LOCUS       sequence-464453-       16186 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GATEWAY compatible vector with a dexamethasone (DEX)-inducible 
            promoter driving in frame epitope fusion miniTurbo-ID paired with 
            ccdb selection toxin and RUBY marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16186)
  TITLE     GATEWAY compatible vector with a promoter driving in frame epitope 
            fusion paired with ccdb selection toxin and FP marker
REFERENCE   2  (bases 1 to 16186)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: DEXsynth:ccdb-mTb+ RUBY
FEATURES             Location/Qualifiers
     source          1..16186
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     510..529
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        complement(join(929..934,935..951,952..957))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     rep_origin      1250..1444
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(1510..2583)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(3012..3641)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    3905..3929
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5181..5414
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     promoter        7025..7071
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     protein_bind    7115..7239
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(7264..7269,7270..7287,7288..7294)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             7348..8007
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(7414..7433)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             8349..8654
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     8533..8552
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(8695..8819)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             9643..9684
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             10478..14428
                     /label=RUBY
     CDS             11978..12034
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             12869..12925
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     terminator      14443..14695
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    14750..14774
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(14897..15485)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(14977..14996)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(15877..16186,1..881))
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
ORIGIN
        1 agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg
       61 aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat
      121 accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg
      181 acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt
      241 gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc
      301 aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt
      361 aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc
      421 aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc
      481 ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc
      541 gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat ccacaggacg
