LOCUS       sequence-464556-       12313 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Optimized construct for the expression of PEmax fused to GAG from 
            fMLV. This plasmid is involved in the production of NanoScribes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12313)
  AUTHORS   Halegua T, Risson V, Carras J, Rouyer M, Coudert L, Jacquier A, 
            Schaeffer L, Ohlmann T, Mangeot PE
  TITLE     Delivery of Prime editing in human stem cells using pseudoviral 
            NanoScribes particles.
  JOURNAL   Nat Commun. 2025 Jan 4;16(1):397. doi: 10.1038/s41467-024-55604-0.
  PUBMED    39755699
REFERENCE   2  (bases 1 to 12313)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2025-01-4"; volume: "16(1):397. doi"; pages: " 
            10.1038/s41467-024-55604-0"
COMMENT     Sequence Label: GAG-PEmax V7
FEATURES             Location/Qualifiers
     source          1..12313
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          696..1268
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     3014..3033
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3014..3032
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3082..3102
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3103..7203
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRKLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLKREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             7261..7281
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9382..9402
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9412..9438
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             9490..9516
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             9529..9555
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             9568..9594
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     primer_bind     complement(9617..9634)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9623..9847
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(9918..9934)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9918..9934)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9931..9953)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9942..9958
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9966..9972,9973..9990,9991..9996))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10011..10032
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10149..10166)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10320..10908)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10400..10419)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11079..11870,11871..11939))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11702..11721
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11940..12044)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(12123..12142)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
      661 ccagcctccg gtcgaccgat cctgagaact tcagggtgag tttggggacc cttgattgtt
      721 ctttcttttt cgctattgta aaattcatgt tatatggagg gggcaaagtt ttcagggtgt
      781 tgtttagaat gggaagatgt cccttgtatc accatggacc ctcatgataa ttttgtttct
      841 ttcactttct actctgttga caaccattgt ctcctcttat cttcttttca ttttctgtaa
      901 ctttttcgtt aaactttagc ttgcatttgt aacgaatttt taaattcact tttgtttatt
      961 tgtcagattg taagtacttt ctctaatcac ttttttttca aggcaatcag ggtatattat
     1021 attgtacttc agcacagttt tagagaacaa ttgttataat taaatgataa ggtagaatat
