LOCUS       sequence-464530-        9464 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For expression of MERS-CoV spike protein with glycan deletion at 
            N1176, N1213, N1225 by implementing Asn-to-Gln mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9464)
  AUTHORS   Yang Q, Kelkar A, Manicassamy B, Neelamegham S
  TITLE     Conserved role of spike S2 domain N-glycosylation across 
            betacoronaviruses.
  JOURNAL   Npj Viruses. 2025 Jan 25;3(1):4. doi: 10.1038/s44298-024-00085-7.
  PUBMED    40295734
REFERENCE   2  (bases 1 to 9464)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Npj 
            Viruses."; date: "2025-01-25"; volume: "3(1):4. doi"; pages: " 
            10.1038/s44298-024-00085-7"
COMMENT     Sequence Label: MERS-CoV spike first3KO mutant
FEATURES             Location/Qualifiers
     source          1..9464
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        110..489
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        490..693
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     644..664
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     690..714
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     complement(4910..4929)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4954..5002
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5204..5632
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5291..5310)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5501..5522
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5641..5661)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5646..5975
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5826..5961
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5888..5907
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6042..6836
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6096..6115)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6706..6725
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7012..7133
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7049..7068)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7103..7122
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7182..7198)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7182..7198)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7195..7217)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7206..7222
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7230..7236,7237..7254,7255..7260))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7275..7296
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7413..7430)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7584..8172)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7664..7683)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8343..9134,9135..9203))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8966..8985
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9204..9308)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9383..9402)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg
       61 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
      121 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      181 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      241 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      301 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      361 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      421 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      481 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      541 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      601 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      661 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg
      721 agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgg
      781 actgagctcc ttaaggttaa ctctagagaa ttcggatccg ccaccatgat ccactccgtg
      841 tttctgctga tgttcctgct gacccctacc gagagctatg tggacgtggg ccccgattct
      901 gtgaagtccg cctgcatcga agtggacatc cagcagacct ttttcgacaa gacctggcct
      961 cggcctatcg acgtgtccaa ggccgacggc atcatctacc ctcaaggccg gacctacagc
     1021 aacatcacca ttacctacca ggggctgttc ccctaccaag gcgatcacgg cgatatgtac
     1081 gtgtactctg ccggacacgc caccggcacc acacctcaga aactgttcgt ggccaactac
     1141 agccaggacg tgaagcagtt cgccaacggc tttgttgtgc ggattggcgc cgctgccaat
     1201 agcacaggca cagtgatcat cagccctagc accagcgcca ccatccggaa gatctaccct
     1261 gcctttatgc tgggcagcag cgtgggcaat ttcagcgacg gcaagatggg cagattcttc
     1321 aatcacaccc tggtgctgct gcctgatggc tgtggaacac tgctgagagc cttctactgc
     1381 atcctggaac ctcggagcgg caatcactgt cctgccggca atagctacac cagcttcgcc
     1441 acctatcaca cccctgccac cgattgctcc gacggcaact acaacagaaa cgccagcctg
     1501 aacagcttca aagagtactt caacctgcgg aactgcacct tcatgtacac ctacaatatc
     1561 accgaggacg agatcctcga gtggttcggc atcacacaga cagctcaggg cgtgcacctg
     1621 ttcagcagca gatacgtgga cctgtacggc ggcaacatgt tccagtttgc cacactgccc
     1681 gtgtacgaca ccatcaagta ctacagcatc atccctcaca gcatccggtc catccagagc
     1741 gacagaaaag cctgggccgc cttctacgtg tacaagctcc agcctctgac cttcctgctg
     1801 gactttagcg tggacggcta catcagacgg gccatcgact gcggcttcaa cgatctgtct
     1861 cagctgcact gctcctacga gagcttcgac gtggaaagcg gcgtgtacag cgtgtccagc
     1921 tttgaggcca agcctagcgg ctctgtggtg gaacaagctg aaggcgtgga atgcgacttc
     1981 agccctctgc tgtctggcac acctcctcag gtgtacaact tcaagcggct ggtgttcacc
     2041 aactgcaatt acaacctgac caagctgctg agcctgttct ccgtgaacga cttcacctgt
     2101 agccagatca gccctgccgc cattgccagc aactgttaca gcagcctgat cctggactac
     2161 ttcagctacc ctctgagcat gaagtccgat ctgagcgtgt cctccgccgg accaatcagc
     2221 cagttcaact acaagcagtc cttcagcaac cctacctgcc tgattctggc caccgtgcct
     2281 cacaatctga ccaccatcac caagcctctg aagtacagct acatcaacaa gtgcagcaga
     2341 ctgctgagcg acgacagaac cgaagtgccc cagctggtca acgccaacca gtacagccct
     2401 tgcgtgtcca tcgtgcctag caccgtttgg gaggacggcg actactatcg gaagcagctg
     2461 tctcctctgg aaggcggagg atggctggtg gcctctggat ctacagtggc catgacagag
     2521 cagctccaga tgggctttgg catcaccgtg cagtacggca ccgacaccaa tagcgtgtgc
     2581 cctaagctgg aattcgccaa tgacaccaag atcgccagcc agctgggcaa ctgcgtggaa
     2641 tactctctct acggcgttag cggcagaggc gtgttccaga attgcaccgc cgtgggcgtc
     2701 cgccagcaga gatttgtgta cgacgcctac cagaacctcg tgggctacta cagcgacgac
     2761 ggcaattact actgcctgcg ggcctgtgtg tccgtgcctg tgtctgtgat ctacgacaaa
     2821 gagacaaaga cccacgccac actgttcggc agcgtggcct gtgaacacat cagctccacc
     2881 atgagccagt actccaggtc caccagatcc atgctgaagc ggcgggatag cacctacgga
     2941 cctctgcaaa cacctgtggg ctgtgtgctg ggcctcgtga atagcagcct gttcgtcgag
     3001 gactgcaagc tgcctctggg ccagtctttg tgtgccctgc cagacacacc ctccacactg
     3061 acacctagat ctgtgcgctc tgtgcccggc gaaatgagac tggcctctat cgcctttaat
     3121 caccccatcc aggtggacca gctgaactcc agctacttca agctgagcat ccccaccaac
     3181 ttcagcttcg gcgtgaccca agagtacatc cagaccacaa tccagaaagt gaccgtggat
     3241 tgcaagcagt acgtgtgcaa cggcttccag aagtgcgaac agctgctcag agagtacggc