      601 ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact
      661 gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc
      721 aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata
      781 tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg
      841 acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt
      901 tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca
      961 tgagaattct tgaaaatatt gcgctgccat ttttggggtg aggccgttcg cggccgaggg
     1021 gcgcagcccc tggggggatg ggagctcggt gccttgatgt gggcgccggc ggtcgagtgg
     1081 cgacggcgcg gcttgtccgc gccctggtag attgcctggc cgtaggccag ccatttttga
     1141 gcggccagcg gccgcgatag gccgacgcga agcggcgggg cgtagggagc gcagcgaccg
     1201 aagggtaggc gctttttgca gctcttcggc tgtgcgctgg ccagacagtt atgcacaggc
     1261 caggcgggtt ttaagagttt taataagttt taaagagttt taggcggaaa aatcgccttt
     1321 tttctctttt atatcagtca cttacatgtg tgaccggttc ccaatgtacg gctttgggtt
     1381 cccaatgtac gggttccggt tcccaatgta cggctttggg ttcccaatgt acgtgctatc
     1441 cacaggaaag aagctttttc gacctttttc ccctgctagg gcaatttgcc ctagcatctg
     1501 ctccgtacat taggaaccgg cggatgcttc gccctcgatc aggttgcggt agcgcatgac
     1561 taggatcggg ccagcctgcc ccgcctcctc cttcaaatcg tactccggca ggtcatttga
     1621 cccgatcagc ttgcgcacgg tgaaacagaa cttcttgaac tctccggcgc tgccactgcg
     1681 ttcgtagatc gtcttgaaca accatctggc ttctgccttg cctgcggcgc ggcgtgccag
     1741 gcggtagaga aaacggccga tgccgggatc gatcaaaaag taatcggggt gaaccgtcag
     1801 cacgtccggg ttcttgcctt ctgtgatctc gcggtacatc caatcagcta gctcgatctc
     1861 gatgtactcc ggccgcccgg tttcgctctt tacgatcttg tagcggctaa tcaaggcttc
     1921 accctcggat accgtcacca ggcggccgtt cttggccttc ttcgtacgct gcatggcaac
     1981 gtgcgtggtg tttaaccgaa tgcaggtttc taccaggtcg tctttctgct ttccgccatc
     2041 ggctcgccgg cagaacttga gtacgtccgc aacgtgtgga cggaacacgc ggccgggctt
     2101 gtctcccttc ccttcccggt atcggttcat ggattcggtt agatgggaaa ccgccatcag
     2161 taccaggtcg taatcccaca cactggccat gccggccggc cctgcggaaa cctctacgtg
     2221 cccgtctgga agctcgtagc ggatcacctc gccagctcgt cggtcacgct tcgacagacg
     2281 gaaaacggcc acgtccatga tgctgcgact atcgcgggtg cccacgtcat agagcatcgg
     2341 aacgaaaaaa tctggttgct cgtcgccctt gggcggcttc ctaatcgacg acgcaccggc
     2401 tgccggcggt tgccgggatt ctttgcggat tcgatcagcg gccgcttgcc acgattcacc
     2461 ggggcgtgct tctgcctcga tgcgttgccg ctgggcggcc tgcgcggcct tcaacttctc
     2521 caccaggtca tcacccagcg ccgcgccgat ttgtaccggg ccggatggtt tgcgaccgct
     2581 cacgccgatt cctcgggctt gggggttcca gtgccattgc agggccggca gacaacccag
     2641 ccgcttacgc ctggccaacc gcccgttcct ccacacatgg ggcattccac ggcgtcggtg
     2701 cctggttgtt cttgattttc catgccgcct cctttagccg ctaaaattca tctactcatt
     2761 tattcatttg ctcatttact ctggtagctg cgcgatgtat tcagatagca gctcggtaat
     2821 ggtcttgcct tggcgtaccg cgtacatctt cagcttggtg tgatcctccg ccggcaactg
     2881 aaagttgacc cgcttcatgg ctggcgtgtc tgccaggctg gccaacgttg cagccttgct
     2941 gctgcgtgcg ctcggacggc cggcacttag cgtgtttgtg cttttgctca ttttctcttt
     3001 acctcattaa ctcaaatgag ttttgattta atttcagcgg ccagcgcctg gacctcgcgg
     3061 gcagcgtcgc cctcgggttc tgattcaaga acggttgtgc cggcggcggc agtgcctggg
     3121 tagctcacgc gctgcgtgat acgggactca agaatgggca gctcgtaccc ggccagcgcc
     3181 tcggcaacct caccgccgat gcgcgtgcct ttgatcgccc gcgacacgac aaaggccgct
