LOCUS       sequence-464380-       13895 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GATEWAY compatible vector with a soybean Ubiquitin (GmUbi) promoter 
            driving in frame epitope fusion of eGFP paired with ccdb selection 
            toxin and RUBY marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13895)
  TITLE     GATEWAY compatible vector with a promoter driving in frame epitope 
            fusion paired with ccdb selection toxin and FP marker
REFERENCE   2  (bases 1 to 13895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Ubi:eGFP-ccdb + RUBY
FEATURES             Location/Qualifiers
     source          1..13895
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(879..1508)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    1772..1796
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             join(2830..2832,2833..2835,2836..3546)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2830..2832,2833..2835,2836..3546)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2875..2896)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3136..3155)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3483..3504
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    3553..3677
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(3702..3707,3708..3725,3726..3732)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             3786..4445
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(3852..3871)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             4787..5092
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     4971..4990
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(5133..5257)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             6054..10004
                     /label=RUBY
     CDS             7554..7610
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             8445..8501
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     terminator      10019..10271
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    10326..10350
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(10473..11061)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10553..10572)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(11453..12643)
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     12272..12291
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        complement(join(12691..12696,12697..12713,12714..12719))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     rep_origin      13012..13206
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(join(13272..13895,1..450))
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
ORIGIN
        1 ttcggttaga tgggaaaccg ccatcagtac caggtcgtaa tcccacacac tggccatgcc
       61 ggccggccct gcggaaacct ctacgtgccc gtctggaagc tcgtagcgga tcacctcgcc
      121 agctcgtcgg tcacgcttcg acagacggaa aacggccacg tccatgatgc tgcgactatc
      181 gcgggtgccc acgtcataga gcatcggaac gaaaaaatct ggttgctcgt cgcccttggg
      241 cggcttccta atcgacgacg caccggctgc cggcggttgc cgggattctt tgcggattcg
      301 atcagcggcc gcttgccacg attcaccggg gcgtgcttct gcctcgatgc gttgccgctg
      361 ggcggcctgc gcggccttca acttctccac caggtcatca cccagcgccg cgccgatttg
      421 taccgggccg gatggtttgc gaccgctcac gccgattcct cgggcttggg ggttccagtg
      481 ccattgcagg gccggcagac aacccagccg cttacgcctg gccaaccgcc cgttcctcca
      541 cacatggggc attccacggc gtcggtgcct ggttgttctt gattttccat gccgcctcct
      601 ttagccgcta aaattcatct actcatttat tcatttgctc atttactctg gtagctgcgc
      661 gatgtattca gatagcagct cggtaatggt cttgccttgg cgtaccgcgt acatcttcag
      721 cttggtgtga tcctccgccg gcaactgaaa gttgacccgc ttcatggctg gcgtgtctgc
      781 caggctggcc aacgttgcag ccttgctgct gcgtgcgctc ggacggccgg cacttagcgt
      841 gtttgtgctt ttgctcattt tctctttacc tcattaactc aaatgagttt tgatttaatt
      901 tcagcggcca gcgcctggac ctcgcgggca gcgtcgccct cgggttctga ttcaagaacg
      961 gttgtgccgg cggcggcagt gcctgggtag ctcacgcgct gcgtgatacg ggactcaaga