     1081 ttctgcatat aaattctggc tggcgtggaa atattcttat tggtagaaac aactacaccc
     1141 tggtcatcat cctgcctttc tctttatggt tacaatgata tacactgttt gagatgagga
     1201 taaaatactc tgagtccaaa ccgggcccct ctgctaacca tgttcatgcc ttcttctctt
     1261 tcctacagct cctgggcaac gtgctggttg ttgtgctgtc tcatcatttt ggcaaagaat
     1321 tcctcgaggg ccggatccga attcctgtat ttgtctgaaa acatgggcca ggctgttacc
     1381 acccccttaa gtttgacttt agaccactgg aaggatgtcg aacggacagc ccacaacctg
     1441 tcggtagagg ttagaaaaag gcgctgggtt acattctgct ctgcagaatg gccaaccttc
     1501 aacgtcggat ggccacgaga cggcactttt aacccagaca ttattacaca ggttaagatc
     1561 aaggtcttct cacctggccc acatggacat ccggatcagg tcccctacat cgtgacctgg
     1621 gaagctatag cagtagaccc ccctccctgg gtcagaccct tcgtgtaccc taaacctccc
     1681 ctctctcttc ccccttcagc cccctctctc ccacctgaac ccccactctc gaccccgccc
     1741 cagtcctccc tctatccggc tctcacttct cctttaaaca ccaaacctag gcctcaagtc
     1801 cttcctgata gcggaggacc actcattgat ctactcacgg aggaccctcc gccttaccgg
     1861 gacccagggc caccctctcc tgacgggaac ggcgatagcg gagaagtggc ccctacagaa
     1921 ggagcccctg acccttcccc aatggtatcc cgcctgcggg gaagaaaaga accccccgtg
     1981 gcggattcta ctacctctca ggcgttcccc cttcgcctgg gagggaatgg acagtatcaa
     2041 tactggccat tttcctcctc tgacctctat aactggaaaa ataacaaccc ctctttctcc
     2101 gaggacccag ctaaattgac agctttgatc gagtccgttc tccttactca tcagcccact
     2161 tgggatgact gccaacagct attagggacc ctgctgacgg gagaagaaaa acagcgagtg
     2221 ctcctagagg cccgaaaggc ggttcgaggg gaggacggac gcccaactca gctgcccaat
     2281 gacattaatg atgcttttcc cttggaacgt cccgactggg actacaacac ccaacgaggt
     2341 aggaaccacc tagtccacta tcgccagttg ctcctagcgg gtctccaaaa cgcgggcaga
     2401 agccccacca atttggccaa ggtaaaaggg ataacccagg gacctaatga gtctccctca
     2461 gcctttttag agagactcaa ggaggcctat cgcagataca ctccttatga ccctgaggac
     2521 ccagggcaag aaaccaatgt ggccatgtca ttcatctggc agtccgcccc ggatatcggg
     2581 cgaaagttag agcggttaga agatttgaag agtaagacct taggagactt agtgagggaa
     2641 gctgaaaaga tctttaataa acgagaaacc ccggaagaaa gagaggaacg tattaggaga
     2701 gaaacagagg aaaaggaaga acgccgtagg gcagaggatg tgcagagaga gaaggagagg
     2761 gaccgcagaa gacatagaga aatgagtaag ttgctggcta ctgtcgttag cgggcagaga
     2821 caggatagac agggaggaga gcgaaggagg ccccaactcg accacgacca gtgtgcctac
     2881 tgcaaagaaa agggacattg ggctagagat tgccccaaga agccaagagg accccgggga
     2941 ccacgacccc aggcctccct cctgacgcgt agttccctgt atccagccct cacacctacc
     3001 ggtgggcggc cgctaatacg actcactata gggagagccg ccaccatgaa acggacagcc
     3061 gacggaagcg agttcgagtc accaaagaag aagcggaaag tcgacaagaa gtacagcatc
     3121 ggcctggaca tcggcaccaa ctctgtgggc tgggccgtga tcaccgacga gtacaaggtg
     3181 cccagcaaga aattcaaggt gctgggcaac accgaccggc acagcatcaa gaagaacctg
     3241 atcggagccc tgctgttcga cagcggcgaa acagccgagg ccacccggct gaagagaacc
     3301 gccagaagaa gatacaccag acggaagaac cggatctgct atctgcaaga gatcttcagc
     3361 aacgagatgg ccaaggtgga cgacagcttc ttccacagac tggaagagtc cttcctggtg
     3421 gaagaggata agaagcacga gcggcacccc atcttcggca acatcgtgga cgaggtggcc
     3481 taccacgaga agtaccccac catctaccac ctgagaaaga aactggtgga cagcaccgac
     3541 aaggccgacc tgcggctgat ctatctggcc ctggcccaca tgatcaagtt ccggggccac
     3601 ttcctgatcg agggcgacct gaaccccgac aacagcgacg tggacaagct gttcatccag
     3661 ctggtgcaga cctacaacca gctgttcgag gaaaacccca tcaacgccag cggcgtggac
     3721 gccaaggcca tcctgtctgc cagactgagc aagagcagaa agctggaaaa tctgatcgcc
