LOCUS       sequence-464600-       10199 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast CRISPR plasmid targeting the natMX and hphMX cassettes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10199)
  AUTHORS   Soreanu I, Hendler A, Dahan D, Dovrat D, Aharoni A
  TITLE     Marker-free genetic manipulations in yeast using CRISPR/CAS9 system.
  JOURNAL   Curr Genet. 2018 Oct;64(5):1129-1139. doi: 
            10.1007/s00294-018-0831-y. Epub 2018 Apr 6.
  PUBMED    29626221
REFERENCE   2  (bases 1 to 10199)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s00294-018-0831-y"; journalName: "Curr Genet"; date: 
            "2018-10"; volume: "64"; issue: "5"; pages: "1129-1139"
COMMENT     Sequence Label: pCY5.1nh
FEATURES             Location/Qualifiers
     source          1..10199
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_RNA        225..300
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        436..779
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     terminator      1598..1795
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     promoter        1846..1950
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1951..2019,2020..2811)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2169..2188)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     misc_RNA        3138..3213
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             4009..8112
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             4009..8112
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             8122..8142
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8158..8181
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     terminator      8185..8374
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     rep_origin      complement(8431..9019)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8511..8530)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     rep_origin      9041..10023
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(10004..10051)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
ORIGIN
        1 gtatatgtgt tatgtagtat actctttctt caacaattaa atactctcgg tagccaagtt
       61 ggtttaaggc gcaagactgt aatttatcac tacgaaatct tgagatcggg cgttcgactc
      121 gcccccggga gagatggccg gcatggtccc agcctcctcg ctggcgccgg ctgggcaaca
      181 ccttcgggtg gcgaatggga ctttgacctg atgcagctct cggagtttta gagctagaaa
      241 tagcaagtta aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc
      301 tttttttatt ttttgtcact attgttatgt aaaatgccac ctctgacagt atggaacgca
      361 aacttctgtc tagtggatat aggtctagag atctaccggt agcttgcctt gtccccgccg
      421 ggtcacccgg ccagcgacat ggaggcccag aataccctcc ttgacagtct tgacgtgcgc
      481 agctcagggg catgatgtga ctgtcgcccg tacatttagc ccatacatcc ccatgtataa
      541 tcatttgcat ccatacattt tgatggccgc acggcgcgaa gcaaaaatta cggctcctcg
      601 ctgcggacct gcgagcaggg aaacgctccc ctcacagacg cgttgaattg tccccacgcc
      661 gcgcccctgt agagaaatat aaaaggttag gatttgccac tgaggttctt ctttcatata
      721 cttcctttta aaatcttgct aggatacagt tctcacatca catccgaaca taaacaacca
      781 tgacagtcaa cactaagacc tatagtgaga gagcagaaac tcatgcctca ccagtagcac
      841 aacgattatt tcgattaatg gaactgaaga aaaccaattt atgtgcatca attgatgttg
      901 ataccactaa ggaattcctt gaattaattg ataaattggg tccttatgta tgcttaatca
      961 agactcatat tgatataatc aatgattttt cctatgaatc cactattgaa ccattattag
     1021 aactttcacg taaacatcaa tttatgattt ttgaagatag aaaatttgct gatattggta
     1081 ataccgtgaa gaaacaatat attggtggag tttataaaat tagtagttgg gcagatatta
     1141 ctaatgctca tggtgtcact gggaatggag tagttgaagg attaaaacag ggagctaaag
     1201 aaaccaccac caaccaagag ccaagagggt tattgatgtt agctgaatta tcatcagtgg