     3301 cagttctgct ccaagatcaa tcaggccctg cacggcgcca acctgagaca ggatgatagc
     3361 gtgcggaacc tgttcgcctc cgtgaagtcc tctcagagca gccctatcat ccctggcttc
     3421 ggcggcgact ttaatctgac cctgctggaa cctgtgtcca tcagcaccgg ctccagaagc
     3481 gccagatctg ccattgagga cctgctgttc gacaaagtga ccattgccga tcctggctac
     3541 atgcagggct acgacgattg catgcagcag ggacctgcca gcgccaggga tctgatttgt
     3601 gcccagtatg tggccggcta caaggtgctg cctcctctga tggacgtgaa catggaagcc
     3661 gcctacacct ctagcctgct gggatctatt gctggcgttg gctggacagc cggcctgtct
     3721 agctttgccg ctattccctt cgctcagagc atcttctacc ggctgaatgg cgtcgggatc
     3781 acccagcagg ttctgagcga gaaccagaag ctgatcgcca acaagttcaa ccaggctctg
     3841 ggcgccatgc agaccggctt taccacaaca aacgaggcct ttcagaaagt gcaggacgcc
     3901 gtgaacaaca acgcccaggc actgtctaag ctggcctccg agctgagcaa taccttcgga
     3961 gccatctctg ccagcatcgg cgacatcatc cagagactgg acgtgctgga acaggacgcc
     4021 cagatcgaca gactgatcaa cggcagactg accacactga acgccttcgt ggcccagcag
     4081 ctcgtcagaa gtgaaagcgc tgcactgtct gctcagctgg ccaaggacaa agtcaacgag
     4141 tgcgtgaagg cccagagcaa gagatccggc ttttgtggcc agggcaccca catcgtgtcc
     4201 ttcgtggtca atgcccctaa cggcctgtac tttatgcacg tgggctatta ccccagcaac
     4261 cacatcgagg tggtgtccgc ctacggactg tgtgacgccg ccaatcctac caactgtatc
     4321 gcccctgtga acggctactt catcaagacc cagaacaccc ggatcgtgga cgagtggtct
     4381 tacaccggca gcagctttta cgcccctgag cctatcacct ctctgaacac caaatacgtg
     4441 gcccctcaag tgacatacca gcagatctcc accaacctgc ctccacctct gctgggccag
     4501 tccaccggca tcgatttcca ggacgagctg gacgagttct tcaagaacgt gtccaccagc
     4561 attcccaact tcggcagcct gacacagatc aacaccacac tgctggacct gacctacgaa
     4621 atgctgtccc tgcaacaggt ggtcaaggcc ctgaacgaga gctacatcga cctgaaagag
     4681 ctggggaact acacctacta caacaagtgg ccctggtaca tctggctggg ctttatcgct
     4741 ggactggtgg cactggccct gtgcgtgttc ttcatcctgt gctgtaccgg ctgcggcacc
     4801 aattgcatgg gcaagctgaa atgcaaccgg tgctgcgaca gataactcga gccatggaag
     4861 cttgatatct aactgactga accggtggta ccaaacgggg gaggctaact gaaacacgga
     4921 aggagacaat accggaagga acccgcgcta tgacggcaat aaaaagacag aataaaacgc
     4981 acgggtgttg ggtcgtttgt tcataaacgc ggggttcggt cccagggctg gcactctgtc
     5041 gataccccac cgagacccca ttggggccaa tacgcccgcg tttcttcctt ttccccaccc
     5101 caccccccaa gttcgggtga aggcccaggg ctcgcagcca acgtcggggc ggcaggccct
     5161 gccatagcag atctgcgcag ctggggctct agggggtatc cccacgcgcc ctgtagcggc
     5221 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     5281 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     5341 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     5401 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     5461 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     5521 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     5581 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt
     5641 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     5701 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
     5761 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
     5821 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     5881 ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt
     5941 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca
     6001 ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat
     6061 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
     6121 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
     6181 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc
     6241 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
     6301 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
     6361 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
     6421 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
     6481 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
     6541 cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga
     6601 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
     6661 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
     6721 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
     6781 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg
     6841 gactctgggg ttcgcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc
     6901 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
     6961 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt
     7021 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     7081 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     7141 tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg
     7201 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     7261 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     7321 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     7381 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     7441 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     7501 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     7561 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     7621 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     7681 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     7741 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     7801 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     7861 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     7921 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     7981 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     8041 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     8101 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     8161 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     8221 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     8281 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     8341 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     8401 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     8461 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     8521 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     8581 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     8641 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     8701 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     8761 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     8821 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     8881 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     8941 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     9001 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     9061 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     9121 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     9181 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     9241 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     9301 gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg
     9361 atcccctatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtatc
     9421 tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagc
//
