LOCUS       sequence-464362-       12084 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GATEWAY compatible vector with a dexamethasone (DEX)-inducible 
            promoter driving in frame epitope fusion of ccdb selection toxin and
            mCherry marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12084)
  TITLE     GATEWAY compatible vector with a promoter driving in frame epitope 
            fusion paired with ccdb selection toxin and FP marker
REFERENCE   2  (bases 1 to 12084)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: DEXsynth:ccdb+ mcherry
FEATURES             Location/Qualifiers
     source          1..12084
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     485..504
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        complement(join(904..909,910..926,927..932))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     rep_origin      1225..1419
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(1485..2558)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(2987..3616)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    3880..3904
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5156..5389
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     promoter        7000..7046
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     protein_bind    7090..7214
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(7239..7244,7245..7262,7263..7269)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             7323..7982
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(7389..7408)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             8324..8629
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     8508..8527
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(8670..8794)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             9597..10307
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     10010..10029
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     terminator      10316..10568
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    10623..10647
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(10770..11358)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10850..10869)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11750..12084,1..856))
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
ORIGIN
        1 ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga
       61 cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg
      121 tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc
      181 ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg
      241 cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct
      301 tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg
      361 ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg
      421 ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc
      481 catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg
      541 ccacgatgcg tccggcgtag aggatccaca ggacgggtgt ggtcgccatg atcgcgtagt
      601 cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca
      661 gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc
      721 catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg
      781 gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg
      841 cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac
      901 cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaaa atattgcgct
      961 gccatttttg gggtgaggcc gttcgcggcc gaggggcgca gcccctgggg ggatgggagc
     1021 tcggtgcctt gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg tccgcgccct
     1081 ggtagattgc ctggccgtag gccagccatt tttgagcggc cagcggccgc gataggccga
     1141 cgcgaagcgg cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt ttgcagctct
     1201 tcggctgtgc gctggccaga cagttatgca caggccaggc gggttttaag agttttaata
     1261 agttttaaag agttttaggc ggaaaaatcg ccttttttct cttttatatc agtcacttac
     1321 atgtgtgacc ggttcccaat gtacggcttt gggttcccaa tgtacgggtt ccggttccca
     1381 atgtacggct ttgggttccc aatgtacgtg ctatccacag gaaagaagct ttttcgacct
     1441 ttttcccctg ctagggcaat ttgccctagc atctgctccg tacattagga accggcggat
     1501 gcttcgccct cgatcaggtt gcggtagcgc atgactagga tcgggccagc ctgccccgcc
     1561 tcctccttca aatcgtactc cggcaggtca tttgacccga tcagcttgcg cacggtgaaa
     1621 cagaacttct tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt gaacaaccat