     1021 atgggcagct cgtacccggc cagcgcctcg gcaacctcac cgccgatgcg cgtgcctttg
     1081 atcgcccgcg acacgacaaa ggccgcttgt agccttccat ccgtgacctc aatgcgctgc
     1141 ttaaccagct ccaccaggtc ggcggtggcc catatgtcgt aagggcttgg ctgcaccgga
     1201 atcagcacga agtcggctgc cttgatcgcg gacacagcca agtccgccgc ctggggcgct
     1261 ccgtcgatca ctacgaagtc gcgccggccg atggccttca cgtcgcggtc aatcgtcggg
     1321 cggtcgatgc cgacaacggt tagcggttga tcttcccgca cggccgccca atcgcgggca
     1381 ctgccctggg gatcggaatc gactaacaga acatcggccc cggcgagttg cagggcgcgg
     1441 gctagatggg ttgcgatggt cgtcttgcct gacccgcctt tctggttaag tacagcgata
     1501 accttcatgc gttccccttg cgtatttgtt tatttactca tcgcatcata tacgcagcga
     1561 ccgcatgacg caagctgttt tactcaaata cacatcacct ttttagacgg cggcgctcgg
     1621 tttcttcagc ggccaagctg gccggccagg ccgccagctt ggcatcagac aaaccggcca
     1681 ggatttcatg cagccgcacg gctgatgggc tgcctgtatc gagtggtgat tttgtgccga
     1741 gctgccggtc ggggagctgt tggctggctg gtggcaggat atattgtggt gtaaacaaat
     1801 tgacgcttag acaacttaat aacacattgc ggacgttttt aatgtactgg ggtggatgca
     1861 gtgggcccca ctctgtggaa gacaatgccc cgcggtccgg agtaacagat aaagcgaagc
     1921 ttgaaggtgc atgtgactcc gtcaagatta cgaaaccgcc aactaccacg caaattgcaa
     1981 ttctcaattt cctagaagga ctctccgaaa atgcatccaa taccaaatat tacccgtgtc
     2041 ataggcacca agtgacacca tacatgaaca cgcgtcacaa tatgactgga gaagggttcc
     2101 acaccttatg ctataaaacg ccccacaccc ctcctccttc cttcgcagtt caattccaat
     2161 atattccatt ctctctgtgt atttccctac ctctcccttc aaggttagtc gatttcttct
     2221 gtttttcttc ttcgttcttt ccatgaattg tgtatgttct ttgatcaata cgatgttgat
     2281 ttgattgtgt tttgtttggt ttcatcgatc ttcaattttc ataatcagat tcagctttta
     2341 ttatctttac aacaacgtcc ttaatttgat gattctttaa tcgtagattt gctctaatta
     2401 gagctttttc atgtcagatc cctttacaac aagccttaat tgttgattca ttaatcgtag
     2461 attagggctt ttttcattga ttacttcaga tccgttaaac gtaaccatag atcagggctt
     2521 tttcatgaat tacttcagat ccgttaaaca acagccttat tttttatact tctgtggttt
     2581 ttcaagaaat tgttcagatc cgttgacaaa aagccttatt cgttgattct atatcgtttt
     2641 tcgagagata ttgctcagat ctgttagcaa ctgccttgtt tgttgattct attgccgtgg
     2701 attagggttt tttttcacga gattgcttca gatccgtact taagattacg taatggattt
     2761 tgattctgat ttatctgtga ttgttgactc gacagcggcc gcacccccgg gggcgcgccc
     2821 ttaggtacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     2881 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     2941 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     3001 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac
     3061 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     3121 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     3181 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     3241 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
     3301 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
     3361 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     3421 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
     3481 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     3541 tacaaggata tcacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa tgatataaat
     3601 atcaatatat taaattagat tttgcataaa aaacagacta cataatactg taaaacacaa
     3661 catatccagt cactatggcg gccgcattag gcaccccagg ctttacactt tatgcttccg
     3721 gctcgtataa tgtgtggatt ttgagttagg atccgtcgag attttcagga gctaaggaag
     3781 ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa tggcatcgta
     3841 aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag accgttcagc
     3901 tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt tatccggcct
     3961 ttattcacat tcttgcccgc ctgatgaatg ctcatccgga attccgtatg gcaatgaaag
     4021 acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc catgagcaaa
     4081 ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag tttctacaca
     4141 tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct aaagggttta
     4201 ttgagaatat gtttttcgtc tcagccaatc cctgggtgag tttcaccagt tttgatttaa
