LOCUS       sequence-464454-       10794 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GATEWAY compatible vector with a soybean Ubiquitin (GmUbi) promoter 
            driving in frame epitope fusion of miniTurbo-ID paired with ccdb 
            selection toxin and mCherry marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10794)
  TITLE     GATEWAY compatible vector with a promoter driving in frame epitope 
            fusion paired with ccdb selection toxin and FP marker
REFERENCE   2  (bases 1 to 10794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Ubi:ccdb-mTb + mcherry
FEATURES             Location/Qualifiers
     source          1..10794
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     510..529
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        complement(join(929..934,935..951,952..957))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     rep_origin      1250..1444
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(1510..2583)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(3012..3641)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    3905..3929
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     protein_bind    4963..5087
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(5112..5117,5118..5135,5136..5142)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             5196..5855
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(5262..5281)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             6197..6502
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     6381..6400
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(6543..6667)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             7491..7532
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             8332..9042
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     8745..8764
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     terminator      9051..9303
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    9358..9382
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(9505..10093)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9585..9604)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10485..10794,1..881))
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
ORIGIN
        1 agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg
       61 aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat
      121 accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg
      181 acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt
      241 gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc
      301 aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt
      361 aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc
      421 aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc
      481 ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc
      541 gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat ccacaggacg
      601 ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact
      661 gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc
      721 aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata
      781 tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg
      841 acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt
      901 tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca
      961 tgagaattct tgaaaatatt gcgctgccat ttttggggtg aggccgttcg cggccgaggg
     1021 gcgcagcccc tggggggatg ggagctcggt gccttgatgt gggcgccggc ggtcgagtgg
     1081 cgacggcgcg gcttgtccgc gccctggtag attgcctggc cgtaggccag ccatttttga
     1141 gcggccagcg gccgcgatag gccgacgcga agcggcgggg cgtagggagc gcagcgaccg
     1201 aagggtaggc gctttttgca gctcttcggc tgtgcgctgg ccagacagtt atgcacaggc
     1261 caggcgggtt ttaagagttt taataagttt taaagagttt taggcggaaa aatcgccttt
     1321 tttctctttt atatcagtca cttacatgtg tgaccggttc ccaatgtacg gctttgggtt
