LOCUS       sequence-464604-       10133 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast CRISPR plasmid targeting the natMX cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10133)
  AUTHORS   Soreanu I, Hendler A, Dahan D, Dovrat D, Aharoni A
  TITLE     Marker-free genetic manipulations in yeast using CRISPR/CAS9 system.
  JOURNAL   Curr Genet. 2018 Oct;64(5):1129-1139. doi: 
            10.1007/s00294-018-0831-y. Epub 2018 Apr 6.
  PUBMED    29626221
REFERENCE   2  (bases 1 to 10133)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s00294-018-0831-y"; journalName: "Curr Genet"; date: 
            "2018-10"; volume: "64"; issue: "5"; pages: "1129-1139"
COMMENT     Sequence Label: pCY5n
FEATURES             Location/Qualifiers
     source          1..10133
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_RNA        225..300
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        400..613
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             614..1417
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     primer_bind     complement(1685..1706)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1794..1898
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1899..1967,1968..2759)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2117..2136)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     misc_RNA        3072..3147
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             3943..8046
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             3943..8046
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             8056..8076
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8092..8115
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     terminator      8119..8308
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     rep_origin      complement(8365..8953)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8445..8464)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     rep_origin      8975..9957
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(9938..9985)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
ORIGIN
        1 gtatatgtgt tatgtagtat actctttctt caacaattaa atactctcgg tagccaagtt
       61 ggtttaaggc gcaagactgt aatttatcac tacgaaatct tgagatcggg cgttcgactc
      121 gcccccggga gagatggccg gcatggtccc agcctcctcg ctggcgccgg ctgggcaaca
      181 ccttcgggtg gcgaatggga ctttgtaccg caccagtgtc ccgggtttta gagctagaaa
      241 tagcaagtta aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc
      301 tttttttatt ttttgtcact attgttatgt aaaatgccac ctctgacagt atggaacgca
      361 aacttctgtc tagtggatat aggtctagag atctaccggt tcaattcatc atttttttta
      421 ttcttttttt tgatttcggt ttccttgaaa tttttttgat tcggtaatct ccgaacagaa
      481 ggaagaacga aggaaggagc acagacttag attggtatat atacgcatat gtagtgttga
      541 agaaacatga aattgcccag tattcttaac ccaactgcac agaacaaaaa cctgcaggaa
      601 acgaagataa atcatgtcga aagctacata taaggaacgt gctgctactc atcctagtcc
      661 tgttgctgcc aagctattta atatcatgca cgaaaagcaa acaaacttgt gtgcttcatt
      721 ggatgttcgt accaccaagg aattactgga gttagttgaa gcattaggtc ccaaaatttg
      781 tttactaaaa acacatgtgg atatcttgac tgatttttcc atggagggca cagttaagcc
      841 gctaaaggca ttatccgcca agtacaattt tttactcttc gaagacagaa aatttgctga
      901 cattggtaat acagtcaaat tgcagtactc tgcgggtgta tacagaatag cagaatgggc
      961 agacattacg aatgcacacg gtgtggtggg cccaggtatt gttagcggtt tgaagcaggc
     1021 ggcagaagaa gtaacaaagg aacctagagg ccttttgatg ttagcagaat tgtcatgcaa
     1081 gggctcccta tctactggag aatatactaa gggtactgtt gacattgcga agagcgacaa
     1141 agattttgtt atcggcttta ttgctcaaag agacatgggt ggaagagatg aaggttacga
