LOCUS       sequence-464386-        9932 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GATEWAY compatible vector with a soybean Ubiquitin (GmUbi) promoter 
            driving in frame epitope fusion of ccdb selection toxin and mCherry 
            marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9932)
  TITLE     GATEWAY compatible vector with a promoter driving in frame epitope 
            fusion paired with ccdb selection toxin and FP marker
REFERENCE   2  (bases 1 to 9932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Ubi:ccdb + mcherry
FEATURES             Location/Qualifiers
     source          1..9932
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     485..504
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        complement(join(904..909,910..926,927..932))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     rep_origin      1225..1419
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(1485..2558)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(2987..3616)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    3880..3904
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     protein_bind    4938..5062
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(5087..5092,5093..5110,5111..5117)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             5171..5830
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(5237..5256)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             6172..6477
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     6356..6375
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(6518..6642)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             7445..8155
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     7858..7877
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     terminator      8164..8416
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    8471..8495
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(8618..9206)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8698..8717)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9598..9932,1..856))
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
ORIGIN
        1 ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga
       61 cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg
      121 tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc
      181 ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg
      241 cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct
      301 tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg
      361 ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg
      421 ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc
      481 catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg
      541 ccacgatgcg tccggcgtag aggatccaca ggacgggtgt ggtcgccatg atcgcgtagt
      601 cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca
      661 gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc
      721 catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg
      781 gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg
      841 cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac
      901 cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaaa atattgcgct
      961 gccatttttg gggtgaggcc gttcgcggcc gaggggcgca gcccctgggg ggatgggagc
     1021 tcggtgcctt gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg tccgcgccct
     1081 ggtagattgc ctggccgtag gccagccatt tttgagcggc cagcggccgc gataggccga
     1141 cgcgaagcgg cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt ttgcagctct
     1201 tcggctgtgc gctggccaga cagttatgca caggccaggc gggttttaag agttttaata
     1261 agttttaaag agttttaggc ggaaaaatcg ccttttttct cttttatatc agtcacttac
     1321 atgtgtgacc ggttcccaat gtacggcttt gggttcccaa tgtacgggtt ccggttccca
     1381 atgtacggct ttgggttccc aatgtacgtg ctatccacag gaaagaagct ttttcgacct
     1441 ttttcccctg ctagggcaat ttgccctagc atctgctccg tacattagga accggcggat
     1501 gcttcgccct cgatcaggtt gcggtagcgc atgactagga tcgggccagc ctgccccgcc
     1561 tcctccttca aatcgtactc cggcaggtca tttgacccga tcagcttgcg cacggtgaaa
     1621 cagaacttct tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt gaacaaccat
     1681 ctggcttctg ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg gccgatgccg
     1741 ggatcgatca aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt gccttctgtg
     1801 atctcgcggt acatccaatc agctagctcg atctcgatgt actccggccg cccggtttcg
     1861 ctctttacga tcttgtagcg gctaatcaag gcttcaccct cggataccgt caccaggcgg
     1921 ccgttcttgg ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa ccgaatgcag
     1981 gtttctacca ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa cttgagtacg
     2041 tccgcaacgt gtggacggaa cacgcggccg ggcttgtctc ccttcccttc ccggtatcgg
