LOCUS       sequence-464366-       13172 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GATEWAY compatible vector with soybean Ubiquitin (GmUbi) promoter 
            driving in frame epitope fusion of ccdb selection toxin and RUBY 
            marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13172)
  TITLE     GATEWAY compatible vector with a promoter driving in frame epitope 
            fusion paired with ccdb selection toxin and FP marker
REFERENCE   2  (bases 1 to 13172)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Ubi:ccdb + RUBY
FEATURES             Location/Qualifiers
     source          1..13172
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     485..504
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        complement(join(904..909,910..926,927..932))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     rep_origin      1225..1419
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(1485..2558)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(2987..3616)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    3880..3904
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     protein_bind    4938..5062
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(5087..5092,5093..5110,5111..5117)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             5171..5830
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(5237..5256)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             6172..6477
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     6356..6375
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(6518..6642)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             7439..11389
                     /label=RUBY
     CDS             8939..8995
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             9830..9886
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     terminator      11404..11656
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    11711..11735
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(11858..12446)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11938..11957)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12838..13172,1..856))
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
ORIGIN
        1 ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga
       61 cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg
      121 tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc
      181 ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg
      241 cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct
      301 tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg
      361 ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg
      421 ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc
      481 catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg
      541 ccacgatgcg tccggcgtag aggatccaca ggacgggtgt ggtcgccatg atcgcgtagt
      601 cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca
      661 gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc
      721 catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg
      781 gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg
      841 cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac
      901 cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaaa atattgcgct
      961 gccatttttg gggtgaggcc gttcgcggcc gaggggcgca gcccctgggg ggatgggagc
     1021 tcggtgcctt gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg tccgcgccct
     1081 ggtagattgc ctggccgtag gccagccatt tttgagcggc cagcggccgc gataggccga
     1141 cgcgaagcgg cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt ttgcagctct
     1201 tcggctgtgc gctggccaga cagttatgca caggccaggc gggttttaag agttttaata
