tgttcttgct cccaagaaag gccgacaccg atgtcgagct
     3121 gtacggctac atcgtgccga aggatgccca gatcctcgtg aacctctggg ccattggcag
     3181 ggacccaaac gcctggcaga acgccgatat tttcagccca gagcgcttca tcggctgcga
     3241 gatcgatgtt aagggccgcg atttcggcct ccttccattt ggcgctggcc gcagaatttg
     3301 cccaggcatg aatctcgcca tcaggatgct caccctcatg ctcgccacac tcctccagtt
     3361 cttcaactgg aagctcgaag gcgacatctc cccgaaggac ctcgacatgg acgagaagtt
     3421 cggcattgcg ctccaaaaga ccaagccgct caagctcatc ccgattccgc gctacggtag
     3481 cggagctacc aattttagcc tccttaagca ggcaggtgat gtagaagaga accccgggcc
     3541 tatgaagatg atgaacggcg aggacgccaa cgaccagatg atcaaagagt ccttcttcat
     3601 cacccacggc aacccgatcc tcaccgtcga ggatacacat ccgctcaggc cgttcttcga
     3661 gacatggcgc gagaagattt tctccaagaa gccgaaggcc atcctcatca tctccggcca
     3721 ctgggagaca gtgaagccaa ccgtgaacgc cgtgcacatc aacgacacca tccacgactt
     3781 cgacgactac ccagccgcca tgtaccagtt caagtaccca gctccaggcg agccagagct
     3841 tgcgagaaag gtggaagaga tcctcaagaa gtccgggttc gagacagccg agacagacca
     3901 aaagaggggc cttgatcacg gcgcctgggt tccactcatg ctcatgtatc cagaggcgga
     3961 catcccggtg tgccagctct cagttcagcc acatctcgac ggcacctacc actacaatct
     4021 cggcagagcc ctcgcgccgc tcaagaatga tggcgtgctc attattggct ccggcagcgc
     4081 cacacatcca ctcgatgaga caccgcacta cttcgatggt gttgcccctt gggccgctgc
     4141 cttcgattct tggcttagga aggccctcat caacggccgc ttcgaggaag tgaacatcta
     4201 cgagagcaag gccccgaact ggaagctcgc ccatccattt ccagagcact tctacccgct
     4261 ccacgttgtg ctcggcgctg ctggtgaaaa gtggaaggcc gagctgatcc actcctcctg
     4321 ggatcatggc acactttgcc acggctccta caagttcacc tccgccggat ccggagcaac
     4381 caactttagc ctgctcaagc aagcaggaga tgttgaggaa aatcctggcc ccatgaccgc
     4441 catcaagatg aacaccaacg gcgagggcga gacacagcac atcctcatga tcccgttcat
     4501 ggcgcagggc cacctcaggc catttctcga actcgccatg ttcctctaca agcgctccca
     4561 cgtgatcatc accctgctca caactccgct caatgccggc ttcctcaggc acctccttca
     4621 ccaccattcc tactcctcca gcggcatcag gatcgtcgag ctgccattca actccaccaa
     4681 ccacggactc ccaccgggca tcgagaacac cgataagctc acactcccgc tcgtggtgtc
     4741 cctcttccat tccaccatca gcctcgatcc gcacctccgc gattacatct ccaggcattt
     4801 cagcccagcc aggccaccac tctgcgtgat ccatgatgtg ttcctcggct gggttgacca
     4861 ggtggccaag gatgtgggct ctacaggcgt ggtgttcaca acaggcggcg cttatggcac
     4921 atccgcctac gtgtccatct ggaacgatct cccgcaccag aactactccg acgaccaaga
     4981 gttcccgctg ccaggcttcc cagagaacca taagttccgc aggtcccagc tccatcggtt
     5041 cctcagatat gccgacggct ccgacgattg gtccaagtat ttccagccgc agctccgcca
     5101 gtccatgaag tcttttggct ggctctgcaa ctccgtggaa gagatcgaga cactcggctt
     5161 ctccatcctc cgcaactaca ccaagctgcc gatctggggc atcggcccac ttattgcttc
     5221 cccagtgcag cactcctcct ccgacaacaa ttcaacaggc gccgagttcg tgcagtggct
     5281 cagcctcaaa gagccggact ccgtcctcta catctccttc ggctcccaga acacgatcag
     5341 cccgacgcag atgatggaac tcgctgctgg ccttgagtcc tccgagaagc cattcctctg
     5401 ggtgatcaga gccccgttcg gcttcgacat caacgaagag atgcgcccag agtggctgcc
     5461 agagggcttt gaggaacgca tgaaggtgaa gaaacagggc aagctcgtgt acaagctcgg