     4261 acgtggccaa tatggacaac ttcttcgccc ccgttttcac catgggcaaa tattatacgc
     4321 aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt tgtgatggct
     4381 tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg cagggcgggg
     4441 cgtaaacgcg tggatccggc ttactaaaag ccagataaca gtatgcgtat ttgcgcgctg
     4501 atttttgcgg tataagaata tatactgata tgtatacccg aagtatgtca aaaagaggta
     4561 tgctatgaag cagcgtatta cagtgacagt tgacagcgac agctatcagt tgctcaaggc
     4621 atatatgatg tcaatatctc cggtctggta agcacaacca tgcagaatga agcccgtcgt
     4681 ctgcgtgccg aacgctggaa agcggaaaat caggaaggga tggctgaggt cgcccggttt
     4741 attgaaatga acggctcttt tgctgacgag aacaggggct ggtgaaatgc agtttaaggt
     4801 ttacacctat aaaagagaga gccgttatcg tctgtttgtg gatgtacaga gtgatattat
     4861 tgacacgccc gggcgacgga tggtgatccc cctggccagt gcacgtctgc tgtcagataa
     4921 agtctcccgt gaactttacc cggtggtgca tatcggggat gaaagctggc gcatgatgac
     4981 caccgatatg gccagtgtgc cggtctccgt tatcggggaa gaagtggctg atctcagcca
     5041 ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc tggggaatat aaatgtcagg
     5101 ctcccttata cacagccagt ctgcaggtcg accatagtga ctggatatgt tgtgttttac
     5161 agtattatgt agtctgtttt ttatgcaaaa tctaatttaa tatattgata tttatatcat
     5221 tttacgtttc tcgttcagct ttcttgtaca aagtggtcct aggttcggtt cgagtattat
     5281 ggcattggga aaactgtttt tcttgtacca tttgttgtgc ttgtaattta ctgtgttttt
     5341 tattcggttt tcgctatcga actgtgaaat ggaaatggat ggagaagagt taatgaatga
     5401 tatggtcctt ttgttcattc tcaaattaat attatttgtt ttttctctta tttgttgtgt
     5461 gttgaatttg aaattataag agatatgcaa acattttgtt ttgagtaaaa atgtgtcaaa
     5521 tcaaggttcc agaaggtaat tatccaagat gtagcatcaa gaatccaatg tttacgggaa
     5581 aaactatgga agtattatgt gagctcagca agaagcagat caatatgcgg cacatatgca
     5641 acctatgttc aaaaatgaag aatgtacaga tacaagatcc tatactgcca gaatacgaag
     5701 aagaatacgt agaaattgaa aaagaagaac caggcgaaga aaagaatctt gaagacgtaa
     5761 gcactgacga caacaatgaa aagaagaaga taaggtcggt gattgtgaaa gagacataga
     5821 ggacacatgt aaggtggaaa atgtaagggc ggaaagtaac cttatcacaa aggaatctta
     5881 tcccccacta cttatccttt tatatttttc cgtgtcattt ttgcccttga gttttcctat
     5941 ataaggaacc aagttcggca tttgtgaaaa caagaaaaaa tttggtgtaa gctattttct
     6001 ttgaagtact gaggatacaa cttcagagaa atttgtaagt ttgtgatccg caaatggatc
     6061 atgcgaccct cgccatgatc ctcgcgatct ggttcatcag cttccacttc atcaagctgc
     6121 tgttctccca gcagaccacc aagctgcttc cgccaggacc aaagccgctt ccgatcatcg
     6181 gcaacatcct tgaggtgggc aagaagccgc atcggtcctt cgccaacctc gccaagattc
     6241 acggcccact catttccctc agactcggct ctgtgaccac catcgttgtg tcctctgccg
     6301 acgtggccaa agagatgttc ctcaagaagg atcacccgct ctccaaccgc acgatcccga
     6361 atagtgttac agccggcgac caccacaagc tcaccatgtc ttggctcccg gtgtctccga
     6421 agtggcgcaa cttccgcaag attaccgccg tgcatctgct ctccccacag agactcgatg
     6481 cctgccagac attcaggcac gccaaggtgc agcagctcta cgagtacgtt caagagtgcg
     6541 cccagaaagg ccaggccgtg gatattggca aggccgcctt tacgaccagc ctcaacctcc
     6601 tcagcaagct gttcttcagc gtcgagctgg cgcaccacaa gtcccatacc agccaagagt
     6661 tcaaagagct gatctggaac atcatggaag atataggcaa gccgaactac gccgactact
     6721 tcccgattct cggctgcgtt gacccatctg gcattagaag aaggctcgcc tgctccttcg
     6781 acaagctgat cgccgtgttc cagggcatca tctgcgagag actcgcccca gattcctcca
     6841 ccacaactac caccaccacc gacgacgtgc tcgatgtgct cctccagctg ttcaagcaga
     6901 acgagctgac gatgggcgag atcaaccacc tcctcgtgga catcttcgac gccggcaccg
     6961 ataccacatc ctccacattc gagtgggtga tgaccgagct gatccgcaat ccagagatga
     7021 tggaaaaggc ccaagaggaa atcaagcagg tcctcggcaa ggacaagcag atccaagagt
     7081 ccgacatcat caacctgccg tacctccagg cgatcatcaa agagacactc cgcctccatc
     7141 cgccgaccgt gttcttgctc ccaagaaagg ccgacaccga tgtcgagctg tacggctaca
     7201 tcgtgccgaa ggatgcccag atcctcgtga acctctgggc cattggcagg gacccaaacg
     7261 cctggcagaa cgccgatatt ttcagcccag agcgcttcat cggctgcgag atcgatgtta
     7321 agggccgcga tttcggcctc cttccatttg gcgctggccg cagaatttgc ccaggcatga