     1261 gatcattagc atatggagaa tattctcaaa aaactgttga aattgctaaa tccgataagg
     1321 aatttgttat tggatttatt gcccaacgtg atatgggtgg acaagaagaa ggatttgatt
     1381 ggcttattat gacacctgga gttggattag atgataaagg tgatggatta ggacaacaat
     1441 atagaactgt tgatgaagtt gttagcactg gaactgatat tatcattgtt ggtagaggat
     1501 tgtttggtaa aggaagagat ccagatattg aaggtaaaag gtatagagat gctggttgga
     1561 atgcttattt gaaaaagact ggccaattat aatcagtact gacaataaaa agattcttgt
     1621 tttcaagaac ttgtcatttg tatagttttt ttatattgta gttgttctat tttaatcaaa
     1681 tgttagcgtg atttatattt tttttcgcct cgacatcatc tgcccagatg cgaagttaag
     1741 tgcgcagaaa gtaatatcat gcgtcaatcg tatgtgaatg ctggtcgcta tactgctgtc
     1801 gattcgatac taacgccgcc atccagtgtc gaaaacggcg gccgccgcgg aacccctatt
     1861 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     1921 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     1981 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     2041 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     2101 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     2161 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     2221 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     2281 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     2341 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     2401 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     2461 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     2521 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     2581 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     2641 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     2701 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     2761 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     2821 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     2881 taggtgaaga tcctttttga taatcttaat taagtatatg tgttatgtag tatactcttt
     2941 cttcaacaat taaatactct cggtagccaa gttggtttaa ggcgcaagac tgtaatttat
     3001 cactacgaaa tcttgagatc gggcgttcga ctcgcccccg ggagagatgg ccggcatggt
     3061 cccagcctcc tcgctggcgc cggctgggca acaccttcgg gtggcgaatg ggactttgta
     3121 ccgcaccagt gtcccgggtt ttagagctag aaatagcaag ttaaaataag gctagtccgt
     3181 tatcaacttg aaaaagtggc accgagtcgg tgcttttttt attttttgtc actattgtta
     3241 tgtaaaatgc cacctctgac agtatggaac gcaaacttct gtctagtgga tactcgagaa
     3301 cccttaatag tcgaacaaga agcaggcaaa gtttagagca ctgcccctcc gcactcaaaa
     3361 aagaaaaaac taggaggaaa ataaaattct caaccacaca aacacataaa cacatacaaa
     3421 tacaaataca agcttattta cttgacatcg cgcgatcttc cactattcag cgccgtccgc
     3481 cctctctcgt gttttttgtt tacgcgacaa ctatgcgaaa tccggagcaa cgggcaaccg
     3541 tttggggaaa gaccacaccc acgcgcgatc gccatggcaa cgaggtcgca cacgccccac
     3601 acccagacct ccctgcgagc gggcatgggt acaatgtccc cgttgccaca gacaccactt
     3661 cgtagcacag cgcagagcgt agcgtgttgt tgctgctgac aaaagaaaat ttttcttagc
     3721 aaagcaaagg aggggaagca cgggcagata gcaccgtacc atacccttgg aaactcgaaa
     3781 tgaacgaagc aggaaatgag agaatgagag ttttgtaggt atatatagcg gtagtgtttg
     3841 cgcgttacca tcatcttctg gatctatcta ttgttctttt cctcatcact ttcccctttt
     3901 tcgctcttct tcttgtcttt tatttctttc ttttttttaa ttgttccctc gattggctat
     3961 ctaccaaaga atccaaactt aatacacgta tttatttgtc caattaccat ggataagaaa
     4021 tactcaatag gcttagatat cggcacaaat agcgtcggat gggcggtgat cactgatgaa
     4081 tataaggttc cgtctaaaaa gttcaaggtt ctgggaaata cagaccgcca cagtatcaaa
     4141 aaaaatctta taggggctct tttatttgac agtggagaga cagcggaagc gactcgtctc
     4201 aaacggacag ctcgtagaag gtatacacgt cggaagaatc gtatttgtta tctacaggag
     4261 attttttcaa atgagatggc gaaagtagat gatagtttct ttcatcgact tgaagagtct