     5521 cccgcagctt gagatcctca accatgaatc catcggcggc tttctcaccc actgcggatg
     5581 gaacagcatc cttgagtctc ttcgcgaggg cgttccgatg cttggatggc cacttgctgc
     5641 cgagcaggcc tacaacctca agtacctcga agatgagatg ggcgtcgcgg ttgagcttgc
     5701 tagaggcctc gaaggcgaga tctccaaaga gaaggtcaag cgcatcgtcg agatgatcct
     5761 tgagcgcaac gagggctcca aaggctggga gatgaagaat cgcgccgtgg aaatgggcaa
     5821 aaagctcaag gacgccgtga acgaggaaaa agagctgaag ggctcctccg tgaaggcgat
     5881 cgacgatttc ctcgacgccg tcatgcaggc caaacttgag ccaagcctcc agtgatagtg
     5941 agatgtgcag atcgttcaaa catttggcaa taaagtttct taagattgaa tcctgttgcc
     6001 ggtcttgcga tgattatcat ataatttctg ttgaattacg ttaagcatgt aataattaac
     6061 atgtaatgca tgacgttatt tatgagatgg gtttttatga ttagagtccc gcaattatac
     6121 atttaatacg cgatagaaaa caaaatatag cgcgcaaact aggataaatt atcgcgcgcg
     6181 gtgtcatcta tgttactaga tccgatgata agctgtcaaa catgagggac acgaagtggt
     6241 ttaaactatc agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt
     6301 attagaataa tcggatattt aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat
     6361 gtgccaacca caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     6421 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     6481 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     6541 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     6601 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     6661 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     6721 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     6781 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     6841 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     6901 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     6961 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     7021 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     7081 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     7141 atgagtaaac ttggtctgac agctaaaaca attcatccag taaaatataa tcgaatatta
     7201 tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga
     7261 tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca
     7321 agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc
     7381 cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag
     7441 gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata
     7501 aatcgccgtg acgatcagcg gtccaatgat cgaagttagg ctggtaagag ccgcgagcga
     7561 tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc
     7621 gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg
     7681 cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc
     7741 ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg
     7801 caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc
     7861 ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa
     7921 gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg
     7981 gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa
     8041 gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa
     8101 ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca
     8161 agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata
     8221 ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag
     8281 gatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga
     8341 agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca
     8401 tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc
     8461 ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac
     8521 agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg
     8581 tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat
     8641 aagatgtcaa acatgagaat tcttgaaaat attgcgctgc catttttggg gtgaggccgt
     8701 tcgcggccga ggggcgcagc ccctgggggg atgggagctc ggtgccttga tgtgggcgcc
     8761 ggcggtcgag tggcgacggc gcggcttgtc cgcgccctgg tagattgcct ggccgtaggc
     8821 cagccatttt tgagcggcca gcggccgcga taggccgacg cgaagcggcg gggcgtaggg
     8881 agcgcagcga ccgaagggta ggcgcttttt gcagctcttc ggctgtgcgc tggccagaca
     8941 gttatgcaca ggccaggcgg gttttaagag ttttaataag ttttaaagag ttttaggcgg
     9001 aaaaatcgcc ttttttctct tttatatcag tcacttacat gtgtgaccgg ttcccaatgt
     9061 acggctttgg gttcccaatg tacgggttcc ggttcccaat gtacggcttt gggttcccaa
     9121 tgtacgtgct atccacagga aagaagcttt ttcgaccttt ttcccctgct agggcaattt
     9181 gccctagcat ctgctccgta cattaggaac cggcggatgc ttcgccctcg atcaggttgc
     9241 ggtagcgcat gactaggatc gggccagcct gccccgcctc ctccttcaaa tcgtactccg
     9301 gcaggtcatt tgacccgatc agcttgcgca cggtgaaaca gaacttcttg aactctccgg
     9361 cgctgccact gcgttcgtag atcgtcttga acaaccatct ggcttctgcc ttgcctgcgg
     9421 cgcggcgtgc caggcggtag agaaaacggc cgatgccggg atcgatcaaa aagtaatcgg
     9481 ggtgaaccgt cagcacgtcc gggttcttgc cttctgtgat ctcgcggtac atccaatcag
     9541 ctagctcgat ctcgatgtac tccggccgcc cggtttcgct ctttacgatc ttgtagcggc
     9601 taatcaaggc ttcaccctcg gataccgtca ccaggcggcc gttcttggcc ttcttcgtac
     9661 gctgcatggc aacgtgcgtg gtgtttaacc gaatgcaggt ttctaccagg tcgtctttct
     9721 gctttccgcc atcggctcgc cggcagaact tgagtacgtc cgcaacgtgt ggacggaaca
     9781 cgcggccggg cttgtctccc ttcccttccc ggtatcggtt catggattcg gttagatggg
     9841 aaaccgccat cagtaccagg tcgtaatccc acacactggc catgccggcc ggccctgcgg
     9901 aaacctctac gtgcccgtct ggaagctcgt agcggatcac ctcgccagct cgtcggtcac
     9961 gcttcgacag acggaaaacg gccacgtcca tgatgctgcg actatcgcgg gtgcccacgt
    10021 catagagcat cggaacgaaa aaatctggtt gctcgtcgcc cttgggcggc ttcctaatcg
    10081 acgacgcacc ggctgccggc ggttgccggg attctttgcg gattcgatca gcggccgctt
    10141 gccacgattc accggggcgt gcttctgcct cgatgcgttg ccgctgggcg gcctgcgcgg
    10201 ccttcaactt ctccaccagg tcatcaccca gcgccgcgcc gatttgtacc gggccggatg
    10261 gtttgcgacc gctcacgccg attcctcggg cttgggggtt ccagtgccat tgcagggccg
    10321 gcagacaacc cagccgctta cgcctggcca accgcccgtt cctccacaca tggggcattc
    10381 cacggcgtcg gtgcctggtt gttcttgatt ttccatgccg cctcctttag ccgctaaaat
    10441 tcatctactc atttattcat ttgctcattt actctggtag ctgcgcgatg tattcagata
    10501 gcagctcggt aatggtcttg ccttggcgta ccgcgtacat cttcagcttg gtgtgatcct
    10561 ccgccggcaa ctgaaagttg acccgcttca tggctggcgt gtctgccagg ctggccaacg