     1381 cccaatgtac gggttccggt tcccaatgta cggctttggg ttcccaatgt acgtgctatc
     1441 cacaggaaag aagctttttc gacctttttc ccctgctagg gcaatttgcc ctagcatctg
     1501 ctccgtacat taggaaccgg cggatgcttc gccctcgatc aggttgcggt agcgcatgac
     1561 taggatcggg ccagcctgcc ccgcctcctc cttcaaatcg tactccggca ggtcatttga
     1621 cccgatcagc ttgcgcacgg tgaaacagaa cttcttgaac tctccggcgc tgccactgcg
     1681 ttcgtagatc gtcttgaaca accatctggc ttctgccttg cctgcggcgc ggcgtgccag
     1741 gcggtagaga aaacggccga tgccgggatc gatcaaaaag taatcggggt gaaccgtcag
     1801 cacgtccggg ttcttgcctt ctgtgatctc gcggtacatc caatcagcta gctcgatctc
     1861 gatgtactcc ggccgcccgg tttcgctctt tacgatcttg tagcggctaa tcaaggcttc
     1921 accctcggat accgtcacca ggcggccgtt cttggccttc ttcgtacgct gcatggcaac
     1981 gtgcgtggtg tttaaccgaa tgcaggtttc taccaggtcg tctttctgct ttccgccatc
     2041 ggctcgccgg cagaacttga gtacgtccgc aacgtgtgga cggaacacgc ggccgggctt
     2101 gtctcccttc ccttcccggt atcggttcat ggattcggtt agatgggaaa ccgccatcag
     2161 taccaggtcg taatcccaca cactggccat gccggccggc cctgcggaaa cctctacgtg
     2221 cccgtctgga agctcgtagc ggatcacctc gccagctcgt cggtcacgct tcgacagacg
     2281 gaaaacggcc acgtccatga tgctgcgact atcgcgggtg cccacgtcat agagcatcgg
     2341 aacgaaaaaa tctggttgct cgtcgccctt gggcggcttc ctaatcgacg acgcaccggc
     2401 tgccggcggt tgccgggatt ctttgcggat tcgatcagcg gccgcttgcc acgattcacc
     2461 ggggcgtgct tctgcctcga tgcgttgccg ctgggcggcc tgcgcggcct tcaacttctc
     2521 caccaggtca tcacccagcg ccgcgccgat ttgtaccggg ccggatggtt tgcgaccgct
     2581 cacgccgatt cctcgggctt gggggttcca gtgccattgc agggccggca gacaacccag
     2641 ccgcttacgc ctggccaacc gcccgttcct ccacacatgg ggcattccac ggcgtcggtg
     2701 cctggttgtt cttgattttc catgccgcct cctttagccg ctaaaattca tctactcatt
     2761 tattcatttg ctcatttact ctggtagctg cgcgatgtat tcagatagca gctcggtaat
     2821 ggtcttgcct tggcgtaccg cgtacatctt cagcttggtg tgatcctccg ccggcaactg
     2881 aaagttgacc cgcttcatgg ctggcgtgtc tgccaggctg gccaacgttg cagccttgct
     2941 gctgcgtgcg ctcggacggc cggcacttag cgtgtttgtg cttttgctca ttttctcttt
     3001 acctcattaa ctcaaatgag ttttgattta atttcagcgg ccagcgcctg gacctcgcgg
     3061 gcagcgtcgc cctcgggttc tgattcaaga acggttgtgc cggcggcggc agtgcctggg
     3121 tagctcacgc gctgcgtgat acgggactca agaatgggca gctcgtaccc ggccagcgcc
     3181 tcggcaacct caccgccgat gcgcgtgcct ttgatcgccc gcgacacgac aaaggccgct
     3241 tgtagccttc catccgtgac ctcaatgcgc tgcttaacca gctccaccag gtcggcggtg
     3301 gcccatatgt cgtaagggct tggctgcacc ggaatcagca cgaagtcggc tgccttgatc
     3361 gcggacacag ccaagtccgc cgcctggggc gctccgtcga tcactacgaa gtcgcgccgg
     3421 ccgatggcct tcacgtcgcg gtcaatcgtc gggcggtcga tgccgacaac ggttagcggt
     3481 tgatcttccc gcacggccgc ccaatcgcgg gcactgccct ggggatcgga atcgactaac
     3541 agaacatcgg ccccggcgag ttgcagggcg cgggctagat gggttgcgat ggtcgtcttg
     3601 cctgacccgc ctttctggtt aagtacagcg ataaccttca tgcgttcccc ttgcgtattt
     3661 gtttatttac tcatcgcatc atatacgcag cgaccgcatg acgcaagctg ttttactcaa
     3721 atacacatca cctttttaga cggcggcgct cggtttcttc agcggccaag ctggccggcc
     3781 aggccgccag cttggcatca gacaaaccgg ccaggatttc atgcagccgc acggctgatg
     3841 ggctgcctgt atcgagtggt gattttgtgc cgagctgccg gtcggggagc tgttggctgg
     3901 ctggtggcag gatatattgt ggtgtaaaca aattgacgct tagacaactt aataacacat
     3961 tgcggacgtt tttaatgtac tggggtggat gcagtgggcc ccactctgtg gaagacaatg
     4021 ccccgcggtc cggagtaaca gataaagcga agcttgaagg tgcatgtgac tccgtcaaga
     4081 ttacgaaacc gccaactacc acgcaaattg caattctcaa tttcctagaa ggactctccg
     4141 aaaatgcatc caataccaaa tattacccgt gtcataggca ccaagtgaca ccatacatga
     4201 acacgcgtca caatatgact ggagaagggt tccacacctt atgctataaa acgccccaca
     4261 cccctcctcc ttccttcgca gttcaattcc aatatattcc attctctctg tgtatttccc
     4321 tacctctccc ttcaaggtta gtcgatttct tctgtttttc ttcttcgttc tttccatgaa
     4381 ttgtgtatgt tctttgatca atacgatgtt gatttgattg tgttttgttt ggtttcatcg
