LOCUS       sequence-464376-       16194 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GATEWAY compatible vector with a dexamethasone (DEX)-inducible 
            promoter driving in frame epitope fusion miniTurbo-ID paired with 
            ccdb selection toxin and RUBY marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16194)
  TITLE     GATEWAY compatible vector with a promoter driving in frame epitope 
            fusion paired with ccdb selection toxin and FP marker
REFERENCE   2  (bases 1 to 16194)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: DEXsynth:mTb-ccdb+ RUBY
FEATURES             Location/Qualifiers
     source          1..16194
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             709..1014
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     893..912
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1055..1179)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1979..5929
                     /label=RUBY
     CDS             3479..3535
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4370..4426
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     terminator      5944..6196
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    6251..6275
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(6398..6986)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6478..6497)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7378..8568)
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     8197..8216
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        complement(join(8616..8621,8622..8638,8639..8644))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     rep_origin      8937..9131
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9197..10270)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10699..11328)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    11592..11616
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             12868..13101
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     promoter        14712..14758
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     CDS             15609..15650
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    15669..15793
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(15818..15823,15824..15841,15842..15848)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             join(15902..16194,1..367)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(15968..15987)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 aactgaaacg ttttcatcgc tctggagtga ataccacgac gatttccggc agtttctaca
       61 catatattcg caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt
      121 tattgagaat atgtttttcg tctcagccaa tccctgggtg agtttcacca gttttgattt
      181 aaacgtggcc aatatggaca acttcttcgc ccccgttttc accatgggca aatattatac
      241 gcaaggcgac aaggtgctga tgccgctggc gattcaggtt catcatgccg tttgtgatgg
      301 cttccatgtc ggcagaatgc ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg
      361 ggcgtaaacg cgtggatccg gcttactaaa agccagataa cagtatgcgt atttgcgcgc
      421 tgatttttgc ggtataagaa tatatactga tatgtatacc cgaagtatgt caaaaagagg
      481 tatgctatga agcagcgtat tacagtgaca gttgacagcg acagctatca gttgctcaag
