LOCUS       sequence-464607-       12798 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid is for the production of lentiviral vectors expressing 
            an active form of Glis3 together with GFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12798)
  TITLE     Lentiviral plasmid for expressing a constitutively active form of 
            Glis3 transcription factor
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pWPI-Glis3M412
FEATURES             Location/Qualifiers
     source          1..12798
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1299..1532
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1717..1761
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2144..3322
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2375..3313
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3270..3290
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    3370..3487
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    3519..3543
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5183..5207)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    5231..5798
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5249..5800
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5393..5410)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5620..5639
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(5803..5805,5806..5808,5809..6519)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5803..5805,5806..5808,5809..6519)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5848..5869)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6109..6128)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6456..6477
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(6598..6614)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6599..6615)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     misc_feature    6628..7216
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6681..6701)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7099..7110)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(7360..7393)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             7413..7593
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(7618..7636)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(7619..7636)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7926..7945)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(8159..8178)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8278..8300
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8338..8356)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8424..8528
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8529..8597,8598..9389)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8747..8766)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9560..10148
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10049..10068
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10302..10319
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10389..10409)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10394..10723
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10574..10709
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          11857..11922
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             12052..12072
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    12497..12631
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12534..12553)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12588..12607
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     1201 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     1261 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     1321 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     1381 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     1441 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     1501 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     1561 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     1621 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     1681 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     1741 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     1801 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     1861 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     1921 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
     1981 gacagatcca ttcgattagt gaacggatct cgacggtatc gatcacgaga ctagcctcga
     2041 ccatcgatgt cgacgataag ctttgcaaag atggataaag ttttaaacag agaggaatct
     2101 ttgcagctaa tggaccttct aggtcttgaa aggagtggga attggctccg gtgcccgtca
     2161 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg
     2221 aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct
     2281 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt
     2341 tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg
     2401 gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca cctggctgca
     2461 gtacgtgatt cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg
     2521 cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg
     2581 ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc
     2641 catttaaaat ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa
     2701 tgcgggccaa gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc
     2761 ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat
     2821 cggacggggg tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg
     2881 tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag
     2941 atggccgctt cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga
     3001 gcgggcgggt gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc
     3061 atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg
     3121 gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga
     3181 gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc
     3241 cctttttgag tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt
     3301 tcttccattt caggtgtcgt gaggaatttc gacatttaaa tttaattaat ctcgacggta
     3361 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
     3421 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca
     3481 aaattttatc gatcacgaga ctagcctcga ggtttatcac aagtttgtac aaaaaagcag
     3541 gctccaccat gggaaccaat tcagtcgact ggatccggta ccgaattgat ccaccatggt
     3601 gttgcagcct ggcctaccgg gccaggatgg ccagacagcc aacatgctca aaacagagcg
     3661 cctggaggag ttccccgcca gtgccttgga cctgccctct gctctgcctc tccctcttcc
     3721 tccgcctcag ggtcccccac ccccatacca tgcccatcca caccttcatc acccagagct
     3781 cctgcctcac acccaatcgc tgtccctggc ccagactggc ctggaagagg atggggagat
     3841 ggaagactca ggggggaagc actgctgccg ttggatagac tgcagtgccc tttatgacca
     3901 gcaggaggaa ctggtgaggc acatcgagaa ggtccacata gaccaacgca agggggaaga
     3961 cttcacgtgc ttctggactg gctgccctag aagatacaag cctttcaacg cacggtatag
     4021 actgctgatc cacatgaggg tccactctgg ggagaagccc aacaagtgta cgttcgaagg
     4081 ctgcaagaag gccttttcca ggctcgagaa cctcaagatt cacttgcgga gccacacggg
     4141 tgagaagcca tacttgtgcc agcatccggg ctgccaaaag gccttcagca attccagtga
     4201 ccgtgccaag caccagagga cacatcttga cactaaacct tatgcttgtc aaattccagg
     4261 atgtacaaag cgctacacag acccaagctc cctcagaaag cacgtgaagg cacattcttc
     4321 ccgagagcag caagcaagga aaaagctacg gtctagcact gagctccatc cagatctcct
     4381 cacagactgc ctcgctgtgc agcccctgca gccagccacg tcccctggag atgctgctga
     4441 tcataccgtg gggcactccc cagggccggg gcctgggcca gggcctgggg ctgaactcta
     4501 ttcagctccc attttcgcca gcaatcattc aactcgaagt ggagcagctg ctggggctgg
     4561 gccaccccca catcctgtca gtcgcccttc tccaggacat aatgtacagg ggagccccca
     4621 caacccctcc tcccagttac ctccactcac agctgtggac gcaggcgctg agaggtttgc
     4681 acctccgact ccatctcctc accacatcag cccgggaaga gttccagctc ctccgtcact
     4741 gctccagaga gcacaggctc cccactccca gcagccacca ggctcactcc tgaagcccta
     4801 ccagcctgaa accaactctt cttttcagcc aaatggtatc catgtccacg gattttatgg
     4861 ccagctgcag acattctgtc ccccgcatta tcctgattcc caaagaactg tgccacccag
     4921 cggctcctgc agtatggtgc cttcctttga ggactgcctg gtccccacgt ccatgggtca
     4981 agccggtttt gatgttttcc acagagcctt ctcaacacac tcaggcatca cagtgtacga
     5041 tttgccttca gcttcctcaa gcctctttgg ggaatctctt cgcagtggcc ctgaagaccc
     5101 cacgttcttg cagctcagtg ctgtggaccg ctgtcctagc cagctctcct ctgtgtatac
     5161 cgaaggctga gcggccatct agacccagct ttcttgtaca aagtggtgat aaactacggg
     5221 ctgcaggaat tccgcccccc cccccctaac gttactggcc gaagccgctt ggaataaggc
     5281 cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg
     5341 gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc
     5401 aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga
     5461 agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg
     5521 tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag
     5581 tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc
     5641 aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct
     5701 cggtgcacat gctttacatg tgtttagtcg aggttaaaaa acgtctaggc cccccgaacc
     5761 acggggacgt ggttttcctt tgaaaaacac gatgataata ccatggtgag caagggcgag
     5821 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
     5881 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
     5941 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc
     6001 tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag
     6061 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
     6121 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
     6181 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
     6241 aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc
     6301 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
     6361 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc
     6421 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     6481 gccgccggga tcactctcgg catggacgag ctgtacaagt ccggactcag atctcgacta
     6541 gctagtagct agctagctag tcgagctcaa gcttcgaatt cgatatcaag cttatcgcga
     6601 taccgtcgac ctcgagggaa ttccgataat caacctctgg attacaaaat ttgtgaaaga
     6661 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     6721 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     6781 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     6841 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     6901 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     6961 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     7021 aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     7081 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     7141 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     7201 tgggccgcct ccccgcatcg ggaattcgag ctcggtacct ttaagaccaa tgacttacaa
     7261 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     7321 ctcccaacga agacaagatg ggatcaattc accatgggaa taacttcgta tagcatacat
     7381 tatacgaagt tatgctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
     7441 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     7501 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     7561 gaccctttta gtcagtgtgg aaaatctcta gcagcatcta gaattaattc cgtgtattct
     7621 atagtgtcac ctaaatcgta tgtgtatgat acataaggtt atgtattaat tgtagccgcg
     7681 ttctaacgac aatatgtaca agcctaattg tgtagcatct ggcttactga agcagaccct
     7741 atcatctctc tcgtaaactg ccgtcagagt cggtttggtt ggacgaacct tctgagtttc
     7801 tggtaacgcc gtcccgcacc cggaaatggt cagcgaacca atcagcaggg tcatcgctag
     7861 ccagatcctc tacgccggac gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc
     7921 tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg gctcgccact tcgggctcat
     7981 gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg gccgggggac tgttgggcgc
     8041 catctccttg catgcaccat tccttgcggc ggcggtgctc aacggcctca acctactact
     8101 gggctgcttc ctaatgcagg agtcgcataa gggagagcgt cgaatggtgc actctcagta
     8161 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
     8221 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
     8281 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc
     8341 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     8401 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     8461 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     8521 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     8581 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     8641 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     8701 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     8761 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     8821 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     8881 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     8941 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     9001 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     9061 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     9121 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     9181 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     9241 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     9301 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     9361 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     9421 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     9481 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     9541 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     9601 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     9661 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     9721 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     9781 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     9841 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     9901 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     9961 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
    10021 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
    10081 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
    10141 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
    10201 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
    10261 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
    10321 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
    10381 gcagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
    10441 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
    10501 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
    10561 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
    10621 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
    10681 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagcttg gacacaagac
    10741 aggcttgcga gatatgtttg agaataccac tttatcccgc gtcagggaga ggcagtgcgt
    10801 aaaaagacgc ggactcatgt gaaatactgg tttttagtgc gccagatctc tataatctcg
    10861 cgcaacctat tttcccctcg aacacttttt aagccgtaga taaacaggct gggacacttc
    10921 acatgagcga aaaatacatc gtcacctggg acatgttgca gatccatgca cgtaaactcg
    10981 caagccgact gatgccttct gaacaatgga aaggcattat tgccgtaagc cgtggcggtc
    11041 tgtaccgggt gcgttactgg cgcgtgaact gggtattcgt catgtcgata ccgtttgtat
    11101 ttccagctac gatcacgaca accagcgcga gcttaaagtg ctgaaacgcg cagaaggcga
    11161 tggcgaaggc ttcatcgtta ttgatgacct ggtggatacc ggtggtactg cggttgcgat
    11221 tcgtgaaatg tatccaaaag cgcactttgt caccatcttc gcaaaaccgg ctggtcgtcc
    11281 gctggttgat gactatgttg ttgatatccc gcaagatacc tggattgaac agccgtggga
    11341 tatgggcgtc gtattcgtcc cgccaatctc cggtcgctaa tcttttcaac gcctggcact
    11401 gccgggcgtt gttcttttta acttcaggcg ggttacaata gtttccagta agtattctgg
    11461 aggctgcatc catgacacag gcaaacctga gcgaaaccct gttcaaaccc cgctttaaac
    11521 atcctgaaac ctcgacgcta gtccgccgct ttaatcacgg cgcacaaccg cctgtgcagt
    11581 cggcccttga tggtaaaacc atccctcact ggtatcgcat gattaaccgt ctgatgtgga
    11641 tctggcgcgg cattgaccca cgcgaaatcc tcgacgtcca ggcacgtatt gtgatgagcg
    11701 atgccgaacg taccgacgat gatttatacg atacggtgat tggctaccgt ggcggcaact
    11761 ggatttatga gtgggccccg gatctttgtg aaggaacctt acttctgtgg tgtgacataa
    11821 ttggacaaac tacctacaga gatttaaagc tctaaggtaa atataaaatt tttaagtgta
    11881 taatgtgtta aactactgat tctaattgtt tgtgtatttt agattccaac ctatggaact
    11941 gatgaatggg agcagtggtg gaatgccttt aatgaggaaa acctgttttg ctcagaagaa
    12001 atgccatcta gtgatgatga ggctactgct gactctcaac attctactcc tccaaaaaag
    12061 aagagaaagg tagaagaccc caaggacttt ccttcagaat tgctaagttt tttgagtcat
    12121 gctgtgttta gtaatagaac tcttgcttgc tttgctattt acaccacaaa ggaaaaagct
    12181 gcactgctat acaagaaaat tatggaaaaa tattctgtaa cctttataag taggcataac
    12241 agttataatc ataacatact gttttttctt actccacaca ggcatagagt gtctgctatt
    12301 aataactatg ctcaaaaatt gtgtaccttt agctttttaa tttgtaaagg ggttaataag
    12361 gaatatttga tgtatagtgc cttgactaga gatcataatc agccatacca catttgtaga
    12421 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
    12481 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
    12541 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
    12601 actcatcaat gtatcttatc atgtctggat caactggata actcaagcta accaaaatca
    12661 tcccaaactt cccaccccat accctattac cactgccaat tacctgtggt ttcatttact
    12721 ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt gttaaatatg
    12781 tactacaaac ttagtagt
//