     1261 agttttaaag agttttaggc ggaaaaatcg ccttttttct cttttatatc agtcacttac
     1321 atgtgtgacc ggttcccaat gtacggcttt gggttcccaa tgtacgggtt ccggttccca
     1381 atgtacggct ttgggttccc aatgtacgtg ctatccacag gaaagaagct ttttcgacct
     1441 ttttcccctg ctagggcaat ttgccctagc atctgctccg tacattagga accggcggat
     1501 gcttcgccct cgatcaggtt gcggtagcgc atgactagga tcgggccagc ctgccccgcc
     1561 tcctccttca aatcgtactc cggcaggtca tttgacccga tcagcttgcg cacggtgaaa
     1621 cagaacttct tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt gaacaaccat
     1681 ctggcttctg ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg gccgatgccg
     1741 ggatcgatca aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt gccttctgtg
     1801 atctcgcggt acatccaatc agctagctcg atctcgatgt actccggccg cccggtttcg
     1861 ctctttacga tcttgtagcg gctaatcaag gcttcaccct cggataccgt caccaggcgg
     1921 ccgttcttgg ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa ccgaatgcag
     1981 gtttctacca ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa cttgagtacg
     2041 tccgcaacgt gtggacggaa cacgcggccg ggcttgtctc ccttcccttc ccggtatcgg
     2101 ttcatggatt cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc ccacacactg
     2161 gccatgccgg ccggccctgc ggaaacctct acgtgcccgt ctggaagctc gtagcggatc
     2221 acctcgccag ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc catgatgctg
     2281 cgactatcgc gggtgcccac gtcatagagc atcggaacga aaaaatctgg ttgctcgtcg
     2341 cccttgggcg gcttcctaat cgacgacgca ccggctgccg gcggttgccg ggattctttg
     2401 cggattcgat cagcggccgc ttgccacgat tcaccggggc gtgcttctgc ctcgatgcgt
     2461 tgccgctggg cggcctgcgc ggccttcaac ttctccacca ggtcatcacc cagcgccgcg
     2521 ccgatttgta ccgggccgga tggtttgcga ccgctcacgc cgattcctcg ggcttggggg
     2581 ttccagtgcc attgcagggc cggcagacaa cccagccgct tacgcctggc caaccgcccg
     2641 ttcctccaca catggggcat tccacggcgt cggtgcctgg ttgttcttga ttttccatgc
     2701 cgcctccttt agccgctaaa attcatctac tcatttattc atttgctcat ttactctggt
     2761 agctgcgcga tgtattcaga tagcagctcg gtaatggtct tgccttggcg taccgcgtac
     2821 atcttcagct tggtgtgatc ctccgccggc aactgaaagt tgacccgctt catggctggc
     2881 gtgtctgcca ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg acggccggca
     2941 cttagcgtgt ttgtgctttt gctcattttc tctttacctc attaactcaa atgagttttg
     3001 atttaatttc agcggccagc gcctggacct cgcgggcagc gtcgccctcg ggttctgatt
     3061 caagaacggt tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc gtgatacggg
     3121 actcaagaat gggcagctcg tacccggcca gcgcctcggc aacctcaccg ccgatgcgcg
     3181 tgcctttgat cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc gtgacctcaa
     3241 tgcgctgctt aaccagctcc accaggtcgg cggtggccca tatgtcgtaa gggcttggct
     3301 gcaccggaat cagcacgaag tcggctgcct tgatcgcgga cacagccaag tccgccgcct
     3361 ggggcgctcc gtcgatcact acgaagtcgc gccggccgat ggccttcacg tcgcggtcaa
     3421 tcgtcgggcg gtcgatgccg acaacggtta gcggttgatc ttcccgcacg gccgcccaat
     3481 cgcgggcact gccctgggga tcggaatcga ctaacagaac atcggccccg gcgagttgca
     3541 gggcgcgggc tagatgggtt gcgatggtcg tcttgcctga cccgcctttc tggttaagta
     3601 cagcgataac cttcatgcgt tccccttgcg tatttgttta tttactcatc gcatcatata
     3661 cgcagcgacc gcatgacgca agctgtttta ctcaaataca catcaccttt ttagacggcg
     3721 gcgctcggtt tcttcagcgg ccaagctggc cggccaggcc gccagcttgg catcagacaa
     3781 accggccagg atttcatgca gccgcacggc tgatgggctg cctgtatcga gtggtgattt
     3841 tgtgccgagc tgccggtcgg ggagctgttg gctggctggt ggcaggatat attgtggtgt
     3901 aaacaaattg acgcttagac aacttaataa cacattgcgg acgtttttaa tgtactgggg
     3961 tggatgcagt gggccccact ctgtggaaga caatgccccg cggtccggag taacagataa
     4021 agcgaagctt gaaggtgcat gtgactccgt caagattacg aaaccgccaa ctaccacgca