     3781 cagctgcccg gcgagaagaa gaatggcctg ttcggaaacc tgattgccct gagcctgggc
     3841 ctgaccccca acttcaagag caacttcgac ctggccgagg atgccaaact gcagctgagc
     3901 aaggacacct acgacgacga cctggacaac ctgctggccc agatcggcga ccagtacgcc
     3961 gacctgtttc tggccgccaa gaacctgtcc gacgccatcc tgctgagcga catcctgaga
     4021 gtgaacaccg agatcaccaa ggcccccctg agcgcctcta tgatcaagag atacgacgag
     4081 caccaccagg acctgaccct gctgaaagct ctcgtgcggc agcagctgcc tgagaagtac
     4141 aaagagattt tcttcgacca gagcaagaac ggctacgccg gctacattga cggcggagcc
     4201 agccaggaag agttctacaa gttcatcaag cccatcctgg aaaagatgga cggcaccgag
     4261 gaactgctcg tgaagctgaa gagagaggac ctgctgcgga agcagcggac cttcgacaac
     4321 ggcagcatcc cccaccagat ccacctggga gagctgcacg ccattctgcg gcggcaggaa
     4381 gatttttacc cattcctgaa ggacaaccgg gaaaagatcg agaagatcct gaccttccgc
     4441 atcccctact acgtgggccc tctggccagg ggaaacagca gattcgcctg gatgaccaga
     4501 aagagcgagg aaaccatcac cccctggaac ttcgaggaag tggtggacaa gggcgcttcc
     4561 gcccagagct tcatcgagcg gatgaccaac ttcgataaga acctgcccaa cgagaaggtg
     4621 ctgcccaagc acagcctgct gtacgagtac ttcaccgtgt ataacgagct gaccaaagtg
     4681 aaatacgtga ccgagggaat gagaaagccc gccttcctga gcggcgagca gaaaaaggcc
     4741 atcgtggacc tgctgttcaa gaccaaccgg aaagtgaccg tgaagcagct gaaagaggac
     4801 tacttcaaga aaatcgagtg cttcgactcc gtggaaatct ccggcgtgga agatcggttc
     4861 aacgcctccc tgggcacata ccacgatctg ctgaaaatta tcaaggacaa ggacttcctg
     4921 gacaatgagg aaaacgagga cattctggaa gatatcgtgc tgaccctgac actgtttgag
     4981 gacagagaga tgatcgagga acggctgaaa acctatgccc acctgttcga cgacaaagtg
     5041 atgaagcagc tgaagcggcg gagatacacc ggctggggca ggctgagccg gaagctgatc
     5101 aacggcatcc gggacaagca gtccggcaag acaatcctgg atttcctgaa gtccgacggc
     5161 ttcgccaaca gaaacttcat gcagctgatc cacgacgaca gcctgacctt taaagaggac
     5221 atccagaaag cccaggtgtc cggccagggc gatagcctgc acgagcacat tgccaatctg
     5281 gccggcagcc ccgccattaa gaagggcatc ctgcagacag tgaaggtggt ggacgagctc
     5341 gtgaaagtga tgggccggca caagcccgag aacatcgtga tcgaaatggc cagagagaac
     5401 cagaccaccc agaagggaca gaagaacagc cgcgagagaa tgaagcggat cgaagagggc
     5461 atcaaagagc tgggcagcca gatcctgaaa gaacaccccg tggaaaacac ccagctgcag
     5521 aacgagaagc tgtacctgta ctacctgcag aatgggcggg atatgtacgt ggaccaggaa
     5581 ctggacatca accggctgtc cgactacgat gtggacgcta tcgtgcctca gagctttctg
     5641 aaggacgact ccatcgacaa caaggtgctg accagaagcg acaagaaccg gggcaagagc
     5701 gacaacgtgc cctccgaaga ggtcgtgaag aagatgaaga actactggcg gcagctgctg
     5761 aacgccaagc tgattaccca gagaaagttc gacaatctga ccaaggccga gagaggcggc
     5821 ctgagcgaac tggataaggc cggcttcatc aagagacagc tggtggaaac ccggcagatc
     5881 acaaagcacg tggcacagat cctggactcc cggatgaaca ctaagtacga cgagaatgac
     5941 aagctgatcc gggaagtgaa agtgatcacc ctgaagtcca agctggtgtc cgatttccgg
     6001 aaggatttcc agttttacaa agtgcgcgag atcaacaact accaccacgc ccacgacgcc
     6061 tacctgaacg ccgtcgtggg aaccgccctg atcaaaaagt accctaagct ggaaagcgag
     6121 ttcgtgtacg gcgactacaa ggtgtacgac gtgcggaaga tgatcgccaa gagcgagcag
     6181 gaaatcggca aggctaccgc caagtacttc ttctacagca acatcatgaa ctttttcaag
     6241 accgagatta ccctggccaa cggcgagatc cggaagcggc ctctgatcga gacaaacggc
     6301 gaaaccgggg agatcgtgtg ggataagggc cgggattttg ccaccgtgcg gaaagtgctg
     6361 agcatgcccc aagtgaatat cgtgaaaaag accgaggtgc agacaggcgg cttcagcaaa
     6421 gagtctatcc tgcccaagag gaacagcgat aagctgatcg ccagaaagaa ggactgggac
     6481 cctaagaagt acggcggctt cgacagcccc accgtggcct attctgtgct ggtggtggcc
