LOCUS       sequence-464382-       10802 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GATEWAY compatible vector with a soybean Ubiquitin (GmUbi) promoter 
            driving in frame epitope fusion of miniTurbo-ID paired with ccdb 
            selection toxin and mCherry marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10802)
  TITLE     GATEWAY compatible vector with a promoter driving in frame epitope 
            fusion paired with ccdb selection toxin and FP marker
REFERENCE   2  (bases 1 to 10802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Ubi:mTb-ccdb + mcherry
FEATURES             Location/Qualifiers
     source          1..10802
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             709..1014
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     893..912
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1055..1179)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1985..2695
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     2398..2417
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     terminator      2704..2956
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    3011..3035
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(3158..3746)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3238..3257)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(4138..5328)
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     4957..4976
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        complement(join(5376..5381,5382..5398,5399..5404))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     rep_origin      5697..5891
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(5957..7030)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(7459..8088)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    8352..8376
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             10217..10258
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    10277..10401
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(10426..10431,10432..10449,10450..10456)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             join(10510..10802,1..367)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(10576..10595)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 aactgaaacg ttttcatcgc tctggagtga ataccacgac gatttccggc agtttctaca
       61 catatattcg caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt
      121 tattgagaat atgtttttcg tctcagccaa tccctgggtg agtttcacca gttttgattt
      181 aaacgtggcc aatatggaca acttcttcgc ccccgttttc accatgggca aatattatac
      241 gcaaggcgac aaggtgctga tgccgctggc gattcaggtt catcatgccg tttgtgatgg
      301 cttccatgtc ggcagaatgc ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg
      361 ggcgtaaacg cgtggatccg gcttactaaa agccagataa cagtatgcgt atttgcgcgc
      421 tgatttttgc ggtataagaa tatatactga tatgtatacc cgaagtatgt caaaaagagg
      481 tatgctatga agcagcgtat tacagtgaca gttgacagcg acagctatca gttgctcaag
      541 gcatatatga tgtcaatatc tccggtctgg taagcacaac catgcagaat gaagcccgtc
      601 gtctgcgtgc cgaacgctgg aaagcggaaa atcaggaagg gatggctgag gtcgcccggt
      661 ttattgaaat gaacggctct tttgctgacg agaacagggg ctggtgaaat gcagtttaag
      721 gtttacacct ataaaagaga gagccgttat cgtctgtttg tggatgtaca gagtgatatt
      781 attgacacgc ccgggcgacg gatggtgatc cccctggcca gtgcacgtct gctgtcagat
      841 aaagtctccc gtgaacttta cccggtggtg catatcgggg atgaaagctg gcgcatgatg
      901 accaccgata tggccagtgt gccggtctcc gttatcgggg aagaagtggc tgatctcagc
      961 caccgcgaaa atgacatcaa aaacgccatt aacctgatgt tctggggaat ataaatgtca
     1021 ggctccctta tacacagcca gtctgcaggt cgaccatagt gactggatat gttgtgtttt
     1081 acagtattat gtagtctgtt ttttatgcaa aatctaattt aatatattga tatttatatc
     1141 attttacgtt tctcgttcag ctttcttgta caaagtggtc ctaggtgatt cggttcgagt
     1201 attatggcat tgggaaaact gtttttcttg taccatttgt tgtgcttgta atttactgtg