     4441 atcttcaatt ttcataatca gattcagctt ttattatctt tacaacaacg tccttaattt
     4501 gatgattctt taatcgtaga tttgctctaa ttagagcttt ttcatgtcag atccctttac
     4561 aacaagcctt aattgttgat tcattaatcg tagattaggg cttttttcat tgattacttc
     4621 agatccgtta aacgtaacca tagatcaggg ctttttcatg aattacttca gatccgttaa
     4681 acaacagcct tattttttat acttctgtgg tttttcaaga aattgttcag atccgttgac
     4741 aaaaagcctt attcgttgat tctatatcgt ttttcgagag atattgctca gatctgttag
     4801 caactgcctt gtttgttgat tctattgccg tggattaggg ttttttttca cgagattgct
     4861 tcagatccgt acttaagatt acgtaatgga ttttgattct gatttatctg tgattgttga
     4921 ctcgacagcg gccgcacccc cgggggcgcg cccttaggta ccacaagttt gtacaaaaaa
     4981 gctgaacgag aaacgtaaaa tgatataaat atcaatatat taaattagat tttgcataaa
     5041 aaacagacta cataatactg taaaacacaa catatccagt cactatggcg gccgcattag
     5101 gcaccccagg ctttacactt tatgcttccg gctcgtataa tgtgtggatt ttgagttagg
     5161 atccgtcgag attttcagga gctaaggaag ctaaaatgga gaaaaaaatc actggatata
     5221 ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt cagtcagttg
     5281 ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta aagaccgtaa
     5341 agaaaaataa gcacaagttt tatccggcct ttattcacat tcttgcccgc ctgatgaatg
     5401 ctcatccgga attccgtatg gcaatgaaag acggtgagct ggtgatatgg gatagtgttc
     5461 acccttgtta caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat
     5521 accacgacga tttccggcag tttctacaca tatattcgca agatgtggcg tgttacggtg
     5581 aaaacctggc ctatttccct aaagggttta ttgagaatat gtttttcgtc tcagccaatc
     5641 cctgggtgag tttcaccagt tttgatttaa acgtggccaa tatggacaac ttcttcgccc
     5701 ccgttttcac catgggcaaa tattatacgc aaggcgacaa ggtgctgatg ccgctggcga
     5761 ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt aatgaattac
     5821 aacagtactg cgatgagtgg cagggcgggg cgtaaacgcg tggatccggc ttactaaaag
     5881 ccagataaca gtatgcgtat ttgcgcgctg atttttgcgg tataagaata tatactgata
     5941 tgtatacccg aagtatgtca aaaagaggta tgctatgaag cagcgtatta cagtgacagt
     6001 tgacagcgac agctatcagt tgctcaaggc atatatgatg tcaatatctc cggtctggta
     6061 agcacaacca tgcagaatga agcccgtcgt ctgcgtgccg aacgctggaa agcggaaaat
     6121 caggaaggga tggctgaggt cgcccggttt attgaaatga acggctcttt tgctgacgag
     6181 aacaggggct ggtgaaatgc agtttaaggt ttacacctat aaaagagaga gccgttatcg
     6241 tctgtttgtg gatgtacaga gtgatattat tgacacgccc gggcgacgga tggtgatccc
     6301 cctggccagt gcacgtctgc tgtcagataa agtctcccgt gaactttacc cggtggtgca
     6361 tatcggggat gaaagctggc gcatgatgac caccgatatg gccagtgtgc cggtctccgt
     6421 tatcggggaa gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa acgccattaa
     6481 cctgatgttc tggggaatat aaatgtcagg ctcccttata cacagccagt ctgcaggtcg
     6541 accatagtga ctggatatgt tgtgttttac agtattatgt agtctgtttt ttatgcaaaa
     6601 tctaatttaa tatattgata tttatatcat tttacgtttc tcgttcagct ttcttgtaca
     6661 aagtggtcct aggcggaggt ggttctgcaa gggacccccc agtcgcgacc atgattcctc
     6721 tgttaaacgc aaagcagatt ctcgggcagt tggatggggg gagtgtggcg gtcctgccag
     6781 tagtcgatag tactaatcaa tatctgctag atagaatagg tgagttaaaa agcggagacg
     6841 cgtgcatagc cgaatatcaa caagcgggaa gaggctcacg aggaaggaag tggtttagcc
     6901 cattcggggc gaatctgtat ctttctatgt tctggcgtct gaagagaggc ccggcagcga
     6961 ttggattagg cccggtcata ggcatcgtta tggctgaggc tttaagaaag ctaggtgctg
     7021 acaaagtgcg agtgaagtgg ccaaatgact tatacttgca agacaggaag ctagcaggga
     7081 ttttagtcga gctagccggt atcacaggtg acgctgcaca aattgttata ggggccggta
     7141 tcaatgtggc catgcgtaga gtggaagaaa gcgtcgtgaa ccaaggatgg atcactctac
     7201 aggaggctgg aattaatttg gatcgaaaca ccttagctgc gatgctaatt agagaactgc
     7261 gtgcggcact ggagctgttc gagcaggaag gcttggcccc ctacctatca aggtgggaaa
     7321 agctggataa cttcattaac cgtccagtga aattgattat tggagataag gaaatctttg
     7381 gcatctcacg agggattgac aagcaagggg cattactgct tgaacaggat ggggttatta
     7441 agccttggat gggaggggaa atctccttaa ggtcagcgga gaaggcgtcc gggaagccaa