     3241 tgtagccttc catccgtgac ctcaatgcgc tgcttaacca gctccaccag gtcggcggtg
     3301 gcccatatgt cgtaagggct tggctgcacc ggaatcagca cgaagtcggc tgccttgatc
     3361 gcggacacag ccaagtccgc cgcctggggc gctccgtcga tcactacgaa gtcgcgccgg
     3421 ccgatggcct tcacgtcgcg gtcaatcgtc gggcggtcga tgccgacaac ggttagcggt
     3481 tgatcttccc gcacggccgc ccaatcgcgg gcactgccct ggggatcgga atcgactaac
     3541 agaacatcgg ccccggcgag ttgcagggcg cgggctagat gggttgcgat ggtcgtcttg
     3601 cctgacccgc ctttctggtt aagtacagcg ataaccttca tgcgttcccc ttgcgtattt
     3661 gtttatttac tcatcgcatc atatacgcag cgaccgcatg acgcaagctg ttttactcaa
     3721 atacacatca cctttttaga cggcggcgct cggtttcttc agcggccaag ctggccggcc
     3781 aggccgccag cttggcatca gacaaaccgg ccaggatttc atgcagccgc acggctgatg
     3841 ggctgcctgt atcgagtggt gattttgtgc cgagctgccg gtcggggagc tgttggctgg
     3901 ctggtggcag gatatattgt ggtgtaaaca aattgacgct tagacaactt aataacacat
     3961 tgcggacgtt tttaatgtac tggggtggat gcagtgggcc ccactctgtg gaagacaatg
     4021 cctccggagt aacagataaa gcgaagcttg aaggtgcatg tgactccgtc aagattacga
     4081 aaccgccaac taccacgcaa attgcaattc tcaatttcct agaaggactc tccgaaaatg
     4141 catccaatac caaatattac ccgtgtcata ggcaccaagt gacaccatac atgaacacgc
     4201 gtcacaatat gactggagaa gggttccaca ccttatgcta taaaacgccc cacacccctc
     4261 ctccttcctt cgcagttcaa ttccaatata ttccattctc tctgtgtatt tccctacctc
     4321 tcccttcaag gttagtcgat ttcttctgtt tttcttcttc gttctttcca tgaattgtgt
     4381 atgttctttg atcaatacga tgttgatttg attgtgtttt gtttggtttc atcgatcttc
     4441 aattttcata atcagattca gcttttatta tctttacaac aacgtcctta atttgatgat
     4501 tctttaatcg tagatttgct ctaattagag ctttttcatg tcagatccct ttacaacaag
     4561 ccttaattgt tgattcatta atcgtagatt agggcttttt tcattgatta cttcagatcc
     4621 gttaaacgta accatagatc agggcttttt catgaattac ttcagatccg ttaaacaaca
     4681 gccttatttt ttatacttct gtggtttttc aagaaattgt tcagatccgt tgacaaaaag
     4741 ccttattcgt tgattctata tcgtttttcg agagatattg ctcagatctg ttagcaactg
     4801 ccttgtttgt tgattctatt gccgtggatt agggtttttt ttcacgagat tgcttcagat
     4861 ccgtacttaa gattacgtaa tggattttga ttctgattta tctgtgattg ttgactcgac
     4921 agcggccgca ccggcgcgcc atggcagatc caatgaagct actgtcttct atcgaacaag
     4981 catgcgatat ttgccgactt aaaaagctca agtgctccaa agaaaaaccg aagtgcgcca
     5041 agtgtctgaa gaacaactgg gagtgtcgct actctcccaa aaccaaaaga tctccgctga
     5101 ctagggcaca tctgacagaa gtggaatcaa ggctagaaag actggaacag ctatttctac
     5161 tgatttttcc aaggtcgagc gcccccccga ccgatgtcag cctgggggac gagctccact
     5221 tagacggcga ggacgtggcg atggcgcatg ccgacgcgct agacgatttc gatctggaca
     5281 tgttggggga cggggattcc ccgggtccgg gatttacccc ccacgactcc gccccctacg
     5341 gcgctctgga tatggccgac ttcgagtttg agcagatgtt taccgatgcc cttggaattg
     5401 acgagtacgg tggggatcca attcagcaag ccactgcagg agtctcacaa gacacttcgg
     5461 aaaatcctaa caaaacaata gttcctgctg cattaccaca gctcacccct accttggtgt
     5521 cactgctgga ggtgattgaa cccgaggtgt tgtatgcagg atatgatagc tctgttccag
     5581 attcagcatg gagaattatg accacactca acatgttagg tgggcgtcaa gtgattgcag
     5641 cagtgaaatg ggcaaaggcg ataccaggct tcagaaactt acacctggat gaccaaatga
     5701 ccctgctaca gtactcatgg atgtttctca tggcatttgc cctgggttgg agatcataca