      541 gcatatatga tgtcaatatc tccggtctgg taagcacaac catgcagaat gaagcccgtc
      601 gtctgcgtgc cgaacgctgg aaagcggaaa atcaggaagg gatggctgag gtcgcccggt
      661 ttattgaaat gaacggctct tttgctgacg agaacagggg ctggtgaaat gcagtttaag
      721 gtttacacct ataaaagaga gagccgttat cgtctgtttg tggatgtaca gagtgatatt
      781 attgacacgc ccgggcgacg gatggtgatc cccctggcca gtgcacgtct gctgtcagat
      841 aaagtctccc gtgaacttta cccggtggtg catatcgggg atgaaagctg gcgcatgatg
      901 accaccgata tggccagtgt gccggtctcc gttatcgggg aagaagtggc tgatctcagc
      961 caccgcgaaa atgacatcaa aaacgccatt aacctgatgt tctggggaat ataaatgtca
     1021 ggctccctta tacacagcca gtctgcaggt cgaccatagt gactggatat gttgtgtttt
     1081 acagtattat gtagtctgtt ttttatgcaa aatctaattt aatatattga tatttatatc
     1141 attttacgtt tctcgttcag ctttcttgta caaagtggtc ctaggtgatt cggttcgagt
     1201 attatggcat tgggaaaact gtttttcttg taccatttgt tgtgcttgta atttactgtg
     1261 ttttttattc ggttttcgct atcgaactgt gaaatggaaa tggatggaga agagttaatg
     1321 aatgatatgg tccttttgtt cattctcaaa ttaatattat ttgttttttc tcttatttgt
     1381 tgtgtgttga atttgaaatt ataagagata tgcaaacatt ttgttttgag taaaaatgtg
     1441 tcaaatcaag gttccagaag gtaattatcc aagatgtagc atcaagaatc caatgtttac
     1501 gggaaaaact atggaagtat tatgtgagct cagcaagaag cagatcaata tgcggcacat
     1561 atgcaaccta tgttcaaaaa tgaagaatgt acagatacaa gatcctatac tgccagaata
     1621 cgaagaagaa tacgtagaaa ttgaaaaaga agaaccaggc gaagaaaaga atcttgaaga
     1681 cgtaagcact gacgacaaca atgaaaagaa gaagataagg tcggtgattg tgaaagagac
     1741 atagaggaca catgtaaggt ggaaaatgta agggcggaaa gtaaccttat cacaaaggaa
     1801 tcttatcccc cactacttat ccttttatat ttttccgtgt catttttgcc cttgagtttt
     1861 cctatataag gaaccaagtt cggcatttgt gaaaacaaga aaaaatttgg tgtaagctat
     1921 tttctttgaa gtactgagga tacaacttca gagaaatttg taagtttgtg atccgcaaat
     1981 ggatcatgcg accctcgcca tgatcctcgc gatctggttc atcagcttcc acttcatcaa
     2041 gctgctgttc tcccagcaga ccaccaagct gcttccgcca ggaccaaagc cgcttccgat
     2101 catcggcaac atccttgagg tgggcaagaa gccgcatcgg tccttcgcca acctcgccaa
     2161 gattcacggc ccactcattt ccctcagact cggctctgtg accaccatcg ttgtgtcctc
     2221 tgccgacgtg gccaaagaga tgttcctcaa gaaggatcac ccgctctcca accgcacgat
     2281 cccgaatagt gttacagccg gcgaccacca caagctcacc atgtcttggc tcccggtgtc
     2341 tccgaagtgg cgcaacttcc gcaagattac cgccgtgcat ctgctctccc cacagagact
     2401 cgatgcctgc cagacattca ggcacgccaa ggtgcagcag ctctacgagt acgttcaaga
     2461 gtgcgcccag aaaggccagg ccgtggatat tggcaaggcc gcctttacga ccagcctcaa
     2521 cctcctcagc aagctgttct tcagcgtcga gctggcgcac cacaagtccc ataccagcca
     2581 agagttcaaa gagctgatct ggaacatcat ggaagatata ggcaagccga actacgccga
     2641 ctacttcccg attctcggct gcgttgaccc atctggcatt agaagaaggc tcgcctgctc
     2701 cttcgacaag ctgatcgccg tgttccaggg catcatctgc gagagactcg ccccagattc
     2761 ctccaccaca actaccacca ccaccgacga cgtgctcgat gtgctcctcc agctgttcaa
     2821 gcagaacgag ctgacgatgg gcgagatcaa ccacctcctc gtggacatct tcgacgccgg
     2881 caccgatacc acatcctcca cattcgagtg ggtgatgacc gagctgatcc gcaatccaga
     2941 gatgatggaa aaggcccaag aggaaatcaa gcaggtcctc ggcaaggaca agcagatcca
     3001 agagtccgac atcatcaacc tgccgtacct ccaggcgatc atcaaagaga cactccgcct
     3061 ccatccgccg accgtgttct tgctcccaag aaaggccgac accgatgtcg agctgtacgg