     4081 aattgcaatt ctcaatttcc tagaaggact ctccgaaaat gcatccaata ccaaatatta
     4141 cccgtgtcat aggcaccaag tgacaccata catgaacacg cgtcacaata tgactggaga
     4201 agggttccac accttatgct ataaaacgcc ccacacccct cctccttcct tcgcagttca
     4261 attccaatat attccattct ctctgtgtat ttccctacct ctcccttcaa ggttagtcga
     4321 tttcttctgt ttttcttctt cgttctttcc atgaattgtg tatgttcttt gatcaatacg
     4381 atgttgattt gattgtgttt tgtttggttt catcgatctt caattttcat aatcagattc
     4441 agcttttatt atctttacaa caacgtcctt aatttgatga ttctttaatc gtagatttgc
     4501 tctaattaga gctttttcat gtcagatccc tttacaacaa gccttaattg ttgattcatt
     4561 aatcgtagat tagggctttt ttcattgatt acttcagatc cgttaaacgt aaccatagat
     4621 cagggctttt tcatgaatta cttcagatcc gttaaacaac agccttattt tttatacttc
     4681 tgtggttttt caagaaattg ttcagatccg ttgacaaaaa gccttattcg ttgattctat
     4741 atcgtttttc gagagatatt gctcagatct gttagcaact gccttgtttg ttgattctat
     4801 tgccgtggat tagggttttt tttcacgaga ttgcttcaga tccgtactta agattacgta
     4861 atggattttg attctgattt atctgtgatt gttgactcga cagcggccgc acccccgggg
     4921 gcgcgccctt aggtaccaca agtttgtaca aaaaagctga acgagaaacg taaaatgata
     4981 taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa tactgtaaaa
     5041 cacaacatat ccagtcacta tggcggccgc attaggcacc ccaggcttta cactttatgc
     5101 ttccggctcg tataatgtgt ggattttgag ttaggatccg tcgagatttt caggagctaa
     5161 ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca
     5221 tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt
     5281 tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc
     5341 ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaattcc gtatggcaat
     5401 gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga
     5461 gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct
     5521 acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg
     5581 gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga
     5641 tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgg gcaaatatta
     5701 tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga
     5761 tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggg
     5821 cggggcgtaa acgcgtggat ccggcttact aaaagccaga taacagtatg cgtatttgcg
     5881 cgctgatttt tgcggtataa gaatatatac tgatatgtat acccgaagta tgtcaaaaag
     5941 aggtatgcta tgaagcagcg tattacagtg acagttgaca gcgacagcta tcagttgctc
     6001 aaggcatata tgatgtcaat atctccggtc tggtaagcac aaccatgcag aatgaagccc
     6061 gtcgtctgcg tgccgaacgc tggaaagcgg aaaatcagga agggatggct gaggtcgccc
     6121 ggtttattga aatgaacggc tcttttgctg acgagaacag gggctggtga aatgcagttt
     6181 aaggtttaca cctataaaag agagagccgt tatcgtctgt ttgtggatgt acagagtgat
     6241 attattgaca cgcccgggcg acggatggtg atccccctgg ccagtgcacg tctgctgtca
     6301 gataaagtct cccgtgaact ttacccggtg gtgcatatcg gggatgaaag ctggcgcatg
     6361 atgaccaccg atatggccag tgtgccggtc tccgttatcg gggaagaagt ggctgatctc
     6421 agccaccgcg aaaatgacat caaaaacgcc attaacctga tgttctgggg aatataaatg
     6481 tcaggctccc ttatacacag ccagtctgca ggtcgaccat agtgactgga tatgttgtgt
     6541 tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat tgatatttat
     6601 atcattttac gtttctcgtt cagctttctt gtacaaagtg gtcctaggtt cggttcgagt
     6661 attatggcat tgggaaaact gtttttcttg taccatttgt tgtgcttgta atttactgtg
     6721 ttttttattc ggttttcgct atcgaactgt gaaatggaaa tggatggaga agagttaatg
     6781 aatgatatgg tccttttgtt cattctcaaa ttaatattat ttgttttttc tcttatttgt
     6841 tgtgtgttga atttgaaatt ataagagata tgcaaacatt ttgttttgag taaaaatgtg
     6901 tcaaatcaag gttccagaag gtaattatcc aagatgtagc atcaagaatc caatgtttac
     6961 gggaaaaact atggaagtat tatgtgagct cagcaagaag cagatcaata tgcggcacat
     7021 atgcaaccta tgttcaaaaa tgaagaatgt acagatacaa gatcctatac tgccagaata
     7081 cgaagaagaa tacgtagaaa ttgaaaaaga agaaccaggc gaagaaaaga atcttgaaga