     6541 aaagtggaaa agggcaagtc caagaaactg aagagtgtga aagagctgct ggggatcacc
     6601 atcatggaaa gaagcagctt cgagaagaat cccatcgact ttctggaagc caagggctac
     6661 aaagaagtga aaaaggacct gatcatcaag ctgcctaagt actccctgtt cgagctggaa
     6721 aacggccgga agagaatgct ggcctctgcc ggcgaactgc agaagggaaa cgaactggcc
     6781 ctgccctcca aatatgtgaa cttcctgtac ctggccagcc actatgagaa gctgaagggc
     6841 tcccccgagg ataatgagca gaaacagctg tttgtggaac agcacaagca ctacctggac
     6901 gagatcatcg agcagatcag cgagttctcc aagagagtga tcctggccga cgctaatctg
     6961 gacaaagtgc tgtccgccta caacaagcac cgggataagc ccatcagaga gcaggccgag
     7021 aatatcatcc acctgtttac cctgaccaat ctgggagccc ctgccgcctt caagtacttt
     7081 gacaccacca tcgaccggaa gaggtacacc agcaccaaag aggtgctgga cgccaccctg
     7141 atccaccaga gcatcaccgg cctgtacgag acacggatcg acctgtctca gctgggaggt
     7201 gactccggcg gaagctctgg tggcagcaag cggaccgccg acggctctga attcgagagc
     7261 cctaagaaga aaagaaaggt gagcggaggc tctagcggcg gaagcaccct gaacattgaa
     7321 gacgagtata gactgcatga aacaagcaag gaacccgacg tgtccctggg ctccacctgg
     7381 ctgtccgact ttccccaggc ctgggccgag acaggaggaa tgggcctggc cgtgcggcag
     7441 gcacccctga tcatccctct gaaggccacc tctacacccg tgagcatcaa gcagtaccct
     7501 atgtctcagg aggccagact gggcatcaag cctcacatcc agaggctgct ggaccagggc
     7561 atcctggtgc catgccagag cccctggaac acaccactgc tgcccgtgaa gaagccaggc
     7621 accaatgact atagacccgt gcaggatctg agagaggtga acaagagggt ggaggatatc
     7681 caccccaccg tgcccaaccc ttacaatctg ctgtccggcc tgcccccttc tcaccagtgg
     7741 tatacagtgc tggacctgaa ggatgccttc ttttgtctga gactgcaccc taccagccag
     7801 ccactgttcg cctttgagtg gagggaccct gagatgggca tctctggcca gctgacctgg
     7861 acacgcctgc ctcagggctt caagaatagc ccaacactgt ttaacgaggc cctgcaccgc
     7921 gacctggcag atttccggat ccagcaccca gatctgatcc tgctgcagta cgtggacgat
     7981 ctgctgctgg ccgccaccag cgagctggat tgccagcagg gaacacgcgc cctgctgcag
     8041 accctgggaa acctgggata tagggcatcc gccaagaagg cccagatctg tcagaagcag
     8101 gtgaagtacc tgggctatct gctgaaggag ggccagagat ggctgacaga ggccaggaag
     8161 gagacagtga tgggccagcc aacacccaag accccaagac agctgaggga gttcctgggc
     8221 aaagcaggat tttgcaggct gttcatccca ggattcgcag agatggcagc acctctgtac
     8281 ccactgacca agccgggcac cctgtttaat tggggccctg accagcagaa ggcctatcag
     8341 gagatcaagc aggccctgct gacagcacca gccctgggcc tgccagacct gaccaagcct
     8401 ttcgagctgt ttgtggatga gaagcagggc tacgccaagg gcgtgctgac ccagaagctg
     8461 ggaccatgga gacggcccgt ggcctatctg tccaagaagc tggacccagt ggcagcagga
     8521 tggccaccat gcctgaggat ggtggcagca atcgccgtgc tgacaaagga tgccggcaag
     8581 ctgaccatgg gacagccact ggtcatcctg gcaccacacg cagtggaggc cctggtgaag
     8641 cagcctccag atcgctggct gtctaacgcc cggatgacac actaccaggc cctgctgctg
     8701 gacaccgatc gcgtgcagtt tggccctgtg gtggccctga atccagccac cctgctgcct
     8761 ctgccagagg agggcctgca gcacaactgt ctggacatcc tggcagaggc acacggaaca
     8821 aggccagacc tgaccgatca gcccctgcct gacgccgatc acacatggta taccgatgga
     8881 agctccctgc tgcaggaggg ccagaggaag gcaggagcag cagtgaccac agagacagaa
     8941 gtgatctggg ccaaggccct gccagcaggc acatccgccc agcgggccga gctgatcgcc
     9001 ctgacccagg ccctgaagat ggccgagggc aagaagctga acgtgtacac agactccaga
     9061 tatgccttcg ccaccgcaca catccacgga gagatctaca ggcgccgggg ctggctgacc
     9121 tctgagggca aggagatcaa gaacaaggat gagatcctgg ccctgctgaa ggccctgttt
     9181 ctgcccaagc ggctgagcat catccactgt cctggacacc agaagggaca ctccgccgag