     1681 ctggcttctg ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg gccgatgccg
     1741 ggatcgatca aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt gccttctgtg
     1801 atctcgcggt acatccaatc agctagctcg atctcgatgt actccggccg cccggtttcg
     1861 ctctttacga tcttgtagcg gctaatcaag gcttcaccct cggataccgt caccaggcgg
     1921 ccgttcttgg ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa ccgaatgcag
     1981 gtttctacca ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa cttgagtacg
     2041 tccgcaacgt gtggacggaa cacgcggccg ggcttgtctc ccttcccttc ccggtatcgg
     2101 ttcatggatt cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc ccacacactg
     2161 gccatgccgg ccggccctgc ggaaacctct acgtgcccgt ctggaagctc gtagcggatc
     2221 acctcgccag ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc catgatgctg
     2281 cgactatcgc gggtgcccac gtcatagagc atcggaacga aaaaatctgg ttgctcgtcg
     2341 cccttgggcg gcttcctaat cgacgacgca ccggctgccg gcggttgccg ggattctttg
     2401 cggattcgat cagcggccgc ttgccacgat tcaccggggc gtgcttctgc ctcgatgcgt
     2461 tgccgctggg cggcctgcgc ggccttcaac ttctccacca ggtcatcacc cagcgccgcg
     2521 ccgatttgta ccgggccgga tggtttgcga ccgctcacgc cgattcctcg ggcttggggg
     2581 ttccagtgcc attgcagggc cggcagacaa cccagccgct tacgcctggc caaccgcccg
     2641 ttcctccaca catggggcat tccacggcgt cggtgcctgg ttgttcttga ttttccatgc
     2701 cgcctccttt agccgctaaa attcatctac tcatttattc atttgctcat ttactctggt
     2761 agctgcgcga tgtattcaga tagcagctcg gtaatggtct tgccttggcg taccgcgtac
     2821 atcttcagct tggtgtgatc ctccgccggc aactgaaagt tgacccgctt catggctggc
     2881 gtgtctgcca ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg acggccggca
     2941 cttagcgtgt ttgtgctttt gctcattttc tctttacctc attaactcaa atgagttttg
     3001 atttaatttc agcggccagc gcctggacct cgcgggcagc gtcgccctcg ggttctgatt
     3061 caagaacggt tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc gtgatacggg
     3121 actcaagaat gggcagctcg tacccggcca gcgcctcggc aacctcaccg ccgatgcgcg
     3181 tgcctttgat cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc gtgacctcaa
     3241 tgcgctgctt aaccagctcc accaggtcgg cggtggccca tatgtcgtaa gggcttggct
     3301 gcaccggaat cagcacgaag tcggctgcct tgatcgcgga cacagccaag tccgccgcct
     3361 ggggcgctcc gtcgatcact acgaagtcgc gccggccgat ggccttcacg tcgcggtcaa
     3421 tcgtcgggcg gtcgatgccg acaacggtta gcggttgatc ttcccgcacg gccgcccaat
     3481 cgcgggcact gccctgggga tcggaatcga ctaacagaac atcggccccg gcgagttgca
     3541 gggcgcgggc tagatgggtt gcgatggtcg tcttgcctga cccgcctttc tggttaagta
     3601 cagcgataac cttcatgcgt tccccttgcg tatttgttta tttactcatc gcatcatata
     3661 cgcagcgacc gcatgacgca agctgtttta ctcaaataca catcaccttt ttagacggcg
     3721 gcgctcggtt tcttcagcgg ccaagctggc cggccaggcc gccagcttgg catcagacaa
     3781 accggccagg atttcatgca gccgcacggc tgatgggctg cctgtatcga gtggtgattt
     3841 tgtgccgagc tgccggtcgg ggagctgttg gctggctggt ggcaggatat attgtggtgt
     3901 aaacaaattg acgcttagac aacttaataa cacattgcgg acgtttttaa tgtactgggg
     3961 tggatgcagt gggccccact ctgtggaaga caatgcctcc ggagtaacag ataaagcgaa
     4021 gcttgaaggt gcatgtgact ccgtcaagat tacgaaaccg ccaactacca cgcaaattgc
     4081 aattctcaat ttcctagaag gactctccga aaatgcatcc aataccaaat attacccgtg
     4141 tcataggcac caagtgacac catacatgaa cacgcgtcac aatatgactg gagaagggtt
     4201 ccacacctta tgctataaaa cgccccacac ccctcctcct tccttcgcag ttcaattcca
     4261 atatattcca ttctctctgt gtatttccct acctctccct tcaaggttag tcgatttctt
     4321 ctgtttttct tcttcgttct ttccatgaat tgtgtatgtt ctttgatcaa tacgatgttg
     4381 atttgattgt gttttgtttg gtttcatcga tcttcaattt tcataatcag attcagcttt
     4441 tattatcttt acaacaacgt ccttaatttg atgattcttt aatcgtagat ttgctctaat
     4501 tagagctttt tcatgtcaga tccctttaca acaagcctta attgttgatt cattaatcgt
     4561 agattagggc ttttttcatt gattacttca gatccgttaa acgtaaccat agatcagggc
     4621 tttttcatga attacttcag atccgttaaa caacagcctt attttttata cttctgtggt
     4681 ttttcaagaa attgttcaga tccgttgaca aaaagcctta ttcgttgatt ctatatcgtt
     4741 tttcgagaga tattgctcag atctgttagc aactgccttg tttgttgatt ctattgccgt
     4801 ggattagggt tttttttcac gagattgctt cagatccgta cttaagatta cgtaatggat
     4861 tttgattctg atttatctgt gattgttgac tcgacagcgg ccgcaccggc gcgccatggc
     4921 agatccaatg aagctactgt cttctatcga acaagcatgc gatatttgcc gacttaaaaa
     4981 gctcaagtgc tccaaagaaa aaccgaagtg cgccaagtgt ctgaagaaca actgggagtg
