LOCUS       sequence-46446-e         665 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Xie J, Ameres SL, Friedline R, Hung JH, Zhang Y, Xie Q, Zhong L, Su 
            Q, He R, Li M, Li H, Mu X, Zhang H, Broderick JA, Kim JK, Weng Z, 
            Flotte TR, Zamore PD, Gao G
  TITLE     Long-term, efficient inhibition of microRNA function in mice using 
            rAAV vectors.
  JOURNAL   Nat Methods. 2012 Mar 4;9(4):403-9. doi: 10.1038/nmeth.1903.
  PUBMED    22388288
REFERENCE   2  (bases 1 to 665)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-03-4- 4"; volume: "9"; 
            issue: "4"; pages: "403-9"
COMMENT     Sequence Label: pAAVf-EnhCB-lacZnls mir-122 1xBS
FEATURES             Location/Qualifiers
     source          1..665
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             139..159
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 aggaactctg aggaagccag gacagacagg cccagccagc agctcaggtc tctcaatgga
       61 gagtggaggt ttgcctggtt ccctgcccct gaagctgtgc ctgagtcttg gctggagtgt
      121 gacctcccag aggcagttcc caagaagaag aggaaagttg aggctgacac tgttgtggtg
      181 ccaagcaact ggcagatgca tggctatgat gcccccatct acaccaatgt cacctacccc
      241 atcactgtga accccccttt tgtgcccact gagaacccca ctggctgcta cagcctgacc
      301 ttcaatgttg atgagagctg gctgcaagaa ggccagacca ggatcatctt tgatggagtc
      361 aactctgcct tccacctctg gtgcaatggc aggtgggttg gctatggcca agacagcagg
      421 ctgccctctg agtttgacct ctctgccttc ctcagagctg gagagaacag gctggctgtc
      481 atggtgctca ggtggtctga tggcagctac ctggaagacc aagacatgtg gaggatgtct
      541 ggcatcttca gggatgtgag cctgctgcac aagcccacca cccagatttc tgacttccat
      601 gttgccacca ggttcaatga tgacttcagc agagctgtgc tggaggctga ggtgcagatg
      661 tgtgg
//
