LOCUS       sequence-464545-         444 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid expresses human alpha-synuclein with a 7-amino acid 
            insertion (MAAAEKT) in the pRK172 backbone for bacterial expression 
            in E. coli. The insertion corresponds to the JOS mutation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Yang Y, Garringer HJ, Shi Y, Lovestam S, Peak-Chew S, Zhang X, 
            Kotecha A, Bacioglu M, Koto A, Takao M, Spillantini MG, Ghetti B, 
            Vidal R, Murzin AG, Scheres SHW, Goedert M
  TITLE     New SNCA mutation and structures of alpha-synuclein filaments from 
            juvenile-onset synucleinopathy.
  JOURNAL   Acta Neuropathol. 2023 May;145(5):561-572. doi: 
            10.1007/s00401-023-02550-8. Epub 2023 Feb 27.
  PUBMED    36847833
REFERENCE   2  (bases 1 to 444)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s00401-023-02550-8"; journalName: "Acta Neuropathol"; date:
            "2023-05"; volume: "145"; issue: "5"; pages: "561-572"
COMMENT     Sequence Label: 7AA-aSyn-pRK172
FEATURES             Location/Qualifiers
     source          1..444
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggatgtat tcatgaaagg actttcaaag gccaaggagg gagttgtggc tgctgctgag
       61 aaaaccatgg cagcagcaga aaagactaaa cagggtgtgg cagaagcagc aggaaagaca
      121 aaagagggtg ttctctatgt aggctccaaa accaaggagg gagtggtgca tggtgtggca
      181 acagtggctg agaagaccaa agagcaagtg acaaatgttg gaggagcagt ggtgacgggt
      241 gtgacagcag tagcccagaa gacagtggag ggagcaggga gcattgcagc agccactggc
      301 tttgtcaaaa aggaccagtt gggcaagaat gaagaaggag ccccacagga aggaattctg
      361 gaagatatgc ctgtggatcc tgacaatgag gcttatgaaa tgccttctga ggaagggtat
      421 caagactatg aacctgaagc ctaa
//