     9241 gcaaggggca atcggatggc cgaccaggcc gccagaaagg ctgctattac tgaaactccc
     9301 gacacttcca ctctgctgat tgaaaactcc tccccttctg gcggctcaaa aagaaccgcc
     9361 gacggcagcg aattcgagtc tcccaagaag aagaggaaag tcggctctgg ccctgccgct
     9421 aagagagtga agctggacaa gcttacgcgt agttccctgt atccagccct cacacctacc
     9481 ggttctagac tgcctccact tgaaagactg acactgggat cattacaatt acctccttta
     9541 gaacgattaa cactcggttc actacagctt ccgcctcttg agagattgac attataagtt
     9601 taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     9661 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     9721 gagaaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     9781 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
     9841 tctatggctt ctgaggcgga aagaaccagc tggggctcga taccgtcgac ctctagctag
     9901 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     9961 ccacacaaca tacgagccgg aagcataaag tgtaaagcct agggtgccta atgagtgagc
    10021 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
    10081 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
    10141 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
    10201 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
    10261 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
    10321 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
    10381 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
    10441 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
    10501 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
    10561 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
    10621 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
    10681 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
    10741 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
    10801 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
    10861 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
    10921 atcttttcta cggggtctga cactcagtgg aacgaaaact cacgttaagg gattttggtc
    10981 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
    11041 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
    11101 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
    11161 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
    11221 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
    11281 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
    11341 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
    11401 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
    11461 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
    11521 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
    11581 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
    11641 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
    11701 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
    11761 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
    11821 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
    11881 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
    11941 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
    12001 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
    12061 gtgccacctg acgtcgacgg atcgggagat cgatctcccg atcccctagg gtcgactctc
    12121 agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg
    12181 gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac
    12241 aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc
    12301 agatatacgc gtt
//