     2101 ttcatggatt cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc ccacacactg
     2161 gccatgccgg ccggccctgc ggaaacctct acgtgcccgt ctggaagctc gtagcggatc
     2221 acctcgccag ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc catgatgctg
     2281 cgactatcgc gggtgcccac gtcatagagc atcggaacga aaaaatctgg ttgctcgtcg
     2341 cccttgggcg gcttcctaat cgacgacgca ccggctgccg gcggttgccg ggattctttg
     2401 cggattcgat cagcggccgc ttgccacgat tcaccggggc gtgcttctgc ctcgatgcgt
     2461 tgccgctggg cggcctgcgc ggccttcaac ttctccacca ggtcatcacc cagcgccgcg
     2521 ccgatttgta ccgggccgga tggtttgcga ccgctcacgc cgattcctcg ggcttggggg
     2581 ttccagtgcc attgcagggc cggcagacaa cccagccgct tacgcctggc caaccgcccg
     2641 ttcctccaca catggggcat tccacggcgt cggtgcctgg ttgttcttga ttttccatgc
     2701 cgcctccttt agccgctaaa attcatctac tcatttattc atttgctcat ttactctggt
     2761 agctgcgcga tgtattcaga tagcagctcg gtaatggtct tgccttggcg taccgcgtac
     2821 atcttcagct tggtgtgatc ctccgccggc aactgaaagt tgacccgctt catggctggc
     2881 gtgtctgcca ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg acggccggca
     2941 cttagcgtgt ttgtgctttt gctcattttc tctttacctc attaactcaa atgagttttg
     3001 atttaatttc agcggccagc gcctggacct cgcgggcagc gtcgccctcg ggttctgatt
     3061 caagaacggt tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc gtgatacggg
     3121 actcaagaat gggcagctcg tacccggcca gcgcctcggc aacctcaccg ccgatgcgcg
     3181 tgcctttgat cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc gtgacctcaa
     3241 tgcgctgctt aaccagctcc accaggtcgg cggtggccca tatgtcgtaa gggcttggct
     3301 gcaccggaat cagcacgaag tcggctgcct tgatcgcgga cacagccaag tccgccgcct
     3361 ggggcgctcc gtcgatcact acgaagtcgc gccggccgat ggccttcacg tcgcggtcaa
     3421 tcgtcgggcg gtcgatgccg acaacggtta gcggttgatc ttcccgcacg gccgcccaat
     3481 cgcgggcact gccctgggga tcggaatcga ctaacagaac atcggccccg gcgagttgca
     3541 gggcgcgggc tagatgggtt gcgatggtcg tcttgcctga cccgcctttc tggttaagta
     3601 cagcgataac cttcatgcgt tccccttgcg tatttgttta tttactcatc gcatcatata
     3661 cgcagcgacc gcatgacgca agctgtttta ctcaaataca catcaccttt ttagacggcg
     3721 gcgctcggtt tcttcagcgg ccaagctggc cggccaggcc gccagcttgg catcagacaa
     3781 accggccagg atttcatgca gccgcacggc tgatgggctg cctgtatcga gtggtgattt
     3841 tgtgccgagc tgccggtcgg ggagctgttg gctggctggt ggcaggatat attgtggtgt
     3901 aaacaaattg acgcttagac aacttaataa cacattgcgg acgtttttaa tgtactgggg
     3961 tggatgcagt gggccccact ctgtggaaga caatgccccg cggtccggag taacagataa
     4021 agcgaagctt gaaggtgcat gtgactccgt caagattacg aaaccgccaa ctaccacgca
     4081 aattgcaatt ctcaatttcc tagaaggact ctccgaaaat gcatccaata ccaaatatta
     4141 cccgtgtcat aggcaccaag tgacaccata catgaacacg cgtcacaata tgactggaga
     4201 agggttccac accttatgct ataaaacgcc ccacacccct cctccttcct tcgcagttca
     4261 attccaatat attccattct ctctgtgtat ttccctacct ctcccttcaa ggttagtcga
     4321 tttcttctgt ttttcttctt cgttctttcc atgaattgtg tatgttcttt gatcaatacg
     4381 atgttgattt gattgtgttt tgtttggttt catcgatctt caattttcat aatcagattc
     4441 agcttttatt atctttacaa caacgtcctt aatttgatga ttctttaatc gtagatttgc
     4501 tctaattaga gctttttcat gtcagatccc tttacaacaa gccttaattg ttgattcatt
     4561 aatcgtagat tagggctttt ttcattgatt acttcagatc cgttaaacgt aaccatagat
     4621 cagggctttt tcatgaatta cttcagatcc gttaaacaac agccttattt tttatacttc
     4681 tgtggttttt caagaaattg ttcagatccg ttgacaaaaa gccttattcg ttgattctat
     4741 atcgtttttc gagagatatt gctcagatct gttagcaact gccttgtttg ttgattctat
     4801 tgccgtggat tagggttttt tttcacgaga ttgcttcaga tccgtactta agattacgta
     4861 atggattttg attctgattt atctgtgatt gttgactcga cagcggccgc acccccgggg
     4921 gcgcgccctt aggtaccaca agtttgtaca aaaaagctga acgagaaacg taaaatgata
     4981 taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa tactgtaaaa
     5041 cacaacatat ccagtcacta tggcggccgc attaggcacc ccaggcttta cactttatgc
     5101 ttccggctcg tataatgtgt ggattttgag ttaggatccg tcgagatttt caggagctaa
     5161 ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca
     5221 tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt
     5281 tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc
     5341 ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaattcc gtatggcaat
     5401 gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga
     5461 gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct
     5521 acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg
     5581 gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga
     5641 tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgg gcaaatatta
     5701 tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga
     5761 tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggg
     5821 cggggcgtaa acgcgtggat ccggcttact aaaagccaga taacagtatg cgtatttgcg
