LOCUS       sequence-464363-       15470 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GATEWAY compatible vector with a dexamethasone (DEX)-inducible 
            promoter driving in frame epitope fusion of 3xHA tag paired with 
            ccdb selection toxin and RUBY marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15470)
  TITLE     GATEWAY compatible vector with a promoter driving in frame epitope 
            fusion paired with ccdb selection toxin and FP marker
REFERENCE   2  (bases 1 to 15470)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: DEXsynth:ccdb-3xHA + RUBY
FEATURES             Location/Qualifiers
     source          1..15470
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     509..528
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        complement(join(928..933,934..950,951..956))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     rep_origin      1249..1443
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(1509..2582)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(3011..3640)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    3904..3928
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5180..5413
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     promoter        7024..7070
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     protein_bind    7114..7238
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(7273..7278,7279..7296,7297..7303)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             7357..8016
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(7423..7442)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             8358..8663
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     8542..8561
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(8704..8828)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             8857..8946
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     CDS             9761..13711
                     /label=RUBY
     CDS             11261..11317
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             12152..12208
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     terminator      13726..13978
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    14033..14057
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(14180..14768)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(14260..14279)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(15160..15470,1..880))
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
ORIGIN
        1 gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga
       61 aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata
      121 ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga
      181 cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg
      241 cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca
      301 agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta
      361 ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca
      421 acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc
      481 cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg
      541 cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc cacaggacgg
      601 gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg
      661 ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca
      721 acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat
      781 cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga
      841 cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt
      901 agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataaga tgtcaaacat
