LOCUS       sequence-464601-       10135 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast CRISPR plasmid targeting the kanMX cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10135)
  AUTHORS   Soreanu I, Hendler A, Dahan D, Dovrat D, Aharoni A
  TITLE     Marker-free genetic manipulations in yeast using CRISPR/CAS9 system.
  JOURNAL   Curr Genet. 2018 Oct;64(5):1129-1139. doi: 
            10.1007/s00294-018-0831-y. Epub 2018 Apr 6.
  PUBMED    29626221
REFERENCE   2  (bases 1 to 10135)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s00294-018-0831-y"; journalName: "Curr Genet"; date: 
            "2018-10"; volume: "64"; issue: "5"; pages: "1129-1139"
COMMENT     Sequence Label: pCY5k
FEATURES             Location/Qualifiers
     source          1..10135
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_RNA        225..300
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        400..615
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             616..1419
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     primer_bind     complement(1687..1708)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1796..1900
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1901..1969,1970..2761)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2119..2138)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     misc_RNA        3074..3149
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             3945..8048
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             3945..8048
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             8058..8078
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8094..8117
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     terminator      8121..8310
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     rep_origin      complement(8367..8955)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8447..8466)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     rep_origin      8977..9959
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(9940..9987)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
ORIGIN
        1 gtatatgtgt tatgtagtat actctttctt caacaattaa atactctcgg tagccaagtt
       61 ggtttaaggc gcaagactgt aatttatcac tacgaaatct tgagatcggg cgttcgactc
      121 gcccccggga gagatggccg gcatggtccc agcctcctcg ctggcgccgg ctgggcaaca
      181 ccttcgggtg gcgaatggga cttttgtttt gccggggatc gcaggtttta gagctagaaa
      241 tagcaagtta aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc
      301 tttttttatt ttttgtcact attgttatgt aaaatgccac ctctgacagt atggaacgca
      361 aacttctgtc tagtggatat aggtctagag atctaccggt tcaattcatc attttttttt
      421 tattcttttt tttgatttcg gtttccttga aatttttttg attcggtaat ctccgaacag
      481 aaggaagaac gaaggaagga gcacagactt agattggtat atatacgcat atgtagtgtt
      541 gaagaaacat gaaattgccc agtattctta acccaactgc acagaacaaa aacctgcagg
      601 aaacgaagat aaatcatgtc gaaagctaca tataaggaac gtgctgctac tcatcctagt
      661 cctgttgctg ccaagctatt taatatcatg cacgaaaagc aaacaaactt gtgtgcttca
      721 ttggatgttc gtaccaccaa ggaattactg gagttagttg aagcattagg tcccaaaatt
      781 tgtttactaa aaacacatgt ggatatcttg actgattttt ccatggaggg cacagttaag
      841 ccgctaaagg cattatccgc caagtacaat tttttactct tcgaagacag aaaatttgct
      901 gacattggta atacagtcaa attgcagtac tctgcgggtg tatacagaat agcagaatgg
      961 gcagacatta cgaatgcaca cggtgtggtg ggcccaggta ttgttagcgg tttgaagcag
     1021 gcggcagaag aagtaacaaa ggaacctaga ggccttttga tgttagcaga attgtcatgc
     1081 aagggctccc tatctactgg agaatatact aagggtactg ttgacattgc gaagagcgac
     1141 aaagattttg ttatcggctt tattgctcaa agagacatgg gtggaagaga tgaaggttac