     5761 gacaatcaag tggaaacctg ctctgctttg ctcctgatct gattattaat gagcagagaa
     5821 tgtctctacc ctgcatgtat gaccaatgta aacacatgct gtttgtctcc tctgaattac
     5881 aaagattgca ggtatcctat gaagagtatc tctgtatgaa aaccttactg cttctctcct
     5941 cagttcctaa ggaaggtctg aagagccaag agttatttga tgagattcga atgacttata
     6001 tcaaagagct aggaaaagcc atcgtcaaaa gggaagggaa ctccagtcag aactggcaac
     6061 ggttttacca actgacaaag cttctggact ccatgcatga ggtggttgag aatctcctta
     6121 cctactgctt ccagacattt ttggataaga ccatgagtat tgaattccca gagatgttag
     6181 ctgaaatcat cactaatcag ataccaaaat attcaaatgg aaatatcaaa aagcttctgt
     6241 ttcatcaaaa atgactcgac ctaactgagt aagctagctt gttcgagtat tatggcattg
     6301 ggaaaactgt ttttcttgta ccatttgttg tgcttgtaat ttactgtgtt ttttattcgg
     6361 ttttcgctat cgaactgtga aatggaaatg gatggagaag agttaatgaa tgatatggtc
     6421 cttttgttca ttctcaaatt aatattattt gttttttctc ttatttgttg tgtgttgaat
     6481 ttgaaattat aagagatatg caaacatttt gttttgagta aaaatgtgtc aaatcgtggc
     6541 ctctaatgac cgaagttaat atgaggagta aaacactaga tccccaaaca agcttgaaac
     6601 tgaaggcggg aaacgacaat ctgatcatga gcggagaatt aagggagtca cgttatgacc
     6661 cccgccgatg acgcgggaca agccgtttta cgtttggaac tgacatatga aaatattttt
     6721 taatgcattt tatgacttgc caattgattg acaacatgca tcaatccgcg gttatgactc
     6781 tcttaagaga gtcatgaatt cgagcgggaa ttgatccccc ctcgacagct tgcatgccgg
     6841 tcgactctag aggatccggg tgacagccct ccgacgggtg acagccctcc gacgggtgac
     6901 agccctccga attctagagg atccgggtga cagccctccg acgggtgaca gccctccgac
     6961 gggtgacagc cctccgaatt cgagctcagt acccggggat ctgtcgacct cgatcgagat
     7021 cttcgcaaga cccttcctct atataaggaa gttcatttca tttggagagg acacgctgaa
     7081 gctagtcgac tctagcctcg aatccttagg taccacaagt ttgtacaaaa aagctgaacg
     7141 agaaacgtaa aatgatataa atatcaatat attaaattag attttgcata aaaaacagac
     7201 tacataatac tgtaaaacac aacatatcca gtcactatgg cggccgcatt aggcacccca
     7261 ggctttacac tttatgcttc cggctcgtat aatgtgtgga ttttgagtta ggatccgtcg
     7321 agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt
     7381 gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt
     7441 acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat
     7501 aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg
     7561 gaattccgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt
     7621 tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac
     7681 gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg
     7741 gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa tccctgggtg
     7801 agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc
     7861 accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt
     7921 catcatgccg tttgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac
     7981 tgcgatgagt ggcagggcgg ggcgtaaacg cgtggatccg gcttactaaa agccagataa
     8041 cagtatgcgt atttgcgcgc tgatttttgc ggtataagaa tatatactga tatgtatacc
     8101 cgaagtatgt caaaaagagg tatgctatga agcagcgtat tacagtgaca gttgacagcg
     8161 acagctatca gttgctcaag gcatatatga tgtcaatatc tccggtctgg taagcacaac
     8221 catgcagaat gaagcccgtc gtctgcgtgc cgaacgctgg aaagcggaaa atcaggaagg
     8281 gatggctgag gtcgcccggt ttattgaaat gaacggctct tttgctgacg agaacagggg