      961 gagaattctt gaaaatattg cgctgccatt tttggggtga ggccgttcgc ggccgagggg
     1021 cgcagcccct ggggggatgg gagctcggtg ccttgatgtg ggcgccggcg gtcgagtggc
     1081 gacggcgcgg cttgtccgcg ccctggtaga ttgcctggcc gtaggccagc catttttgag
     1141 cggccagcgg ccgcgatagg ccgacgcgaa gcggcggggc gtagggagcg cagcgaccga
     1201 agggtaggcg ctttttgcag ctcttcggct gtgcgctggc cagacagtta tgcacaggcc
     1261 aggcgggttt taagagtttt aataagtttt aaagagtttt aggcggaaaa atcgcctttt
     1321 ttctctttta tatcagtcac ttacatgtgt gaccggttcc caatgtacgg ctttgggttc
     1381 ccaatgtacg ggttccggtt cccaatgtac ggctttgggt tcccaatgta cgtgctatcc
     1441 acaggaaaga agctttttcg acctttttcc cctgctaggg caatttgccc tagcatctgc
     1501 tccgtacatt aggaaccggc ggatgcttcg ccctcgatca ggttgcggta gcgcatgact
     1561 aggatcgggc cagcctgccc cgcctcctcc ttcaaatcgt actccggcag gtcatttgac
     1621 ccgatcagct tgcgcacggt gaaacagaac ttcttgaact ctccggcgct gccactgcgt
     1681 tcgtagatcg tcttgaacaa ccatctggct tctgccttgc ctgcggcgcg gcgtgccagg
     1741 cggtagagaa aacggccgat gccgggatcg atcaaaaagt aatcggggtg aaccgtcagc
     1801 acgtccgggt tcttgccttc tgtgatctcg cggtacatcc aatcagctag ctcgatctcg
     1861 atgtactccg gccgcccggt ttcgctcttt acgatcttgt agcggctaat caaggcttca
     1921 ccctcggata ccgtcaccag gcggccgttc ttggccttct tcgtacgctg catggcaacg
     1981 tgcgtggtgt ttaaccgaat gcaggtttct accaggtcgt ctttctgctt tccgccatcg
     2041 gctcgccggc agaacttgag tacgtccgca acgtgtggac ggaacacgcg gccgggcttg
     2101 tctcccttcc cttcccggta tcggttcatg gattcggtta gatgggaaac cgccatcagt
     2161 accaggtcgt aatcccacac actggccatg ccggccggcc ctgcggaaac ctctacgtgc
     2221 ccgtctggaa gctcgtagcg gatcacctcg ccagctcgtc ggtcacgctt cgacagacgg
     2281 aaaacggcca cgtccatgat gctgcgacta tcgcgggtgc ccacgtcata gagcatcgga
     2341 acgaaaaaat ctggttgctc gtcgcccttg ggcggcttcc taatcgacga cgcaccggct
     2401 gccggcggtt gccgggattc tttgcggatt cgatcagcgg ccgcttgcca cgattcaccg
     2461 gggcgtgctt ctgcctcgat gcgttgccgc tgggcggcct gcgcggcctt caacttctcc
     2521 accaggtcat cacccagcgc cgcgccgatt tgtaccgggc cggatggttt gcgaccgctc
     2581 acgccgattc ctcgggcttg ggggttccag tgccattgca gggccggcag acaacccagc
     2641 cgcttacgcc tggccaaccg cccgttcctc cacacatggg gcattccacg gcgtcggtgc
     2701 ctggttgttc ttgattttcc atgccgcctc ctttagccgc taaaattcat ctactcattt
     2761 attcatttgc tcatttactc tggtagctgc gcgatgtatt cagatagcag ctcggtaatg
     2821 gtcttgcctt ggcgtaccgc gtacatcttc agcttggtgt gatcctccgc cggcaactga
     2881 aagttgaccc gcttcatggc tggcgtgtct gccaggctgg ccaacgttgc agccttgctg
     2941 ctgcgtgcgc tcggacggcc ggcacttagc gtgtttgtgc ttttgctcat tttctcttta
     3001 cctcattaac tcaaatgagt tttgatttaa tttcagcggc cagcgcctgg acctcgcggg
     3061 cagcgtcgcc ctcgggttct gattcaagaa cggttgtgcc ggcggcggca gtgcctgggt
     3121 agctcacgcg ctgcgtgata cgggactcaa gaatgggcag ctcgtacccg gccagcgcct
     3181 cggcaacctc accgccgatg cgcgtgcctt tgatcgcccg cgacacgaca aaggccgctt
     3241 gtagccttcc atccgtgacc tcaatgcgct gcttaaccag ctccaccagg tcggcggtgg
     3301 cccatatgtc gtaagggctt ggctgcaccg gaatcagcac gaagtcggct gccttgatcg
     3361 cggacacagc caagtccgcc gcctggggcg ctccgtcgat cactacgaag tcgcgccggc
     3421 cgatggcctt cacgtcgcgg tcaatcgtcg ggcggtcgat gccgacaacg gttagcggtt
     3481 gatcttcccg cacggccgcc caatcgcggg cactgccctg gggatcggaa tcgactaaca
     3541 gaacatcggc cccggcgagt tgcagggcgc gggctagatg ggttgcgatg gtcgtcttgc
     3601 ctgacccgcc tttctggtta agtacagcga taaccttcat gcgttcccct tgcgtatttg
     3661 tttatttact catcgcatca tatacgcagc gaccgcatga cgcaagctgt tttactcaaa
     3721 tacacatcac ctttttagac ggcggcgctc ggtttcttca gcggccaagc tggccggcca
     3781 ggccgccagc ttggcatcag acaaaccggc caggatttca tgcagccgca cggctgatgg