    10621 ttgcagcctt gctgctgcgt gcgctcggac ggccggcact tagcgtgttt gtgcttttgc
    10681 tcattttctc tttacctcat taactcaaat gagttttgat ttaatttcag cggccagcgc
    10741 ctggacctcg cgggcagcgt cgccctcggg ttctgattca agaacggttg tgccggcggc
    10801 ggcagtgcct gggtagctca cgcgctgcgt gatacgggac tcaagaatgg gcagctcgta
    10861 cccggccagc gcctcggcaa cctcaccgcc gatgcgcgtg cctttgatcg cccgcgacac
    10921 gacaaaggcc gcttgtagcc ttccatccgt gacctcaatg cgctgcttaa ccagctccac
    10981 caggtcggcg gtggcccata tgtcgtaagg gcttggctgc accggaatca gcacgaagtc
    11041 ggctgccttg atcgcggaca cagccaagtc cgccgcctgg ggcgctccgt cgatcactac
    11101 gaagtcgcgc cggccgatgg ccttcacgtc gcggtcaatc gtcgggcggt cgatgccgac
    11161 aacggttagc ggttgatctt cccgcacggc cgcccaatcg cgggcactgc cctggggatc
    11221 ggaatcgact aacagaacat cggccccggc gagttgcagg gcgcgggcta gatgggttgc
    11281 gatggtcgtc ttgcctgacc cgcctttctg gttaagtaca gcgataacct tcatgcgttc
    11341 cccttgcgta tttgtttatt tactcatcgc atcatatacg cagcgaccgc atgacgcaag
    11401 ctgttttact caaatacaca tcaccttttt agacggcggc gctcggtttc ttcagcggcc
    11461 aagctggccg gccaggccgc cagcttggca tcagacaaac cggccaggat ttcatgcagc
    11521 cgcacggctg atgggctgcc tgtatcgagt ggtgattttg tgccgagctg ccggtcgggg
    11581 agctgttggc tggctggtgg caggatatat tgtggtgtaa acaaattgac gcttagacaa
    11641 cttaataaca cattgcggac gtttttaatg tactggggtg gatgcagtgg gccccactct
    11701 gtggaagaca atgcctccgg agtaacagat aaagcgaagc ttgaaggtgc atgtgactcc
    11761 gtcaagatta cgaaaccgcc aactaccacg caaattgcaa ttctcaattt cctagaagga
    11821 ctctccgaaa atgcatccaa taccaaatat tacccgtgtc ataggcacca agtgacacca
    11881 tacatgaaca cgcgtcacaa tatgactgga gaagggttcc acaccttatg ctataaaacg
    11941 ccccacaccc ctcctccttc cttcgcagtt caattccaat atattccatt ctctctgtgt
    12001 atttccctac ctctcccttc aaggttagtc gatttcttct gtttttcttc ttcgttcttt
    12061 ccatgaattg tgtatgttct ttgatcaata cgatgttgat ttgattgtgt tttgtttggt
    12121 ttcatcgatc ttcaattttc ataatcagat tcagctttta ttatctttac aacaacgtcc
    12181 ttaatttgat gattctttaa tcgtagattt gctctaatta gagctttttc atgtcagatc
    12241 cctttacaac aagccttaat tgttgattca ttaatcgtag attagggctt ttttcattga
    12301 ttacttcaga tccgttaaac gtaaccatag atcagggctt tttcatgaat tacttcagat
    12361 ccgttaaaca acagccttat tttttatact tctgtggttt ttcaagaaat tgttcagatc
    12421 cgttgacaaa aagccttatt cgttgattct atatcgtttt tcgagagata ttgctcagat
    12481 ctgttagcaa ctgccttgtt tgttgattct attgccgtgg attagggttt tttttcacga
    12541 gattgcttca gatccgtact taagattacg taatggattt tgattctgat ttatctgtga
    12601 ttgttgactc gacagcggcc gcaccggcgc gccatggcag atccaatgaa gctactgtct
    12661 tctatcgaac aagcatgcga tatttgccga cttaaaaagc tcaagtgctc caaagaaaaa
    12721 ccgaagtgcg ccaagtgtct gaagaacaac tgggagtgtc gctactctcc caaaaccaaa
    12781 agatctccgc tgactagggc acatctgaca gaagtggaat caaggctaga aagactggaa
    12841 cagctatttc tactgatttt tccaaggtcg agcgcccccc cgaccgatgt cagcctgggg
    12901 gacgagctcc acttagacgg cgaggacgtg gcgatggcgc atgccgacgc gctagacgat
    12961 ttcgatctgg acatgttggg ggacggggat tccccgggtc cgggatttac cccccacgac
    13021 tccgccccct acggcgctct ggatatggcc gacttcgagt ttgagcagat gtttaccgat