     7501 tcccaaatcc ccttctcggt cttgacagta cctgattcgg ttcgagtatt atggcattgg
     7561 gaaaactgtt tttcttgtac catttgttgt gcttgtaatt tactgtgttt tttattcggt
     7621 tttcgctatc gaactgtgaa atggaaatgg atggagaaga gttaatgaat gatatggtcc
     7681 ttttgttcat tctcaaatta atattatttg ttttttctct tatttgttgt gtgttgaatt
     7741 tgaaattata agagatatgc aaacattttg ttttgagtaa aaatgtgtca aatcaaggtt
     7801 ccagaaggta attatccaag atgtagcatc aagaatccaa tgtttacggg aaaaactatg
     7861 gaagtattat gtgagctcag caagaagcag atcaatatgc ggcacatatg caacctatgt
     7921 tcaaaaatga agaatgtaca gatacaagat cctatactgc cagaatacga agaagaatac
     7981 gtagaaattg aaaaagaaga accaggcgaa gaaaagaatc ttgaagacgt aagcactgac
     8041 gacaacaatg aaaagaagaa gataaggtcg gtgattgtga aagagacata gaggacacat
     8101 gtaaggtgga aaatgtaagg gcggaaagta accttatcac aaaggaatct tatcccccac
     8161 tacttatcct tttatatttt tccgtgtcat ttttgccctt gagttttcct atataaggaa
     8221 ccaagttcgg catttgtgaa aacaagaaaa aatttggtgt aagctatttt ctttgaagta
     8281 ctgaggatac aacttcagag aaatttgtaa gtttgtgatc cgcaaacaaa aatggtgagc
     8341 aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg
     8401 gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac
     8461 gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg
     8521 gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac
     8581 atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac
     8641 ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc
     8701 atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag
     8761 aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag
     8821 ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag
     8881 accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag
     8941 ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag
     9001 ggccgccact ccaccggcgg catggacgag ctgtacaagt gagatgtgca gatcgttcaa
     9061 acatttggca ataaagtttc ttaagattga atcctgttgc cggtcttgcg atgattatca
     9121 tataatttct gttgaattac gttaagcatg taataattaa catgtaatgc atgacgttat
     9181 ttatgagatg ggtttttatg attagagtcc cgcaattata catttaatac gcgatagaaa
     9241 acaaaatata gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct atgttactag
     9301 atccgatgat aagctgtcaa acatgaggga cacgaagtgg tttaaactat cagtgtttga
     9361 caggatatat tggcgggtaa acctaagaga aaagagcgtt tattagaata atcggatatt
     9421 taaaagggcg tgaaaaggtt tatccgttcg tccatttgta tgtgccaacc acaggaaccg
     9481 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     9541 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     9601 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     9661 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     9721 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     9781 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     9841 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     9901 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     9961 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
    10021 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
    10081 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
    10141 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
    10201 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
    10261 cagctaaaac aattcatcca gtaaaatata atcgaatatt atgcgcaccc gtggccagga
    10321 cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat
    10381 gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat
    10441 tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc
    10501 cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca
    10561 atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc
    10621 ggtccaatga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga
    10681 tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg
    10741 gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgcc
//