     1201 gattggttga ttatgacacc cggtgtgggt ttagatgaca agggagacgc attgggtcaa
     1261 cagtatagaa ccgtggatga tgtggtctct acaggatctg acattattat tgttggaaga
     1321 ggactatttg caaagggaag ggatgctaag gtagagggtg aacgttacag aaaagcaggc
     1381 tgggaagcat atttgagaag atgcggccag caaaactaaa aaactgtatt ataagtaaat
     1441 gcatgtatac taaactcaca aattagagct tcaatttaat tatatcagtt attaccctat
     1501 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcagga aattgtaagc
     1561 gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa
     1621 taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt
     1681 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg
     1741 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcaccgcg gccgccgcgg
     1801 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     1861 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     1921 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     1981 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     2041 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     2101 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     2161 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     2221 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     2281 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     2341 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     2401 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     2461 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     2521 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     2581 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     2641 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     2701 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     2761 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     2821 taaaaggatc taggtgaaga tcctttttga taatcttaat taagtatatg tgttatgtag
     2881 tatactcttt cttcaacaat taaatactct cggtagccaa gttggtttaa ggcgcaagac
     2941 tgtaatttat cactacgaaa tcttgagatc gggcgttcga ctcgcccccg ggagagatgg
     3001 ccggcatggt cccagcctcc tcgctggcgc cggctgggca acaccttcgg gtggcgaatg
     3061 ggactttgtt aacgttttag agctagaaat agcaagttaa aataaggcta gtccgttatc
     3121 aacttgaaaa agtggcaccg agtcggtgct ttttttattt tttgtcacta ttgttatgta
     3181 aaatgccacc tctgacagta tggaacgcaa acttctgtct agtggatact cgagaaccct
     3241 taatagtcga acaagaagca ggcaaagttt agagcactgc ccctccgcac tcaaaaaaga
     3301 aaaaactagg aggaaaataa aattctcaac cacacaaaca cataaacaca tacaaataca
     3361 aatacaagct tatttacttg acatcgcgcg atcttccact attcagcgcc gtccgccctc
     3421 tctcgtgttt tttgtttacg cgacaactat gcgaaatccg gagcaacggg caaccgtttg
     3481 gggaaagacc acacccacgc gcgatcgcca tggcaacgag gtcgcacacg ccccacaccc
     3541 agacctccct gcgagcgggc atgggtacaa tgtccccgtt gccacagaca ccacttcgta
     3601 gcacagcgca gagcgtagcg tgttgttgct gctgacaaaa gaaaattttt cttagcaaag
     3661 caaaggaggg gaagcacggg cagatagcac cgtaccatac ccttggaaac tcgaaatgaa
     3721 cgaagcagga aatgagagaa tgagagtttt gtaggtatat atagcggtag tgtttgcgcg
     3781 ttaccatcat cttctggatc tatctattgt tcttttcctc atcactttcc cctttttcgc
     3841 tcttcttctt gtcttttatt tctttctttt ttttaattgt tccctcgatt ggctatctac
     3901 caaagaatcc aaacttaata cacgtattta tttgtccaat taccatggat aagaaatact
     3961 caataggctt agatatcggc acaaatagcg tcggatgggc ggtgatcact gatgaatata
     4021 aggttccgtc taaaaagttc aaggttctgg gaaatacaga ccgccacagt atcaaaaaaa
     4081 atcttatagg ggctctttta tttgacagtg gagagacagc ggaagcgact cgtctcaaac
     4141 ggacagctcg tagaaggtat acacgtcgga agaatcgtat ttgttatcta caggagattt
     4201 tttcaaatga gatggcgaaa gtagatgata gtttctttca tcgacttgaa gagtcttttt