    13081 gcccttggaa ttgacgagta cggtggggat ccaattcagc aagccactgc aggagtctca
    13141 caagacactt cggaaaatcc taacaaaaca atagttcctg ctgcattacc acagctcacc
    13201 cctaccttgg tgtcactgct ggaggtgatt gaacccgagg tgttgtatgc aggatatgat
    13261 agctctgttc cagattcagc atggagaatt atgaccacac tcaacatgtt aggtgggcgt
    13321 caagtgattg cagcagtgaa atgggcaaag gcgataccag gcttcagaaa cttacacctg
    13381 gatgaccaaa tgaccctgct acagtactca tggatgtttc tcatggcatt tgccctgggt
    13441 tggagatcat acagacaatc aagtggaaac ctgctctgct ttgctcctga tctgattatt
    13501 aatgagcaga gaatgtctct accctgcatg tatgaccaat gtaaacacat gctgtttgtc
    13561 tcctctgaat tacaaagatt gcaggtatcc tatgaagagt atctctgtat gaaaacctta
    13621 ctgcttctct cctcagttcc taaggaaggt ctgaagagcc aagagttatt tgatgagatt
    13681 cgaatgactt atatcaaaga gctaggaaaa gccatcgtca aaagggaagg gaactccagt
    13741 cagaactggc aacggtttta ccaactgaca aagcttctgg actccatgca tgaggtggtt
    13801 gagaatctcc ttacctactg cttccagaca tttttggata agaccatgag tattgaattc
    13861 ccagagatgt tagctgaaat catcactaat cagataccaa aatattcaaa tggaaatatc
    13921 aaaaagcttc tgtttcatca aaaatgactc gacctaactg agtaagctag cttgttcgag
    13981 tattatggca ttgggaaaac tgtttttctt gtaccatttg ttgtgcttgt aatttactgt
    14041 gttttttatt cggttttcgc tatcgaactg tgaaatggaa atggatggag aagagttaat
    14101 gaatgatatg gtccttttgt tcattctcaa attaatatta tttgtttttt ctcttatttg
    14161 ttgtgtgttg aatttgaaat tataagagat atgcaaacat tttgttttga gtaaaaatgt
    14221 gtcaaatcgt ggcctctaat gaccgaagtt aatatgagga gtaaaacact agatccccaa
    14281 acaagcttga aactgaaggc gggaaacgac aatctgatca tgagcggaga attaagggag
    14341 tcacgttatg acccccgccg atgacgcggg acaagccgtt ttacgtttgg aactgacata
    14401 tgaaaatatt ttttaatgca ttttatgact tgccaattga ttgacaacat gcatcaatcc
    14461 gcggttatga ctctcttaag agagtcatga attcgagcgg gaattgatcc cccctcgaca
    14521 gcttgcatgc cggtcgactc tagaggatcc gggtgacagc cctccgacgg gtgacagccc
    14581 tccgacgggt gacagccctc cgaattctag aggatccggg tgacagccct ccgacgggtg
    14641 acagccctcc gacgggtgac agccctccga attcgagctc agtacccggg gatctgtcga
    14701 cctcgatcga gatcttcgca agacccttcc tctatataag gaagttcatt tcatttggag
    14761 aggacacgct gaagctagtc gactctagcc tcgaatcctt aggtaccatg agcgggttaa
    14821 ttaacatctt ttacccatac gatgttcctg actatgcggg ctatccctat gacgtcccgg
    14881 actatgcagg atcctatcca tatgacgttc cagattacgc tgctcagtgc agcgctgtta
    14941 tcacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa tgatataaat atcaatatat
    15001 taaattagat tttgcataaa aaacagacta cataatactg taaaacacaa catatccagt
    15061 cactatggcg gcccctcgag gggccgcatt aggcacccca ggctttacac tttatgcttc
    15121 cggctcgtat aatgtgtgga ttttgagtta ggatccgtcg agattttcag gagctaagga
    15181 agctaaaatg gagaaaaaaa tcactggata taccaccgtt gatatatccc aatggcatcg
    15241 taaagaacat tttgaggcat ttcagtcagt tgctcaatgt acctataacc agaccgttca
    15301 gctggatatt acggcctttt taaagaccgt aaagaaaaat aagcacaagt tttatccggc
    15361 ctttattcac attcttgccc gcctgatgaa tgctcatccg gaattccgta tggcaatgaa
    15421 agacggtgag ctggtgatat gggatagtgt tcacccttgt tacaccgttt t
//