     1261 ttttttattc ggttttcgct atcgaactgt gaaatggaaa tggatggaga agagttaatg
     1321 aatgatatgg tccttttgtt cattctcaaa ttaatattat ttgttttttc tcttatttgt
     1381 tgtgtgttga atttgaaatt ataagagata tgcaaacatt ttgttttgag taaaaatgtg
     1441 tcaaatcaag gttccagaag gtaattatcc aagatgtagc atcaagaatc caatgtttac
     1501 gggaaaaact atggaagtat tatgtgagct cagcaagaag cagatcaata tgcggcacat
     1561 atgcaaccta tgttcaaaaa tgaagaatgt acagatacaa gatcctatac tgccagaata
     1621 cgaagaagaa tacgtagaaa ttgaaaaaga agaaccaggc gaagaaaaga atcttgaaga
     1681 cgtaagcact gacgacaaca atgaaaagaa gaagataagg tcggtgattg tgaaagagac
     1741 atagaggaca catgtaaggt ggaaaatgta agggcggaaa gtaaccttat cacaaaggaa
     1801 tcttatcccc cactacttat ccttttatat ttttccgtgt catttttgcc cttgagtttt
     1861 cctatataag gaaccaagtt cggcatttgt gaaaacaaga aaaaatttgg tgtaagctat
     1921 tttctttgaa gtactgagga tacaacttca gagaaatttg taagtttgtg atccgcaaac
     1981 aaaaatggtg agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt
     2041 caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga
     2101 gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct
     2161 gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa
     2221 gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga
     2281 gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca
     2341 ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc
     2401 cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga
     2461 cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga
     2521 cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa
     2581 cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta
     2641 cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca agtgagatgt
     2701 gcagatcgtt caaacatttg gcaataaagt ttcttaagat tgaatcctgt tgccggtctt
     2761 gcgatgatta tcatataatt tctgttgaat tacgttaagc atgtaataat taacatgtaa
     2821 tgcatgacgt tatttatgag atgggttttt atgattagag tcccgcaatt atacatttaa
     2881 tacgcgatag aaaacaaaat atagcgcgca aactaggata aattatcgcg cgcggtgtca
     2941 tctatgttac tagatccgat gataagctgt caaacatgag ggacacgaag tggtttaaac
     3001 tatcagtgtt tgacaggata tattggcggg taaacctaag agaaaagagc gtttattaga
     3061 ataatcggat atttaaaagg gcgtgaaaag gtttatccgt tcgtccattt gtatgtgcca
     3121 accacaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     3181 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     3241 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     3301 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     3361 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     3421 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     3481 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     3541 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     3601 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     3661 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     3721 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     3781 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     3841 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     3901 aaacttggtc tgacagctaa aacaattcat ccagtaaaat ataatcgaat attatgcgca
     3961 cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg
     4021 acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt
     4081 gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca
     4141 ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag
     4201 ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc
     4261 cgtgacgatc agcggtccaa tgatcgaagt taggctggta agagccgcga gcgatccttg
     4321 aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat
     4381 cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc
     4441 gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt
     4501 ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat
     4561 tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc
     4621 gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt
     4681 cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa
     4741 ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc
     4801 cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat
     4861 ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct
     4921 catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc
     4981 agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcca
     5041 caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag
     5101 caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa
     5161 ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg
     5221 gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc
     5281 cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg
     5341 actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg
     5401 tcaaacatga gaattcttga aaatattgcg ctgccatttt tggggtgagg ccgttcgcgg
     5461 ccgaggggcg cagcccctgg ggggatggga gctcggtgcc ttgatgtggg cgccggcggt
     5521 cgagtggcga cggcgcggct tgtccgcgcc ctggtagatt gcctggccgt aggccagcca
     5581 tttttgagcg gccagcggcc gcgataggcc gacgcgaagc ggcggggcgt agggagcgca
     5641 gcgaccgaag ggtaggcgct ttttgcagct cttcggctgt gcgctggcca gacagttatg
     5701 cacaggccag gcgggtttta agagttttaa taagttttaa agagttttag gcggaaaaat
     5761 cgcctttttt ctcttttata tcagtcactt acatgtgtga ccggttccca atgtacggct
     5821 ttgggttccc aatgtacggg ttccggttcc caatgtacgg ctttgggttc ccaatgtacg
     5881 tgctatccac aggaaagaag ctttttcgac ctttttcccc tgctagggca atttgcccta
     5941 gcatctgctc cgtacattag gaaccggcgg atgcttcgcc ctcgatcagg ttgcggtagc
     6001 gcatgactag gatcgggcca gcctgccccg cctcctcctt caaatcgtac tccggcaggt
     6061 catttgaccc gatcagcttg cgcacggtga aacagaactt cttgaactct ccggcgctgc
     6121 cactgcgttc gtagatcgtc ttgaacaacc atctggcttc tgccttgcct gcggcgcggc
     6181 gtgccaggcg gtagagaaaa cggccgatgc cgggatcgat caaaaagtaa tcggggtgaa
     6241 ccgtcagcac gtccgggttc ttgccttctg tgatctcgcg gtacatccaa tcagctagct
     6301 cgatctcgat gtactccggc cgcccggttt cgctctttac gatcttgtag cggctaatca
     6361 aggcttcacc ctcggatacc gtcaccaggc ggccgttctt ggccttcttc gtacgctgca
     6421 tggcaacgtg cgtggtgttt aaccgaatgc aggtttctac caggtcgtct ttctgctttc
     6481 cgccatcggc tcgccggcag aacttgagta cgtccgcaac gtgtggacgg aacacgcggc
     6541 cgggcttgtc tcccttccct tcccggtatc ggttcatgga ttcggttaga tgggaaaccg
     6601 ccatcagtac caggtcgtaa tcccacacac tggccatgcc ggccggccct gcggaaacct
     6661 ctacgtgccc gtctggaagc tcgtagcgga tcacctcgcc agctcgtcgg tcacgcttcg
     6721 acagacggaa aacggccacg tccatgatgc tgcgactatc gcgggtgccc acgtcataga
     6781 gcatcggaac gaaaaaatct ggttgctcgt cgcccttggg cggcttccta atcgacgacg
     6841 caccggctgc cggcggttgc cgggattctt tgcggattcg atcagcggcc gcttgccacg
     6901 attcaccggg gcgtgcttct gcctcgatgc gttgccgctg ggcggcctgc gcggccttca
     6961 acttctccac caggtcatca cccagcgccg cgccgatttg taccgggccg gatggtttgc
     7021 gaccgctcac gccgattcct cgggcttggg ggttccagtg ccattgcagg gccggcagac
     7081 aacccagccg cttacgcctg gccaaccgcc cgttcctcca cacatggggc attccacggc
     7141 gtcggtgcct ggttgttctt gattttccat gccgcctcct ttagccgcta aaattcatct
     7201 actcatttat tcatttgctc atttactctg gtagctgcgc gatgtattca gatagcagct
     7261 cggtaatggt cttgccttgg cgtaccgcgt acatcttcag cttggtgtga tcctccgccg
     7321 gcaactgaaa gttgacccgc ttcatggctg gcgtgtctgc caggctggcc aacgttgcag
     7381 ccttgctgct gcgtgcgctc ggacggccgg cacttagcgt gtttgtgctt ttgctcattt
     7441 tctctttacc tcattaactc aaatgagttt tgatttaatt tcagcggcca gcgcctggac
     7501 ctcgcgggca gcgtcgccct cgggttctga ttcaagaacg gttgtgccgg cggcggcagt
     7561 gcctgggtag ctcacgcgct gcgtgatacg ggactcaaga atgggcagct cgtacccggc
     7621 cagcgcctcg gcaacctcac cgccgatgcg cgtgcctttg atcgcccgcg acacgacaaa
     7681 ggccgcttgt agccttccat ccgtgacctc aatgcgctgc ttaaccagct ccaccaggtc