     7141 cgtaagcact gacgacaaca atgaaaagaa gaagataagg tcggtgattg tgaaagagac
     7201 atagaggaca catgtaaggt ggaaaatgta agggcggaaa gtaaccttat cacaaaggaa
     7261 tcttatcccc cactacttat ccttttatat ttttccgtgt catttttgcc cttgagtttt
     7321 cctatataag gaaccaagtt cggcatttgt gaaaacaaga aaaaatttgg tgtaagctat
     7381 tttctttgaa gtactgagga tacaacttca gagaaatttg taagtttgtg atccgcaaat
     7441 ggatcatgcg accctcgcca tgatcctcgc gatctggttc atcagcttcc acttcatcaa
     7501 gctgctgttc tcccagcaga ccaccaagct gcttccgcca ggaccaaagc cgcttccgat
     7561 catcggcaac atccttgagg tgggcaagaa gccgcatcgg tccttcgcca acctcgccaa
     7621 gattcacggc ccactcattt ccctcagact cggctctgtg accaccatcg ttgtgtcctc
     7681 tgccgacgtg gccaaagaga tgttcctcaa gaaggatcac ccgctctcca accgcacgat
     7741 cccgaatagt gttacagccg gcgaccacca caagctcacc atgtcttggc tcccggtgtc
     7801 tccgaagtgg cgcaacttcc gcaagattac cgccgtgcat ctgctctccc cacagagact
     7861 cgatgcctgc cagacattca ggcacgccaa ggtgcagcag ctctacgagt acgttcaaga
     7921 gtgcgcccag aaaggccagg ccgtggatat tggcaaggcc gcctttacga ccagcctcaa
     7981 cctcctcagc aagctgttct tcagcgtcga gctggcgcac cacaagtccc ataccagcca
     8041 agagttcaaa gagctgatct ggaacatcat ggaagatata ggcaagccga actacgccga
     8101 ctacttcccg attctcggct gcgttgaccc atctggcatt agaagaaggc tcgcctgctc
     8161 cttcgacaag ctgatcgccg tgttccaggg catcatctgc gagagactcg ccccagattc
     8221 ctccaccaca actaccacca ccaccgacga cgtgctcgat gtgctcctcc agctgttcaa
     8281 gcagaacgag ctgacgatgg gcgagatcaa ccacctcctc gtggacatct tcgacgccgg
     8341 caccgatacc acatcctcca cattcgagtg ggtgatgacc gagctgatcc gcaatccaga
     8401 gatgatggaa aaggcccaag aggaaatcaa gcaggtcctc ggcaaggaca agcagatcca
     8461 agagtccgac atcatcaacc tgccgtacct ccaggcgatc atcaaagaga cactccgcct
     8521 ccatccgccg accgtgttct tgctcccaag aaaggccgac accgatgtcg agctgtacgg
     8581 ctacatcgtg ccgaaggatg cccagatcct cgtgaacctc tgggccattg gcagggaccc
     8641 aaacgcctgg cagaacgccg atattttcag cccagagcgc ttcatcggct gcgagatcga
     8701 tgttaagggc cgcgatttcg gcctccttcc atttggcgct ggccgcagaa tttgcccagg
     8761 catgaatctc gccatcagga tgctcaccct catgctcgcc acactcctcc agttcttcaa
     8821 ctggaagctc gaaggcgaca tctccccgaa ggacctcgac atggacgaga agttcggcat
     8881 tgcgctccaa aagaccaagc cgctcaagct catcccgatt ccgcgctacg gtagcggagc
     8941 taccaatttt agcctcctta agcaggcagg tgatgtagaa gagaaccccg ggcctatgaa
     9001 gatgatgaac ggcgaggacg ccaacgacca gatgatcaaa gagtccttct tcatcaccca
     9061 cggcaacccg atcctcaccg tcgaggatac acatccgctc aggccgttct tcgagacatg
     9121 gcgcgagaag attttctcca agaagccgaa ggccatcctc atcatctccg gccactggga
     9181 gacagtgaag ccaaccgtga acgccgtgca catcaacgac accatccacg acttcgacga
     9241 ctacccagcc gccatgtacc agttcaagta cccagctcca ggcgagccag agcttgcgag
     9301 aaaggtggaa gagatcctca agaagtccgg gttcgagaca gccgagacag accaaaagag
     9361 gggccttgat cacggcgcct gggttccact catgctcatg tatccagagg cggacatccc
     9421 ggtgtgccag ctctcagttc agccacatct cgacggcacc taccactaca atctcggcag
     9481 agccctcgcg ccgctcaaga atgatggcgt gctcattatt ggctccggca gcgccacaca
     9541 tccactcgat gagacaccgc actacttcga tggtgttgcc ccttgggccg ctgccttcga
     9601 ttcttggctt aggaaggccc tcatcaacgg ccgcttcgag gaagtgaaca tctacgagag
     9661 caaggccccg aactggaagc tcgcccatcc atttccagag cacttctacc cgctccacgt
     9721 tgtgctcggc gctgctggtg aaaagtggaa ggccgagctg atccactcct cctgggatca
     9781 tggcacactt tgccacggct cctacaagtt cacctccgcc ggatccggag caaccaactt
     9841 tagcctgctc aagcaagcag gagatgttga ggaaaatcct ggccccatga ccgccatcaa
     9901 gatgaacacc aacggcgagg gcgagacaca gcacatcctc atgatcccgt tcatggcgca
     9961 gggccacctc aggccatttc tcgaactcgc catgttcctc tacaagcgct cccacgtgat
    10021 catcaccctg ctcacaactc cgctcaatgc cggcttcctc aggcacctcc ttcaccacca