     4261 tggtggaaga agacaagaag catgaacgtc atcctatttt tggaaatata gtagatgaag
     4321 ttgcttatca tgagaaatat ccaactatct atcatctgcg aaaaaaattg gtagattcta
     4381 ctgataaagc ggatttgcgc ttaatctatt tggccttagc gcatatgatt aagtttcgtg
     4441 gtcatttttt gattgaggga gatttaaatc ctgataatag tgatgtggac aaactattta
     4501 tccagttggt acaaacctac aatcaattat ttgaagaaaa ccctattaac gcaagtggag
     4561 tagatgctaa agcgattctt tctgcacgat tgagtaaatc aagacgatta gaaaatctca
     4621 ttgctcagct ccccggtgag aagaaaaatg gcttatttgg gaatctcatt gctttgtcat
     4681 tgggtttgac ccctaatttt aaatcaaatt ttgatttggc agaagatgct aaattacagc
     4741 tttcaaaaga tacttacgat gatgatttag ataatttatt ggcgcaaatt ggagatcaat
     4801 atgctgattt gtttttggca gctaagaatt tatcagatgc tattttactt tcagatatcc
     4861 taagagtaaa tactgaaata actaaggctc ccctatcagc ttcaatgatt aaacgctacg
     4921 atgaacatca tcaagacttg actcttttaa aagctttagt tcgacaacaa cttccagaaa
     4981 agtataaaga aatctttttt gatcaatcaa aaaacggata tgcaggttat attgatgggg
     5041 gagctagcca agaagaattt tataaattta tcaaaccaat tttagaaaaa atggatggta
     5101 ctgaggaatt attggtgaaa ctaaatcgtg aagatttgct gcgcaagcaa cggacctttg
     5161 acaacggctc tattccccat caaattcact tgggtgagct gcatgctatt ttgagaagac
     5221 aagaagactt ttatccattt ttaaaagaca atcgtgagaa gattgaaaaa atcttgactt
     5281 ttcgaattcc ttattatgtt ggtccattgg cgcgtggcaa tagtcgtttt gcatggatga
     5341 ctcggaagtc tgaagaaaca attaccccat ggaattttga agaagttgtc gataaaggtg
     5401 cttcagctca atcatttatt gaacgcatga caaactttga taaaaatctt ccaaatgaaa
     5461 aagtactacc aaaacatagt ttgctttatg agtattttac ggtttataac gaattgacaa
     5521 aggtcaaata tgttactgaa ggaatgcgaa aaccagcatt tctttcaggt gaacagaaga
     5581 aagccattgt tgatttactc ttcaaaacaa atcgaaaagt aaccgttaag caattaaaag
     5641 aagattattt caaaaaaata gaatgttttg atagtgttga aatttcagga gttgaagata
     5701 gatttaatgc ttcattaggt acctaccatg atttgctaaa aattattaaa gataaagatt
     5761 ttttggataa tgaagaaaat gaagatatct tagaggatat tgttttaaca ttgaccttat
     5821 ttgaagatag ggagatgatt gaggaaagac ttaaaacata tgctcacctc tttgatgata
     5881 aggtgatgaa acagcttaaa cgtcgccgtt atactggttg gggacgtttg tctcgaaaat
     5941 tgattaatgg tattagggat aagcaatctg gcaaaacaat attagatttt ttgaaatcag
     6001 atggttttgc caatcgcaat tttatgcagc tgatccatga tgatagtttg acatttaaag
     6061 aagacattca aaaagcacaa gtgtctggac aaggcgatag tttacatgaa catattgcaa
     6121 atttagctgg tagccctgct attaaaaaag gtattttaca gactgtaaaa gttgttgatg
     6181 aattggtcaa agtaatgggg cggcataagc cagaaaatat cgttattgaa atggcacgtg
     6241 aaaatcagac aactcaaaag ggccagaaaa attcgcgaga gcgtatgaaa cgaatcgaag
     6301 aaggtatcaa agaattagga agtcagattc ttaaagagca tcctgttgaa aatactcaat
     6361 tgcaaaatga aaagctctat ctctattatc tccaaaatgg aagagacatg tatgtggacc
     6421 aagaattaga tattaatcgt ttaagtgatt atgatgtcga tcacattgtt ccacaaagtt
     6481 tccttaaaga cgattcaata gacaataagg tcttaacgcg ttctgataaa aatcgtggta
     6541 aatcggataa cgttccaagt gaagaagtag tcaaaaagat gaaaaactat tggagacaac
     6601 ttctaaacgc caagttaatc actcaacgta agtttgataa tttaacgaaa gctgaacgtg
     6661 gaggtttgag tgaacttgat aaagctggtt ttatcaaacg ccaattggtt gaaactcgcc
     6721 aaatcactaa gcatgtggca caaattttgg atagtcgcat gaatactaaa tacgatgaaa
     6781 atgataaact tattcgagag gttaaagtga ttaccttaaa atctaaatta gtttctgact
     6841 tccgaaaaga tttccaattc tataaagtac gtgagattaa caattaccat catgcccatg
     6901 atgcgtatct aaatgccgtc gttggaactg ctttgattaa gaaatatcca aaacttgaat
     6961 cggagtttgt ctatggtgat tataaagttt atgatgttcg taaaatgatt gctaagtctg
     7021 agcaagaaat aggcaaagca accgcaaaat atttctttta ctctaatatc atgaacttct
     7081 tcaaaacaga aattacactt gcaaatggag agattcgcaa acgccctcta atcgaaacta