     5041 tcgctactct cccaaaacca aaagatctcc gctgactagg gcacatctga cagaagtgga
     5101 atcaaggcta gaaagactgg aacagctatt tctactgatt tttccaaggt cgagcgcccc
     5161 cccgaccgat gtcagcctgg gggacgagct ccacttagac ggcgaggacg tggcgatggc
     5221 gcatgccgac gcgctagacg atttcgatct ggacatgttg ggggacgggg attccccggg
     5281 tccgggattt accccccacg actccgcccc ctacggcgct ctggatatgg ccgacttcga
     5341 gtttgagcag atgtttaccg atgcccttgg aattgacgag tacggtgggg atccaattca
     5401 gcaagccact gcaggagtct cacaagacac ttcggaaaat cctaacaaaa caatagttcc
     5461 tgctgcatta ccacagctca cccctacctt ggtgtcactg ctggaggtga ttgaacccga
     5521 ggtgttgtat gcaggatatg atagctctgt tccagattca gcatggagaa ttatgaccac
     5581 actcaacatg ttaggtgggc gtcaagtgat tgcagcagtg aaatgggcaa aggcgatacc
     5641 aggcttcaga aacttacacc tggatgacca aatgaccctg ctacagtact catggatgtt
     5701 tctcatggca tttgccctgg gttggagatc atacagacaa tcaagtggaa acctgctctg
     5761 ctttgctcct gatctgatta ttaatgagca gagaatgtct ctaccctgca tgtatgacca
     5821 atgtaaacac atgctgtttg tctcctctga attacaaaga ttgcaggtat cctatgaaga
     5881 gtatctctgt atgaaaacct tactgcttct ctcctcagtt cctaaggaag gtctgaagag
     5941 ccaagagtta tttgatgaga ttcgaatgac ttatatcaaa gagctaggaa aagccatcgt
     6001 caaaagggaa gggaactcca gtcagaactg gcaacggttt taccaactga caaagcttct
     6061 ggactccatg catgaggtgg ttgagaatct ccttacctac tgcttccaga catttttgga
     6121 taagaccatg agtattgaat tcccagagat gttagctgaa atcatcacta atcagatacc
     6181 aaaatattca aatggaaata tcaaaaagct tctgtttcat caaaaatgac tcgacctaac
     6241 tgagtaagct agcttgttcg agtattatgg cattgggaaa actgtttttc ttgtaccatt
     6301 tgttgtgctt gtaatttact gtgtttttta ttcggttttc gctatcgaac tgtgaaatgg
     6361 aaatggatgg agaagagtta atgaatgata tggtcctttt gttcattctc aaattaatat
     6421 tatttgtttt ttctcttatt tgttgtgtgt tgaatttgaa attataagag atatgcaaac
     6481 attttgtttt gagtaaaaat gtgtcaaatc gtggcctcta atgaccgaag ttaatatgag
     6541 gagtaaaaca ctagatcccc aaacaagctt gaaactgaag gcgggaaacg acaatctgat
     6601 catgagcgga gaattaaggg agtcacgtta tgacccccgc cgatgacgcg ggacaagccg
     6661 ttttacgttt ggaactgaca tatgaaaata ttttttaatg cattttatga cttgccaatt
     6721 gattgacaac atgcatcaat ccgcggttat gactctctta agagagtcat gaattcgagc
     6781 gggaattgat cccccctcga cagcttgcat gccggtcgac tctagaggat ccgggtgaca
     6841 gccctccgac gggtgacagc cctccgacgg gtgacagccc tccgaattct agaggatccg
     6901 ggtgacagcc ctccgacggg tgacagccct ccgacgggtg acagccctcc gaattcgagc
     6961 tcagtacccg gggatctgtc gacctcgatc gagatcttcg caagaccctt cctctatata
     7021 aggaagttca tttcatttgg agaggacacg ctgaagctag tcgactctag cctcgaatcc
     7081 ttaggtacca caagtttgta caaaaaagct gaacgagaaa cgtaaaatga tataaatatc
     7141 aatatattaa attagatttt gcataaaaaa cagactacat aatactgtaa aacacaacat
     7201 atccagtcac tatggcggcc gcattaggca ccacaggctt tacactttat gcttccggct
     7261 cgtataatgt gtggattttg agttaggatc cgtcgagatt ttcaggagct aaggaagcta
     7321 aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg catcgtaaag
     7381 aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc gttcagctgg
     7441 atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat ccggccttta
     7501 ttcacattct tgcccgcctg atgaatgctc atccggaatt ccgtatggca atgaaagacg
     7561 gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat gagcaaactg
     7621 aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt ctacacatat
     7681 attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa gggtttattg
     7741 agaatatgtt tttcgtctca gccaatccct gggtgagttt caccagtttt gatttaaacg
     7801 tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat tatacgcaag
     7861 gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtttgt gatggcttcc
     7921 atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag ggcggggcgt
     7981 aaacgcgtgg atccggctta ctaaaagcca gataacagta tgcgtatttg cgcgctgatt
     8041 tttgcggtat aagaatatat actgatatgt atacccgaag tatgtcaaaa agaggtatgc
     8101 tatgaagcag cgtattacag tgacagttga cagcgacagc tatcagttgc tcaaggcata
     8161 tatgatgtca atatctccgg tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg
     8221 cgtgccgaac gctggaaagc ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt
     8281 gaaatgaacg gctcttttgc tgacgagaac aggggctggt gaaatgcagt ttaaggttta
     8341 cacctataaa agagagagcc gttatcgtct gtttgtggat gtacagagtg atattattga