     7381 atctcgccat caggatgctc accctcatgc tcgccacact cctccagttc ttcaactgga
     7441 agctcgaagg cgacatctcc ccgaaggacc tcgacatgga cgagaagttc ggcattgcgc
     7501 tccaaaagac caagccgctc aagctcatcc cgattccgcg ctacggtagc ggagctacca
     7561 attttagcct ccttaagcag gcaggtgatg tagaagagaa ccccgggcct atgaagatga
     7621 tgaacggcga ggacgccaac gaccagatga tcaaagagtc cttcttcatc acccacggca
     7681 acccgatcct caccgtcgag gatacacatc cgctcaggcc gttcttcgag acatggcgcg
     7741 agaagatttt ctccaagaag ccgaaggcca tcctcatcat ctccggccac tgggagacag
     7801 tgaagccaac cgtgaacgcc gtgcacatca acgacaccat ccacgacttc gacgactacc
     7861 cagccgccat gtaccagttc aagtacccag ctccaggcga gccagagctt gcgagaaagg
     7921 tggaagagat cctcaagaag tccgggttcg agacagccga gacagaccaa aagaggggcc
     7981 ttgatcacgg cgcctgggtt ccactcatgc tcatgtatcc agaggcggac atcccggtgt
     8041 gccagctctc agttcagcca catctcgacg gcacctacca ctacaatctc ggcagagccc
     8101 tcgcgccgct caagaatgat ggcgtgctca ttattggctc cggcagcgcc acacatccac
     8161 tcgatgagac accgcactac ttcgatggtg ttgccccttg ggccgctgcc ttcgattctt
     8221 ggcttaggaa ggccctcatc aacggccgct tcgaggaagt gaacatctac gagagcaagg
     8281 ccccgaactg gaagctcgcc catccatttc cagagcactt ctacccgctc cacgttgtgc
     8341 tcggcgctgc tggtgaaaag tggaaggccg agctgatcca ctcctcctgg gatcatggca
     8401 cactttgcca cggctcctac aagttcacct ccgccggatc cggagcaacc aactttagcc
     8461 tgctcaagca agcaggagat gttgaggaaa atcctggccc catgaccgcc atcaagatga
     8521 acaccaacgg cgagggcgag acacagcaca tcctcatgat cccgttcatg gcgcagggcc
     8581 acctcaggcc atttctcgaa ctcgccatgt tcctctacaa gcgctcccac gtgatcatca
     8641 ccctgctcac aactccgctc aatgccggct tcctcaggca cctccttcac caccattcct
     8701 actcctccag cggcatcagg atcgtcgagc tgccattcaa ctccaccaac cacggactcc
     8761 caccgggcat cgagaacacc gataagctca cactcccgct cgtggtgtcc ctcttccatt
     8821 ccaccatcag cctcgatccg cacctccgcg attacatctc caggcatttc agcccagcca
     8881 ggccaccact ctgcgtgatc catgatgtgt tcctcggctg ggttgaccag gtggccaagg
     8941 atgtgggctc tacaggcgtg gtgttcacaa caggcggcgc ttatggcaca tccgcctacg
     9001 tgtccatctg gaacgatctc ccgcaccaga actactccga cgaccaagag ttcccgctgc
     9061 caggcttccc agagaaccat aagttccgca ggtcccagct ccatcggttc ctcagatatg
     9121 ccgacggctc cgacgattgg tccaagtatt tccagccgca gctccgccag tccatgaagt
     9181 cttttggctg gctctgcaac tccgtggaag agatcgagac actcggcttc tccatcctcc
     9241 gcaactacac caagctgccg atctggggca tcggcccact tattgcttcc ccagtgcagc
     9301 actcctcctc cgacaacaat tcaacaggcg ccgagttcgt gcagtggctc agcctcaaag
     9361 agccggactc cgtcctctac atctccttcg gctcccagaa cacgatcagc ccgacgcaga
     9421 tgatggaact cgctgctggc cttgagtcct ccgagaagcc attcctctgg gtgatcagag
     9481 ccccgttcgg cttcgacatc aacgaagaga tgcgcccaga gtggctgcca gagggctttg
     9541 aggaacgcat gaaggtgaag aaacagggca agctcgtgta caagctcggc ccgcagcttg
     9601 agatcctcaa ccatgaatcc atcggcggct ttctcaccca ctgcggatgg aacagcatcc
     9661 ttgagtctct tcgcgagggc gttccgatgc ttggatggcc acttgctgcc gagcaggcct
     9721 acaacctcaa gtacctcgaa gatgagatgg gcgtcgcggt tgagcttgct agaggcctcg
     9781 aaggcgagat ctccaaagag aaggtcaagc gcatcgtcga gatgatcctt gagcgcaacg
     9841 agggctccaa aggctgggag atgaagaatc gcgccgtgga aatgggcaaa aagctcaagg
     9901 acgccgtgaa cgaggaaaaa gagctgaagg gctcctccgt gaaggcgatc gacgatttcc
     9961 tcgacgccgt catgcaggcc aaacttgagc caagcctcca gtgatagtga gatgtgcaga
    10021 tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg gtcttgcgat
    10081 gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca tgtaatgcat
    10141 gacgttattt atgagatggg tttttatgat tagagtcccg caattataca tttaatacgc
    10201 gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg tgtcatctat
    10261 gttactagat ccgatgataa gctgtcaaac atgagggaca cgaagtggtt taaactatca
    10321 gtgtttgaca ggatatattg gcgggtaaac ctaagagaaa agagcgttta ttagaataat
    10381 cggatattta aaagggcgtg aaaaggttta tccgttcgtc catttgtatg tgccaaccac
    10441 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