     3841 gctgcctgta tcgagtggtg attttgtgcc gagctgccgg tcggggagct gttggctggc
     3901 tggtggcagg atatattgtg gtgtaaacaa attgacgctt agacaactta ataacacatt
     3961 gcggacgttt ttaatgtact ggggtggatg cagtgggccc cactctgtgg aagacaatgc
     4021 ctccggagta acagataaag cgaagcttga aggtgcatgt gactccgtca agattacgaa
     4081 accgccaact accacgcaaa ttgcaattct caatttccta gaaggactct ccgaaaatgc
     4141 atccaatacc aaatattacc cgtgtcatag gcaccaagtg acaccataca tgaacacgcg
     4201 tcacaatatg actggagaag ggttccacac cttatgctat aaaacgcccc acacccctcc
     4261 tccttccttc gcagttcaat tccaatatat tccattctct ctgtgtattt ccctacctct
     4321 cccttcaagg ttagtcgatt tcttctgttt ttcttcttcg ttctttccat gaattgtgta
     4381 tgttctttga tcaatacgat gttgatttga ttgtgttttg tttggtttca tcgatcttca
     4441 attttcataa tcagattcag cttttattat ctttacaaca acgtccttaa tttgatgatt
     4501 ctttaatcgt agatttgctc taattagagc tttttcatgt cagatccctt tacaacaagc
     4561 cttaattgtt gattcattaa tcgtagatta gggctttttt cattgattac ttcagatccg
     4621 ttaaacgtaa ccatagatca gggctttttc atgaattact tcagatccgt taaacaacag
     4681 ccttattttt tatacttctg tggtttttca agaaattgtt cagatccgtt gacaaaaagc
     4741 cttattcgtt gattctatat cgtttttcga gagatattgc tcagatctgt tagcaactgc
     4801 cttgtttgtt gattctattg ccgtggatta gggttttttt tcacgagatt gcttcagatc
     4861 cgtacttaag attacgtaat ggattttgat tctgatttat ctgtgattgt tgactcgaca
     4921 gcggccgcac cggcgcgcca tggcagatcc aatgaagcta ctgtcttcta tcgaacaagc
     4981 atgcgatatt tgccgactta aaaagctcaa gtgctccaaa gaaaaaccga agtgcgccaa
     5041 gtgtctgaag aacaactggg agtgtcgcta ctctcccaaa accaaaagat ctccgctgac
     5101 tagggcacat ctgacagaag tggaatcaag gctagaaaga ctggaacagc tatttctact
     5161 gatttttcca aggtcgagcg cccccccgac cgatgtcagc ctgggggacg agctccactt
     5221 agacggcgag gacgtggcga tggcgcatgc cgacgcgcta gacgatttcg atctggacat
     5281 gttgggggac ggggattccc cgggtccggg atttaccccc cacgactccg ccccctacgg
     5341 cgctctggat atggccgact tcgagtttga gcagatgttt accgatgccc ttggaattga
     5401 cgagtacggt ggggatccaa ttcagcaagc cactgcagga gtctcacaag acacttcgga
     5461 aaatcctaac aaaacaatag ttcctgctgc attaccacag ctcaccccta ccttggtgtc
     5521 actgctggag gtgattgaac ccgaggtgtt gtatgcagga tatgatagct ctgttccaga
     5581 ttcagcatgg agaattatga ccacactcaa catgttaggt gggcgtcaag tgattgcagc
     5641 agtgaaatgg gcaaaggcga taccaggctt cagaaactta cacctggatg accaaatgac
     5701 cctgctacag tactcatgga tgtttctcat ggcatttgcc ctgggttgga gatcatacag
     5761 acaatcaagt ggaaacctgc tctgctttgc tcctgatctg attattaatg agcagagaat
     5821 gtctctaccc tgcatgtatg accaatgtaa acacatgctg tttgtctcct ctgaattaca
     5881 aagattgcag gtatcctatg aagagtatct ctgtatgaaa accttactgc ttctctcctc
     5941 agttcctaag gaaggtctga agagccaaga gttatttgat gagattcgaa tgacttatat
     6001 caaagagcta ggaaaagcca tcgtcaaaag ggaagggaac tccagtcaga actggcaacg
     6061 gttttaccaa ctgacaaagc ttctggactc catgcatgag gtggttgaga atctccttac
     6121 ctactgcttc cagacatttt tggataagac catgagtatt gaattcccag agatgttagc
     6181 tgaaatcatc actaatcaga taccaaaata ttcaaatgga aatatcaaaa agcttctgtt
     6241 tcatcaaaaa tgactcgacc taactgagta agctagcttg ttcgagtatt atggcattgg
     6301 gaaaactgtt tttcttgtac catttgttgt gcttgtaatt tactgtgttt tttattcggt
     6361 tttcgctatc gaactgtgaa atggaaatgg atggagaaga gttaatgaat gatatggtcc
     6421 ttttgttcat tctcaaatta atattatttg ttttttctct tatttgttgt gtgttgaatt
     6481 tgaaattata agagatatgc aaacattttg ttttgagtaa aaatgtgtca aatcgtggcc
     6541 tctaatgacc gaagttaata tgaggagtaa aacactagat ccccaaacaa gcttgaaact
     6601 gaaggcggga aacgacaatc tgatcatgag cggagaatta agggagtcac gttatgaccc