     8341 ctggtgaaat gcagtttaag gtttacacct ataaaagaga gagccgttat cgtctgtttg
     8401 tggatgtaca gagtgatatt attgacacgc ccgggcgacg gatggtgatc cccctggcca
     8461 gtgcacgtct gctgtcagat aaagtctccc gtgaacttta cccggtggtg catatcgggg
     8521 atgaaagctg gcgcatgatg accaccgata tggccagtgt gccggtctcc gttatcgggg
     8581 aagaagtggc tgatctcagc caccgcgaaa atgacatcaa aaacgccatt aacctgatgt
     8641 tctggggaat ataaatgtca ggctccctta tacacagcca gtctgcaggt cgaccatagt
     8701 gactggatat gttgtgtttt acagtattat gtagtctgtt ttttatgcaa aatctaattt
     8761 aatatattga tatttatatc attttacgtt tctcgttcag ctttcttgta caaagtggtc
     8821 ctaggcggag gtggttctgc aagggacccc ccagtcgcga ccatgattcc tctgttaaac
     8881 gcaaagcaga ttctcgggca gttggatggg gggagtgtgg cggtcctgcc agtagtcgat
     8941 agtactaatc aatatctgct agatagaata ggtgagttaa aaagcggaga cgcgtgcata
     9001 gccgaatatc aacaagcggg aagaggctca cgaggaagga agtggtttag cccattcggg
     9061 gcgaatctgt atctttctat gttctggcgt ctgaagagag gcccggcagc gattggatta
     9121 ggcccggtca taggcatcgt tatggctgag gctttaagaa agctaggtgc tgacaaagtg
     9181 cgagtgaagt ggccaaatga cttatacttg caagacagga agctagcagg gattttagtc
     9241 gagctagccg gtatcacagg tgacgctgca caaattgtta taggggccgg tatcaatgtg
     9301 gccatgcgta gagtggaaga aagcgtcgtg aaccaaggat ggatcactct acaggaggct
     9361 ggaattaatt tggatcgaaa caccttagct gcgatgctaa ttagagaact gcgtgcggca
     9421 ctggagctgt tcgagcagga aggcttggcc ccctacctat caaggtggga aaagctggat
     9481 aacttcatta accgtccagt gaaattgatt attggagata aggaaatctt tggcatctca
     9541 cgagggattg acaagcaagg ggcattactg cttgaacagg atggggttat taagccttgg
     9601 atgggagggg aaatctcctt aaggtcagcg gagaaggcgt ccgggaagcc aatcccaaat
     9661 ccccttctcg gtcttgacag tacctgattc ggttcgagta ttatggcatt gggaaaactg
     9721 tttttcttgt accatttgtt gtgcttgtaa tttactgtgt tttttattcg gttttcgcta
     9781 tcgaactgtg aaatggaaat ggatggagaa gagttaatga atgatatggt ccttttgttc
     9841 attctcaaat taatattatt tgttttttct cttatttgtt gtgtgttgaa tttgaaatta
     9901 taagagatat gcaaacattt tgttttgagt aaaaatgtgt caaatcaagg ttccagaagg
     9961 taattatcca agatgtagca tcaagaatcc aatgtttacg ggaaaaacta tggaagtatt
    10021 atgtgagctc agcaagaagc agatcaatat gcggcacata tgcaacctat gttcaaaaat
    10081 gaagaatgta cagatacaag atcctatact gccagaatac gaagaagaat acgtagaaat
    10141 tgaaaaagaa gaaccaggcg aagaaaagaa tcttgaagac gtaagcactg acgacaacaa
    10201 tgaaaagaag aagataaggt cggtgattgt gaaagagaca tagaggacac atgtaaggtg
    10261 gaaaatgtaa gggcggaaag taaccttatc acaaaggaat cttatccccc actacttatc
    10321 cttttatatt tttccgtgtc atttttgccc ttgagttttc ctatataagg aaccaagttc
    10381 ggcatttgtg aaaacaagaa aaaatttggt gtaagctatt ttctttgaag tactgaggat
    10441 acaacttcag agaaatttgt aagtttgtga tccgcaaatg gatcatgcga ccctcgccat
    10501 gatcctcgcg atctggttca tcagcttcca cttcatcaag ctgctgttct cccagcagac
    10561 caccaagctg cttccgccag gaccaaagcc gcttccgatc atcggcaaca tccttgaggt
    10621 gggcaagaag ccgcatcggt ccttcgccaa cctcgccaag attcacggcc cactcatttc
    10681 cctcagactc ggctctgtga ccaccatcgt tgtgtcctct gccgacgtgg ccaaagagat
    10741 gttcctcaag aaggatcacc cgctctccaa ccgcacgatc ccgaatagtg ttacagccgg
    10801 cgaccaccac aagctcacca tgtcttggct cccggtgtct ccgaagtggc gcaacttccg
    10861 caagattacc gccgtgcatc tgctctcccc acagagactc gatgcctgcc agacattcag