     3121 ctacatcgtg ccgaaggatg cccagatcct cgtgaacctc tgggccattg gcagggaccc
     3181 aaacgcctgg cagaacgccg atattttcag cccagagcgc ttcatcggct gcgagatcga
     3241 tgttaagggc cgcgatttcg gcctccttcc atttggcgct ggccgcagaa tttgcccagg
     3301 catgaatctc gccatcagga tgctcaccct catgctcgcc acactcctcc agttcttcaa
     3361 ctggaagctc gaaggcgaca tctccccgaa ggacctcgac atggacgaga agttcggcat
     3421 tgcgctccaa aagaccaagc cgctcaagct catcccgatt ccgcgctacg gtagcggagc
     3481 taccaatttt agcctcctta agcaggcagg tgatgtagaa gagaaccccg ggcctatgaa
     3541 gatgatgaac ggcgaggacg ccaacgacca gatgatcaaa gagtccttct tcatcaccca
     3601 cggcaacccg atcctcaccg tcgaggatac acatccgctc aggccgttct tcgagacatg
     3661 gcgcgagaag attttctcca agaagccgaa ggccatcctc atcatctccg gccactggga
     3721 gacagtgaag ccaaccgtga acgccgtgca catcaacgac accatccacg acttcgacga
     3781 ctacccagcc gccatgtacc agttcaagta cccagctcca ggcgagccag agcttgcgag
     3841 aaaggtggaa gagatcctca agaagtccgg gttcgagaca gccgagacag accaaaagag
     3901 gggccttgat cacggcgcct gggttccact catgctcatg tatccagagg cggacatccc
     3961 ggtgtgccag ctctcagttc agccacatct cgacggcacc taccactaca atctcggcag
     4021 agccctcgcg ccgctcaaga atgatggcgt gctcattatt ggctccggca gcgccacaca
     4081 tccactcgat gagacaccgc actacttcga tggtgttgcc ccttgggccg ctgccttcga
     4141 ttcttggctt aggaaggccc tcatcaacgg ccgcttcgag gaagtgaaca tctacgagag
     4201 caaggccccg aactggaagc tcgcccatcc atttccagag cacttctacc cgctccacgt
     4261 tgtgctcggc gctgctggtg aaaagtggaa ggccgagctg atccactcct cctgggatca
     4321 tggcacactt tgccacggct cctacaagtt cacctccgcc ggatccggag caaccaactt
     4381 tagcctgctc aagcaagcag gagatgttga ggaaaatcct ggccccatga ccgccatcaa
     4441 gatgaacacc aacggcgagg gcgagacaca gcacatcctc atgatcccgt tcatggcgca
     4501 gggccacctc aggccatttc tcgaactcgc catgttcctc tacaagcgct cccacgtgat
     4561 catcaccctg ctcacaactc cgctcaatgc cggcttcctc aggcacctcc ttcaccacca
     4621 ttcctactcc tccagcggca tcaggatcgt cgagctgcca ttcaactcca ccaaccacgg
     4681 actcccaccg ggcatcgaga acaccgataa gctcacactc ccgctcgtgg tgtccctctt
     4741 ccattccacc atcagcctcg atccgcacct ccgcgattac atctccaggc atttcagccc
     4801 agccaggcca ccactctgcg tgatccatga tgtgttcctc ggctgggttg accaggtggc
     4861 caaggatgtg ggctctacag gcgtggtgtt cacaacaggc ggcgcttatg gcacatccgc
     4921 ctacgtgtcc atctggaacg atctcccgca ccagaactac tccgacgacc aagagttccc
     4981 gctgccaggc ttcccagaga accataagtt ccgcaggtcc cagctccatc ggttcctcag
     5041 atatgccgac ggctccgacg attggtccaa gtatttccag ccgcagctcc gccagtccat
     5101 gaagtctttt ggctggctct gcaactccgt ggaagagatc gagacactcg gcttctccat
     5161 cctccgcaac tacaccaagc tgccgatctg gggcatcggc ccacttattg cttccccagt
     5221 gcagcactcc tcctccgaca acaattcaac aggcgccgag ttcgtgcagt ggctcagcct
     5281 caaagagccg gactccgtcc tctacatctc cttcggctcc cagaacacga tcagcccgac
     5341 gcagatgatg gaactcgctg ctggccttga gtcctccgag aagccattcc tctgggtgat
     5401 cagagccccg ttcggcttcg acatcaacga agagatgcgc ccagagtggc tgccagaggg
     5461 ctttgaggaa cgcatgaagg tgaagaaaca gggcaagctc gtgtacaagc tcggcccgca
     5521 gcttgagatc ctcaaccatg aatccatcgg cggctttctc acccactgcg gatggaacag
     5581 catccttgag tctcttcgcg agggcgttcc gatgcttgga tggccacttg ctgccgagca