     6661 ccgccgatga cgcgggacaa gccgttttac gtttggaact gacatatgaa aatatttttt
     6721 aatgcatttt atgacttgcc aattgattga caacatgcat caatccgcgg ttatgactct
     6781 cttaagagag tcatgaattc gagcgggaat tgatcccccc tcgacagctt gcatgccggt
     6841 cgactctaga ggatccgggt gacagccctc cgacgggtga cagccctccg acgggtgaca
     6901 gccctccgaa ttctagagga tccgggtgac agccctccga cgggtgacag ccctccgacg
     6961 ggtgacagcc ctccgaattc gagctcagta cccggggatc tgtcgacctc gatcgagatc
     7021 ttcgcaagac ccttcctcta tataaggaag ttcatttcat ttggagagga cacgctgaag
     7081 ctagtcgact ctagcctcga atccttaggt accacaagtt tgtacaaaaa agctgaacga
     7141 gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa aaaacagact
     7201 acataatact gtaaaacaca acatatccag tcactatggc ggccctcgag ggccgcatta
     7261 ggcaccccag gctttacact ttatgcttcc ggctcgtata atgtgtggat tttgagttag
     7321 gatccgtcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat
     7381 accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt
     7441 gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta
     7501 aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat
     7561 gctcatccgg aattccgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt
     7621 cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa
     7681 taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt
     7741 gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt ctcagccaat
     7801 ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc
     7861 cccgttttca ccatgggcaa atattatacg caaggcgaca aggtgctgat gccgctggcg
     7921 attcaggttc atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct taatgaatta
     7981 caacagtact gcgatgagtg gcagggcggg gcgtaaagat ctggatccgg cttactaaaa
     8041 gccagataac agtatgcgta tttgcgcgct gatttttgcg gtataagaat atatactgat
     8101 atgtataccc gaagtatgtc aaaaagaggt atgctatgaa gcagcgtatt acagtgacag
     8161 ttgacagcga cagctatcag ttgctcaagg catatatgat gtcaatatct ccggtctggt
     8221 aagcacaacc atgcagaatg aagcccgtcg tctgcgtgcc gaacgctgga aagcggaaaa
     8281 tcaggaaggg atggctgagg tcgcccggtt tattgaaatg aacggctctt ttgctgacga
     8341 gaacaggggc tggtgaaatg cagtttaagg tttacaccta taaaagagag agccgttatc
     8401 gtctgtttgt ggatgtacag agtgatatta ttgacacgcc cgggcgacgg atggtgatcc
     8461 ccctggccag tgcacgtctg ctgtcagata aagtctcccg tgaactttac ccggtggtgc
     8521 atatcgggga tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg ccggtctccg
     8581 ttatcgggga agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta
     8641 acctgatgtt ctggggaata taaatgtcag gctcccttat acacagccag tctgcaggtc
     8701 gaccatagtg actggatatg ttgtgtttta cagtattatg tagtctgttt tttatgcaaa
     8761 atctaattta atatattgat atttatatca ttttacgttt ctcgttcagc tttcttgtac
     8821 aaagtggttg ataacagcgg gttaattaac atcttttacc catacgatgt tcctgactat
     8881 gcgggctatc cctatgacgt cccggactat gcaggatcct atccatatga cgttccagat
     8941 tacgctgctc agtgcagcgc ttagcctagg ttcggttcga gtattatggc attgggaaaa
     9001 ctgtttttct tgtaccattt gttgtgcttg taatttactg tgttttttat tcggttttcg
     9061 ctatcgaact gtgaaatgga aatggatgga gaagagttaa tgaatgatat ggtccttttg
     9121 ttcattctca aattaatatt atttgttttt tctcttattt gttgtgtgtt gaatttgaaa
     9181 ttataagaga tatgcaaaca ttttgttttg agtaaaaatg tgtcaaatca aggttccaga
     9241 aggtaattat ccaagatgta gcatcaagaa tccaatgttt acgggaaaaa ctatggaagt
     9301 attatgtgag ctcagcaaga agcagatcaa tatgcggcac atatgcaacc tatgttcaaa
     9361 aatgaagaat gtacagatac aagatcctat actgccagaa tacgaagaag aatacgtaga
     9421 aattgaaaaa gaagaaccag gcgaagaaaa gaatcttgaa gacgtaagca ctgacgacaa
     9481 caatgaaaag aagaagataa ggtcggtgat tgtgaaagag acatagagga cacatgtaag