     8401 cacgcccggg cgacggatgg tgatccccct ggccagtgca cgtctgctgt cagataaagt
     8461 ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa agctggcgca tgatgaccac
     8521 cgatatggcc agtgtgccgg tctccgttat cggggaagaa gtggctgatc tcagccaccg
     8581 cgaaaatgac atcaaaaacg ccattaacct gatgttctgg ggaatataaa tgtcaggctc
     8641 ccttatacac agccagtctg caggtcgacc atagtgactg gatatgttgt gttttacagt
     8701 attatgtagt ctgtttttta tgcaaaatct aatttaatat attgatattt atatcatttt
     8761 acgtttctcg ttcagctttc ttgtacaaag tggtcctagg ttcggttcga gtattatggc
     8821 attgggaaaa ctgtttttct tgtaccattt gttgtgcttg taatttactg tgttttttat
     8881 tcggttttcg ctatcgaact gtgaaatgga aatggatgga gaagagttaa tgaatgatat
     8941 ggtccttttg ttcattctca aattaatatt atttgttttt tctcttattt gttgtgtgtt
     9001 gaatttgaaa ttataagaga tatgcaaaca ttttgttttg agtaaaaatg tgtcaaatca
     9061 aggttccaga aggtaattat ccaagatgta gcatcaagaa tccaatgttt acgggaaaaa
     9121 ctatggaagt attatgtgag ctcagcaaga agcagatcaa tatgcggcac atatgcaacc
     9181 tatgttcaaa aatgaagaat gtacagatac aagatcctat actgccagaa tacgaagaag
     9241 aatacgtaga aattgaaaaa gaagaaccag gcgaagaaaa gaatcttgaa gacgtaagca
     9301 ctgacgacaa caatgaaaag aagaagataa ggtcggtgat tgtgaaagag acatagagga
     9361 cacatgtaag gtggaaaatg taagggcgga aagtaacctt atcacaaagg aatcttatcc
     9421 cccactactt atccttttat atttttccgt gtcatttttg cccttgagtt ttcctatata
     9481 aggaaccaag ttcggcattt gtgaaaacaa gaaaaaattt ggtgtaagct attttctttg
     9541 aagtactgag gatacaactt cagagaaatt tgtaagtttg tgatccgcaa acaaaaatgg
     9601 tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc
     9661 acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc
     9721 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg
     9781 cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg
     9841 ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga
     9901 tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg
     9961 agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc
    10021 agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc
    10081 tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg
    10141 tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca
    10201 tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg
    10261 ccgagggccg ccactccacc ggcggcatgg acgagctgta caagtgagat gtgcagatcg
    10321 ttcaaacatt tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat
    10381 tatcatataa tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac
    10441 gttatttatg agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat
    10501 agaaaacaaa atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt
    10561 actagatccg atgataagct gtcaaacatg agggacacga agtggtttaa actatcagtg
    10621 tttgacagga tatattggcg ggtaaaccta agagaaaaga gcgtttatta gaataatcgg
    10681 atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat ttgtatgtgc caaccacagg
    10741 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
    10801 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
    10861 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
    10921 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
    10981 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
    11041 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
    11101 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
    11161 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
    11221 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
    11281 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
    11341 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
    11401 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
    11461 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
    11521 tctgacagct aaaacaattc atccagtaaa atataatcga atattatgcg cacccgtggc
    11581 caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg
    11641 gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct
    11701 ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg
    11761 tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc
    11821 ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga
    11881 tcagcggtcc aatgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc
    11941 cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc
    12001 cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca
    12061 gcaagacgta gcccagcgcg tcgg
//