     7741 ggcggtggcc catatgtcgt aagggcttgg ctgcaccgga atcagcacga agtcggctgc
     7801 cttgatcgcg gacacagcca agtccgccgc ctggggcgct ccgtcgatca ctacgaagtc
     7861 gcgccggccg atggccttca cgtcgcggtc aatcgtcggg cggtcgatgc cgacaacggt
     7921 tagcggttga tcttcccgca cggccgccca atcgcgggca ctgccctggg gatcggaatc
     7981 gactaacaga acatcggccc cggcgagttg cagggcgcgg gctagatggg ttgcgatggt
     8041 cgtcttgcct gacccgcctt tctggttaag tacagcgata accttcatgc gttccccttg
     8101 cgtatttgtt tatttactca tcgcatcata tacgcagcga ccgcatgacg caagctgttt
     8161 tactcaaata cacatcacct ttttagacgg cggcgctcgg tttcttcagc ggccaagctg
     8221 gccggccagg ccgccagctt ggcatcagac aaaccggcca ggatttcatg cagccgcacg
     8281 gctgatgggc tgcctgtatc gagtggtgat tttgtgccga gctgccggtc ggggagctgt
     8341 tggctggctg gtggcaggat atattgtggt gtaaacaaat tgacgcttag acaacttaat
     8401 aacacattgc ggacgttttt aatgtactgg ggtggatgca gtgggcccca ctctgtggaa
     8461 gacaatgccc cgcggtccgg agtaacagat aaagcgaagc ttgaaggtgc atgtgactcc
     8521 gtcaagatta cgaaaccgcc aactaccacg caaattgcaa ttctcaattt cctagaagga
     8581 ctctccgaaa atgcatccaa taccaaatat tacccgtgtc ataggcacca agtgacacca
     8641 tacatgaaca cgcgtcacaa tatgactgga gaagggttcc acaccttatg ctataaaacg
     8701 ccccacaccc ctcctccttc cttcgcagtt caattccaat atattccatt ctctctgtgt
     8761 atttccctac ctctcccttc aaggttagtc gatttcttct gtttttcttc ttcgttcttt
     8821 ccatgaattg tgtatgttct ttgatcaata cgatgttgat ttgattgtgt tttgtttggt
     8881 ttcatcgatc ttcaattttc ataatcagat tcagctttta ttatctttac aacaacgtcc
     8941 ttaatttgat gattctttaa tcgtagattt gctctaatta gagctttttc atgtcagatc
     9001 cctttacaac aagccttaat tgttgattca ttaatcgtag attagggctt ttttcattga
     9061 ttacttcaga tccgttaaac gtaaccatag atcagggctt tttcatgaat tacttcagat
     9121 ccgttaaaca acagccttat tttttatact tctgtggttt ttcaagaaat tgttcagatc
     9181 cgttgacaaa aagccttatt cgttgattct atatcgtttt tcgagagata ttgctcagat
     9241 ctgttagcaa ctgccttgtt tgttgattct attgccgtgg attagggttt tttttcacga
     9301 gattgcttca gatccgtact taagattacg taatggattt tgattctgat ttatctgtga
     9361 ttgttgactc gacagcggcc gcacccccgg gggcgcgccc ttaacaaaaa tggcaaggga
     9421 ccccccagtc gcgaccatga ttcctctgtt aaacgcaaag cagattctcg ggcagttgga
     9481 tggggggagt gtggcggtcc tgccagtagt cgatagtact aatcaatatc tgctagatag
     9541 aataggtgag ttaaaaagcg gagacgcgtg catagccgaa tatcaacaag cgggaagagg
     9601 ctcacgagga aggaagtggt ttagcccatt cggggcgaat ctgtatcttt ctatgttctg
     9661 gcgtctgaag agaggcccgg cagcgattgg attaggcccg gtcataggca tcgttatggc
     9721 tgaggcttta agaaagctag gtgctgacaa agtgcgagtg aagtggccaa atgacttata
     9781 cttgcaagac aggaagctag cagggatttt agtcgagcta gccggtatca caggtgacgc
     9841 tgcacaaatt gttatagggg ccggtatcaa tgtggccatg cgtagagtgg aagaaagcgt
     9901 cgtgaaccaa ggatggatca ctctacagga ggctggaatt aatttggatc gaaacacctt
     9961 agctgcgatg ctaattagag aactgcgtgc ggcactggag ctgttcgagc aggaaggctt
    10021 ggccccctac ctatcaaggt gggaaaagct ggataacttc attaaccgtc cagtgaaatt
    10081 gattattgga gataaggaaa tctttggcat ctcacgaggg attgacaagc aaggggcatt
    10141 actgcttgaa caggatgggg ttattaagcc ttggatggga ggggaaatct ccttaaggtc
    10201 agcggagaag gcgtccggga agccaatccc aaatcccctt ctcggtcttg acagtaccgg
    10261 aggtggttct ggtaccacaa gtttgtacaa aaaagctgaa cgagaaacgt aaaatgatat
    10321 aaatatcaat atattaaatt agattttgca taaaaaacag actacataat actgtaaaac
    10381 acaacatatc cagtcactat ggcggccgca ttaggcaccc caggctttac actttatgct
    10441 tccggctcgt ataatgtgtg gattttgagt taggatccgt cgagattttc aggagctaag
    10501 gaagctaaaa tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat
    10561 cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt
    10621 cagctggata ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg
    10681 gcctttattc acattcttgc ccgcctgatg aatgctcatc cggaattccg tatggcaatg
    10741 aaagacggtg agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag
    10801 ca
//