    10921 gcacgccaag gtgcagcagc tctacgagta cgttcaagag tgcgcccaga aaggccaggc
    10981 cgtggatatt ggcaaggccg cctttacgac cagcctcaac ctcctcagca agctgttctt
    11041 cagcgtcgag ctggcgcacc acaagtccca taccagccaa gagttcaaag agctgatctg
    11101 gaacatcatg gaagatatag gcaagccgaa ctacgccgac tacttcccga ttctcggctg
    11161 cgttgaccca tctggcatta gaagaaggct cgcctgctcc ttcgacaagc tgatcgccgt
    11221 gttccagggc atcatctgcg agagactcgc cccagattcc tccaccacaa ctaccaccac
    11281 caccgacgac gtgctcgatg tgctcctcca gctgttcaag cagaacgagc tgacgatggg
    11341 cgagatcaac cacctcctcg tggacatctt cgacgccggc accgatacca catcctccac
    11401 attcgagtgg gtgatgaccg agctgatccg caatccagag atgatggaaa aggcccaaga
    11461 ggaaatcaag caggtcctcg gcaaggacaa gcagatccaa gagtccgaca tcatcaacct
    11521 gccgtacctc caggcgatca tcaaagagac actccgcctc catccgccga ccgtgttctt
    11581 gctcccaaga aaggccgaca ccgatgtcga gctgtacggc tacatcgtgc cgaaggatgc
    11641 ccagatcctc gtgaacctct gggccattgg cagggaccca aacgcctggc agaacgccga
    11701 tattttcagc ccagagcgct tcatcggctg cgagatcgat gttaagggcc gcgatttcgg
    11761 cctccttcca tttggcgctg gccgcagaat ttgcccaggc atgaatctcg ccatcaggat
    11821 gctcaccctc atgctcgcca cactcctcca gttcttcaac tggaagctcg aaggcgacat
    11881 ctccccgaag gacctcgaca tggacgagaa gttcggcatt gcgctccaaa agaccaagcc
    11941 gctcaagctc atcccgattc cgcgctacgg tagcggagct accaatttta gcctccttaa
    12001 gcaggcaggt gatgtagaag agaaccccgg gcctatgaag atgatgaacg gcgaggacgc
    12061 caacgaccag atgatcaaag agtccttctt catcacccac ggcaacccga tcctcaccgt
    12121 cgaggataca catccgctca ggccgttctt cgagacatgg cgcgagaaga ttttctccaa
    12181 gaagccgaag gccatcctca tcatctccgg ccactgggag acagtgaagc caaccgtgaa
    12241 cgccgtgcac atcaacgaca ccatccacga cttcgacgac tacccagccg ccatgtacca
    12301 gttcaagtac ccagctccag gcgagccaga gcttgcgaga aaggtggaag agatcctcaa
    12361 gaagtccggg ttcgagacag ccgagacaga ccaaaagagg ggccttgatc acggcgcctg
    12421 ggttccactc atgctcatgt atccagaggc ggacatcccg gtgtgccagc tctcagttca
    12481 gccacatctc gacggcacct accactacaa tctcggcaga gccctcgcgc cgctcaagaa
    12541 tgatggcgtg ctcattattg gctccggcag cgccacacat ccactcgatg agacaccgca
    12601 ctacttcgat ggtgttgccc cttgggccgc tgccttcgat tcttggctta ggaaggccct
    12661 catcaacggc cgcttcgagg aagtgaacat ctacgagagc aaggccccga actggaagct
    12721 cgcccatcca tttccagagc acttctaccc gctccacgtt gtgctcggcg ctgctggtga
    12781 aaagtggaag gccgagctga tccactcctc ctgggatcat ggcacacttt gccacggctc
    12841 ctacaagttc acctccgccg gatccggagc aaccaacttt agcctgctca agcaagcagg
    12901 agatgttgag gaaaatcctg gccccatgac cgccatcaag atgaacacca acggcgaggg
    12961 cgagacacag cacatcctca tgatcccgtt catggcgcag ggccacctca ggccatttct
    13021 cgaactcgcc atgttcctct acaagcgctc ccacgtgatc atcaccctgc tcacaactcc
    13081 gctcaatgcc ggcttcctca ggcacctcct tcaccaccat tcctactcct ccagcggcat
    13141 caggatcgtc gagctgccat tcaactccac caaccacgga ctcccaccgg gcatcgagaa
    13201 caccgataag ctcacactcc cgctcgtggt gtccctcttc cattccacca tcagcctcga
    13261 tccgcacctc cgcgattaca tctccaggca tttcagccca gccaggccac cactctgcgt
    13321 gatccatgat gtgttcctcg gctgggttga ccaggtggcc aaggatgtgg gctctacagg
    13381 cgtggtgttc acaacaggcg gcgcttatgg cacatccgcc tacgtgtcca tctggaacga