     1201 ttggttgatt atgacacccg gtgtgggttt agatgacaag ggagacgcat tgggtcaaca
     1261 gtatagaacc gtggatgatg tggtctctac aggatctgac attattattg ttggaagagg
     1321 actatttgca aagggaaggg atgctaaggt agagggtgaa cgttacagaa aagcaggctg
     1381 ggaagcatat ttgagaagat gcggccagca aaactaaaaa actgtattat aagtaaatgc
     1441 atgtatacta aactcacaaa ttagagcttc aatttaatta tatcagttat taccctatgc
     1501 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggaaa ttgtaagcgt
     1561 taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata
     1621 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt
     1681 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
     1741 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccgcggc cgccgcggaa
     1801 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     1861 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     1921 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     1981 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     2041 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     2101 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     2161 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     2221 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     2281 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     2341 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     2401 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     2461 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     2521 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     2581 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     2641 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     2701 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     2761 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     2821 aaaggatcta ggtgaagatc ctttttgata atcttaatta agtatatgtg ttatgtagta
     2881 tactctttct tcaacaatta aatactctcg gtagccaagt tggtttaagg cgcaagactg
     2941 taatttatca ctacgaaatc ttgagatcgg gcgttcgact cgcccccggg agagatggcc
     3001 ggcatggtcc cagcctcctc gctggcgccg gctgggcaac accttcgggt ggcgaatggg
     3061 actttgttaa cgttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa
     3121 cttgaaaaag tggcaccgag tcggtgcttt ttttattttt tgtcactatt gttatgtaaa
     3181 atgccacctc tgacagtatg gaacgcaaac ttctgtctag tggatactcg agaaccctta
     3241 atagtcgaac aagaagcagg caaagtttag agcactgccc ctccgcactc aaaaaagaaa
     3301 aaactaggag gaaaataaaa ttctcaacca cacaaacaca taaacacata caaatacaaa
     3361 tacaagctta tttacttgac atcgcgcgat cttccactat tcagcgccgt ccgccctctc
     3421 tcgtgttttt tgtttacgcg acaactatgc gaaatccgga gcaacgggca accgtttggg
     3481 gaaagaccac acccacgcgc gatcgccatg gcaacgaggt cgcacacgcc ccacacccag
     3541 acctccctgc gagcgggcat gggtacaatg tccccgttgc cacagacacc acttcgtagc
     3601 acagcgcaga gcgtagcgtg ttgttgctgc tgacaaaaga aaatttttct tagcaaagca
     3661 aaggagggga agcacgggca gatagcaccg taccataccc ttggaaactc gaaatgaacg
     3721 aagcaggaaa tgagagaatg agagttttgt aggtatatat agcggtagtg tttgcgcgtt
     3781 accatcatct tctggatcta tctattgttc ttttcctcat cactttcccc tttttcgctc
     3841 ttcttcttgt cttttatttc tttctttttt ttaattgttc cctcgattgg ctatctacca
     3901 aagaatccaa acttaataca cgtatttatt tgtccaatta ccatggataa gaaatactca
     3961 ataggcttag atatcggcac aaatagcgtc ggatgggcgg tgatcactga tgaatataag
     4021 gttccgtcta aaaagttcaa ggttctggga aatacagacc gccacagtat caaaaaaaat
     4081 cttatagggg ctcttttatt tgacagtgga gagacagcgg aagcgactcg tctcaaacgg
     4141 acagctcgta gaaggtatac acgtcggaag aatcgtattt gttatctaca ggagattttt
     4201 tcaaatgaga tggcgaaagt agatgatagt ttctttcatc gacttgaaga gtcttttttg