     5641 ggcctacaac ctcaagtacc tcgaagatga gatgggcgtc gcggttgagc ttgctagagg
     5701 cctcgaaggc gagatctcca aagagaaggt caagcgcatc gtcgagatga tccttgagcg
     5761 caacgagggc tccaaaggct gggagatgaa gaatcgcgcc gtggaaatgg gcaaaaagct
     5821 caaggacgcc gtgaacgagg aaaaagagct gaagggctcc tccgtgaagg cgatcgacga
     5881 tttcctcgac gccgtcatgc aggccaaact tgagccaagc ctccagtgat agtgagatgt
     5941 gcagatcgtt caaacatttg gcaataaagt ttcttaagat tgaatcctgt tgccggtctt
     6001 gcgatgatta tcatataatt tctgttgaat tacgttaagc atgtaataat taacatgtaa
     6061 tgcatgacgt tatttatgag atgggttttt atgattagag tcccgcaatt atacatttaa
     6121 tacgcgatag aaaacaaaat atagcgcgca aactaggata aattatcgcg cgcggtgtca
     6181 tctatgttac tagatccgat gataagctgt caaacatgag ggacacgaag tggtttaaac
     6241 tatcagtgtt tgacaggata tattggcggg taaacctaag agaaaagagc gtttattaga
     6301 ataatcggat atttaaaagg gcgtgaaaag gtttatccgt tcgtccattt gtatgtgcca
     6361 accacaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     6421 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     6481 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     6541 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     6601 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     6661 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     6721 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     6781 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     6841 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     6901 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     6961 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     7021 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     7081 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     7141 aaacttggtc tgacagctaa aacaattcat ccagtaaaat ataatcgaat attatgcgca
     7201 cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg
     7261 acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt
     7321 gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca
     7381 ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag
     7441 ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc
     7501 cgtgacgatc agcggtccaa tgatcgaagt taggctggta agagccgcga gcgatccttg
     7561 aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat
     7621 cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc
     7681 gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt
     7741 ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat
     7801 tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc
     7861 gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt
     7921 cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa
     7981 ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc
     8041 cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat
     8101 ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct
     8161 catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc
     8221 agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcca
     8281 caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag
     8341 caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa
     8401 ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg
     8461 gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc
     8521 cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg
     8581 actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg
     8641 tcaaacatga gaattcttga aaatattgcg ctgccatttt tggggtgagg ccgttcgcgg
     8701 ccgaggggcg cagcccctgg ggggatggga gctcggtgcc ttgatgtggg cgccggcggt
     8761 cgagtggcga cggcgcggct tgtccgcgcc ctggtagatt gcctggccgt aggccagcca
     8821 tttttgagcg gccagcggcc gcgataggcc gacgcgaagc ggcggggcgt agggagcgca
     8881 gcgaccgaag ggtaggcgct ttttgcagct cttcggctgt gcgctggcca gacagttatg
     8941 cacaggccag gcgggtttta agagttttaa taagttttaa agagttttag gcggaaaaat
     9001 cgcctttttt ctcttttata tcagtcactt acatgtgtga ccggttccca atgtacggct
     9061 ttgggttccc aatgtacggg ttccggttcc caatgtacgg ctttgggttc ccaatgtacg
     9121 tgctatccac aggaaagaag ctttttcgac ctttttcccc tgctagggca atttgcccta
     9181 gcatctgctc cgtacattag gaaccggcgg atgcttcgcc ctcgatcagg ttgcggtagc
     9241 gcatgactag gatcgggcca gcctgccccg cctcctcctt caaatcgtac tccggcaggt
     9301 catttgaccc gatcagcttg cgcacggtga aacagaactt cttgaactct ccggcgctgc
     9361 cactgcgttc gtagatcgtc ttgaacaacc atctggcttc tgccttgcct gcggcgcggc
     9421 gtgccaggcg gtagagaaaa cggccgatgc cgggatcgat caaaaagtaa tcggggtgaa
     9481 ccgtcagcac gtccgggttc ttgccttctg tgatctcgcg gtacatccaa tcagctagct
     9541 cgatctcgat gtactccggc cgcccggttt cgctctttac gatcttgtag cggctaatca
     9601 aggcttcacc ctcggatacc gtcaccaggc ggccgttctt ggccttcttc gtacgctgca
     9661 tggcaacgtg cgtggtgttt aaccgaatgc aggtttctac caggtcgtct ttctgctttc
     9721 cgccatcggc tcgccggcag aacttgagta cgtccgcaac gtgtggacgg aacacgcggc
     9781 cgggcttgtc tcccttccct tcccggtatc ggttcatgga ttcggttaga tgggaaaccg
     9841 ccatcagtac caggtcgtaa tcccacacac tggccatgcc ggccggccct gcggaaacct
     9901 ctacgtgccc gtctggaagc tcgtagcgga tcacctcgcc agctcgtcgg tcacgcttcg
     9961 acagacggaa aacggccacg tccatgatgc tgcgactatc gcgggtgccc acgtcataga
    10021 gcatcggaac gaaaaaatct ggttgctcgt cgcccttggg cggcttccta atcgacgacg
    10081 caccggctgc cggcggttgc cgggattctt tgcggattcg atcagcggcc gcttgccacg
    10141 attcaccggg gcgtgcttct gcctcgatgc gttgccgctg ggcggcctgc gcggccttca
    10201 acttctccac caggtcatca cccagcgccg cgccgatttg taccgggccg gatggtttgc
    10261 gaccgctcac gccgattcct cgggcttggg ggttccagtg ccattgcagg gccggcagac
    10321 aacccagccg cttacgcctg gccaaccgcc cgttcctcca cacatggggc attccacggc
    10381 gtcggtgcct ggttgttctt gattttccat gccgcctcct ttagccgcta aaattcatct
    10441 actcatttat tcatttgctc atttactctg gtagctgcgc gatgtattca gatagcagct
    10501 cggtaatggt cttgccttgg cgtaccgcgt acatcttcag cttggtgtga tcctccgccg
    10561 gcaactgaaa gttgacccgc ttcatggctg gcgtgtctgc caggctggcc aacgttgcag
    10621 ccttgctgct gcgtgcgctc ggacggccgg cacttagcgt gtttgtgctt ttgctcattt
    10681 tctctttacc tcattaactc aaatgagttt tgatttaatt tcagcggcca gcgcctggac
    10741 ctcgcgggca gcgtcgccct cgggttctga ttcaagaacg gttgtgccgg cggcggcagt
    10801 gcctgggtag ctcacgcgct gcgtgatacg ggactcaaga atgggcagct cgtacccggc
    10861 cagcgcctcg gcaacctcac cgccgatgcg cgtgcctttg atcgcccgcg acacgacaaa
    10921 ggccgcttgt agccttccat ccgtgacctc aatgcgctgc ttaaccagct ccaccaggtc
    10981 ggcggtggcc catatgtcgt aagggcttgg ctgcaccgga atcagcacga agtcggctgc
    11041 cttgatcgcg gacacagcca agtccgccgc ctggggcgct ccgtcgatca ctacgaagtc