     5881 cgctgatttt tgcggtataa gaatatatac tgatatgtat acccgaagta tgtcaaaaag
     5941 aggtatgcta tgaagcagcg tattacagtg acagttgaca gcgacagcta tcagttgctc
     6001 aaggcatata tgatgtcaat atctccggtc tggtaagcac aaccatgcag aatgaagccc
     6061 gtcgtctgcg tgccgaacgc tggaaagcgg aaaatcagga agggatggct gaggtcgccc
     6121 ggtttattga aatgaacggc tcttttgctg acgagaacag gggctggtga aatgcagttt
     6181 aaggtttaca cctataaaag agagagccgt tatcgtctgt ttgtggatgt acagagtgat
     6241 attattgaca cgcccgggcg acggatggtg atccccctgg ccagtgcacg tctgctgtca
     6301 gataaagtct cccgtgaact ttacccggtg gtgcatatcg gggatgaaag ctggcgcatg
     6361 atgaccaccg atatggccag tgtgccggtc tccgttatcg gggaagaagt ggctgatctc
     6421 agccaccgcg aaaatgacat caaaaacgcc attaacctga tgttctgggg aatataaatg
     6481 tcaggctccc ttatacacag ccagtctgca ggtcgaccat agtgactgga tatgttgtgt
     6541 tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat tgatatttat
     6601 atcattttac gtttctcgtt cagctttctt gtacaaagtg gtcctaggtt cggttcgagt
     6661 attatggcat tgggaaaact gtttttcttg taccatttgt tgtgcttgta atttactgtg
     6721 ttttttattc ggttttcgct atcgaactgt gaaatggaaa tggatggaga agagttaatg
     6781 aatgatatgg tccttttgtt cattctcaaa ttaatattat ttgttttttc tcttatttgt
     6841 tgtgtgttga atttgaaatt ataagagata tgcaaacatt ttgttttgag taaaaatgtg
     6901 tcaaatcaag gttccagaag gtaattatcc aagatgtagc atcaagaatc caatgtttac
     6961 gggaaaaact atggaagtat tatgtgagct cagcaagaag cagatcaata tgcggcacat
     7021 atgcaaccta tgttcaaaaa tgaagaatgt acagatacaa gatcctatac tgccagaata
     7081 cgaagaagaa tacgtagaaa ttgaaaaaga agaaccaggc gaagaaaaga atcttgaaga
     7141 cgtaagcact gacgacaaca atgaaaagaa gaagataagg tcggtgattg tgaaagagac
     7201 atagaggaca catgtaaggt ggaaaatgta agggcggaaa gtaaccttat cacaaaggaa
     7261 tcttatcccc cactacttat ccttttatat ttttccgtgt catttttgcc cttgagtttt
     7321 cctatataag gaaccaagtt cggcatttgt gaaaacaaga aaaaatttgg tgtaagctat
     7381 tttctttgaa gtactgagga tacaacttca gagaaatttg taagtttgtg atccgcaaac
     7441 aaaaatggtg agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt
     7501 caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga
     7561 gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct
     7621 gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa
     7681 gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga
     7741 gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca
     7801 ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc
     7861 cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga
     7921 cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga
     7981 cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa
     8041 cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta
     8101 cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca agtgagatgt
     8161 gcagatcgtt caaacatttg gcaataaagt ttcttaagat tgaatcctgt tgccggtctt
     8221 gcgatgatta tcatataatt tctgttgaat tacgttaagc atgtaataat taacatgtaa
     8281 tgcatgacgt tatttatgag atgggttttt atgattagag tcccgcaatt atacatttaa
     8341 tacgcgatag aaaacaaaat atagcgcgca aactaggata aattatcgcg cgcggtgtca
     8401 tctatgttac tagatccgat gataagctgt caaacatgag ggacacgaag tggtttaaac
     8461 tatcagtgtt tgacaggata tattggcggg taaacctaag agaaaagagc gtttattaga
     8521 ataatcggat atttaaaagg gcgtgaaaag gtttatccgt tcgtccattt gtatgtgcca
     8581 accacaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     8641 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     8701 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     8761 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     8821 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     8881 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     8941 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     9001 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     9061 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     9121 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     9181 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     9241 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     9301 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     9361 aaacttggtc tgacagctaa aacaattcat ccagtaaaat ataatcgaat attatgcgca
     9421 cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg
     9481 acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt
     9541 gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca
     9601 ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag
     9661 ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc
     9721 cgtgacgatc agcggtccaa tgatcgaagt taggctggta agagccgcga gcgatccttg
     9781 aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat
     9841 cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc
     9901 gaacgccagc aagacgtagc ccagcgcgtc gg
//