     4261 gtggaagaag acaagaagca tgaacgtcat cctatttttg gaaatatagt agatgaagtt
     4321 gcttatcatg agaaatatcc aactatctat catctgcgaa aaaaattggt agattctact
     4381 gataaagcgg atttgcgctt aatctatttg gccttagcgc atatgattaa gtttcgtggt
     4441 cattttttga ttgagggaga tttaaatcct gataatagtg atgtggacaa actatttatc
     4501 cagttggtac aaacctacaa tcaattattt gaagaaaacc ctattaacgc aagtggagta
     4561 gatgctaaag cgattctttc tgcacgattg agtaaatcaa gacgattaga aaatctcatt
     4621 gctcagctcc ccggtgagaa gaaaaatggc ttatttggga atctcattgc tttgtcattg
     4681 ggtttgaccc ctaattttaa atcaaatttt gatttggcag aagatgctaa attacagctt
     4741 tcaaaagata cttacgatga tgatttagat aatttattgg cgcaaattgg agatcaatat
     4801 gctgatttgt ttttggcagc taagaattta tcagatgcta ttttactttc agatatccta
     4861 agagtaaata ctgaaataac taaggctccc ctatcagctt caatgattaa acgctacgat
     4921 gaacatcatc aagacttgac tcttttaaaa gctttagttc gacaacaact tccagaaaag
     4981 tataaagaaa tcttttttga tcaatcaaaa aacggatatg caggttatat tgatggggga
     5041 gctagccaag aagaatttta taaatttatc aaaccaattt tagaaaaaat ggatggtact
     5101 gaggaattat tggtgaaact aaatcgtgaa gatttgctgc gcaagcaacg gacctttgac
     5161 aacggctcta ttccccatca aattcacttg ggtgagctgc atgctatttt gagaagacaa
     5221 gaagactttt atccattttt aaaagacaat cgtgagaaga ttgaaaaaat cttgactttt
     5281 cgaattcctt attatgttgg tccattggcg cgtggcaata gtcgttttgc atggatgact
     5341 cggaagtctg aagaaacaat taccccatgg aattttgaag aagttgtcga taaaggtgct
     5401 tcagctcaat catttattga acgcatgaca aactttgata aaaatcttcc aaatgaaaaa
     5461 gtactaccaa aacatagttt gctttatgag tattttacgg tttataacga attgacaaag
     5521 gtcaaatatg ttactgaagg aatgcgaaaa ccagcatttc tttcaggtga acagaagaaa
     5581 gccattgttg atttactctt caaaacaaat cgaaaagtaa ccgttaagca attaaaagaa
     5641 gattatttca aaaaaataga atgttttgat agtgttgaaa tttcaggagt tgaagataga
     5701 tttaatgctt cattaggtac ctaccatgat ttgctaaaaa ttattaaaga taaagatttt
     5761 ttggataatg aagaaaatga agatatctta gaggatattg ttttaacatt gaccttattt
     5821 gaagataggg agatgattga ggaaagactt aaaacatatg ctcacctctt tgatgataag
     5881 gtgatgaaac agcttaaacg tcgccgttat actggttggg gacgtttgtc tcgaaaattg
     5941 attaatggta ttagggataa gcaatctggc aaaacaatat tagatttttt gaaatcagat
     6001 ggttttgcca atcgcaattt tatgcagctg atccatgatg atagtttgac atttaaagaa
     6061 gacattcaaa aagcacaagt gtctggacaa ggcgatagtt tacatgaaca tattgcaaat
     6121 ttagctggta gccctgctat taaaaaaggt attttacaga ctgtaaaagt tgttgatgaa
     6181 ttggtcaaag taatggggcg gcataagcca gaaaatatcg ttattgaaat ggcacgtgaa
     6241 aatcagacaa ctcaaaaggg ccagaaaaat tcgcgagagc gtatgaaacg aatcgaagaa
     6301 ggtatcaaag aattaggaag tcagattctt aaagagcatc ctgttgaaaa tactcaattg
     6361 caaaatgaaa agctctatct ctattatctc caaaatggaa gagacatgta tgtggaccaa
     6421 gaattagata ttaatcgttt aagtgattat gatgtcgatc acattgttcc acaaagtttc
     6481 cttaaagacg attcaataga caataaggtc ttaacgcgtt ctgataaaaa tcgtggtaaa
     6541 tcggataacg ttccaagtga agaagtagtc aaaaagatga aaaactattg gagacaactt
     6601 ctaaacgcca agttaatcac tcaacgtaag tttgataatt taacgaaagc tgaacgtgga
     6661 ggtttgagtg aacttgataa agctggtttt atcaaacgcc aattggttga aactcgccaa
     6721 atcactaagc atgtggcaca aattttggat agtcgcatga atactaaata cgatgaaaat
     6781 gataaactta ttcgagaggt taaagtgatt accttaaaat ctaaattagt ttctgacttc
     6841 cgaaaagatt tccaattcta taaagtacgt gagattaaca attaccatca tgcccatgat
     6901 gcgtatctaa atgccgtcgt tggaactgct ttgattaaga aatatccaaa acttgaatcg
     6961 gagtttgtct atggtgatta taaagtttat gatgttcgta aaatgattgc taagtctgag
     7021 caagaaatag gcaaagcaac cgcaaaatat ttcttttact ctaatatcat gaacttcttc
     7081 aaaacagaaa ttacacttgc aaatggagag attcgcaaac gccctctaat cgaaactaat