    13441 tctcccgcac cagaactact ccgacgacca agagttcccg ctgccaggct tcccagagaa
    13501 ccataagttc cgcaggtccc agctccatcg gttcctcaga tatgccgacg gctccgacga
    13561 ttggtccaag tatttccagc cgcagctccg ccagtccatg aagtcttttg gctggctctg
    13621 caactccgtg gaagagatcg agacactcgg cttctccatc ctccgcaact acaccaagct
    13681 gccgatctgg ggcatcggcc cacttattgc ttccccagtg cagcactcct cctccgacaa
    13741 caattcaaca ggcgccgagt tcgtgcagtg gctcagcctc aaagagccgg actccgtcct
    13801 ctacatctcc ttcggctccc agaacacgat cagcccgacg cagatgatgg aactcgctgc
    13861 tggccttgag tcctccgaga agccattcct ctgggtgatc agagccccgt tcggcttcga
    13921 catcaacgaa gagatgcgcc cagagtggct gccagagggc tttgaggaac gcatgaaggt
    13981 gaagaaacag ggcaagctcg tgtacaagct cggcccgcag cttgagatcc tcaaccatga
    14041 atccatcggc ggctttctca cccactgcgg atggaacagc atccttgagt ctcttcgcga
    14101 gggcgttccg atgcttggat ggccacttgc tgccgagcag gcctacaacc tcaagtacct
    14161 cgaagatgag atgggcgtcg cggttgagct tgctagaggc ctcgaaggcg agatctccaa
    14221 agagaaggtc aagcgcatcg tcgagatgat ccttgagcgc aacgagggct ccaaaggctg
    14281 ggagatgaag aatcgcgccg tggaaatggg caaaaagctc aaggacgccg tgaacgagga
    14341 aaaagagctg aagggctcct ccgtgaaggc gatcgacgat ttcctcgacg ccgtcatgca
    14401 ggccaaactt gagccaagcc tccagtgata gtgagatgtg cagatcgttc aaacatttgg
    14461 caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt
    14521 ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga
    14581 tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata
    14641 tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact agatccgatg
    14701 ataagctgtc aaacatgagg gacacgaagt ggtttaaact atcagtgttt gacaggatat
    14761 attggcgggt aaacctaaga gaaaagagcg tttattagaa taatcggata tttaaaaggg
    14821 cgtgaaaagg tttatccgtt cgtccatttg tatgtgccaa ccacaggaac cgtaaaaagg
    14881 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
    14941 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
    15001 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
    15061 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
    15121 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
    15181 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
    15241 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
    15301 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
    15361 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
    15421 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
    15481 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
    15541 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
    15601 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagctaaa
    15661 acaattcatc cagtaaaata taatcgaata ttatgcgcac ccgtggccag gacccaacgc
    15721 tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc
    15781 aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag
    15841 tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca
    15901 tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc
    15961 caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccaat
    16021 gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc
    16081 atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag
    16141 aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgcc
//