    11101 gcgccggccg atggccttca cgtcgcggtc aatcgtcggg cggtcgatgc cgacaacggt
    11161 tagcggttga tcttcccgca cggccgccca atcgcgggca ctgccctggg gatcggaatc
    11221 gactaacaga acatcggccc cggcgagttg cagggcgcgg gctagatggg ttgcgatggt
    11281 cgtcttgcct gacccgcctt tctggttaag tacagcgata accttcatgc gttccccttg
    11341 cgtatttgtt tatttactca tcgcatcata tacgcagcga ccgcatgacg caagctgttt
    11401 tactcaaata cacatcacct ttttagacgg cggcgctcgg tttcttcagc ggccaagctg
    11461 gccggccagg ccgccagctt ggcatcagac aaaccggcca ggatttcatg cagccgcacg
    11521 gctgatgggc tgcctgtatc gagtggtgat tttgtgccga gctgccggtc ggggagctgt
    11581 tggctggctg gtggcaggat atattgtggt gtaaacaaat tgacgcttag acaacttaat
    11641 aacacattgc ggacgttttt aatgtactgg ggtggatgca gtgggcccca ctctgtggaa
    11701 gacaatgcct ccggagtaac agataaagcg aagcttgaag gtgcatgtga ctccgtcaag
    11761 attacgaaac cgccaactac cacgcaaatt gcaattctca atttcctaga aggactctcc
    11821 gaaaatgcat ccaataccaa atattacccg tgtcataggc accaagtgac accatacatg
    11881 aacacgcgtc acaatatgac tggagaaggg ttccacacct tatgctataa aacgccccac
    11941 acccctcctc cttccttcgc agttcaattc caatatattc cattctctct gtgtatttcc
    12001 ctacctctcc cttcaaggtt agtcgatttc ttctgttttt cttcttcgtt ctttccatga
    12061 attgtgtatg ttctttgatc aatacgatgt tgatttgatt gtgttttgtt tggtttcatc
    12121 gatcttcaat tttcataatc agattcagct tttattatct ttacaacaac gtccttaatt
    12181 tgatgattct ttaatcgtag atttgctcta attagagctt tttcatgtca gatcccttta
    12241 caacaagcct taattgttga ttcattaatc gtagattagg gcttttttca ttgattactt
    12301 cagatccgtt aaacgtaacc atagatcagg gctttttcat gaattacttc agatccgtta
    12361 aacaacagcc ttatttttta tacttctgtg gtttttcaag aaattgttca gatccgttga
    12421 caaaaagcct tattcgttga ttctatatcg tttttcgaga gatattgctc agatctgtta
    12481 gcaactgcct tgtttgttga ttctattgcc gtggattagg gttttttttc acgagattgc
    12541 ttcagatccg tacttaagat tacgtaatgg attttgattc tgatttatct gtgattgttg
    12601 actcgacagc ggccgcaccg gcgcgccatg gcagatccaa tgaagctact gtcttctatc
    12661 gaacaagcat gcgatatttg ccgacttaaa aagctcaagt gctccaaaga aaaaccgaag
    12721 tgcgccaagt gtctgaagaa caactgggag tgtcgctact ctcccaaaac caaaagatct
    12781 ccgctgacta gggcacatct gacagaagtg gaatcaaggc tagaaagact ggaacagcta
    12841 tttctactga tttttccaag gtcgagcgcc cccccgaccg atgtcagcct gggggacgag
    12901 ctccacttag acggcgagga cgtggcgatg gcgcatgccg acgcgctaga cgatttcgat
    12961 ctggacatgt tgggggacgg ggattccccg ggtccgggat ttacccccca cgactccgcc
    13021 ccctacggcg ctctggatat ggccgacttc gagtttgagc agatgtttac cgatgccctt
    13081 ggaattgacg agtacggtgg ggatccaatt cagcaagcca ctgcaggagt ctcacaagac
    13141 acttcggaaa atcctaacaa aacaatagtt cctgctgcat taccacagct cacccctacc
    13201 ttggtgtcac tgctggaggt gattgaaccc gaggtgttgt atgcaggata tgatagctct
    13261 gttccagatt cagcatggag aattatgacc acactcaaca tgttaggtgg gcgtcaagtg
    13321 attgcagcag tgaaatgggc aaaggcgata ccaggcttca gaaacttaca cctggatgac
    13381 caaatgaccc tgctacagta ctcatggatg tttctcatgg catttgccct gggttggaga
    13441 tcatacagac aatcaagtgg aaacctgctc tgctttgctc ctgatctgat tattaatgag
    13501 cagagaatgt ctctaccctg catgtatgac caatgtaaac acatgctgtt tgtctcctct
    13561 gaattacaaa gattgcaggt atcctatgaa gagtatctct gtatgaaaac cttactgctt