     7141 ggggaaactg gagaaattgt ctgggataaa gggcgagatt ttgccacagt gcgcaaagta
     7201 ttgtccatgc cccaagtcaa tattgtcaag aaaacagaag tacagacagg cggattctcc
     7261 aaggagtcaa ttttaccaaa aagaaattcg gacaagctta ttgctcgtaa aaaagactgg
     7321 gatccaaaaa aatatggtgg ttttgatagt ccaacggtag cttattcagt cctagtggtt
     7381 gctaaggtgg aaaaagggaa atcgaagaag ttaaaatccg ttaaagagtt actagggatc
     7441 acaattatgg aaagaagttc ctttgaaaaa aatccgattg actttttaga agctaaagga
     7501 tataaggaag ttaaaaaaga cttaatcatt aaactaccta aatatagtct ttttgagtta
     7561 gaaaacggtc gtaaacggat gctggctagt gccggagaat tacaaaaagg aaatgagctg
     7621 gctctgccaa gcaaatatgt gaatttttta tatttagcta gtcattatga aaagttgaag
     7681 ggtagtccag aagataacga acaaaaacaa ttgtttgtgg agcagcataa gcattattta
     7741 gatgagatta ttgagcaaat cagtgaattt tctaagcgtg ttattttagc agatgccaat
     7801 ttagataaag ttcttagtgc atataacaaa catagagaca aaccaatacg tgaacaagca
     7861 gaaaatatta ttcatttatt tacgttgacg aatcttggag ctcccgctgc ttttaaatat
     7921 tttgatacaa caattgatcg taaacgatat acgtctacaa aagaagtttt agatgccact
     7981 cttatccatc aatccatcac tggtctttat gaaacacgca ttgatttgag tcagctagga
     8041 ggtgacggtg gagggccaaa aaagaaaaga aaagttgaag atgcttctgc tcatcatcac
     8101 catcaccatc accattaaat ccgctctaac cgaaaaggaa ggagttagac aacctgaagt
     8161 ctaggtccct atttattttt ttatagttat gttagtatta agaacgttat ttatatttca
     8221 aatttttctt ttttttctgt acagacgcgt gtacgcatgt aacattatac tgaaaacctt
     8281 gcttgagaag gttttgggac gctcgaagtg agcaaaaggc cagcaaaagg ccaggaaccg
     8341 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     8401 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     8461 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     8521 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     8581 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     8641 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     8701 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     8761 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     8821 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     8881 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     8941 aaaaggatct caagaagatc ctttgatctt ttctaccgaa gcatctgtgc ttcattttgt
     9001 agaacaaaaa tgcaacgcga gagcgctaat ttttcaaaca aagaatctga gctgcatttt
     9061 tacagaacag aaatgcaacg cgaaagcgct attttaccaa cgaagaatct gtgcttcatt
     9121 tttgtaaaac aaaaatgcaa cgcgagagcg ctaatttttc aaacaaagaa tctgagctgc
     9181 atttttacag aacagaaatg caacgcgaga gcgctatttt accaacaaag aatctatact
     9241 tcttttttgt tctacaaaaa tgcatcccga gagcgctatt tttctaacaa agcatcttag
     9301 attacttttt ttctcctttg tgcgctctat aatgcagtct cttgataact ttttgcactg
     9361 taggtccgtt aaggttagaa gaaggctact ttggtgtcta ttttctcttc cataaaaaaa
     9421 gcctgactcc acttcccgcg tttactgatt actagcgaag ctgcgggtgc attttttcaa
     9481 gataaaggca tccccgatta tattctatac cgatgtggat tgcgcatact ttgtgaacag
     9541 aaagtgatag cgttgatgat tcttcattgg tcagaaaatt atgaacggtt tcttctattt
     9601 tgtctctata tactacgtat aggaaatgtt tacattttcg tattgttttc gattcactct
     9661 atgaatagtt cttactacaa tttttttgtc taaagagtaa tactagagat aaacataaaa
     9721 aatgtagagg tcgagtttag atgcaagttc aaggagcgaa aggtggatgg gtaggttata
     9781 tagggatata gcacagagat atatagcaaa gagatacttt tgagcaatgt ttgtggaagc
     9841 ggtattcgca atattttagt agcccgttac agtccggtgc gtttttggtt ttttgaaagt
     9901 gcgtcttcag agcgcttttg gttttcaaaa gcgctctgaa gttcctatac tttctagaga
     9961 ataggaactt cggaatagga acttcaaagc gtttccgaaa acgagcgctt ccgaaaatgc
    10021 aacgcgagct gcgcacatac agctcactgt tcacgtcgca cctatatctg cgtgttgcct
    10081 gtatatatat atacatgaga agaacggcat agtgcgtgtt tatgcttaaa tgc
//