    10501 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
    10561 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
    10621 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
    10681 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
    10741 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
    10801 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
    10861 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
    10921 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
    10981 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
    11041 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
    11101 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
    11161 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
    11221 tggtctgaca gctaaaacaa ttcatccagt aaaatataat cgaatattat gcgcacccgt
    11281 ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg
    11341 atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg
    11401 gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg
    11461 aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg
    11521 cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga
    11581 cgatcagcgg tccaatgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct
    11641 gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga
    11701 tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg
    11761 ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc
    11821 cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga
    11881 ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa
    11941 tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa
    12001 gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc
    12061 tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta
    12121 gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc
    12181 ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga
    12241 gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa
    12301 ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atccacagga
    12361 cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga
    12421 ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca
    12481 tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga
    12541 tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg
    12601 tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc
    12661 gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa
    12721 catgagaatt cttgaaaata ttgcgctgcc atttttgggg tgaggccgtt cgcggccgag
    12781 gggcgcagcc cctgggggga tgggagctcg gtgccttgat gtgggcgccg gcggtcgagt
    12841 ggcgacggcg cggcttgtcc gcgccctggt agattgcctg gccgtaggcc agccattttt
    12901 gagcggccag cggccgcgat aggccgacgc gaagcggcgg ggcgtaggga gcgcagcgac
    12961 cgaagggtag gcgctttttg cagctcttcg gctgtgcgct ggccagacag ttatgcacag
    13021 gccaggcggg ttttaagagt tttaataagt tttaaagagt tttaggcgga aaaatcgcct
    13081 tttttctctt ttatatcagt cacttacatg tgtgaccggt tcccaatgta cggctttggg
    13141 ttcccaatgt acgggttccg gttcccaatg tacggctttg ggttcccaat gtacgtgcta
    13201 tccacaggaa agaagctttt tcgacctttt tcccctgcta gggcaatttg ccctagcatc
    13261 tgctccgtac attaggaacc ggcggatgct tcgccctcga tcaggttgcg gtagcgcatg
    13321 actaggatcg ggccagcctg ccccgcctcc tccttcaaat cgtactccgg caggtcattt
    13381 gacccgatca gcttgcgcac ggtgaaacag aacttcttga actctccggc gctgccactg
    13441 cgttcgtaga tcgtcttgaa caaccatctg gcttctgcct tgcctgcggc gcggcgtgcc
    13501 aggcggtaga gaaaacggcc gatgccggga tcgatcaaaa agtaatcggg gtgaaccgtc
    13561 agcacgtccg ggttcttgcc ttctgtgatc tcgcggtaca tccaatcagc tagctcgatc
    13621 tcgatgtact ccggccgccc ggtttcgctc tttacgatct tgtagcggct aatcaaggct
    13681 tcaccctcgg ataccgtcac caggcggccg ttcttggcct tcttcgtacg ctgcatggca
    13741 acgtgcgtgg tgtttaaccg aatgcaggtt tctaccaggt cgtctttctg ctttccgcca
    13801 tcggctcgcc ggcagaactt gagtacgtcc gcaacgtgtg gacggaacac gcggccgggc
    13861 ttgtctccct tcccttcccg gtatcggttc atgga
//