     9541 gtggaaaatg taagggcgga aagtaacctt atcacaaagg aatcttatcc cccactactt
     9601 atccttttat atttttccgt gtcatttttg cccttgagtt ttcctatata aggaaccaag
     9661 ttcggcattt gtgaaaacaa gaaaaaattt ggtgtaagct attttctttg aagtactgag
     9721 gatacaactt cagagaaatt tgtaagtttg tgatccgcaa atggatcatg cgaccctcgc
     9781 catgatcctc gcgatctggt tcatcagctt ccacttcatc aagctgctgt tctcccagca
     9841 gaccaccaag ctgcttccgc caggaccaaa gccgcttccg atcatcggca acatccttga
     9901 ggtgggcaag aagccgcatc ggtccttcgc caacctcgcc aagattcacg gcccactcat
     9961 ttccctcaga ctcggctctg tgaccaccat cgttgtgtcc tctgccgacg tggccaaaga
    10021 gatgttcctc aagaaggatc acccgctctc caaccgcacg atcccgaata gtgttacagc
    10081 cggcgaccac cacaagctca ccatgtcttg gctcccggtg tctccgaagt ggcgcaactt
    10141 ccgcaagatt accgccgtgc atctgctctc cccacagaga ctcgatgcct gccagacatt
    10201 caggcacgcc aaggtgcagc agctctacga gtacgttcaa gagtgcgccc agaaaggcca
    10261 ggccgtggat attggcaagg ccgcctttac gaccagcctc aacctcctca gcaagctgtt
    10321 cttcagcgtc gagctggcgc accacaagtc ccataccagc caagagttca aagagctgat
    10381 ctggaacatc atggaagata taggcaagcc gaactacgcc gactacttcc cgattctcgg
    10441 ctgcgttgac ccatctggca ttagaagaag gctcgcctgc tccttcgaca agctgatcgc
    10501 cgtgttccag ggcatcatct gcgagagact cgccccagat tcctccacca caactaccac
    10561 caccaccgac gacgtgctcg atgtgctcct ccagctgttc aagcagaacg agctgacgat
    10621 gggcgagatc aaccacctcc tcgtggacat cttcgacgcc ggcaccgata ccacatcctc
    10681 cacattcgag tgggtgatga ccgagctgat ccgcaatcca gagatgatgg aaaaggccca
    10741 agaggaaatc aagcaggtcc tcggcaagga caagcagatc caagagtccg acatcatcaa
    10801 cctgccgtac ctccaggcga tcatcaaaga gacactccgc ctccatccgc cgaccgtgtt
    10861 cttgctccca agaaaggccg acaccgatgt cgagctgtac ggctacatcg tgccgaagga
    10921 tgcccagatc ctcgtgaacc tctgggccat tggcagggac ccaaacgcct ggcagaacgc
    10981 cgatattttc agcccagagc gcttcatcgg ctgcgagatc gatgttaagg gccgcgattt
    11041 cggcctcctt ccatttggcg ctggccgcag aatttgccca ggcatgaatc tcgccatcag
    11101 gatgctcacc ctcatgctcg ccacactcct ccagttcttc aactggaagc tcgaaggcga
    11161 catctccccg aaggacctcg acatggacga gaagttcggc attgcgctcc aaaagaccaa
    11221 gccgctcaag ctcatcccga ttccgcgcta cggtagcgga gctaccaatt ttagcctcct
    11281 taagcaggca ggtgatgtag aagagaaccc cgggcctatg aagatgatga acggcgagga
    11341 cgccaacgac cagatgatca aagagtcctt cttcatcacc cacggcaacc cgatcctcac
    11401 cgtcgaggat acacatccgc tcaggccgtt cttcgagaca tggcgcgaga agattttctc
    11461 caagaagccg aaggccatcc tcatcatctc cggccactgg gagacagtga agccaaccgt
    11521 gaacgccgtg cacatcaacg acaccatcca cgacttcgac gactacccag ccgccatgta
    11581 ccagttcaag tacccagctc caggcgagcc agagcttgcg agaaaggtgg aagagatcct
    11641 caagaagtcc gggttcgaga cagccgagac agaccaaaag aggggccttg atcacggcgc
    11701 ctgggttcca ctcatgctca tgtatccaga ggcggacatc ccggtgtgcc agctctcagt
    11761 tcagccacat ctcgacggca cctaccacta caatctcggc agagccctcg cgccgctcaa
    11821 gaatgatggc gtgctcatta ttggctccgg cagcgccaca catccactcg atgagacacc
    11881 gcactacttc gatggtgttg ccccttgggc cgctgccttc gattcttggc ttaggaaggc
    11941 cctcatcaac ggccgcttcg aggaagtgaa catctacgag agcaaggccc cgaactggaa
    12001 gctcgcccat ccatttccag agcacttcta cccgctccac gttgtgctcg gcgctgctgg
    12061 tgaaaagtgg aaggccgagc tgatccactc ctcctgggat catggcacac tttgccacgg
    12121 ctcctacaag ttcacctccg ccggatccgg agcaaccaac tttagcctgc tcaagcaagc
    12181 aggagatgtt gaggaaaatc ctggccccat gaccgccatc aagatgaaca ccaacggcga
    12241 gggcgagaca cagcacatcc tcatgatccc gttcatggcg cagggccacc tcaggccatt
    12301 tctcgaactc gccatgttcc tctacaagcg ctcccacgtg atcatcaccc tgctcacaac
    12361 tccgctcaat gccggcttcc tcaggcacct ccttcaccac cattcctact cctccagcgg