     4321 tttttggtgg aagaagacaa gaagcatgaa cgtcatccta tttttggaaa tatagtagat
     4381 gaagttgctt atcatgagaa atatccaact atctatcatc tgcgaaaaaa attggtagat
     4441 tctactgata aagcggattt gcgcttaatc tatttggcct tagcgcatat gattaagttt
     4501 cgtggtcatt ttttgattga gggagattta aatcctgata atagtgatgt ggacaaacta
     4561 tttatccagt tggtacaaac ctacaatcaa ttatttgaag aaaaccctat taacgcaagt
     4621 ggagtagatg ctaaagcgat tctttctgca cgattgagta aatcaagacg attagaaaat
     4681 ctcattgctc agctccccgg tgagaagaaa aatggcttat ttgggaatct cattgctttg
     4741 tcattgggtt tgacccctaa ttttaaatca aattttgatt tggcagaaga tgctaaatta
     4801 cagctttcaa aagatactta cgatgatgat ttagataatt tattggcgca aattggagat
     4861 caatatgctg atttgttttt ggcagctaag aatttatcag atgctatttt actttcagat
     4921 atcctaagag taaatactga aataactaag gctcccctat cagcttcaat gattaaacgc
     4981 tacgatgaac atcatcaaga cttgactctt ttaaaagctt tagttcgaca acaacttcca
     5041 gaaaagtata aagaaatctt ttttgatcaa tcaaaaaacg gatatgcagg ttatattgat
     5101 gggggagcta gccaagaaga attttataaa tttatcaaac caattttaga aaaaatggat
     5161 ggtactgagg aattattggt gaaactaaat cgtgaagatt tgctgcgcaa gcaacggacc
     5221 tttgacaacg gctctattcc ccatcaaatt cacttgggtg agctgcatgc tattttgaga
     5281 agacaagaag acttttatcc atttttaaaa gacaatcgtg agaagattga aaaaatcttg
     5341 acttttcgaa ttccttatta tgttggtcca ttggcgcgtg gcaatagtcg ttttgcatgg
     5401 atgactcgga agtctgaaga aacaattacc ccatggaatt ttgaagaagt tgtcgataaa
     5461 ggtgcttcag ctcaatcatt tattgaacgc atgacaaact ttgataaaaa tcttccaaat
     5521 gaaaaagtac taccaaaaca tagtttgctt tatgagtatt ttacggttta taacgaattg
     5581 acaaaggtca aatatgttac tgaaggaatg cgaaaaccag catttctttc aggtgaacag
     5641 aagaaagcca ttgttgattt actcttcaaa acaaatcgaa aagtaaccgt taagcaatta
     5701 aaagaagatt atttcaaaaa aatagaatgt tttgatagtg ttgaaatttc aggagttgaa
     5761 gatagattta atgcttcatt aggtacctac catgatttgc taaaaattat taaagataaa
     5821 gattttttgg ataatgaaga aaatgaagat atcttagagg atattgtttt aacattgacc
     5881 ttatttgaag atagggagat gattgaggaa agacttaaaa catatgctca cctctttgat
     5941 gataaggtga tgaaacagct taaacgtcgc cgttatactg gttggggacg tttgtctcga
     6001 aaattgatta atggtattag ggataagcaa tctggcaaaa caatattaga ttttttgaaa
     6061 tcagatggtt ttgccaatcg caattttatg cagctgatcc atgatgatag tttgacattt
     6121 aaagaagaca ttcaaaaagc acaagtgtct ggacaaggcg atagtttaca tgaacatatt
     6181 gcaaatttag ctggtagccc tgctattaaa aaaggtattt tacagactgt aaaagttgtt
     6241 gatgaattgg tcaaagtaat ggggcggcat aagccagaaa atatcgttat tgaaatggca
     6301 cgtgaaaatc agacaactca aaagggccag aaaaattcgc gagagcgtat gaaacgaatc
     6361 gaagaaggta tcaaagaatt aggaagtcag attcttaaag agcatcctgt tgaaaatact
     6421 caattgcaaa atgaaaagct ctatctctat tatctccaaa atggaagaga catgtatgtg
     6481 gaccaagaat tagatattaa tcgtttaagt gattatgatg tcgatcacat tgttccacaa
     6541 agtttcctta aagacgattc aatagacaat aaggtcttaa cgcgttctga taaaaatcgt
     6601 ggtaaatcgg ataacgttcc aagtgaagaa gtagtcaaaa agatgaaaaa ctattggaga
     6661 caacttctaa acgccaagtt aatcactcaa cgtaagtttg ataatttaac gaaagctgaa
     6721 cgtggaggtt tgagtgaact tgataaagct ggttttatca aacgccaatt ggttgaaact
     6781 cgccaaatca ctaagcatgt ggcacaaatt ttggatagtc gcatgaatac taaatacgat
     6841 gaaaatgata aacttattcg agaggttaaa gtgattacct taaaatctaa attagtttct
     6901 gacttccgaa aagatttcca attctataaa gtacgtgaga ttaacaatta ccatcatgcc
     6961 catgatgcgt atctaaatgc cgtcgttgga actgctttga ttaagaaata tccaaaactt
     7021 gaatcggagt ttgtctatgg tgattataaa gtttatgatg ttcgtaaaat gattgctaag
     7081 tctgagcaag aaataggcaa agcaaccgca aaatatttct tttactctaa tatcatgaac
     7141 ttcttcaaaa cagaaattac acttgcaaat ggagagattc gcaaacgccc tctaatcgaa