     7141 atggggaaac tggagaaatt gtctgggata aagggcgaga ttttgccaca gtgcgcaaag
     7201 tattgtccat gccccaagtc aatattgtca agaaaacaga agtacagaca ggcggattct
     7261 ccaaggagtc aattttacca aaaagaaatt cggacaagct tattgctcgt aaaaaagact
     7321 gggatccaaa aaaatatggt ggttttgata gtccaacggt agcttattca gtcctagtgg
     7381 ttgctaaggt ggaaaaaggg aaatcgaaga agttaaaatc cgttaaagag ttactaggga
     7441 tcacaattat ggaaagaagt tcctttgaaa aaaatccgat tgacttttta gaagctaaag
     7501 gatataagga agttaaaaaa gacttaatca ttaaactacc taaatatagt ctttttgagt
     7561 tagaaaacgg tcgtaaacgg atgctggcta gtgccggaga attacaaaaa ggaaatgagc
     7621 tggctctgcc aagcaaatat gtgaattttt tatatttagc tagtcattat gaaaagttga
     7681 agggtagtcc agaagataac gaacaaaaac aattgtttgt ggagcagcat aagcattatt
     7741 tagatgagat tattgagcaa atcagtgaat tttctaagcg tgttatttta gcagatgcca
     7801 atttagataa agttcttagt gcatataaca aacatagaga caaaccaata cgtgaacaag
     7861 cagaaaatat tattcattta tttacgttga cgaatcttgg agctcccgct gcttttaaat
     7921 attttgatac aacaattgat cgtaaacgat atacgtctac aaaagaagtt ttagatgcca
     7981 ctcttatcca tcaatccatc actggtcttt atgaaacacg cattgatttg agtcagctag
     8041 gaggtgacgg tggagggcca aaaaagaaaa gaaaagttga agatgcttct gctcatcatc
     8101 accatcacca tcaccattaa atccgctcta accgaaaagg aaggagttag acaacctgaa
     8161 gtctaggtcc ctatttattt ttttatagtt atgttagtat taagaacgtt atttatattt
     8221 caaatttttc ttttttttct gtacagacgc gtgtacgcat gtaacattat actgaaaacc
     8281 ttgcttgaga aggttttggg acgctcgaag tgagcaaaag gccagcaaaa ggccaggaac
     8341 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     8401 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     8461 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     8521 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     8581 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     8641 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     8701 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     8761 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     8821 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     8881 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     8941 aaaaaaggat ctcaagaaga tcctttgatc ttttctaccg aagcatctgt gcttcatttt
     9001 gtagaacaaa aatgcaacgc gagagcgcta atttttcaaa caaagaatct gagctgcatt
     9061 tttacagaac agaaatgcaa cgcgaaagcg ctattttacc aacgaagaat ctgtgcttca
     9121 tttttgtaaa acaaaaatgc aacgcgagag cgctaatttt tcaaacaaag aatctgagct
     9181 gcatttttac agaacagaaa tgcaacgcga gagcgctatt ttaccaacaa agaatctata
     9241 cttctttttt gttctacaaa aatgcatccc gagagcgcta tttttctaac aaagcatctt
     9301 agattacttt ttttctcctt tgtgcgctct ataatgcagt ctcttgataa ctttttgcac
     9361 tgtaggtccg ttaaggttag aagaaggcta ctttggtgtc tattttctct tccataaaaa
     9421 aagcctgact ccacttcccg cgtttactga ttactagcga agctgcgggt gcattttttc
     9481 aagataaagg catccccgat tatattctat accgatgtgg attgcgcata ctttgtgaac
     9541 agaaagtgat agcgttgatg attcttcatt ggtcagaaaa ttatgaacgg tttcttctat
     9601 tttgtctcta tatactacgt ataggaaatg tttacatttt cgtattgttt tcgattcact
     9661 ctatgaatag ttcttactac aatttttttg tctaaagagt aatactagag ataaacataa
     9721 aaaatgtaga ggtcgagttt agatgcaagt tcaaggagcg aaaggtggat gggtaggtta
     9781 tatagggata tagcacagag atatatagca aagagatact tttgagcaat gtttgtggaa
     9841 gcggtattcg caatatttta gtagcccgtt acagtccggt gcgtttttgg ttttttgaaa
     9901 gtgcgtcttc agagcgcttt tggttttcaa aagcgctctg aagttcctat actttctaga
     9961 gaataggaac ttcggaatag gaacttcaaa gcgtttccga aaacgagcgc ttccgaaaat
    10021 gcaacgcgag ctgcgcacat acagctcact gttcacgtcg cacctatatc tgcgtgttgc
    10081 ctgtatatat atatacatga gaagaacggc atagtgcgtg tttatgctta aatgc
//
