LOCUS       sequence-46443-e         923 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Zhang Y, Zhang F, Li X, Baller JA, Qi Y, Starker CG, Bogdanove AJ, 
            Voytas DF
  TITLE     TALENs enable efficient plant genome engineering.
  JOURNAL   Plant Physiol. 2012 Nov 2.
  PUBMED    23124327
REFERENCE   2  (bases 1 to 923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2012 Nov 2."
COMMENT     Sequence Label: pZHY500
FEATURES             Location/Qualifiers
     source          1..923
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    724..745
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(760..765,766..783,784..790)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    798..814
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     803..825
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     822..838
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     822..838
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     857..877
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        859..877
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 atgtaccagc tttaatcatt tctccaccaa tcaaaagctc ttctacacta agaacagctc
       61 cattacaatt agtgatatga ttcaatcttg taagctgagc tttgtagttt cctttgaaat
      121 gaccactcac aaacaagaac ttaaattctg ttactgaaga tggatagact ttccaccatt
      181 cattagggtt gatatgcttg tttcttgttt gattctcttc gacatatctt tgcatttcat
      241 ctgcttgacc aattggaaga ttataacctc ctgaatatgc cttagtatca acgatcacac
      301 cgtaatcaat aggagatccg acagtataaa ttgctccgtc tggtttcctt gatccaccca
      361 aatgtttacc acgataacca taaaccttca taaagaactc catcaccttc atttcaagga
      421 ttctatcctg agttgaattt cttgcgattt caatcaattc aatatattca tgtggcacat
      481 atttcaattt atgtctaagt tcagatttct tctcttccaa ttcagatttc actagctggg
      541 atccggcaac gcgatgggac gtgcgttcgc caatacggcg attgactctt ctgatcaatt
      601 ccggcgcgtg cggcaatccc tttttcactg catccatggc aggacgtccg ccgaggcagg
      661 ccaaggcgac gaggtggtcg ttggagacga ccggtggaaa gcgggcagtg agcgcaacgc
      721 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
      781 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
      841 tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg aggacctcga
      901 gggggggccc ggtacccaat tcg
//