    13621 ctctcctcag ttcctaagga aggtctgaag agccaagagt tatttgatga gattcgaatg
    13681 acttatatca aagagctagg aaaagccatc gtcaaaaggg aagggaactc cagtcagaac
    13741 tggcaacggt tttaccaact gacaaagctt ctggactcca tgcatgaggt ggttgagaat
    13801 ctccttacct actgcttcca gacatttttg gataagacca tgagtattga attcccagag
    13861 atgttagctg aaatcatcac taatcagata ccaaaatatt caaatggaaa tatcaaaaag
    13921 cttctgtttc atcaaaaatg actcgaccta actgagtaag ctagcttgtt cgagtattat
    13981 ggcattggga aaactgtttt tcttgtacca tttgttgtgc ttgtaattta ctgtgttttt
    14041 tattcggttt tcgctatcga actgtgaaat ggaaatggat ggagaagagt taatgaatga
    14101 tatggtcctt ttgttcattc tcaaattaat attatttgtt ttttctctta tttgttgtgt
    14161 gttgaatttg aaattataag agatatgcaa acattttgtt ttgagtaaaa atgtgtcaaa
    14221 tcgtggcctc taatgaccga agttaatatg aggagtaaaa cactagatcc ccaaacaagc
    14281 ttgaaactga aggcgggaaa cgacaatctg atcatgagcg gagaattaag ggagtcacgt
    14341 tatgaccccc gccgatgacg cgggacaagc cgttttacgt ttggaactga catatgaaaa
    14401 tattttttaa tgcattttat gacttgccaa ttgattgaca acatgcatca atccgcggtt
    14461 atgactctct taagagagtc atgaattcga gcgggaattg atcccccctc gacagcttgc
    14521 atgccggtcg actctagagg atccgggtga cagccctccg acgggtgaca gccctccgac
    14581 gggtgacagc cctccgaatt ctagaggatc cgggtgacag ccctccgacg ggtgacagcc
    14641 ctccgacggg tgacagccct ccgaattcga gctcagtacc cggggatctg tcgacctcga
    14701 tcgagatctt cgcaagaccc ttcctctata taaggaagtt catttcattt ggagaggaca
    14761 cgctgaagct agtcgactct agcctcgaat ccttaacaaa aatggcaagg gaccccccag
    14821 tcgcgaccat gattcctctg ttaaacgcaa agcagattct cgggcagttg gatgggggga
    14881 gtgtggcggt cctgccagta gtcgatagta ctaatcaata tctgctagat agaataggtg
    14941 agttaaaaag cggagacgcg tgcatagccg aatatcaaca agcgggaaga ggctcacgag
    15001 gaaggaagtg gtttagccca ttcggggcga atctgtatct ttctatgttc tggcgtctga
    15061 agagaggccc ggcagcgatt ggattaggcc cggtcatagg catcgttatg gctgaggctt
    15121 taagaaagct aggtgctgac aaagtgcgag tgaagtggcc aaatgactta tacttgcaag
    15181 acaggaagct agcagggatt ttagtcgagc tagccggtat cacaggtgac gctgcacaaa
    15241 ttgttatagg ggccggtatc aatgtggcca tgcgtagagt ggaagaaagc gtcgtgaacc
    15301 aaggatggat cactctacag gaggctggaa ttaatttgga tcgaaacacc ttagctgcga
    15361 tgctaattag agaactgcgt gcggcactgg agctgttcga gcaggaaggc ttggccccct
    15421 acctatcaag gtgggaaaag ctggataact tcattaaccg tccagtgaaa ttgattattg
    15481 gagataagga aatctttggc atctcacgag ggattgacaa gcaaggggca ttactgcttg
    15541 aacaggatgg ggttattaag ccttggatgg gaggggaaat ctccttaagg tcagcggaga
    15601 aggcgtccgg gaagccaatc ccaaatcccc ttctcggtct tgacagtacc ggaggtggtt
    15661 ctggtaccac aagtttgtac aaaaaagctg aacgagaaac gtaaaatgat ataaatatca
    15721 atatattaaa ttagattttg cataaaaaac agactacata atactgtaaa acacaacata
    15781 tccagtcact atggcggccg cattaggcac cccaggcttt acactttatg cttccggctc
    15841 gtataatgtg tggattttga gttaggatcc gtcgagattt tcaggagcta aggaagctaa
    15901 aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga
    15961 acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga
    16021 tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat
    16081 tcacattctt gcccgcctga tgaatgctca tccggaattc cgtatggcaa tgaaagacgg
    16141 tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg agca
//