     7201 actaatgggg aaactggaga aattgtctgg gataaagggc gagattttgc cacagtgcgc
     7261 aaagtattgt ccatgcccca agtcaatatt gtcaagaaaa cagaagtaca gacaggcgga
     7321 ttctccaagg agtcaatttt accaaaaaga aattcggaca agcttattgc tcgtaaaaaa
     7381 gactgggatc caaaaaaata tggtggtttt gatagtccaa cggtagctta ttcagtccta
     7441 gtggttgcta aggtggaaaa agggaaatcg aagaagttaa aatccgttaa agagttacta
     7501 gggatcacaa ttatggaaag aagttccttt gaaaaaaatc cgattgactt tttagaagct
     7561 aaaggatata aggaagttaa aaaagactta atcattaaac tacctaaata tagtcttttt
     7621 gagttagaaa acggtcgtaa acggatgctg gctagtgccg gagaattaca aaaaggaaat
     7681 gagctggctc tgccaagcaa atatgtgaat tttttatatt tagctagtca ttatgaaaag
     7741 ttgaagggta gtccagaaga taacgaacaa aaacaattgt ttgtggagca gcataagcat
     7801 tatttagatg agattattga gcaaatcagt gaattttcta agcgtgttat tttagcagat
     7861 gccaatttag ataaagttct tagtgcatat aacaaacata gagacaaacc aatacgtgaa
     7921 caagcagaaa atattattca tttatttacg ttgacgaatc ttggagctcc cgctgctttt
     7981 aaatattttg atacaacaat tgatcgtaaa cgatatacgt ctacaaaaga agttttagat
     8041 gccactctta tccatcaatc catcactggt ctttatgaaa cacgcattga tttgagtcag
     8101 ctaggaggtg acggtggagg gccaaaaaag aaaagaaaag ttgaagatgc ttctgctcat
     8161 catcaccatc accatcacca ttaaatccgc tctaaccgaa aaggaaggag ttagacaacc
     8221 tgaagtctag gtccctattt atttttttat agttatgtta gtattaagaa cgttatttat
     8281 atttcaaatt tttctttttt ttctgtacag acgcgtgtac gcatgtaaca ttatactgaa
     8341 aaccttgctt gagaaggttt tgggacgctc gaagtgagca aaaggccagc aaaaggccag
     8401 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     8461 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     8521 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     8581 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     8641 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     8701 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     8761 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     8821 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     8881 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     8941 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     9001 cagaaaaaaa ggatctcaag aagatccttt gatcttttct accgaagcat ctgtgcttca
     9061 ttttgtagaa caaaaatgca acgcgagagc gctaattttt caaacaaaga atctgagctg
     9121 catttttaca gaacagaaat gcaacgcgaa agcgctattt taccaacgaa gaatctgtgc
     9181 ttcatttttg taaaacaaaa atgcaacgcg agagcgctaa tttttcaaac aaagaatctg
     9241 agctgcattt ttacagaaca gaaatgcaac gcgagagcgc tattttacca acaaagaatc
     9301 tatacttctt ttttgttcta caaaaatgca tcccgagagc gctatttttc taacaaagca
     9361 tcttagatta ctttttttct cctttgtgcg ctctataatg cagtctcttg ataacttttt
     9421 gcactgtagg tccgttaagg ttagaagaag gctactttgg tgtctatttt ctcttccata
     9481 aaaaaagcct gactccactt cccgcgttta ctgattacta gcgaagctgc gggtgcattt
     9541 tttcaagata aaggcatccc cgattatatt ctataccgat gtggattgcg catactttgt
     9601 gaacagaaag tgatagcgtt gatgattctt cattggtcag aaaattatga acggtttctt
     9661 ctattttgtc tctatatact acgtatagga aatgtttaca ttttcgtatt gttttcgatt
     9721 cactctatga atagttctta ctacaatttt tttgtctaaa gagtaatact agagataaac
     9781 ataaaaaatg tagaggtcga gtttagatgc aagttcaagg agcgaaaggt ggatgggtag
     9841 gttatatagg gatatagcac agagatatat agcaaagaga tacttttgag caatgtttgt
     9901 ggaagcggta ttcgcaatat tttagtagcc cgttacagtc cggtgcgttt ttggtttttt
     9961 gaaagtgcgt cttcagagcg cttttggttt tcaaaagcgc tctgaagttc ctatactttc
    10021 tagagaatag gaacttcgga ataggaactt caaagcgttt ccgaaaacga gcgcttccga
    10081 aaatgcaacg cgagctgcgc acatacagct cactgttcac gtcgcaccta tatctgcgtg
    10141 ttgcctgtat atatatatac atgagaagaa cggcatagtg cgtgtttatg cttaaatgc
//