    12421 catcaggatc gtcgagctgc cattcaactc caccaaccac ggactcccac cgggcatcga
    12481 gaacaccgat aagctcacac tcccgctcgt ggtgtccctc ttccattcca ccatcagcct
    12541 cgatccgcac ctccgcgatt acatctccag gcatttcagc ccagccaggc caccactctg
    12601 cgtgatccat gatgtgttcc tcggctgggt tgaccaggtg gccaaggatg tgggctctac
    12661 aggcgtggtg ttcacaacag gcggcgctta tggcacatcc gcctacgtgt ccatctggaa
    12721 cgatctcccg caccagaact actccgacga ccaagagttc ccgctgccag gcttcccaga
    12781 gaaccataag ttccgcaggt cccagctcca tcggttcctc agatatgccg acggctccga
    12841 cgattggtcc aagtatttcc agccgcagct ccgccagtcc atgaagtctt ttggctggct
    12901 ctgcaactcc gtggaagaga tcgagacact cggcttctcc atcctccgca actacaccaa
    12961 gctgccgatc tggggcatcg gcccacttat tgcttcccca gtgcagcact cctcctccga
    13021 caacaattca acaggcgccg agttcgtgca gtggctcagc ctcaaagagc cggactccgt
    13081 cctctacatc tccttcggct cccagaacac gatcagcccg acgcagatga tggaactcgc
    13141 tgctggcctt gagtcctccg agaagccatt cctctgggtg atcagagccc cgttcggctt
    13201 cgacatcaac gaagagatgc gcccagagtg gctgccagag ggctttgagg aacgcatgaa
    13261 ggtgaagaaa cagggcaagc tcgtgtacaa gctcggcccg cagcttgaga tcctcaacca
    13321 tgaatccatc ggcggctttc tcacccactg cggatggaac agcatccttg agtctcttcg
    13381 cgagggcgtt ccgatgcttg gatggccact tgctgccgag caggcctaca acctcaagta
    13441 cctcgaagat gagatgggcg tcgcggttga gcttgctaga ggcctcgaag gcgagatctc
    13501 caaagagaag gtcaagcgca tcgtcgagat gatccttgag cgcaacgagg gctccaaagg
    13561 ctgggagatg aagaatcgcg ccgtggaaat gggcaaaaag ctcaaggacg ccgtgaacga
    13621 ggaaaaagag ctgaagggct cctccgtgaa ggcgatcgac gatttcctcg acgccgtcat
    13681 gcaggccaaa cttgagccaa gcctccagtg atagtgagat gtgcagatcg ttcaaacatt
    13741 tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa
    13801 tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg
    13861 agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa
    13921 atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt actagatccg
    13981 atgataagct gtcaaacatg agggacacga agtggtttaa actatcagtg tttgacagga
    14041 tatattggcg ggtaaaccta agagaaaaga gcgtttatta gaataatcgg atatttaaaa
    14101 gggcgtgaaa aggtttatcc gttcgtccat ttgtatgtgc caaccacagg aaccgtaaaa
    14161 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
    14221 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
    14281 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
    14341 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
    14401 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
    14461 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
    14521 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
    14581 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
    14641 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
    14701 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
    14761 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
    14821 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
    14881 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagct
    14941 aaaacaattc atccagtaaa atataatcga atattatgcg cacccgtggc caggacccaa
    15001 cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct
    15061 gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg
    15121 gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct
    15181 ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca
    15241 tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc
    15301 aatgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc
    15361 gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc
    15421 gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca
//