    10081 ttcctactcc tccagcggca tcaggatcgt cgagctgcca ttcaactcca ccaaccacgg
    10141 actcccaccg ggcatcgaga acaccgataa gctcacactc ccgctcgtgg tgtccctctt
    10201 ccattccacc atcagcctcg atccgcacct ccgcgattac atctccaggc atttcagccc
    10261 agccaggcca ccactctgcg tgatccatga tgtgttcctc ggctgggttg accaggtggc
    10321 caaggatgtg ggctctacag gcgtggtgtt cacaacaggc ggcgcttatg gcacatccgc
    10381 ctacgtgtcc atctggaacg atctcccgca ccagaactac tccgacgacc aagagttccc
    10441 gctgccaggc ttcccagaga accataagtt ccgcaggtcc cagctccatc ggttcctcag
    10501 atatgccgac ggctccgacg attggtccaa gtatttccag ccgcagctcc gccagtccat
    10561 gaagtctttt ggctggctct gcaactccgt ggaagagatc gagacactcg gcttctccat
    10621 cctccgcaac tacaccaagc tgccgatctg gggcatcggc ccacttattg cttccccagt
    10681 gcagcactcc tcctccgaca acaattcaac aggcgccgag ttcgtgcagt ggctcagcct
    10741 caaagagccg gactccgtcc tctacatctc cttcggctcc cagaacacga tcagcccgac
    10801 gcagatgatg gaactcgctg ctggccttga gtcctccgag aagccattcc tctgggtgat
    10861 cagagccccg ttcggcttcg acatcaacga agagatgcgc ccagagtggc tgccagaggg
    10921 ctttgaggaa cgcatgaagg tgaagaaaca gggcaagctc gtgtacaagc tcggcccgca
    10981 gcttgagatc ctcaaccatg aatccatcgg cggctttctc acccactgcg gatggaacag
    11041 catccttgag tctcttcgcg agggcgttcc gatgcttgga tggccacttg ctgccgagca
    11101 ggcctacaac ctcaagtacc tcgaagatga gatgggcgtc gcggttgagc ttgctagagg
    11161 cctcgaaggc gagatctcca aagagaaggt caagcgcatc gtcgagatga tccttgagcg
    11221 caacgagggc tccaaaggct gggagatgaa gaatcgcgcc gtggaaatgg gcaaaaagct
    11281 caaggacgcc gtgaacgagg aaaaagagct gaagggctcc tccgtgaagg cgatcgacga
    11341 tttcctcgac gccgtcatgc aggccaaact tgagccaagc ctccagtgat agtgagatgt
    11401 gcagatcgtt caaacatttg gcaataaagt ttcttaagat tgaatcctgt tgccggtctt
    11461 gcgatgatta tcatataatt tctgttgaat tacgttaagc atgtaataat taacatgtaa
    11521 tgcatgacgt tatttatgag atgggttttt atgattagag tcccgcaatt atacatttaa
    11581 tacgcgatag aaaacaaaat atagcgcgca aactaggata aattatcgcg cgcggtgtca
    11641 tctatgttac tagatccgat gataagctgt caaacatgag ggacacgaag tggtttaaac
    11701 tatcagtgtt tgacaggata tattggcggg taaacctaag agaaaagagc gtttattaga
    11761 ataatcggat atttaaaagg gcgtgaaaag gtttatccgt tcgtccattt gtatgtgcca
    11821 accacaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
    11881 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
    11941 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
    12001 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
    12061 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
    12121 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
    12181 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
    12241 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
    12301 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
    12361 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
    12421 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
    12481 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
    12541 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
    12601 aaacttggtc tgacagctaa aacaattcat ccagtaaaat ataatcgaat attatgcgca
    12661 cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg
    12721 acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt
    12781 gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca
    12841 ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag
    12901 ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc
    12961 cgtgacgatc agcggtccaa tgatcgaagt taggctggta agagccgcga gcgatccttg
    13021 aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat
    13081 cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc
    13141 gaacgccagc aagacgtagc ccagcgcgtc gg
//
